| Definition | Burkholderia cenocepacia HI2424 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008542 |
| Length | 3,483,902 |
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The map label for this gene is mrcA [H]
Identifier: 116688411
GI number: 116688411
Start: 431515
End: 433908
Strand: Reverse
Name: mrcA [H]
Synonym: Bcen2424_0387
Alternate gene names: 116688411
Gene position: 433908-431515 (Counterclockwise)
Preceding gene: 116688424
Following gene: 116688409
Centisome position: 12.45
GC content: 66.96
Gene sequence:
>2394_bases ATGCCTATGCAATCCACGACTCCTACGTCCCCGCCTCCCGCCCCGGAGCCGAAGAAGCGGCCCTGGTGGCAAAAAGTCCT GCTCGGCTTCGCCGCGATGTGCGTGGCCCTCGTCGTGGCCGGCGGCCTCGTGCTCGGCTACGCGCTCGTCGTGGCCTGGC CGAACATGCCGTCGCTCGACGCGCTGACCGACTACCGCCCGAAGGTGCCGCTGCGCATCTATACGTCCGATCATGTGCTG ATCGGCGAATTCGGCGAGGAGCGCCGCGACGTCGTCCATTTCAAGGACGTGCCCGACTCGCTGAAGAAGGCGATTCTCGC GATCGAGGACGCACGCTTCTACGATCACGGCGGCGTCGACCTCACCGGCATCATCCGCGCCGGCTTCGTCGCGCTGACGA ACGGCCACGCGTCGCAGGGCGCGAGCACGATCACGATGCAGGTCGCGCGCAACTTCTTCCTGTCGAGCGAGAAGACCTAC ACGCGCAAGATCTACGAGATGCTGCTCGCGTACCGGATCGAGCGCGCGCTGACGAAGGATCAGATTCTCGAGGTCTACAT GAATCAGATCTATCTCGGCCAGCGCGCGTACGGCTTCGCGAGCGCCGCGCGGGTCTATTTCGGCAAGGACCTGAAGGACA TCACGCTCGCGGAAGCCGCAATGCTCGCGGGGCTGCCGAAGGCGCCGTCCGCGTATAACCCGGTCGTCAACCCGAAGCGC GCGAAGGTGCGCCAGGAATACATCCTGCAGCGGATGCTCGAGCTGAACTTCATCACGCGCGAGCAGTACGACGAAGCCGC CGCGCAGCCGCTCGTCGTCAAGGGGCCGGGCCGCGATTTCAGCGTGCACGCCGAGTACGTCGCGGAAATGGTCAGACAGA TGATGTACGCGCAGTATCGCGAGGAAACCTACACGCGCGGCTTCAACGTCGTCACGACGATCGATTCCGCGGACCAGCAG GTCGCGTACACCGCGCTGCGCAAGGGCATCATGGATTACGAGCGCCGCCACGGCTATCGCGGGCCGGAAGGCTTCATCGA GCTGCCGGCCGGCGCCGACGATCGCGAACAGGCGATCGACGACGCGCTGCTCGAGCATCCCGACAACGGCGAGCTGATCG CCGCGGTCGTGACGGCCGCGAGCCCGCGCCAGATCACGGTGGCGTTCATCGACGGCAGCACGGCGACGATCGAGGGCGAC AACCTGCGCTTCGCGTCGGGCGCGCTGTCGACCAACGCGCAGCCAAACCGCCGCATCCGCCCCGGCGCGATCGTCCGCGT CGTGAAGAACGACGCCGGCAAATGGTCGATCACGCAGTTGCCGCAGGTCGAAGGCGCGTTCCTGTCGATCGTCCCGCAGG ACGGGGCGATCCGCTCGCTCGTCGGCGGCTTCGACTACAACAAGAACAAGTTCAACCACGTCACGCAGGCGTGGCGGCAA CCGGGTTCGTCGTTCAAGCCGTTCATCTACTCGGCCTCGCTCGACAAGGGCCTCGGCCCGGCGACCGTCATCAACGACGG CCCGCTGTATTTCAGCGCCGCGGAAACGGGCGGCCAGCCGTGGGAGCCGAAGAACTACGGCGGCGGCTTCGAGGGCCCGA TGTCGATGCGCACCGCGCTGCAGCGCTCGCGCAACCTCGTGTCGATCCGGATTCTCAACAACATCGGCACGAAATACGCG CAGCAATACATCACGCGCTTCGGCTTCGACGCCGATCGCCATCCGGCCTACCTGCCGATGGCGCTCGGCGCCGGCCAGGT CACGCCGCTGCAGATGGCCGGCGCGTACTCGGTCTTCGCGAACGGCGGCTATCGCGTCAATCCGTACCTGATCGCCGAGG TCACCGATCCGAACGGCGCGATCGTCGCGCGCGCGCAGCCGCTCGTCGCGGAGCAGAACGCGCCGCGCGCGATCGACGCA CGCAACGCCTACGTGATGAACAGCCTGCTGCAGAGCGTCGCGCAGCGCGGCACGGGCGCACGGACCAACGTGCTCAAGCG CACCGACCTCGCGGGCAAGACCGGCACGACGAACGACTCGCACGACGCGTGGTTCGCCGGCTACCAGCACACGCTCGCCG CAATCGCGTGGATCGGCTACGACAATCCGCGCAGCCTCGGCGATCGCGAAACCGGCGGCGGCCTGTCACTGCCGGTCTGG ATCGAGTACATGGGTGCGGCGCTGAAGGGCGTGCCCGAGTTCAAGCCGACGGTGCCGGACGGTGTCGAATCGCTCGGCGG CGAGCTGTACTTCACCGAGTTCACGCCGGGGCACGGCTTCGTGTCGACGGTCGGCGTGCCGCAGGCGCCGGCCGCGCAAA ACGTCGACGAGGCCGTGCCGCACGTCGACGAGCAGGAAAAGCAGGACATCATGAACCTGTTCCGCGGCCACTGA
Upstream 100 bases:
>100_bases ACTTATGCTGACGGCACGCCGACCGCGGCTTTCCGACGGGCGGCCCCCGCGTCAGGACCGGTCTTACCGGGCAGCTATAA TCGCGGGACTGTTTTCCGGT
Downstream 100 bases:
>100_bases CGCTCGGCCCGGCCATGAAAAAAGCGCCCCGCGGGGCGCTTTTTCTTTGGGCACGACGCGCGGAGCGCCGCGCCCCGGCC GTCACGCGCTGAACGTGATC
Product: 1A family penicillin-binding protein
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 797; Mature: 796
Protein sequence:
>797_residues MPMQSTTPTSPPPAPEPKKRPWWQKVLLGFAAMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVL IGEFGEERRDVVHFKDVPDSLKKAILAIEDARFYDHGGVDLTGIIRAGFVALTNGHASQGASTITMQVARNFFLSSEKTY TRKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR AKVRQEYILQRMLELNFITREQYDEAAAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYREETYTRGFNVVTTIDSADQQ VAYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSTATIEGD NLRFASGALSTNAQPNRRIRPGAIVRVVKNDAGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNNIGTKYA QQYITRFGFDADRHPAYLPMALGAGQVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVARAQPLVAEQNAPRAIDA RNAYVMNSLLQSVAQRGTGARTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVW IEYMGAALKGVPEFKPTVPDGVESLGGELYFTEFTPGHGFVSTVGVPQAPAAQNVDEAVPHVDEQEKQDIMNLFRGH
Sequences:
>Translated_797_residues MPMQSTTPTSPPPAPEPKKRPWWQKVLLGFAAMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVL IGEFGEERRDVVHFKDVPDSLKKAILAIEDARFYDHGGVDLTGIIRAGFVALTNGHASQGASTITMQVARNFFLSSEKTY TRKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR AKVRQEYILQRMLELNFITREQYDEAAAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYREETYTRGFNVVTTIDSADQQ VAYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSTATIEGD NLRFASGALSTNAQPNRRIRPGAIVRVVKNDAGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNNIGTKYA QQYITRFGFDADRHPAYLPMALGAGQVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVARAQPLVAEQNAPRAIDA RNAYVMNSLLQSVAQRGTGARTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVW IEYMGAALKGVPEFKPTVPDGVESLGGELYFTEFTPGHGFVSTVGVPQAPAAQNVDEAVPHVDEQEKQDIMNLFRGH >Mature_796_residues PMQSTTPTSPPPAPEPKKRPWWQKVLLGFAAMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVLI GEFGEERRDVVHFKDVPDSLKKAILAIEDARFYDHGGVDLTGIIRAGFVALTNGHASQGASTITMQVARNFFLSSEKTYT RKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRA KVRQEYILQRMLELNFITREQYDEAAAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYREETYTRGFNVVTTIDSADQQV AYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSTATIEGDN LRFASGALSTNAQPNRRIRPGAIVRVVKNDAGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQP GSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNNIGTKYAQ QYITRFGFDADRHPAYLPMALGAGQVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVARAQPLVAEQNAPRAIDAR NAYVMNSLLQSVAQRGTGARTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVWI EYMGAALKGVPEFKPTVPDGVESLGGELYFTEFTPGHGFVSTVGVPQAPAAQNVDEAVPHVDEQEKQDIMNLFRGH
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=796, Percent_Identity=38.8190954773869, Blast_Score=531, Evalue=1e-151, Organism=Escherichia coli, GI1786343, Length=249, Percent_Identity=38.5542168674699, Blast_Score=150, Evalue=2e-37, Organism=Escherichia coli, GI1788867, Length=229, Percent_Identity=31.8777292576419, Blast_Score=116, Evalue=7e-27, Organism=Escherichia coli, GI1789601, Length=178, Percent_Identity=32.5842696629214, Blast_Score=90, Evalue=5e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 87360; Mature: 87229
Theoretical pI: Translated: 7.25; Mature: 7.25
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPMQSTTPTSPPPAPEPKKRPWWQKVLLGFAAMCVALVVAGGLVLGYALVVAWPNMPSLD CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH ALTDYRPKVPLRIYTSDHVLIGEFGEERRDVVHFKDVPDSLKKAILAIEDARFYDHGGVD HHHCCCCCCCEEEECCCEEEEECCCCCHHCEEEECCCCHHHHHHHHHHHCCCEECCCCCC LTGIIRAGFVALTNGHASQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKD HHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH QILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR HHHHHHHHHHHHCCHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH AKVRQEYILQRMLELNFITREQYDEAAAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYR HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHH EETYTRGFNVVTTIDSADQQVAYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAID HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHH DALLEHPDNGELIAAVVTAASPRQITVAFIDGSTATIEGDNLRFASGALSTNAQPNRRIR HHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCEEEEECCCEEEECCCCCCCCCCCCCCC PGAIVRVVKNDAGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ CCCEEEEEECCCCCEEECCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHC PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTAL CCCCCCCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH QRSRNLVSIRILNNIGTKYAQQYITRFGFDADRHPAYLPMALGAGQVTPLQMAGAYSVFA HHCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHCCCEEEEE NGGYRVNPYLIAEVTDPNGAIVARAQPLVAEQNAPRAIDARNAYVMNSLLQSVAQRGTGA CCCEEECCEEEEEEECCCCEEEEECCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC RTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVW HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHH IEYMGAALKGVPEFKPTVPDGVESLGGELYFTEFTPGHGFVSTVGVPQAPAAQNVDEAVP HHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCEEECCCCCCCCCCCHHHHCC HVDEQEKQDIMNLFRGH CCCHHHHHHHHHHHCCC >Mature Secondary Structure PMQSTTPTSPPPAPEPKKRPWWQKVLLGFAAMCVALVVAGGLVLGYALVVAWPNMPSLD CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH ALTDYRPKVPLRIYTSDHVLIGEFGEERRDVVHFKDVPDSLKKAILAIEDARFYDHGGVD HHHCCCCCCCEEEECCCEEEEECCCCCHHCEEEECCCCHHHHHHHHHHHCCCEECCCCCC LTGIIRAGFVALTNGHASQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKD HHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH QILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR HHHHHHHHHHHHCCHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH AKVRQEYILQRMLELNFITREQYDEAAAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYR HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHH EETYTRGFNVVTTIDSADQQVAYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAID HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHH DALLEHPDNGELIAAVVTAASPRQITVAFIDGSTATIEGDNLRFASGALSTNAQPNRRIR HHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCEEEEECCCEEEECCCCCCCCCCCCCCC PGAIVRVVKNDAGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ CCCEEEEEECCCCCEEECCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHC PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTAL CCCCCCCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH QRSRNLVSIRILNNIGTKYAQQYITRFGFDADRHPAYLPMALGAGQVTPLQMAGAYSVFA HHCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHCCCEEEEE NGGYRVNPYLIAEVTDPNGAIVARAQPLVAEQNAPRAIDARNAYVMNSLLQSVAQRGTGA CCCEEECCEEEEEEECCCCEEEEECCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC RTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVW HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHH IEYMGAALKGVPEFKPTVPDGVESLGGELYFTEFTPGHGFVSTVGVPQAPAAQNVDEAVP HHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCEEECCCCCCCCCCCHHHHCC HVDEQEKQDIMNLFRGH CCCHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA