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The map label for this gene is yfjR [H]

Identifier: 116688331

GI number: 116688331

Start: 347886

End: 348755

Strand: Direct

Name: yfjR [H]

Synonym: Bcen2424_0307

Alternate gene names: 116688331

Gene position: 347886-348755 (Clockwise)

Preceding gene: 116688329

Following gene: 116688332

Centisome position: 9.99

GC content: 73.22

Gene sequence:

>870_bases
ATGGATCTCGGGTTTATCGGGCTGGGCGAAATGGGGCAGGCGATCGCGACGAACCTGCTGAAGGCGGGCCACACGGTGCG
GGTCTGGAACCGGTCGCGCGAGCGGGCCGAGCCGCTCGCGGCATTCGGCGCGCAGATCGTCGACACGCCGGCCGACGCGT
TTCGCGGCGACGCCGTGTTCTCGATGCTCGGCGACGATGCGGCCGCGCGCGCGGTGTTCGACGACGCGCTGCTGGCCCAG
GCGCCGCGCGGGCTGATCCACGTGAACATGGCGACGGTGTCGGTCGCGCTCGCCGAATCGCTCGCGCACGCGCATGCGTC
GCGCGGCATCGACTACGTCGCCGCGCCGGTGATGGGGCGGCCCGACGTCGCGGCCGCCGCGCGCCTGACGATCATGGCGG
GCGGCCCGGCCGAGGCGATCGACCGCGTGCAGCCGCTGTTCGACGCGATCGGCCAGAAGACCTGGCGCTTCGGCTCGCTG
CCGCAGCACGCGAACGTCGCGAAGATCGCCGCGAACTTCACGCTCGCGTCGGCGATCGAGACGCTCGGCGAGGCGTCCGC
GCTGCTCGGCGCGCACGGCGTCGCGATGCGCGATTTCCTCGACGTGATCACCAGCAGCGTGTTTCCGGGGCCCGTGTACG
AAGGCTACGGTGCGATGATCGCCGAGCGGCGCTACGAGCCCGCACGCTTCAAGGCGCGGCTCGGGCTGAAGGACGTCCGG
CTCGCGCTGGAGGCCGGCGACGCGACGTCGGTGCCGCTGCCGGTGGCGAGCGTCGTGCGCGACAGCCTGCTGGACGCGCT
CGCGCACGGCGGCGGCGACCAGGATTTCGCAGTGCTCGGCGAGGTCGCGCTGCGCCGCGCCGGCCGCTGA

Upstream 100 bases:

>100_bases
CCATCCGTGCAGTATCGCACCGCGCCCGGCCGGCGGCAGGCGCTGCAGCGCGCCGGCATCTGTGTTTAACTCGCCGCTTC
ACGGAGCGAAGGAGCGGGAC

Downstream 100 bases:

>100_bases
CACGCGACAGAAGCGCGACCCGCACGGCATAATCGGCGGTTCGTTTCCCCCTACATTTGAGGCAGAGGCAGTCATGAACT
TGCATACGTGGTGGCTTTTC

Product: 6-phosphogluconate dehydrogenase, NAD-binding

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRERAEPLAAFGAQIVDTPADAFRGDAVFSMLGDDAAARAVFDDALLAQ
APRGLIHVNMATVSVALAESLAHAHASRGIDYVAAPVMGRPDVAAAARLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGSL
PQHANVAKIAANFTLASAIETLGEASALLGAHGVAMRDFLDVITSSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
LALEAGDATSVPLPVASVVRDSLLDALAHGGGDQDFAVLGEVALRRAGR

Sequences:

>Translated_289_residues
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRERAEPLAAFGAQIVDTPADAFRGDAVFSMLGDDAAARAVFDDALLAQ
APRGLIHVNMATVSVALAESLAHAHASRGIDYVAAPVMGRPDVAAAARLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGSL
PQHANVAKIAANFTLASAIETLGEASALLGAHGVAMRDFLDVITSSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
LALEAGDATSVPLPVASVVRDSLLDALAHGGGDQDFAVLGEVALRRAGR
>Mature_289_residues
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRERAEPLAAFGAQIVDTPADAFRGDAVFSMLGDDAAARAVFDDALLAQ
APRGLIHVNMATVSVALAESLAHAHASRGIDYVAAPVMGRPDVAAAARLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGSL
PQHANVAKIAANFTLASAIETLGEASALLGAHGVAMRDFLDVITSSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
LALEAGDATSVPLPVASVVRDSLLDALAHGGGDQDFAVLGEVALRRAGR

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=282, Percent_Identity=30.1418439716312, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI23308751, Length=287, Percent_Identity=25.4355400696864, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=29.8245614035088, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=27.6595744680851, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790315, Length=300, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789092, Length=278, Percent_Identity=26.978417266187, Blast_Score=70, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24655230, Length=288, Percent_Identity=30.5555555555556, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19922568, Length=288, Percent_Identity=30.5555555555556, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI28574115, Length=267, Percent_Identity=26.5917602996255, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 30217; Mature: 30217

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRERAEPLAAFGAQIVDTPADAFRGDAVF
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCHHHHCCCHHHHHHHHHH
SMLGDDAAARAVFDDALLAQAPRGLIHVNMATVSVALAESLAHAHASRGIDYVAAPVMGR
HHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
PDVAAAARLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGSLPQHANVAKIAANFTLASAIE
CCHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
TLGEASALLGAHGVAMRDFLDVITSSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHH
LALEAGDATSVPLPVASVVRDSLLDALAHGGGDQDFAVLGEVALRRAGR
HEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLGFIGLGEMGQAIATNLLKAGHTVRVWNRSRERAEPLAAFGAQIVDTPADAFRGDAVF
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCHHHHCCCHHHHHHHHHH
SMLGDDAAARAVFDDALLAQAPRGLIHVNMATVSVALAESLAHAHASRGIDYVAAPVMGR
HHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
PDVAAAARLTIMAGGPAEAIDRVQPLFDAIGQKTWRFGSLPQHANVAKIAANFTLASAIE
CCHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
TLGEASALLGAHGVAMRDFLDVITSSVFPGPVYEGYGAMIAERRYEPARFKARLGLKDVR
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHCCHHHHH
LALEAGDATSVPLPVASVVRDSLLDALAHGGGDQDFAVLGEVALRRAGR
HEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]