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The map label for this gene is nolF [H]

Identifier: 116688328

GI number: 116688328

Start: 345133

End: 346338

Strand: Reverse

Name: nolF [H]

Synonym: Bcen2424_0304

Alternate gene names: 116688328

Gene position: 346338-345133 (Counterclockwise)

Preceding gene: 116688330

Following gene: 116688327

Centisome position: 9.94

GC content: 71.06

Gene sequence:

>1206_bases
TTGCCCGACCACAATCTGGACAAACTGAAAATCGACCGGCGTCCGATCGCACCGGTGCCGCGTCGGCGCCGGTGGGCCCG
CTACGCGGTCGCCGCCGCGCTGATCGTCATCGCGATCGGCGCCGGCCTCGCCCTCACCGGCCGGCCGACCGTCGACACCA
CCTCCGTCACGTCCGCCTACCCGTACCAGAACGACACGCAACTCAACGCGACCGGCTACGTCGTGCCGCAGCGCAAGGCC
GCGGTCGCGTCGAAGGGCCAGGGGCGCGTCGAATGGCTCGGCGTGCTCGAAGGCACGCGCGTGAAGAAGGACGAGATCAT
CGCGCGCCTCGAAAGCAACGACGTGCAGGCGTCGCTTGCGCAGGCGCGCGCGCAGGTGCAGGTCTCCCGCGCGAACCTCG
GCGTCGCGCAGGCCGAGCTGAAGGACGCCGAGATCGCATTGCGCCGCACGTCCACGCTCGCGCCGAAGGGCGCGGTGCCG
GCCGCGCAGCTCGATATCGACACGGCGCGCGTGAACAAGGCGCGCGCGACGCTCGACAGCGACCAGGCCGCGATCGCGTC
GGCCGAAGCGAACGCGCAGGCCGCGCAGGTGGCGGTCGACCAGACGGTGATCCGCGCGCCGTTCGACGGCATCGTGCTCG
CCAAGCATGCGAACGTCGGCGACAACATCACGCCGTTCTCGTCCGCGTCGGACAGCAAGGGCGCGGTCGTGACGATCGCC
GACATGGAGACGCTCGAGGTCGAGGCCGACGTCGCCGAATCGAACATCGCGAAGATCCGCGCCGAGCAGCCGTGCGAGAT
CCAGCTCGATGCGCTGCCCGACCTGCGCTTCGCGGGCCGCGTGTCGCGCATCGTGCCGACCGTCGACCGTTCGAAGGCCA
CCGTGCTCGTGAAGGTGCGCTTCGTCGACCGCGACGAACGCGTGCTGCCCGACATGAGCGCGAAGATCGCGTTCCTGTCG
AAGCCCGCGTCGGCGCAGGACCGGCAGCCGGTGACCGCCGTGCAGGCGAGCGCCGTGGTCGAGCGCGACGGCCGGCCGGT
CGTGTTCGTCGTGAAGGACGACGCCGTGCATGCGGTGGCCGTGGCGAAGGGCGCGCGGATCGGCGAACTCGTCGCGGTGC
GCGGCGTGACACCCGGCGACACGGTCGTGCTCGCGCCGGGCGCGAAGCTGAAGGACGGCGCGAACGTGACCGTCGCGAAG
AAGTAG

Upstream 100 bases:

>100_bases
GCGGCGCCGGGGCGACGCTTATACTGGCGTCCATCCGTCCCTTTCCCGGAAACCGGATCGCGACCGGTCACACGACCGCC
GCCGTCTCGAGGCGCTCAAA

Downstream 100 bases:

>100_bases
CGCGCGTGAACGACGCCCCGACGCCCCTCGTCGAGCTCAGCCACGTCGCGAAGTCGTACCGGCGCGGCAACCAGATCGTG
CCCGTGCTGACCGACATCTC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MPDHNLDKLKIDRRPIAPVPRRRRWARYAVAAALIVIAIGAGLALTGRPTVDTTSVTSAYPYQNDTQLNATGYVVPQRKA
AVASKGQGRVEWLGVLEGTRVKKDEIIARLESNDVQASLAQARAQVQVSRANLGVAQAELKDAEIALRRTSTLAPKGAVP
AAQLDIDTARVNKARATLDSDQAAIASAEANAQAAQVAVDQTVIRAPFDGIVLAKHANVGDNITPFSSASDSKGAVVTIA
DMETLEVEADVAESNIAKIRAEQPCEIQLDALPDLRFAGRVSRIVPTVDRSKATVLVKVRFVDRDERVLPDMSAKIAFLS
KPASAQDRQPVTAVQASAVVERDGRPVVFVVKDDAVHAVAVAKGARIGELVAVRGVTPGDTVVLAPGAKLKDGANVTVAK
K

Sequences:

>Translated_401_residues
MPDHNLDKLKIDRRPIAPVPRRRRWARYAVAAALIVIAIGAGLALTGRPTVDTTSVTSAYPYQNDTQLNATGYVVPQRKA
AVASKGQGRVEWLGVLEGTRVKKDEIIARLESNDVQASLAQARAQVQVSRANLGVAQAELKDAEIALRRTSTLAPKGAVP
AAQLDIDTARVNKARATLDSDQAAIASAEANAQAAQVAVDQTVIRAPFDGIVLAKHANVGDNITPFSSASDSKGAVVTIA
DMETLEVEADVAESNIAKIRAEQPCEIQLDALPDLRFAGRVSRIVPTVDRSKATVLVKVRFVDRDERVLPDMSAKIAFLS
KPASAQDRQPVTAVQASAVVERDGRPVVFVVKDDAVHAVAVAKGARIGELVAVRGVTPGDTVVLAPGAKLKDGANVTVAK
K
>Mature_400_residues
PDHNLDKLKIDRRPIAPVPRRRRWARYAVAAALIVIAIGAGLALTGRPTVDTTSVTSAYPYQNDTQLNATGYVVPQRKAA
VASKGQGRVEWLGVLEGTRVKKDEIIARLESNDVQASLAQARAQVQVSRANLGVAQAELKDAEIALRRTSTLAPKGAVPA
AQLDIDTARVNKARATLDSDQAAIASAEANAQAAQVAVDQTVIRAPFDGIVLAKHANVGDNITPFSSASDSKGAVVTIAD
METLEVEADVAESNIAKIRAEQPCEIQLDALPDLRFAGRVSRIVPTVDRSKATVLVKVRFVDRDERVLPDMSAKIAFLSK
PASAQDRQPVTAVQASAVVERDGRPVVFVVKDDAVHAVAVAKGARIGELVAVRGVTPGDTVVLAPGAKLKDGANVTVAKK

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=338, Percent_Identity=26.3313609467456, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081796, Length=378, Percent_Identity=26.1904761904762, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42416; Mature: 42285

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDHNLDKLKIDRRPIAPVPRRRRWARYAVAAALIVIAIGAGLALTGRPTVDTTSVTSAY
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEC
PYQNDTQLNATGYVVPQRKAAVASKGQGRVEWLGVLEGTRVKKDEIIARLESNDVQASLA
CCCCCCEECEEEEEECCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHEECCCCCHHHHHH
QARAQVQVSRANLGVAQAELKDAEIALRRTSTLAPKGAVPAAQLDIDTARVNKARATLDS
HHHHHEEEECCCCCEEHHHHCCHHEEEEHHCCCCCCCCCCCEEEECCHHHCCHHHHCCCC
DQAAIASAEANAQAAQVAVDQTVIRAPFDGIVLAKHANVGDNITPFSSASDSKGAVVTIA
CCHHEEECCCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
DMETLEVEADVAESNIAKIRAEQPCEIQLDALPDLRFAGRVSRIVPTVDRSKATVLVKVR
CCEEEEEECCHHHCCHHEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEEE
FVDRDERVLPDMSAKIAFLSKPASAQDRQPVTAVQASAVVERDGRPVVFVVKDDAVHAVA
EECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHEECCCCEEEEEEECCCEEEEE
VAKGARIGELVAVRGVTPGDTVVLAPGAKLKDGANVTVAKK
ECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
PDHNLDKLKIDRRPIAPVPRRRRWARYAVAAALIVIAIGAGLALTGRPTVDTTSVTSAY
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCEEC
PYQNDTQLNATGYVVPQRKAAVASKGQGRVEWLGVLEGTRVKKDEIIARLESNDVQASLA
CCCCCCEECEEEEEECCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHEECCCCCHHHHHH
QARAQVQVSRANLGVAQAELKDAEIALRRTSTLAPKGAVPAAQLDIDTARVNKARATLDS
HHHHHEEEECCCCCEEHHHHCCHHEEEEHHCCCCCCCCCCCEEEECCHHHCCHHHHCCCC
DQAAIASAEANAQAAQVAVDQTVIRAPFDGIVLAKHANVGDNITPFSSASDSKGAVVTIA
CCHHEEECCCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
DMETLEVEADVAESNIAKIRAEQPCEIQLDALPDLRFAGRVSRIVPTVDRSKATVLVKVR
CCEEEEEECCHHHCCHHEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEEE
FVDRDERVLPDMSAKIAFLSKPASAQDRQPVTAVQASAVVERDGRPVVFVVKDDAVHAVA
EECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHEECCCCEEEEEEECCCEEEEE
VAKGARIGELVAVRGVTPGDTVVLAPGAKLKDGANVTVAKK
ECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]