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The map label for this gene is yafC [C]

Identifier: 116688321

GI number: 116688321

Start: 338204

End: 339181

Strand: Reverse

Name: yafC [C]

Synonym: Bcen2424_0297

Alternate gene names: 116688321

Gene position: 339181-338204 (Counterclockwise)

Preceding gene: 116688325

Following gene: 116688320

Centisome position: 9.74

GC content: 72.7

Gene sequence:

>978_bases
ATGGCGAACGATGGCGCAGTAGCCGCTAAAATCGAAACCCGTCGTTTCTCCATGTGGACGCTGGAAATGAATCTGAACGA
TCTCTACCTGTTCGTGCAGGCGGTGGAGGCCGGCAGCATGTCGGCGGCCGCGCGCCGGCTCGACCTGCCGAAATCGACGG
TCAGCAAGCGCGTGGCCGAGCTGGAACGCACGCTCGGCGCGCGGCTCGTGCATCGCACGTCGCGCAGCTTCCGGCTCACG
CCGGTCGGCGCCGAATTCTTCGAGCATGCGCGCGCCGCCGTGATCGAGGCCGACAGCGCGCGCGACGCGGTGCTGCGGCA
GGCCGCCGAGCCGGCCGGCGTCGTGCGGATCACGAGCTCGGTGCCGGTCGCGCAGCACATCCTCGCGCCGTGCCTGCCGG
CGCTGGCGATCGCGCACCCGAAGCTGCTGTTGCAGATTCATGCGAGCGACCGCCTCGTCGACATCGCGCAGGAAGGCTTC
GACATCGCGGTGCGCAGCCATTTCGCGCCGCTGCCCGATTCCGCGCTCGTGCAACGACCGCTCGCGGCGTCGCCGATCGT
CGTCGTGGCCGCGCCGGCCTATCTCGCGCGGGCCGGCACGCCGGACACGCCGGCCGATCTCGCGCAGCACGACGGGCTCC
ACCCGGGCCCGCAGCCGTGGCGGCTGCAGCGCCGCGGCGATGCTGTCGACGTGACGCCGCGCCTGCGGCTGCAGGCCGAC
GAATCGACGCTGCTGCTACAGGCGGCGACGGCCGGCCTCGGCCTCGCGTGCCTGCCCGACTGGATCGCCGGCGCCGCGCT
CGCGTCCGGCGCGCTCGTGCGCGTGCTGCCCGGCTGGTGCGCCGGTACCGTCACGACGACGCTGCTGATGCCGCATCGGC
GCGGCCAGTTGCCCGGCGTGCGCGCCGCCGTCGAGTTTCTCGCGCAGCACGTGGCGCGCCATCACGGCAGCGGCAGCACG
ATCGAGCCGCACGCGTGA

Upstream 100 bases:

>100_bases
GCGCGACGATCGAGCCGAACGAGCAGCCGGCGGGAATACCGTAGAGAAGGATCGGGGACATCGGGAATTCCACAGAAGTT
GAACGAGCAGTCATGCTCCC

Downstream 100 bases:

>100_bases
CCACGTATCTCGCCGGATCGATTCAAATGCTGAATCGATCAAGTCAGCAATTGGCATGAAAGCGCAAATTTCGACATGAC
AGAATGGCCCGGACGCCCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MANDGAVAAKIETRRFSMWTLEMNLNDLYLFVQAVEAGSMSAAARRLDLPKSTVSKRVAELERTLGARLVHRTSRSFRLT
PVGAEFFEHARAAVIEADSARDAVLRQAAEPAGVVRITSSVPVAQHILAPCLPALAIAHPKLLLQIHASDRLVDIAQEGF
DIAVRSHFAPLPDSALVQRPLAASPIVVVAAPAYLARAGTPDTPADLAQHDGLHPGPQPWRLQRRGDAVDVTPRLRLQAD
ESTLLLQAATAGLGLACLPDWIAGAALASGALVRVLPGWCAGTVTTTLLMPHRRGQLPGVRAAVEFLAQHVARHHGSGST
IEPHA

Sequences:

>Translated_325_residues
MANDGAVAAKIETRRFSMWTLEMNLNDLYLFVQAVEAGSMSAAARRLDLPKSTVSKRVAELERTLGARLVHRTSRSFRLT
PVGAEFFEHARAAVIEADSARDAVLRQAAEPAGVVRITSSVPVAQHILAPCLPALAIAHPKLLLQIHASDRLVDIAQEGF
DIAVRSHFAPLPDSALVQRPLAASPIVVVAAPAYLARAGTPDTPADLAQHDGLHPGPQPWRLQRRGDAVDVTPRLRLQAD
ESTLLLQAATAGLGLACLPDWIAGAALASGALVRVLPGWCAGTVTTTLLMPHRRGQLPGVRAAVEFLAQHVARHHGSGST
IEPHA
>Mature_324_residues
ANDGAVAAKIETRRFSMWTLEMNLNDLYLFVQAVEAGSMSAAARRLDLPKSTVSKRVAELERTLGARLVHRTSRSFRLTP
VGAEFFEHARAAVIEADSARDAVLRQAAEPAGVVRITSSVPVAQHILAPCLPALAIAHPKLLLQIHASDRLVDIAQEGFD
IAVRSHFAPLPDSALVQRPLAASPIVVVAAPAYLARAGTPDTPADLAQHDGLHPGPQPWRLQRRGDAVDVTPRLRLQADE
STLLLQAATAGLGLACLPDWIAGAALASGALVRVLPGWCAGTVTTTLLMPHRRGQLPGVRAAVEFLAQHVARHHGSGSTI
EPHA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=30.8474576271186, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI145693193, Length=284, Percent_Identity=32.7464788732394, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789639, Length=259, Percent_Identity=30.1158301158301, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=29.0657439446367, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081978, Length=303, Percent_Identity=32.013201320132, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=27.3648648648649, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=29.9270072992701, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788706, Length=273, Percent_Identity=26.007326007326, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=27.9527559055118, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786448, Length=251, Percent_Identity=27.0916334661355, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=177, Percent_Identity=20.3389830508475, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=249, Percent_Identity=28.9156626506024, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=269, Percent_Identity=25.6505576208178, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34589; Mature: 34458

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDGAVAAKIETRRFSMWTLEMNLNDLYLFVQAVEAGSMSAAARRLDLPKSTVSKRVAE
CCCCCCEEEEEEEEEEEEEEEEECCCCEEEEEEHHHCCCHHHHHHHHCCCHHHHHHHHHH
LERTLGARLVHRTSRSFRLTPVGAEFFEHARAAVIEADSARDAVLRQAAEPAGVVRITSS
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHEEECCCHHHHHHHHHCCCCCEEEEECC
VPVAQHILAPCLPALAIAHPKLLLQIHASDRLVDIAQEGFDIAVRSHFAPLPDSALVQRP
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCEEEEHHCCCCCCCHHHHHCC
LAASPIVVVAAPAYLARAGTPDTPADLAQHDGLHPGPQPWRLQRRGDAVDVTPRLRLQAD
CCCCCEEEEECCHHHHCCCCCCCHHHHHHCCCCCCCCCCCEECCCCCEEECCCCEEEECC
ESTLLLQAATAGLGLACLPDWIAGAALASGALVRVLPGWCAGTVTTTLLMPHRRGQLPGV
CCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCCCCHH
RAAVEFLAQHVARHHGSGSTIEPHA
HHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ANDGAVAAKIETRRFSMWTLEMNLNDLYLFVQAVEAGSMSAAARRLDLPKSTVSKRVAE
CCCCCEEEEEEEEEEEEEEEEECCCCEEEEEEHHHCCCHHHHHHHHCCCHHHHHHHHHH
LERTLGARLVHRTSRSFRLTPVGAEFFEHARAAVIEADSARDAVLRQAAEPAGVVRITSS
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHEEECCCHHHHHHHHHCCCCCEEEEECC
VPVAQHILAPCLPALAIAHPKLLLQIHASDRLVDIAQEGFDIAVRSHFAPLPDSALVQRP
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCEEEEHHCCCCCCCHHHHHCC
LAASPIVVVAAPAYLARAGTPDTPADLAQHDGLHPGPQPWRLQRRGDAVDVTPRLRLQAD
CCCCCEEEEECCHHHHCCCCCCCHHHHHHCCCCCCCCCCCEECCCCCEEECCCCEEEECC
ESTLLLQAATAGLGLACLPDWIAGAALASGALVRVLPGWCAGTVTTTLLMPHRRGQLPGV
CCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCCCCHH
RAAVEFLAQHVARHHGSGSTIEPHA
HHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]