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The map label for this gene is gudD [H]

Identifier: 116688319

GI number: 116688319

Start: 335188

End: 336585

Strand: Reverse

Name: gudD [H]

Synonym: Bcen2424_0295

Alternate gene names: 116688319

Gene position: 336585-335188 (Counterclockwise)

Preceding gene: 116688320

Following gene: 116688318

Centisome position: 9.66

GC content: 70.96

Gene sequence:

>1398_bases
ATGTCCGAACCCAGCCGTGCCGGCACCCCGCGCGTCACGCGGATGCAGGTGATTCCCGTCGCCGGCCGCGACAGCATGCT
GCTCAACCTGTGCGGCGCGCACGCGCCGTATTTCACGCGCAATCTCGTGATCCTCGACGACAGCAGCGGGCATACGGGCG
TCGGCGAAGTGCCGGGCGGCGAAGGCATCCGCCACGCGCTCGAGCGCATGACGGATCTCGTCGTCGGCCAGTCGATCGGG
CGCTACCAGGCGACGCTCAACGCGGTGCGCGCGGCGCTGTCCGGCGCGGGCGCCGGCGCCGGCCGCACGATCCGCCACGA
AGTGACGTCGGCCGGCGAGGCGGCGGTGCTGCGCCAGCCGCACGAGATCAACCTGCGGCTCGACAACGTGATCACCGCGA
TCGAAGCCGCGCTGCTCGACCTGCTCGGCCAGCACCTCGACGTGCCGGTCGCGGCACTGCTCGGCGAAGGCCAGCAGCGC
GACGCGGTGCCGATGCTCGCGTATTTGTTCTACATCGGCGACCGGCGCCGCACCGATCTGCCGTATCGCGACGAGGCGCA
GGCGCGCGCCCCGTGGTTCCGGCTGCGCAACGAGGCGGCGCTCACGCCGGCCGCGATCGCGCGGCAGGCCGAAGCCGCGG
TCGAGCGCTACGGTTTCACCGATTTCAAGCTGAAGGGCGGCGTGATGGCCGGCGCCGACGAGATGGAAGCGATCGCGGCG
ATCAAGACGCGCTTTCCCGACGCGCGCGCGACGCTCGACCCGAACGGCGCATGGTCGCTCGACGAGGCCGTCGCGCTGTG
CCGCGGGCAAGGCCACCTGCTCGCGTATGCGGAAGATCCGTGCGGGCCGGAAGCCGGCTATTCGGGCCGCGAGGTGATGG
CCGAATTCCGCCGCGCGACGGGCATCCCGACCGCGACCAACATGATCGCGACCGACTGGCGGCAGATGGATCACGCGGTG
CGGCTGCAGGCGGTCGACATCCCGCTCGCCGATCCGCATTTCTGGACGATGCAGGGCTCGGTGCGGCTCGCGCAGCTGTG
CCGCGACTGGGGACTCACGTGGGGCTCGCACTCGAACAACCACTTCGACGTGTCGCTCGCGATGTTCACGCATGCGGCGG
CGGCCGCGCCCGGCACGATCACCGCGATCGACACGCACTGGATCTGGCAGGAAGGCGACGCGCGGCTCACGCGCGAACCG
CTCGCGATCGTCGGCGGCAAGGTGGCCGTGCCGGAACGGCCGGGGCTCGGCATCGAGCTGGACATGGCGCAGGTCGAGGC
CGCGCATGCGCTGTACAACGCGGTGGGTGGTACGGCGCGCGACGATGCGGTCGCGATGCGTTATCTGGTGCCGGGGTGGA
CGTATGATCCGAAGCGGCCGAGTTTGGGGCGGGTTTAG

Upstream 100 bases:

>100_bases
TCGTCGTCGGCGAAATTCGCCGCGTCGAGCTTCAATGAACGGCGCGGGCCGCGCCGGCGCACCCCGCGGCCCGCACGCCA
CCGGAACCAGGAGCGAACCG

Downstream 100 bases:

>100_bases
GGCAGAGTGGCGGGACGGTGGCGGTTGCCAAACGCAGGAAACGGTCGCATCCTGCCGGCTGCCTTGATCGGCCCGCGTGG
CGGACGGTCGGGCGCCCCCC

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSEPSRAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGGEGIRHALERMTDLVVGQSIG
RYQATLNAVRAALSGAGAGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQR
DAVPMLAYLFYIGDRRRTDLPYRDEAQARAPWFRLRNEAALTPAAIARQAEAAVERYGFTDFKLKGGVMAGADEMEAIAA
IKTRFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAV
RLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREP
LAIVGGKVAVPERPGLGIELDMAQVEAAHALYNAVGGTARDDAVAMRYLVPGWTYDPKRPSLGRV

Sequences:

>Translated_465_residues
MSEPSRAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGGEGIRHALERMTDLVVGQSIG
RYQATLNAVRAALSGAGAGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQR
DAVPMLAYLFYIGDRRRTDLPYRDEAQARAPWFRLRNEAALTPAAIARQAEAAVERYGFTDFKLKGGVMAGADEMEAIAA
IKTRFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAV
RLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREP
LAIVGGKVAVPERPGLGIELDMAQVEAAHALYNAVGGTARDDAVAMRYLVPGWTYDPKRPSLGRV
>Mature_464_residues
SEPSRAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGGEGIRHALERMTDLVVGQSIGR
YQATLNAVRAALSGAGAGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQRD
AVPMLAYLFYIGDRRRTDLPYRDEAQARAPWFRLRNEAALTPAAIARQAEAAVERYGFTDFKLKGGVMAGADEMEAIAAI
KTRFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAVR
LQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPL
AIVGGKVAVPERPGLGIELDMAQVEAAHALYNAVGGTARDDAVAMRYLVPGWTYDPKRPSLGRV

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=461, Percent_Identity=63.3405639913232, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1789151, Length=456, Percent_Identity=58.5526315789474, Blast_Score=536, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 50184; Mature: 50052

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPSRAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGG
CCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCHHHCEEEEEECCCCCCCCCCCCCC
EGIRHALERMTDLVVGQSIGRYQATLNAVRAALSGAGAGAGRTIRHEVTSAGEAAVLRQP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHCCC
HEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQRDAVPMLAYLFYIGDRRRTDL
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
PYRDEAQARAPWFRLRNEAALTPAAIARQAEAAVERYGFTDFKLKGGVMAGADEMEAIAA
CCCCCCHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHH
IKTRFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRAT
HHHCCCCCCCEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
GIPTATNMIATDWRQMDHAVRLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNN
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCC
HFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPLAIVGGKVAVPERPGLGIEL
EEEEEHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCHHHCCCEEECCCCCCCCEEE
DMAQVEAAHALYNAVGGTARDDAVAMRYLVPGWTYDPKRPSLGRV
HHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEPSRAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGHTGVGEVPGG
CCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCHHHCEEEEEECCCCCCCCCCCCCC
EGIRHALERMTDLVVGQSIGRYQATLNAVRAALSGAGAGAGRTIRHEVTSAGEAAVLRQP
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHCCC
HEINLRLDNVITAIEAALLDLLGQHLDVPVAALLGEGQQRDAVPMLAYLFYIGDRRRTDL
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
PYRDEAQARAPWFRLRNEAALTPAAIARQAEAAVERYGFTDFKLKGGVMAGADEMEAIAA
CCCCCCHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHH
IKTRFPDARATLDPNGAWSLDEAVALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRAT
HHHCCCCCCCEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
GIPTATNMIATDWRQMDHAVRLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNN
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCC
HFDVSLAMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPLAIVGGKVAVPERPGLGIEL
EEEEEHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCHHHCCCEEECCCCCCCCEEE
DMAQVEAAHALYNAVGGTARDDAVAMRYLVPGWTYDPKRPSLGRV
HHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]