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The map label for this gene is gidB [H]

Identifier: 116688118

GI number: 116688118

Start: 98405

End: 99091

Strand: Direct

Name: gidB [H]

Synonym: Bcen2424_0094

Alternate gene names: 116688118

Gene position: 98405-99091 (Clockwise)

Preceding gene: 116688117

Following gene: 116688119

Centisome position: 2.82

GC content: 63.76

Gene sequence:

>687_bases
ATGACGGCGCGTCGCGCGCCGGCGGTTAATCGGGACGTACTGGAAGGGATGCTCGTCGAAGGTACGGCGGCGCTCGACCT
CACGCTGACGGATACACAACGCAACCAGCTGCTCGACTATGTCGCGCTGCTCGGCAAGTGGAACGCGGTCTACAACCTGA
CCGCGATCCGCGACCCGATGCAGATGCTGATCCAGCACATCCTCGATTCGCTTTCCATCGTCCCGCATCTGCGCGGCCGT
ACATCGGCGCGCGTGCTCGACGTGGGCTCGGGCGGCGGGCTGCCCGGTATCGTGCTCGCGATCGTCGAGCCGGGCTGGCA
GGTCACGCTGAACGATATCGTGCAGAAGAAGTCTGCCTTCCAGACGCAGATGCGCGCAGAGCTGAAGCTCGCGAACCTGT
CGGTGGTCACCGGGCGGGTCGAATCGCTGCAGCCGGGTGTCGAAGTGCCGGAAAAATTCGACATGATCGTATCCCGCGCT
TTCGCGGATCTATCCGACTTCGTTAAACTTGCTCGACATCTGGTCGCGCCGGGCGGTTCGATCTGGGCGATGAAGGGCGT
TCACCCGGACGACGAGATTGCGCGGTTGCCGGAAGGCAGCCGTGTCACGCAGACGATCCGGCTGGCGGTGCCGATGCTCG
ATGCGGAGCGGCATCTGTTCGAAGTGGCCGTCGACGACGCGAATTGA

Upstream 100 bases:

>100_bases
CGCTGCTGATGGTGCACCTGAAGCGTCGTGGCCTGGGCCGCCGTAACGGTACGGCCGCAGAGGCCGCGGAGCAGGGGGAC
GGCGCCGTCCCGACGCAACA

Downstream 100 bases:

>100_bases
AGGCGCGCGGTAGCGCGCCGTTTGTTTGAAGGTAAAGGGAACACACCAACGATGGCAAAGATCTTCTGCGTTGCGAACCA
GAAGGGGGGCGTCGGCAAGA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTARRAPAVNRDVLEGMLVEGTAALDLTLTDTQRNQLLDYVALLGKWNAVYNLTAIRDPMQMLIQHILDSLSIVPHLRGR
TSARVLDVGSGGGLPGIVLAIVEPGWQVTLNDIVQKKSAFQTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRA
FADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVTQTIRLAVPMLDAERHLFEVAVDDAN

Sequences:

>Translated_228_residues
MTARRAPAVNRDVLEGMLVEGTAALDLTLTDTQRNQLLDYVALLGKWNAVYNLTAIRDPMQMLIQHILDSLSIVPHLRGR
TSARVLDVGSGGGLPGIVLAIVEPGWQVTLNDIVQKKSAFQTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRA
FADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVTQTIRLAVPMLDAERHLFEVAVDDAN
>Mature_227_residues
TARRAPAVNRDVLEGMLVEGTAALDLTLTDTQRNQLLDYVALLGKWNAVYNLTAIRDPMQMLIQHILDSLSIVPHLRGRT
SARVLDVGSGGGLPGIVLAIVEPGWQVTLNDIVQKKSAFQTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRAF
ADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVTQTIRLAVPMLDAERHLFEVAVDDAN

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=195, Percent_Identity=48.7179487179487, Blast_Score=181, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 24904; Mature: 24773

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARRAPAVNRDVLEGMLVEGTAALDLTLTDTQRNQLLDYVALLGKWNAVYNLTAIRDPM
CCCCCCCCCCHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCEEEHHHHHHHH
QMLIQHILDSLSIVPHLRGRTSARVLDVGSGGGLPGIVLAIVEPGWQVTLNDIVQKKSAF
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEECCCCEEEHHHHHHHHHHH
QTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRAFADLSDFVKLARHLVAPGGS
HHHHHHHHHHHHHHEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IWAMKGVHPDDEIARLPEGSRVTQTIRLAVPMLDAERHLFEVAVDDAN
EEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCC
>Mature Secondary Structure 
TARRAPAVNRDVLEGMLVEGTAALDLTLTDTQRNQLLDYVALLGKWNAVYNLTAIRDPM
CCCCCCCCCHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCEEEHHHHHHHH
QMLIQHILDSLSIVPHLRGRTSARVLDVGSGGGLPGIVLAIVEPGWQVTLNDIVQKKSAF
HHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEECCCCEEEHHHHHHHHHHH
QTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRAFADLSDFVKLARHLVAPGGS
HHHHHHHHHHHHHHEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IWAMKGVHPDDEIARLPEGSRVTQTIRLAVPMLDAERHLFEVAVDDAN
EEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA