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The map label for this gene is braD [H]

Identifier: 116688110

GI number: 116688110

Start: 87945

End: 88907

Strand: Direct

Name: braD [H]

Synonym: Bcen2424_0086

Alternate gene names: 116688110

Gene position: 87945-88907 (Clockwise)

Preceding gene: 116688109

Following gene: 116688111

Centisome position: 2.52

GC content: 65.21

Gene sequence:

>963_bases
ATGTGCCCGCTGCGCCGCCCGCGTGCGCGATGCAGGAACGTTGCGCACGCAACGAAACTCATTCTGACGGCTAAGTCGAT
GGACATCTTTGGCATTCCGTTGCCGGCGATGCTGAGCCAGTTGCTGCTCGGGCTCGTCAACGGCTCGTTCTACGCGATCC
TGAGCCTCGGGCTCGCGGTGATCTTCGGGCTGCTCAACGTGATCAACTTCGCGCACGGCGCGCTGTTCATGCTGGGTGCG
ATGCTCGCGTGGATGGGCCTGTCGTATTTCGGGCTGCCGTACTGGGCGATGCTCGTGCTCGCGCCCGTGCTCGTCGGCGC
GTTCGGGATCGCGATCGAGCGCTCGATGCTGCGCTGGCTGTACAAGCTCGATCACCTGTACGGGCTGCTGCTCACGTTCG
GCCTCACGCTGGTCGTCGAAGGCGTGTTCCGGTCGATCTACGGATCGTCGGGCCAGCCGTACGACGTGCCGTCGCAATTG
TCCGGCGCGACCAACCTCGGCTTCATGTTCCTGCCGAACTATCGCGCGTGGGTCGTCGTCGCATCGCTCGCGGTGTGCCT
CGCCACCTGGTTCGTGATCGAGAAGACACGGCTCGGCGCGTATCTGCGTGCCGGCACCGAGAACCCGAAGCTCGTCGAGG
CGTTCGGCGTGAACGTGCCGATGATGATCACGCTCACCTACGGCTTCGGCGTCGCGCTCGCCGCGTTCGCGGGCGTGCTG
GCCGCGCCGGTGATCCAGGTGTCGCCGCTGATGGGCCAGCCGATGATCATCACCGTGTTCGCGGTGGTCGTGATCGGCGG
GATGGGCTCGATCCTCGGCTCGATCGTCACGGGCCTGCTGCTCGGCGTGATCGAGGGCTTCACGCGCGTGTTCTATCCCG
AAGCGTCGGCCACCGTCGTGTTCGTGATCATGGCGATCGTGCTGCTGTTCCGCCCGGCGGGCCTCTTCGGCAAGGAAAAA
TGA

Upstream 100 bases:

>100_bases
ACCAGGCGTTCGGCACCAAGCAGGAAACGCGCTGCGCGCTGTGGAAGTAGGCATCGCGTAGCGGCGCGCACGGGCGCGTC
GCATGGTGGCGGCTGCAGCG

Downstream 100 bases:

>100_bases
TGCAGAGAAAAGTGCTCTACGGCGTGCTGCTCGCCGCACTGCTCGCCGCGCCGTTCATCGGCGCGTATCCGGTGTTCGTG
ATGAAGGTGCTCGCGTTCGC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MCPLRRPRARCRNVAHATKLILTAKSMDIFGIPLPAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGA
MLAWMGLSYFGLPYWAMLVLAPVLVGAFGIAIERSMLRWLYKLDHLYGLLLTFGLTLVVEGVFRSIYGSSGQPYDVPSQL
SGATNLGFMFLPNYRAWVVVASLAVCLATWFVIEKTRLGAYLRAGTENPKLVEAFGVNVPMMITLTYGFGVALAAFAGVL
AAPVIQVSPLMGQPMIITVFAVVVIGGMGSILGSIVTGLLLGVIEGFTRVFYPEASATVVFVIMAIVLLFRPAGLFGKEK

Sequences:

>Translated_320_residues
MCPLRRPRARCRNVAHATKLILTAKSMDIFGIPLPAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGA
MLAWMGLSYFGLPYWAMLVLAPVLVGAFGIAIERSMLRWLYKLDHLYGLLLTFGLTLVVEGVFRSIYGSSGQPYDVPSQL
SGATNLGFMFLPNYRAWVVVASLAVCLATWFVIEKTRLGAYLRAGTENPKLVEAFGVNVPMMITLTYGFGVALAAFAGVL
AAPVIQVSPLMGQPMIITVFAVVVIGGMGSILGSIVTGLLLGVIEGFTRVFYPEASATVVFVIMAIVLLFRPAGLFGKEK
>Mature_320_residues
MCPLRRPRARCRNVAHATKLILTAKSMDIFGIPLPAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGA
MLAWMGLSYFGLPYWAMLVLAPVLVGAFGIAIERSMLRWLYKLDHLYGLLLTFGLTLVVEGVFRSIYGSSGQPYDVPSQL
SGATNLGFMFLPNYRAWVVVASLAVCLATWFVIEKTRLGAYLRAGTENPKLVEAFGVNVPMMITLTYGFGVALAAFAGVL
AAPVIQVSPLMGQPMIITVFAVVVIGGMGSILGSIVTGLLLGVIEGFTRVFYPEASATVVFVIMAIVLLFRPAGLFGKEK

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=307, Percent_Identity=28.0130293159609, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 34394; Mature: 34394

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCPLRRPRARCRNVAHATKLILTAKSMDIFGIPLPAMLSQLLLGLVNGSFYAILSLGLAV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
IFGLLNVINFAHGALFMLGAMLAWMGLSYFGLPYWAMLVLAPVLVGAFGIAIERSMLRWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKLDHLYGLLLTFGLTLVVEGVFRSIYGSSGQPYDVPSQLSGATNLGFMFLPNYRAWVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEECCCCHHHHHH
ASLAVCLATWFVIEKTRLGAYLRAGTENPKLVEAFGVNVPMMITLTYGFGVALAAFAGVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHH
AAPVIQVSPLMGQPMIITVFAVVVIGGMGSILGSIVTGLLLGVIEGFTRVFYPEASATVV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FVIMAIVLLFRPAGLFGKEK
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MCPLRRPRARCRNVAHATKLILTAKSMDIFGIPLPAMLSQLLLGLVNGSFYAILSLGLAV
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
IFGLLNVINFAHGALFMLGAMLAWMGLSYFGLPYWAMLVLAPVLVGAFGIAIERSMLRWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKLDHLYGLLLTFGLTLVVEGVFRSIYGSSGQPYDVPSQLSGATNLGFMFLPNYRAWVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEECCCCHHHHHH
ASLAVCLATWFVIEKTRLGAYLRAGTENPKLVEAFGVNVPMMITLTYGFGVALAAFAGVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHH
AAPVIQVSPLMGQPMIITVFAVVVIGGMGSILGSIVTGLLLGVIEGFTRVFYPEASATVV
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FVIMAIVLLFRPAGLFGKEK
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]