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The map label for this gene is alkJ [H]

Identifier: 116688106

GI number: 116688106

Start: 82916

End: 84652

Strand: Direct

Name: alkJ [H]

Synonym: Bcen2424_0082

Alternate gene names: 116688106

Gene position: 82916-84652 (Clockwise)

Preceding gene: 116688103

Following gene: 116688107

Centisome position: 2.38

GC content: 68.34

Gene sequence:

>1737_bases
ATGACCCGAGTCAGTGCCAAAGCACTCACTCGGGTCGACAGGAGACACGCCGTGAGTACTCAACGTACGCTCGAGGGCGA
ATTCGACTACGTGATCGTCGGCGCGGGCACCGCAGGCTGCGTGCTCGCGAACCGCCTGACCGAGGATCCCGACATCAGCG
TGCTGCTGCTCGAAGCGGGCGGCAAGGACGACTACCACTGGATTCATATCCCGGTCGGCTATCTGTATTGCATCGGCAAC
CCGCGCACCGACTGGCTGTACAAGACGCAGCCTGAAGCGGCGCTCAACGGCCGCGCGCTGTCGTATCCGCGCGGCCGTGT
GCTCGGCGGCTGCTCGTCGATCAACGGAATGATCTACATGCGCGGCCAGCGCGAGGACTACGACAACTGGGCGCAGGAAA
CCGGCGACGCCGGCTGGTCGTGGGACAGCGTGCTGCCGATCTTCAAGCGCAGCGAGGATCATCATGCGGGCGCGAGCGAT
GCGCACGGCGCGGGCGGCTACTGGCGCGTCGAGAAGCAGCGGCTGCGCTGGGAAATCCTCGAATCGTTCGCGCAGGCCGC
GCAGCAGACGGGCATCCCCGCCACCGACGATTTCAACCGCGGCGACAATGCCGGGGTCGGCTACTTCGAAGTGAACCAGA
AGCGCGGCGTGCGCTGGAATACGTCGAAGGCGTTCCTGCGGCCCGCGATGGCGCGCCCGAACCTGACCGTGATCACCGGC
GCGCATGCGCAACGCGTGATTTTCGACGGGCGGCGCGCGGTGGGTGTCGAGTACCACGGCGGCGGCACCGACTACGTCGC
GCGGGCGCGCTCGGAAGTGCTGCTGACGTCGGGCGCGGTGAATTCGCCGCAACTGCTCGAACTGTCGGGTATCGGCGATG
GCCGGCGGCTGCAGGCGCTCGGCATCGACGTCGTGCAGGATCTGCCGGGCGTCGGCGAAAACCTGCAGGATCACCTGCAA
TTGCGGATGGCGTTTCGTGTCGACGGCGTGCGCACGCTGAACACGCTGTCCGCGCGCTGGTGGGGCAAGCTGATGATCGG
CGCCGAATATGCGCTGCTGCAGCGCGGGCCGATGTCGATGGCGCCATCGCAGTTGGGCGCGTTCGCGAAATCCGATCCGG
ACGATCCCGCACTCACGCGCCCCGATCTCCAGTACCACGTGCAGCCGCTGTCGCTCGAACGCTTCGGCGAGCCGTTGCAC
GGCTTCAACGCCTTTACGGCGTCGGTCTGCCATTTGCGGCCGAGCTCGCGCGGCAGCGTGCACGTCACGAGCGCCGATCC
GGCCAGCGCACCGGCGATCGCGCCGAACTATCTGTCGACCGACCACGATCGCCACGTCGCCGCGAACGCGCTGCGCCTCA
CGCGCCGGATCGCCGCCGCACCGGCGCTCGCACGCTATCGCCCCGAGGAAATCCTGCCCGGCCCGCAGTACCGGACCGAG
GCCGAACTGATCGACGCAGCCGGCGCCGTCGGCACGACGATCTTCCACCCGGTCGGCACGTGCCGGATGGGGCGCGCCGA
CGACGCGCGCGCGGTGGTCGACAGCCGGCTGCGCGTGCGCAGCATCGCCGGGTTGCGGATCGTCGATGCGTCGGTGATGC
CGTTCATCACGTCGGGCAACACCAATTCGCCGACGCTGATGATCGCCGAGCGCGCGAGCGACATGATTCGTGCCGACCGC
CGTGCGGCGCGCGAAGCGATGGCGGTGCGCACCGAAGCCGCGGTAACGGCGGCATGA

Upstream 100 bases:

>100_bases
GCTGAAAATGACGCGCGGTATCGCGCCGCGCGGTACACAACAACGCGCTCGCAGGGGCCGGAGTCAGCGCTGGAGCGCTC
GTCCGGGTCGACCGCGAAGC

Downstream 100 bases:

>100_bases
CGACCCGCTGACGCGCACGCCAGCGGGCGGTCATGCGAACGCCCGCTGATTCGCTATGCGAAACAAGCTGCCGTCGCATC
CGCTCGAACGGCAAGATCCG

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MTRVSAKALTRVDRRHAVSTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDISVLLLEAGGKDDYHWIHIPVGYLYCIGN
PRTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYMRGQREDYDNWAQETGDAGWSWDSVLPIFKRSEDHHAGASD
AHGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITG
AHAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGRRLQALGIDVVQDLPGVGENLQDHLQ
LRMAFRVDGVRTLNTLSARWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPALTRPDLQYHVQPLSLERFGEPLH
GFNAFTASVCHLRPSSRGSVHVTSADPASAPAIAPNYLSTDHDRHVAANALRLTRRIAAAPALARYRPEEILPGPQYRTE
AELIDAAGAVGTTIFHPVGTCRMGRADDARAVVDSRLRVRSIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADR
RAAREAMAVRTEAAVTAA

Sequences:

>Translated_578_residues
MTRVSAKALTRVDRRHAVSTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDISVLLLEAGGKDDYHWIHIPVGYLYCIGN
PRTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYMRGQREDYDNWAQETGDAGWSWDSVLPIFKRSEDHHAGASD
AHGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITG
AHAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGRRLQALGIDVVQDLPGVGENLQDHLQ
LRMAFRVDGVRTLNTLSARWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPALTRPDLQYHVQPLSLERFGEPLH
GFNAFTASVCHLRPSSRGSVHVTSADPASAPAIAPNYLSTDHDRHVAANALRLTRRIAAAPALARYRPEEILPGPQYRTE
AELIDAAGAVGTTIFHPVGTCRMGRADDARAVVDSRLRVRSIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADR
RAAREAMAVRTEAAVTAA
>Mature_577_residues
TRVSAKALTRVDRRHAVSTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDISVLLLEAGGKDDYHWIHIPVGYLYCIGNP
RTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYMRGQREDYDNWAQETGDAGWSWDSVLPIFKRSEDHHAGASDA
HGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITGA
HAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGRRLQALGIDVVQDLPGVGENLQDHLQL
RMAFRVDGVRTLNTLSARWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPALTRPDLQYHVQPLSLERFGEPLHG
FNAFTASVCHLRPSSRGSVHVTSADPASAPAIAPNYLSTDHDRHVAANALRLTRRIAAAPALARYRPEEILPGPQYRTEA
ELIDAAGAVGTTIFHPVGTCRMGRADDARAVVDSRLRVRSIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADRR
AAREAMAVRTEAAVTAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=42.0074349442379, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1786503, Length=545, Percent_Identity=40.5504587155963, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17532301, Length=587, Percent_Identity=34.412265758092, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI45551458, Length=596, Percent_Identity=36.5771812080537, Blast_Score=344, Evalue=1e-94,
Organism=Drosophila melanogaster, GI45549471, Length=596, Percent_Identity=36.5771812080537, Blast_Score=344, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24642055, Length=594, Percent_Identity=36.7003367003367, Blast_Score=343, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24642042, Length=575, Percent_Identity=36.3478260869565, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24642059, Length=568, Percent_Identity=36.443661971831, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24642048, Length=571, Percent_Identity=34.8511383537653, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24650267, Length=566, Percent_Identity=34.6289752650177, Blast_Score=285, Evalue=8e-77,
Organism=Drosophila melanogaster, GI18859995, Length=586, Percent_Identity=34.9829351535836, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI17137792, Length=557, Percent_Identity=34.4703770197487, Blast_Score=282, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24642035, Length=576, Percent_Identity=32.9861111111111, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24642039, Length=580, Percent_Identity=35, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI18859993, Length=576, Percent_Identity=31.7708333333333, Blast_Score=269, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24642037, Length=576, Percent_Identity=32.1180555555556, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24642051, Length=595, Percent_Identity=30.7563025210084, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24642057, Length=592, Percent_Identity=28.3783783783784, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24645930, Length=583, Percent_Identity=27.7873070325901, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 63054; Mature: 62923

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVSAKALTRVDRRHAVSTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDISVLLLEAG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECC
GKDDYHWIHIPVGYLYCIGNPRTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYM
CCCCEEEEEEEEEEEEECCCCCCCCEEECCCCHHHCCCEECCCCCCEEECCCCCCCEEEE
RGQREDYDNWAQETGDAGWSWDSVLPIFKRSEDHHAGASDAHGAGGYWRVEKQRLRWEIL
ECCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
ESFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITG
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEEC
AHAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGRRLQAL
CCCEEEEECCCEEEEEEEECCCCHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCEEEEE
GIDVVQDLPGVGENLQDHLQLRMAFRVDGVRTLNTLSARWWGKLMIGAEYALLQRGPMSM
CCHHHHHCCCCCCCHHHHHHEEHEEEECCCHHHHHHHHHHHHEEEECCHHHHHCCCCCCC
APSQLGAFAKSDPDDPALTRPDLQYHVQPLSLERFGEPLHGFNAFTASVCHLRPSSRGSV
CHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHCCCCCCCCHHHHHHEEEECCCCCCCE
HVTSADPASAPAIAPNYLSTDHDRHVAANALRLTRRIAAAPALARYRPEEILPGPQYRTE
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHCCCHHCCCCCCCCCH
AELIDAAGAVGTTIFHPVGTCRMGRADDARAVVDSRLRVRSIAGLRIVDASVMPFITSGN
HHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEECCC
TNSPTLMIAERASDMIRADRRAAREAMAVRTEAAVTAA
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
TRVSAKALTRVDRRHAVSTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDISVLLLEAG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECC
GKDDYHWIHIPVGYLYCIGNPRTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYM
CCCCEEEEEEEEEEEEECCCCCCCCEEECCCCHHHCCCEECCCCCCEEECCCCCCCEEEE
RGQREDYDNWAQETGDAGWSWDSVLPIFKRSEDHHAGASDAHGAGGYWRVEKQRLRWEIL
ECCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHH
ESFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITG
HHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEEC
AHAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGRRLQAL
CCCEEEEECCCEEEEEEEECCCCHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCEEEEE
GIDVVQDLPGVGENLQDHLQLRMAFRVDGVRTLNTLSARWWGKLMIGAEYALLQRGPMSM
CCHHHHHCCCCCCCHHHHHHEEHEEEECCCHHHHHHHHHHHHEEEECCHHHHHCCCCCCC
APSQLGAFAKSDPDDPALTRPDLQYHVQPLSLERFGEPLHGFNAFTASVCHLRPSSRGSV
CHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHCCCCCCCCHHHHHHEEEECCCCCCCE
HVTSADPASAPAIAPNYLSTDHDRHVAANALRLTRRIAAAPALARYRPEEILPGPQYRTE
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHCCCHHCCCCCCCCCH
AELIDAAGAVGTTIFHPVGTCRMGRADDARAVVDSRLRVRSIAGLRIVDASVMPFITSGN
HHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCEEECCC
TNSPTLMIAERASDMIRADRRAAREAMAVRTEAAVTAA
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]