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The map label for this gene is mnmC [H]

Identifier: 116688090

GI number: 116688090

Start: 67230

End: 69161

Strand: Direct

Name: mnmC [H]

Synonym: Bcen2424_0066

Alternate gene names: 116688090

Gene position: 67230-69161 (Clockwise)

Preceding gene: 116688088

Following gene: 116688091

Centisome position: 1.93

GC content: 72.31

Gene sequence:

>1932_bases
ATGCCCGACCGACTTGTTTCCGCCACGCTCGCGCTTCGCGACGACGGCACGCTCGTCTCGCCCGAGTTCGGCGAGCTTCA
TCGCGGTGCATCCGGCACGCTCGCTCGCGCGCATCGCACGTTCGTCGCGGGCAACGGGCTGCCCGCGCGCTGGCAGCGCC
GCCGAACGTTCACGATCGTCACGACCGCGTTCGGCGCGGGCGCCGGCTTCCTCGCGGCCTGGGCCGCGTGGCGCGACGAT
CCCGCGCGCTGCGAACGGCTGCACGTCGTCGCGGTCGAACCGCATCCGTTCTCGCGCGACGACCTGCACCGCGCGGTCTC
GCATATGGTTGTAGACACAACCATATCGGCAGACGTCGACGCGTTGCTCGATGCCTGGCCGATACTCGTGCCGGGCCTGC
ACCGGCTCGAATTCGACGAAGGGCGCGTGGTGCTCACGCTCGCGTTCGGCGATACGATCGACTTGTTGAAGAAGCTCGTC
GCGCGCGCCGATGCGTTTTTTCTCGACGGCGCCGCGGCATCGGGCGACGGGATTCGCGCACTCGCGAAACTCGCCGGCGA
ACACGCGACGTTTGCGACGCATGCAAAGTCCGACGACGTGAAACACGCGCTCGGCGAAACAGGTTTCACGTTCCGCGAAG
TCGACGATCGCCTCGTCGGCGACTATGCGCCGCGCTGGCGCGCGCGCCGGCACGAGCCGCCGCGTGCGCTGCCCGTTGCC
GCGCGCCGCGCGATCGTGATCGGCGCGGGTCTCGCCGGCTGCGCGGTGGTCGAACGGCTGGCCGCGCGCGGCTGGGACGT
CACGCTGATCGAACGGCACGAACGGATCGCGAGCGAAGCGTCGGGCAATCCGGCCGGTGTGTTCCATCCATTGATGACGC
GCGATGACAACGTCGCGAGCCGGCTCACGCGCGGCGGCTTCCTGCACGCGCTCGCACGCTGGCGTGCGCTCGAACGCGCG
GGGCATGCATTCTCACGCAGCACGCACGGGATGCTCCATCTCGCCGAATCGGCCGACGATTTCGCGCGGATGCGCGACGC
GTTCGACGCGTTCGGGCCGCCGTCCGACTATGCAACGCTGCTCGATGCCGATGCCGCGCGGGCGCACCTGAATCTGCCGG
TCGCGCAGGGCGGCCTGCTGTTTCCGCACGGCGGCGCCGTGTGGCCGGCCGGGCTGTGCGTCGCGCAGTATGCGGCGGCC
GGCGAGCGCGTGCGGCTGCTGGCGTCCACCTGCGTCGCACGGCTCGAACGGCGGGACGACACCTGGCATGCACTCGACGA
TACCGGCGCCACGCTGGCCGATGCGCCCGTCGTCGTGCTCGCGAACGCGGGCGACGCCGCGCGTCTTGCCGGGCTGCGGC
ACGTCACGCTGCAACCGGTGCGCGGCCAGCTCACGCTGTTGCCGCCGGGCACGACGGCGCCGCTGCCGTGCCCGGCGATC
GGCGACGGTTACGCGGTCCCGCTCGACGACGGCACGCTGCTGATCGGCGCCACCTTCGAACCGGACGACACCGATCCCGC
GATGCGCGCGGCCGGCCACGCCGAAAACCTCGATCGCGTGCGGCACCTGCTGCCGGGCCTGATCGGCGCGCTGCCGGATC
CCGCCACGCTGCGCGGGCGCGTCGCATTCCGCTGGGTCGTCGGCGACCGCCTGCCGCTCATCGGCCCGCTCGCCGACGAA
ACGCAGGCCACCGCGAACGCACGCGCGCTGGGCGGTGCGCAGGCGCGCGACCTGCCGCGCATGCCGGGCCTCTACGGCGC
CTTCGGCTTCGGTTCGCGCGGCCTCGTGTGGGCCGCGCTCGGCGCCGAACTCATCGCATCGCAACTCGAAGGCGAGCCCT
GGCCGCTCGAGCGCGAACTCGCCGACGCCGTCGATCCCGCCCGCTTCCTGATCCGCGCACTGCGCGCCCGCCGCGTCGGC
TCGGCGGGCTGA

Upstream 100 bases:

>100_bases
TGGTCCGCCCGATCGTCGCGCCGTTGCCCCTTTCCAACGGCCGGTTGGACGGCCTCCCGCAATCCCTTGCCAGGCTTGGC
TGCCACGTTTTTTGTTTCGC

Downstream 100 bases:

>100_bases
CCTCGCGCGCGCCCCCTCACAGTTTCCCGATTTTTCCGCGCAAGGTTATCCACGCGATGCTGTGGATAACCGCGCGGAAT
CCGCGCGCACTTCGTGTGCA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MPDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIVTTAFGAGAGFLAAWAAWRDD
PARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVDALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLV
ARADAFFLDGAAASGDGIRALAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVA
ARRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRGGFLHALARWRALERA
GHAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATLLDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAA
GERVRLLASTCVARLERRDDTWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAI
GDGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGRVAFRWVVGDRLPLIGPLADE
TQATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVG
SAG

Sequences:

>Translated_643_residues
MPDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIVTTAFGAGAGFLAAWAAWRDD
PARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVDALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLV
ARADAFFLDGAAASGDGIRALAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVA
ARRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRGGFLHALARWRALERA
GHAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATLLDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAA
GERVRLLASTCVARLERRDDTWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAI
GDGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGRVAFRWVVGDRLPLIGPLADE
TQATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVG
SAG
>Mature_642_residues
PDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIVTTAFGAGAGFLAAWAAWRDDP
ARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVDALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLVA
RADAFFLDGAAASGDGIRALAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVAA
RRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVASRLTRGGFLHALARWRALERAG
HAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATLLDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAAG
ERVRLLASTCVARLERRDDTWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAIG
DGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGRVAFRWVVGDRLPLIGPLADET
QATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVGS
AG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=680, Percent_Identity=27.9411764705882, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 68644; Mature: 68512

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00646 RIBOSOMAL_S13_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIV
CCCCCEEEEEEEECCCCEECCCHHHHHCCCCCCHHHHHHHEEECCCCCHHHHCCEEEEEE
TTAFGAGAGFLAAWAAWRDDPARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVD
EECCCCCHHHHHHHHHCCCCHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHH
ALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLVARADAFFLDGAAASGDGIRA
HHHHHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCEEECCCCCCCHHHHH
LAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVA
HHHHHCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCHH
ARRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVAS
HCCEEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCEEECHHHCCCCHHHH
RLTRGGFLHALARWRALERAGHAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATL
HHHCCHHHHHHHHHHHHHHHCHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHH
LDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAAGERVRLLASTCVARLERRDD
HCCCHHHHEECCEEECCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
TWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAI
CCEECCCCCCEECCCCEEEEECCCCHHHHHCCCEEEEECCCCEEEEECCCCCCCCCCCCC
GDGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGR
CCCEEEECCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
VAFRWVVGDRLPLIGPLADETQATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAAL
EEEEEEECCCCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCCCCEECCCCCCCHHHHHH
GAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVGSAG
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PDRLVSATLALRDDGTLVSPEFGELHRGASGTLARAHRTFVAGNGLPARWQRRRTFTIV
CCCCEEEEEEEECCCCEECCCHHHHHCCCCCCHHHHHHHEEECCCCCHHHHCCEEEEEE
TTAFGAGAGFLAAWAAWRDDPARCERLHVVAVEPHPFSRDDLHRAVSHMVVDTTISADVD
EECCCCCHHHHHHHHHCCCCHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHH
ALLDAWPILVPGLHRLEFDEGRVVLTLAFGDTIDLLKKLVARADAFFLDGAAASGDGIRA
HHHHHHHHHCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCEEECCCCCCCHHHHH
LAKLAGEHATFATHAKSDDVKHALGETGFTFREVDDRLVGDYAPRWRARRHEPPRALPVA
HHHHHCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCHH
ARRAIVIGAGLAGCAVVERLAARGWDVTLIERHERIASEASGNPAGVFHPLMTRDDNVAS
HCCEEEEECCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCEEECHHHCCCCHHHH
RLTRGGFLHALARWRALERAGHAFSRSTHGMLHLAESADDFARMRDAFDAFGPPSDYATL
HHHCCHHHHHHHHHHHHHHHCHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHH
LDADAARAHLNLPVAQGGLLFPHGGAVWPAGLCVAQYAAAGERVRLLASTCVARLERRDD
HCCCHHHHEECCEEECCCEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
TWHALDDTGATLADAPVVVLANAGDAARLAGLRHVTLQPVRGQLTLLPPGTTAPLPCPAI
CCEECCCCCCEECCCCEEEEECCCCHHHHHCCCEEEEECCCCEEEEECCCCCCCCCCCCC
GDGYAVPLDDGTLLIGATFEPDDTDPAMRAAGHAENLDRVRHLLPGLIGALPDPATLRGR
CCCEEEECCCCEEEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
VAFRWVVGDRLPLIGPLADETQATANARALGGAQARDLPRMPGLYGAFGFGSRGLVWAAL
EEEEEEECCCCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCCCCEECCCCCCCHHHHHH
GAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVGSAG
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA