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The map label for this gene is outE [H]

Identifier: 116688085

GI number: 116688085

Start: 60284

End: 61783

Strand: Reverse

Name: outE [H]

Synonym: Bcen2424_0061

Alternate gene names: 116688085

Gene position: 61783-60284 (Counterclockwise)

Preceding gene: 116688086

Following gene: 116688084

Centisome position: 1.77

GC content: 68.67

Gene sequence:

>1500_bases
GTGAGCGACGTGCTCGCGTCCTCCGCCCACGACGGCGCGCCGGGCGAACGCCAGCCGCCGTCGCCGCTCGCCGCGCGCCT
GCTGCCGTACGGCTTCGCGAAGAGCGGCCAGGTGCTGATCGCGCATCAGCTCGACGACACGCTCGAGGTATGGATCAGCG
AACGCACGAGCGACGCCGCGCTCGCGGAGATCGCGCGCAACTTCGGCTCGATCGCCGTGCATCGCGTGCCGGCCGACGAG
CTCGCACAGGCGATCAACCAGGCCTACGCACGCCAGGACGGCAGCGCCGCGCAGGTGGTCGGCGAGGTCGAAGGCGAAGT
CGACCTGTCGCGGCTGATGCAGGACATTCCCGAAGTCGAGGATCTGCTCGAATCGGAAGACGATGCGCCGATCATCCGGA
TGATCAACGCGCTGCTCACGCAAGCGGCGCGCGAACAGGCATCGGACATTCACATCGAGCCATTCGAGAATGCGTCGGTC
GTGCGCTTTCGCGTCGACGGCACGCTGCGCGACGTCGTGCGCCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGGAT
CAAGATCATGGCGCAGCTCGACATCGCGGAGAAGCGTCTGCCGCAGGACGGCCGCATCACGCTGCGCGTCGGCGGCCGGC
CGGTCGACGTGCGGGTGTCGACGCTGCCGACCGGGCACGGCGAGCGCGCGGTGCTGCGTCTGCTCGAAAAGGATGCGCAG
CGCCTGAACCTCGAAGCGCTCGGGATGGGCCGCGACACGCTCGTCCAGTTCGACAAGCTGATCGGCCGCCCGCACGGCAT
CGTGCTCGTCACCGGCCCGACCGGTTCGGGCAAGACGACCACGCTGTACGCGTCGATGTCGCGGCTCGAAACCGCGACGA
CCAACATCATGACCGTCGAGGACCCGATCGAATACGACCTGTCCGGCATTGGCCAGACGCAGGTGAACGAGCGAATCGGG
ATGACCTTTGCCCGCGCGCTGCGCTCGATTCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAAATCCGCGACCTCGA
AACCGCGCAGATCGCGGTGCAGGCGTCGCTGACGGGCCACCTCGTGCTCGCGACGCTGCACACGAACGACGCGGCGTCCG
CCGTCACGCGCCTGACCGACATGGGCGTCGAGCCGTACCTGCTCGCGTCGTCGCTGCTCGGCGTGCTCGCGCAGCGCCTG
GTGCGCCAGCTCTGCCCGGTCTGCAAGGAAGAGCGGCATGAGGACGGCCGTACCGTATGGCATCCGGTCGGTTGCGCGAA
GTGCGGACATTCCGGTTATGCGGGCCGGCGCGGTGTGTACGAGCTGCTGGTGATCGACGATTCGATCCGCTCGCTGATTC
ACCGCAACGCGGCCGATGCTGAAATTCTCGCCGCCGGCCGCGCGGAAGGCATGCGCACGCTGCGCGACGACGCCGAGCGC
TGGCTCGCGTCCGGCGCGACGTCGCTCGAGGAAGTGCTGCGCGTGACGGGAGGCGCATAG

Upstream 100 bases:

>100_bases
CTGCCCAGCCATTGCCGGAAGCAACGCCCGCGCAGCCGCAAAGCGTGCCGCAGCAGGCGGTGCCGCAACAGCCGCTGACC
ACCGCGCCGGGAGCCTCGCA

Downstream 100 bases:

>100_bases
CGCGATGCCGGCATTCCGTTTCGAAGCAATCGATGCGGCGGGACGCGCGCAGAAAGGCGTGATCGATGCCGACAGTGCGC
GCGCCGCGCGCGGCCAGCTG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSDVLASSAHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAALAEIARNFGSIAVHRVPADE
LAQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASV
VRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQ
RLNLEALGMGRDTLVQFDKLIGRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIG
MTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRL
VRQLCPVCKEERHEDGRTVWHPVGCAKCGHSGYAGRRGVYELLVIDDSIRSLIHRNAADAEILAAGRAEGMRTLRDDAER
WLASGATSLEEVLRVTGGA

Sequences:

>Translated_499_residues
MSDVLASSAHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAALAEIARNFGSIAVHRVPADE
LAQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASV
VRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQ
RLNLEALGMGRDTLVQFDKLIGRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIG
MTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRL
VRQLCPVCKEERHEDGRTVWHPVGCAKCGHSGYAGRRGVYELLVIDDSIRSLIHRNAADAEILAAGRAEGMRTLRDDAER
WLASGATSLEEVLRVTGGA
>Mature_498_residues
SDVLASSAHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAALAEIARNFGSIAVHRVPADEL
AQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVV
RFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQR
LNLEALGMGRDTLVQFDKLIGRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGM
TFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLV
RQLCPVCKEERHEDGRTVWHPVGCAKCGHSGYAGRRGVYELLVIDDSIRSLIHRNAADAEILAAGRAEGMRTLRDDAERW
LASGATSLEEVLRVTGGA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=420, Percent_Identity=54.2857142857143, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1786296, Length=439, Percent_Identity=42.8246013667426, Blast_Score=334, Evalue=9e-93,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=36.1702127659575, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54102; Mature: 53971

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVLASSAHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHH
LAEIARNFGSIAVHRVPADELAQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVE
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCHHHH
DLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALH
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
GALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQ
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHH
RLNLEALGMGRDTLVQFDKLIGRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVE
HCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEEC
DPIEYDLSGIGQTQVNERIGMTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGH
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCE
LVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEERHEDGRTVW
EEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HPVGCAKCGHSGYAGRRGVYELLVIDDSIRSLIHRNAADAEILAAGRAEGMRTLRDDAER
CCCCHHHCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
WLASGATSLEEVLRVTGGA
HHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SDVLASSAHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAA
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHH
LAEIARNFGSIAVHRVPADELAQAINQAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVE
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCHHHH
DLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALH
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
GALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQ
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHH
RLNLEALGMGRDTLVQFDKLIGRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVE
HCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEEC
DPIEYDLSGIGQTQVNERIGMTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGH
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCE
LVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEERHEDGRTVW
EEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HPVGCAKCGHSGYAGRRGVYELLVIDDSIRSLIHRNAADAEILAAGRAEGMRTLRDDAER
CCCCHHHCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
WLASGATSLEEVLRVTGGA
HHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]