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The map label for this gene is rhmD [H]

Identifier: 116626755

GI number: 116626755

Start: 9701971

End: 9703215

Strand: Direct

Name: rhmD [H]

Synonym: Acid_7728

Alternate gene names: 116626755

Gene position: 9701971-9703215 (Clockwise)

Preceding gene: 116626754

Following gene: 116626756

Centisome position: 97.35

GC content: 63.13

Gene sequence:

>1245_bases
ATGAAGATCACGAGCGTTCGATCTTTTTTACTCTCCTACCCGCTGCCGGAACCGGTCCGGCTTCCCTTTCACGGAGGCGA
GCGCACCATACTCAAGCGCGATGCCATGTTCATCCGGGTGGAGGCCGATAGCGGATTGATCGGCTATGCGCCGGGACCGG
GCAGTGAACAGGCGCACAAGGCGATTCAGCAGACGATCGCGCCCTTTTTGGAGGACCGGGTGTTGGCCGATCCCGACGCG
CTGCGCGTGCTCTTTCTACAGGGCCCGGGGATCGATGCCGAACTGGCAAAGATTTATTGCGCGGTGGAGATCGCGCTGTA
CGACCTTGTGGGCAAGGCGCGCGGTATTCCGATTTCGGAGATGATTGGCGGTCGCGTCCGCGATACCATTCGGCTGTACG
GCAGCGCGGGCATGTACATGTCGCCTGAGGGCTACGCCGCGGAGGCCGCCGCGATCGCCGCATTGGGATTCCGCGCATAC
AAGATGCGGCCGGCCGGCGGACCGGAGCAGGACCTGGAGACGGTCCGCCAGATGCGCAAGGCTGTGGGTCCCGATTTCGA
CCTGATGGTGGACGCACACACGTGGTGGCGCATGGGAGACAGCAGCTACCCGGCCGATACCGTCGAGCAACTGGCGCGCG
ACATGTCGGCGTACCACATCGCTTGGCTGGAAGAGCCGCTTCCGCCTGACGACCATGCCGGTTATCTCGCGCTGAAGCAA
AAGGACCTGGTACTGCTCGCAACCGGCGAGCACGAACCGAATGAAGACCGCTATCTCGATCTGATCCTGACGCAGGCGGT
GGACTACATTCAGATGGATGTCTGCTGCCAGGGCGGTTATGCACTGGGGCGGCGGCTATTCGCGGAGATCGCGCGCCAGG
GACTGAGCTTCGCGTTCCACAGCTGGGGCACCGCGCTGGAAGTCATGGCCGCCGCACATCTCGGCATCTGCTGGCCGCAG
ATGATCGCGGAGTGGCTGGAGTACCCCTGCTATTCCAGTCCGGGGCGAGTGGGCATGTATCCTTTCCCACTAGCGGCGGA
GATTCTGACGGAGCCGCTGGAGATCGAACGCGGCGACCTGCTGGTTCCGCGTGCACCGGGGCTGGGTGTGGCGGTGGACG
AATCGGTGATCGAACGATATCCATGGATCCCGGGTCCGTGGTCGTACTTCCGGATCGATTCGCCGGCGGAGAATCGTGCG
GTGACTAGTGACCACAGCGTGCAGTGGGATCCCGAAGCGAAGTAG

Upstream 100 bases:

>100_bases
TGTGAGTGGCGGGCGCCGGCTTGAGGCCACACAAGAGATCGCTCGCTGACGGTCGCGTGTCGCGGGTTTGTGAGGACAAG
TGGTATCCTGGGCGCGACTG

Downstream 100 bases:

>100_bases
CGGCCATGCCGGCATCCGACGCCAATGTGATCGTGATCGGAGCCGGTGCGGCGGGACTTGCCGCGGCGCGCGATCTGCGG
CGAGCTGGGCGGAACGTCCT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKITSVRSFLLSYPLPEPVRLPFHGGERTILKRDAMFIRVEADSGLIGYAPGPGSEQAHKAIQQTIAPFLEDRVLADPDA
LRVLFLQGPGIDAELAKIYCAVEIALYDLVGKARGIPISEMIGGRVRDTIRLYGSAGMYMSPEGYAAEAAAIAALGFRAY
KMRPAGGPEQDLETVRQMRKAVGPDFDLMVDAHTWWRMGDSSYPADTVEQLARDMSAYHIAWLEEPLPPDDHAGYLALKQ
KDLVLLATGEHEPNEDRYLDLILTQAVDYIQMDVCCQGGYALGRRLFAEIARQGLSFAFHSWGTALEVMAAAHLGICWPQ
MIAEWLEYPCYSSPGRVGMYPFPLAAEILTEPLEIERGDLLVPRAPGLGVAVDESVIERYPWIPGPWSYFRIDSPAENRA
VTSDHSVQWDPEAK

Sequences:

>Translated_414_residues
MKITSVRSFLLSYPLPEPVRLPFHGGERTILKRDAMFIRVEADSGLIGYAPGPGSEQAHKAIQQTIAPFLEDRVLADPDA
LRVLFLQGPGIDAELAKIYCAVEIALYDLVGKARGIPISEMIGGRVRDTIRLYGSAGMYMSPEGYAAEAAAIAALGFRAY
KMRPAGGPEQDLETVRQMRKAVGPDFDLMVDAHTWWRMGDSSYPADTVEQLARDMSAYHIAWLEEPLPPDDHAGYLALKQ
KDLVLLATGEHEPNEDRYLDLILTQAVDYIQMDVCCQGGYALGRRLFAEIARQGLSFAFHSWGTALEVMAAAHLGICWPQ
MIAEWLEYPCYSSPGRVGMYPFPLAAEILTEPLEIERGDLLVPRAPGLGVAVDESVIERYPWIPGPWSYFRIDSPAENRA
VTSDHSVQWDPEAK
>Mature_414_residues
MKITSVRSFLLSYPLPEPVRLPFHGGERTILKRDAMFIRVEADSGLIGYAPGPGSEQAHKAIQQTIAPFLEDRVLADPDA
LRVLFLQGPGIDAELAKIYCAVEIALYDLVGKARGIPISEMIGGRVRDTIRLYGSAGMYMSPEGYAAEAAAIAALGFRAY
KMRPAGGPEQDLETVRQMRKAVGPDFDLMVDAHTWWRMGDSSYPADTVEQLARDMSAYHIAWLEEPLPPDDHAGYLALKQ
KDLVLLATGEHEPNEDRYLDLILTQAVDYIQMDVCCQGGYALGRRLFAEIARQGLSFAFHSWGTALEVMAAAHLGICWPQ
MIAEWLEYPCYSSPGRVGMYPFPLAAEILTEPLEIERGDLLVPRAPGLGVAVDESVIERYPWIPGPWSYFRIDSPAENRA
VTSDHSVQWDPEAK

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510960, Length=272, Percent_Identity=28.6764705882353, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994953, Length=366, Percent_Identity=22.1311475409836, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 45883; Mature: 45883

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITSVRSFLLSYPLPEPVRLPFHGGERTILKRDAMFIRVEADSGLIGYAPGPGSEQAHK
CCCHHHHHHHHHCCCCCCEECCCCCCCEEEEEECEEEEEEECCCCEEECCCCCCCHHHHH
AIQQTIAPFLEDRVLADPDALRVLFLQGPGIDAELAKIYCAVEIALYDLVGKARGIPISE
HHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MIGGRVRDTIRLYGSAGMYMSPEGYAAEAAAIAALGFRAYKMRPAGGPEQDLETVRQMRK
HHCCHHHHHHEEEECCCCEECCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
AVGPDFDLMVDAHTWWRMGDSSYPADTVEQLARDMSAYHIAWLEEPLPPDDHAGYLALKQ
HHCCCCEEEEECCHHEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEC
KDLVLLATGEHEPNEDRYLDLILTQAVDYIQMDVCCQGGYALGRRLFAEIARQGLSFAFH
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SWGTALEVMAAAHLGICWPQMIAEWLEYPCYSSPGRVGMYPFPLAAEILTEPLEIERGDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCE
LVPRAPGLGVAVDESVIERYPWIPGPWSYFRIDSPAENRAVTSDHSVQWDPEAK
EEECCCCCCEEECHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITSVRSFLLSYPLPEPVRLPFHGGERTILKRDAMFIRVEADSGLIGYAPGPGSEQAHK
CCCHHHHHHHHHCCCCCCEECCCCCCCEEEEEECEEEEEEECCCCEEECCCCCCCHHHHH
AIQQTIAPFLEDRVLADPDALRVLFLQGPGIDAELAKIYCAVEIALYDLVGKARGIPISE
HHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MIGGRVRDTIRLYGSAGMYMSPEGYAAEAAAIAALGFRAYKMRPAGGPEQDLETVRQMRK
HHCCHHHHHHEEEECCCCEECCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
AVGPDFDLMVDAHTWWRMGDSSYPADTVEQLARDMSAYHIAWLEEPLPPDDHAGYLALKQ
HHCCCCEEEEECCHHEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEC
KDLVLLATGEHEPNEDRYLDLILTQAVDYIQMDVCCQGGYALGRRLFAEIARQGLSFAFH
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SWGTALEVMAAAHLGICWPQMIAEWLEYPCYSSPGRVGMYPFPLAAEILTEPLEIERGDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCE
LVPRAPGLGVAVDESVIERYPWIPGPWSYFRIDSPAENRAVTSDHSVQWDPEAK
EEECCCCCCEEECHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA