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The map label for this gene is nadE [H]

Identifier: 116626533

GI number: 116626533

Start: 9390714

End: 9392348

Strand: Direct

Name: nadE [H]

Synonym: Acid_7496

Alternate gene names: 116626533

Gene position: 9390714-9392348 (Clockwise)

Preceding gene: 116626531

Following gene: 116626534

Centisome position: 94.23

GC content: 63.18

Gene sequence:

>1635_bases
TTGCGTCTAGCGCTTCTTCAGATCAACCCTACTGCAGGCGACCTTGAAGGGAACTCTTCGCTGATCATCCGGGCGGCCCG
GTCGGCGGAGGCTCAGGGGGCCGACCTGATGGCCACTCCCGAGCTCGCGCTGATGGGCTACCTGCCGCGCGACCTGCTCA
TGAACCAGGGCTTCGTGCGCCGCGGCTGCGAAAGACTTTCGCAGATTGCAAAGGAGTTGAAGGATGCTCCGCCGCTCCTC
GTCGGCGTGGCGACGCCCAACCCGTCGGACGTCGGCCGGCCGCTGTACAACAGCGCGGTGCTGCTTCAGAACGGTTCCGT
CGGGCCGGCCTTTCACAAGTCGCTCCTGCCCACTTACGATGTCTTCGACGAAGACCGCTATTTCGAGCCCTATCACGGCG
CCCAAATCCTCGAACTGAACGGCGTAAAGCTGGGCATCAGCATCTGCGAAGACGTCTGGAACGACCGCGATTTCTGGCAG
CGCCGCCGCTATCACCAGGACCCGATCGAAGAGCTGGCACAGGCCGGCGCGCAGGCGATCCTGAATCTCTCGGCCTCGCC
GTTCTCTGTCGGTAAGCAGGAACTCCGTGAGCGCATGCTCGGCCACATGGCGCAGAAGTACGGCCTGCCGGTGACGATCG
TGAACCAGGTCGGCGCCAATGACGATTTGATCTTCGATGGACGCAGCGCCGCCTTCGACGCGCAGGGCCGGTTGTTCGCG
CGCGCCAAAGGCTTCCAGGAAGATGTCCTGCTGGTGGATCTGCTCGGCAGTGAAGGCACGGTCGCGCCCGACGATTTCAC
CCCAGAGGCGGAGATCTGGAATGCGCTGGTGTTGGGCGTCCGCGATTATGCCCGCAAGACGCGCTTCCGCAAGGTGCTCC
TGGGACTCTCCGGCGGCATCGATTCGGCGCTGACGGCCGCCATCGCCGCCGATGCCATGGGCCCGGAGAACGTGCTCGGC
GTCATGATGCCTTCCGTTTACTCGAGCCAGGGCAGCGTGGACGATTCCGTGGAACTGGCCCGCAACCTCGGGATCCAGAC
CATGAAGCTGCCCATCGGCGGCATCATGAAGACCTACGATGGGGTCCTGGCGGATGCTTTCGCGGGGTTGAAGCCGGACG
TCACCGAAGAGAACATCCAATCGCGCATCCGCGGCAATCTGTTGATGGCGCTCTCCAATAAGTTCGGGTCGCTGCTGCTG
ACCACGGGCAACAAGAGCGAAATGTCGGTGGGGTACTGCACCCTCTACGGCGATATGAACGGCGGACTCGCGGTGATCGC
GGACCTGCCGAAAATGATGGTCTACCGCGTCTCCCGCTGGCGCAATTTGCGCGGTCCCGCGATTCCGGAATCCACCATCA
CCAAGCCGCCCTCCGCCGAACTACGGCCCGACCAGACCGATCAGGACAGCCTGCCGCCTTACGAGCTGCTGGACGAGATC
CTGGAACTTCACGTCGAGCAGTGCCAGAGCGCCGAGGAAATCATCGCGCAGGGATATGATGAGCAGACGGTGCGCAGGGT
ACTGCGCCTGGTGCGCATCGCCGAGTTTAAACGAAAGCAGGCCGCCCCGGTCTTGAAGGTCACATCACGCGCCTTCGGCA
CCGGCTGGCGGATGCCCATTGTCAGACAGGAATAA

Upstream 100 bases:

>100_bases
ACAGGCAGGAAAATCAACTTGTTGTTACACTGAGTGAAAGCCCTGATCTCTTTGAGAATTCTTGTGTGCAATTCTACTTT
TAACAGGGCGGGTACACGTT

Downstream 100 bases:

>100_bases
GCATGAAGGTATCGATCATCGGCCTAGGTAAACTAGGCGCTCCCATGGCCGCCGTGATGGCGCACAAAGGAAACTTCGTG
GTTGGTGTGGATATCAACCC

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MRLALLQINPTAGDLEGNSSLIIRAARSAEAQGADLMATPELALMGYLPRDLLMNQGFVRRGCERLSQIAKELKDAPPLL
VGVATPNPSDVGRPLYNSAVLLQNGSVGPAFHKSLLPTYDVFDEDRYFEPYHGAQILELNGVKLGISICEDVWNDRDFWQ
RRRYHQDPIEELAQAGAQAILNLSASPFSVGKQELRERMLGHMAQKYGLPVTIVNQVGANDDLIFDGRSAAFDAQGRLFA
RAKGFQEDVLLVDLLGSEGTVAPDDFTPEAEIWNALVLGVRDYARKTRFRKVLLGLSGGIDSALTAAIAADAMGPENVLG
VMMPSVYSSQGSVDDSVELARNLGIQTMKLPIGGIMKTYDGVLADAFAGLKPDVTEENIQSRIRGNLLMALSNKFGSLLL
TTGNKSEMSVGYCTLYGDMNGGLAVIADLPKMMVYRVSRWRNLRGPAIPESTITKPPSAELRPDQTDQDSLPPYELLDEI
LELHVEQCQSAEEIIAQGYDEQTVRRVLRLVRIAEFKRKQAAPVLKVTSRAFGTGWRMPIVRQE

Sequences:

>Translated_544_residues
MRLALLQINPTAGDLEGNSSLIIRAARSAEAQGADLMATPELALMGYLPRDLLMNQGFVRRGCERLSQIAKELKDAPPLL
VGVATPNPSDVGRPLYNSAVLLQNGSVGPAFHKSLLPTYDVFDEDRYFEPYHGAQILELNGVKLGISICEDVWNDRDFWQ
RRRYHQDPIEELAQAGAQAILNLSASPFSVGKQELRERMLGHMAQKYGLPVTIVNQVGANDDLIFDGRSAAFDAQGRLFA
RAKGFQEDVLLVDLLGSEGTVAPDDFTPEAEIWNALVLGVRDYARKTRFRKVLLGLSGGIDSALTAAIAADAMGPENVLG
VMMPSVYSSQGSVDDSVELARNLGIQTMKLPIGGIMKTYDGVLADAFAGLKPDVTEENIQSRIRGNLLMALSNKFGSLLL
TTGNKSEMSVGYCTLYGDMNGGLAVIADLPKMMVYRVSRWRNLRGPAIPESTITKPPSAELRPDQTDQDSLPPYELLDEI
LELHVEQCQSAEEIIAQGYDEQTVRRVLRLVRIAEFKRKQAAPVLKVTSRAFGTGWRMPIVRQE
>Mature_544_residues
MRLALLQINPTAGDLEGNSSLIIRAARSAEAQGADLMATPELALMGYLPRDLLMNQGFVRRGCERLSQIAKELKDAPPLL
VGVATPNPSDVGRPLYNSAVLLQNGSVGPAFHKSLLPTYDVFDEDRYFEPYHGAQILELNGVKLGISICEDVWNDRDFWQ
RRRYHQDPIEELAQAGAQAILNLSASPFSVGKQELRERMLGHMAQKYGLPVTIVNQVGANDDLIFDGRSAAFDAQGRLFA
RAKGFQEDVLLVDLLGSEGTVAPDDFTPEAEIWNALVLGVRDYARKTRFRKVLLGLSGGIDSALTAAIAADAMGPENVLG
VMMPSVYSSQGSVDDSVELARNLGIQTMKLPIGGIMKTYDGVLADAFAGLKPDVTEENIQSRIRGNLLMALSNKFGSLLL
TTGNKSEMSVGYCTLYGDMNGGLAVIADLPKMMVYRVSRWRNLRGPAIPESTITKPPSAELRPDQTDQDSLPPYELLDEI
LELHVEQCQSAEEIIAQGYDEQTVRRVLRLVRIAEFKRKQAAPVLKVTSRAFGTGWRMPIVRQE

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=600, Percent_Identity=25, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59689; Mature: 59689

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLALLQINPTAGDLEGNSSLIIRAARSAEAQGADLMATPELALMGYLPRDLLMNQGFVR
CEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCHHHHHCCCHHHHHCCCHHH
RGCERLSQIAKELKDAPPLLVGVATPNPSDVGRPLYNSAVLLQNGSVGPAFHKSLLPTYD
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCHHCCEEEEECCCCCHHHHHHCCCCCC
VFDEDRYFEPYHGAQILELNGVKLGISICEDVWNDRDFWQRRRYHQDPIEELAQAGAQAI
CCCCCCCCCCCCCCEEEEECCEEEHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHH
LNLSASPFSVGKQELRERMLGHMAQKYGLPVTIVNQVGANDDLIFDGRSAAFDAQGRLFA
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCCCCCCHH
RAKGFQEDVLLVDLLGSEGTVAPDDFTPEAEIWNALVLGVRDYARKTRFRKVLLGLSGGI
HHCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DSALTAAIAADAMGPENVLGVMMPSVYSSQGSVDDSVELARNLGIQTMKLPIGGIMKTYD
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHH
GVLADAFAGLKPDVTEENIQSRIRGNLLMALSNKFGSLLLTTGNKSEMSVGYCTLYGDMN
HHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCEEEEEEEEECCC
GGLAVIADLPKMMVYRVSRWRNLRGPAIPESTITKPPSAELRPDQTDQDSLPPYELLDEI
CCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LELHVEQCQSAEEIIAQGYDEQTVRRVLRLVRIAEFKRKQAAPVLKVTSRAFGTGWRMPI
HHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
VRQE
CCCC
>Mature Secondary Structure
MRLALLQINPTAGDLEGNSSLIIRAARSAEAQGADLMATPELALMGYLPRDLLMNQGFVR
CEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCHHHHHCCCHHHHHCCCHHH
RGCERLSQIAKELKDAPPLLVGVATPNPSDVGRPLYNSAVLLQNGSVGPAFHKSLLPTYD
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCHHCCEEEEECCCCCHHHHHHCCCCCC
VFDEDRYFEPYHGAQILELNGVKLGISICEDVWNDRDFWQRRRYHQDPIEELAQAGAQAI
CCCCCCCCCCCCCCEEEEECCEEEHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHH
LNLSASPFSVGKQELRERMLGHMAQKYGLPVTIVNQVGANDDLIFDGRSAAFDAQGRLFA
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCCCCCCCCCHH
RAKGFQEDVLLVDLLGSEGTVAPDDFTPEAEIWNALVLGVRDYARKTRFRKVLLGLSGGI
HHCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DSALTAAIAADAMGPENVLGVMMPSVYSSQGSVDDSVELARNLGIQTMKLPIGGIMKTYD
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHH
GVLADAFAGLKPDVTEENIQSRIRGNLLMALSNKFGSLLLTTGNKSEMSVGYCTLYGDMN
HHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCCCCCEEEECCCCCCCEEEEEEEEECCC
GGLAVIADLPKMMVYRVSRWRNLRGPAIPESTITKPPSAELRPDQTDQDSLPPYELLDEI
CCEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LELHVEQCQSAEEIIAQGYDEQTVRRVLRLVRIAEFKRKQAAPVLKVTSRAFGTGWRMPI
HHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
VRQE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]