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The map label for this gene is plcB [H]

Identifier: 116626368

GI number: 116626368

Start: 9201356

End: 9202684

Strand: Direct

Name: plcB [H]

Synonym: Acid_7328

Alternate gene names: 116626368

Gene position: 9201356-9202684 (Clockwise)

Preceding gene: 116626367

Following gene: 116626370

Centisome position: 92.33

GC content: 60.5

Gene sequence:

>1329_bases
ATGCCGTCCAGCGATCCGATTCGCCACGTTATTCTCCTCATCATGGAGAACCGTTCTTTTGATCACATGATGGGCGGCCT
CTCCACCGTCCTCCCGGGACTCGATGGAGTCGATCCACAACATCCCCACGTGAATTACGATGCCGCCGGCACGCCTTTCT
TTCAGGCGCCCAGCACGATCCGGCAAACCACGTCAGACCCTAAACACGAACACGCGGACGTCGTCATCCAACTGGCGAAC
AATAACGGCGGCTTTGTGCAGGATTTCATCAAGGCCTACCCGAACAGCACCACGCAAGAACGCCAGCAGATCATGGATTA
CTTCCCCGCCGATTTTCTGCCCGGCCTCCACTCACTGGCGCACGACTTCACCGTCTGCGACCGCTGGTACTCTTCCGTTC
CCGGCCCCACCTGGCCAAACCGCTTCTTCGCGCTCTCCGGAACCTCGCTCGGACGCATTCTGATGCCGGCCAGCGTCACC
ACCGATCCCGGCGCCTACTTTCAGCAGACCCAGATCACACTCTTCGACCGTCTCACTCAAGCCGGCAAGAGTTGGAAGAA
CTACTTCTGCGATTTCCCCGCCGCCTGGCTCCTGCTGCGCAACCTGCTTCCGCAAAATGTAGTGCACTCCCGTCCCATCG
ACGAGTTCTTTACCGCCGTGCGCGATGAGAAGTCCTTCCCCGAATTTGCCTTTATTGAACCGAAATATTTCGGCGCCGAC
GAGAACGATGATCACCCGCCGCACAACATCATGAAAGGCGAGAAGTTCATCGCCGACGTCTACAACGCCATCCGTTCCAA
CCCCGACCTGTGGAACAGCAGCCTCCTGGTGATCTTCTTCGATGAACACGGCGGCTTCTACGACCACGTCACACCCCCCG
GCGCCGTGGCCCCCGACGATCATCAGCACGAGTACACCTTCGACCAGCTCGGCGTGCGCGTGCCGTCCCTGTTGATCTCA
CCGTGGGTCTCCCGCGCGGTGGATCACACCCAGTACGATCACACTAGCGTCTTGAAGTACCTGATTGATAAATGGAAGCT
CGGCTCGCTGGGCCGCCGTGCTGCTGCGGCCACCAGCATCGCCGGAGCCCTTCGCGAATCCAAGCCCCGCACCGACACGG
TCCCGTTCATCCGCGTCCCGTACACCGTGCTCATGCCGGAGCACCCCGAATGGGAGACCGCCGCCGTCAGCGGCCACCAG
GATGGCCTCGAGCTCTTCGCGCAGCATCTGCAGTCGCAGATCAACGGCGTCCCTCAAACCATCACTCAACTCGCCGACAC
CGTGCGCACCGGAATCTTGGGAATCCCCGCTGCCCTCGGCATAAAGTAG

Upstream 100 bases:

>100_bases
TCCCGGAATTACGCGGCCGCGACGACGACGCCGTCCTCGCCTTCCTCCGCGCCCGCTGAGTTTACTACCGCCCTCGCTCC
GCCGTTATAGAATACCCTCC

Downstream 100 bases:

>100_bases
GTCGAGTTTCTCTTCGCCCTACCCCGCGATCGCGTCCGCCGCGTTGGGCTGGTAAATGCAGCGCGGATCCGACGCCAGGA
AATCGCCCGTCAGCGCATAC

Product: phospholipase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPSSDPIRHVILLIMENRSFDHMMGGLSTVLPGLDGVDPQHPHVNYDAAGTPFFQAPSTIRQTTSDPKHEHADVVIQLAN
NNGGFVQDFIKAYPNSTTQERQQIMDYFPADFLPGLHSLAHDFTVCDRWYSSVPGPTWPNRFFALSGTSLGRILMPASVT
TDPGAYFQQTQITLFDRLTQAGKSWKNYFCDFPAAWLLLRNLLPQNVVHSRPIDEFFTAVRDEKSFPEFAFIEPKYFGAD
ENDDHPPHNIMKGEKFIADVYNAIRSNPDLWNSSLLVIFFDEHGGFYDHVTPPGAVAPDDHQHEYTFDQLGVRVPSLLIS
PWVSRAVDHTQYDHTSVLKYLIDKWKLGSLGRRAAAATSIAGALRESKPRTDTVPFIRVPYTVLMPEHPEWETAAVSGHQ
DGLELFAQHLQSQINGVPQTITQLADTVRTGILGIPAALGIK

Sequences:

>Translated_442_residues
MPSSDPIRHVILLIMENRSFDHMMGGLSTVLPGLDGVDPQHPHVNYDAAGTPFFQAPSTIRQTTSDPKHEHADVVIQLAN
NNGGFVQDFIKAYPNSTTQERQQIMDYFPADFLPGLHSLAHDFTVCDRWYSSVPGPTWPNRFFALSGTSLGRILMPASVT
TDPGAYFQQTQITLFDRLTQAGKSWKNYFCDFPAAWLLLRNLLPQNVVHSRPIDEFFTAVRDEKSFPEFAFIEPKYFGAD
ENDDHPPHNIMKGEKFIADVYNAIRSNPDLWNSSLLVIFFDEHGGFYDHVTPPGAVAPDDHQHEYTFDQLGVRVPSLLIS
PWVSRAVDHTQYDHTSVLKYLIDKWKLGSLGRRAAAATSIAGALRESKPRTDTVPFIRVPYTVLMPEHPEWETAAVSGHQ
DGLELFAQHLQSQINGVPQTITQLADTVRTGILGIPAALGIK
>Mature_441_residues
PSSDPIRHVILLIMENRSFDHMMGGLSTVLPGLDGVDPQHPHVNYDAAGTPFFQAPSTIRQTTSDPKHEHADVVIQLANN
NGGFVQDFIKAYPNSTTQERQQIMDYFPADFLPGLHSLAHDFTVCDRWYSSVPGPTWPNRFFALSGTSLGRILMPASVTT
DPGAYFQQTQITLFDRLTQAGKSWKNYFCDFPAAWLLLRNLLPQNVVHSRPIDEFFTAVRDEKSFPEFAFIEPKYFGADE
NDDHPPHNIMKGEKFIADVYNAIRSNPDLWNSSLLVIFFDEHGGFYDHVTPPGAVAPDDHQHEYTFDQLGVRVPSLLISP
WVSRAVDHTQYDHTSVLKYLIDKWKLGSLGRRAAAATSIAGALRESKPRTDTVPFIRVPYTVLMPEHPEWETAAVSGHQD
GLELFAQHLQSQINGVPQTITQLADTVRTGILGIPAALGIK

Specific function: Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 49459; Mature: 49328

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSDPIRHVILLIMENRSFDHMMGGLSTVLPGLDGVDPQHPHVNYDAAGTPFFQAPSTI
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RQTTSDPKHEHADVVIQLANNNGGFVQDFIKAYPNSTTQERQQIMDYFPADFLPGLHSLA
HHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHH
HDFTVCDRWYSSVPGPTWPNRFFALSGTSLGRILMPASVTTDPGAYFQQTQITLFDRLTQ
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
AGKSWKNYFCDFPAAWLLLRNLLPQNVVHSRPIDEFFTAVRDEKSFPEFAFIEPKYFGAD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCCC
ENDDHPPHNIMKGEKFIADVYNAIRSNPDLWNSSLLVIFFDEHGGFYDHVTPPGAVAPDD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCCC
HQHEYTFDQLGVRVPSLLISPWVSRAVDHTQYDHTSVLKYLIDKWKLGSLGRRAAAATSI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AGALRESKPRTDTVPFIRVPYTVLMPEHPEWETAAVSGHQDGLELFAQHLQSQINGVPQT
HHHHHHCCCCCCCCCEEECCEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHH
ITQLADTVRTGILGIPAALGIK
HHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
PSSDPIRHVILLIMENRSFDHMMGGLSTVLPGLDGVDPQHPHVNYDAAGTPFFQAPSTI
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RQTTSDPKHEHADVVIQLANNNGGFVQDFIKAYPNSTTQERQQIMDYFPADFLPGLHSLA
HHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHH
HDFTVCDRWYSSVPGPTWPNRFFALSGTSLGRILMPASVTTDPGAYFQQTQITLFDRLTQ
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
AGKSWKNYFCDFPAAWLLLRNLLPQNVVHSRPIDEFFTAVRDEKSFPEFAFIEPKYFGAD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCCC
ENDDHPPHNIMKGEKFIADVYNAIRSNPDLWNSSLLVIFFDEHGGFYDHVTPPGAVAPDD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCCC
HQHEYTFDQLGVRVPSLLISPWVSRAVDHTQYDHTSVLKYLIDKWKLGSLGRRAAAATSI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AGALRESKPRTDTVPFIRVPYTVLMPEHPEWETAAVSGHQDGLELFAQHLQSQINGVPQT
HHHHHHCCCCCCCCCEEECCEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCHHH
ITQLADTVRTGILGIPAALGIK
HHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8757862; 9634230; 12218036 [H]