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The map label for this gene is clpB [H]

Identifier: 116626280

GI number: 116626280

Start: 9090056

End: 9091573

Strand: Direct

Name: clpB [H]

Synonym: Acid_7240

Alternate gene names: 116626280

Gene position: 9090056-9091573 (Clockwise)

Preceding gene: 116626279

Following gene: 116626282

Centisome position: 91.21

GC content: 59.35

Gene sequence:

>1518_bases
ATGAAGGATCCTGATCGTCCGGGTGAACGGCGGCGGCCGCGAGTCAAAAATGCCGACGCTGCAGTGCTGAAGTCGGAGAA
TGGTGCGGAGAAGGCCAAGACCGCGCAAAATCGAGTAAAACGGCAGCAATCTGACCCCGTTGAGGATCTCACCGCTGTCC
TCTCCCAAAAGGTCGTGGGTCAACCGGCTGCCACTCGAGTCATCGTCCCTTACATTCAGATGTTCCAGGCCGGTCTCGCT
CCCGAGGGCCGCCCGGTCGGTGTCTTCCTCTTGCTCGGTCCCACCGGCACCGGAAAAACCAAAACCATCGAGGCGCTTGC
CGACATCCTTCACGGCAGCGAAAAGAACGTCCTCAAAGTGGATTGCGGCGAATTTCAAATGGAGCACGAGGTCGCCAAAC
TCATCGGCGCGCCTCCCGGATATCTGGGCCATCGCGAAACTCAGCCGATGCTGACCCAGCAAAAACTAAACGCGGTCACC
AGTGAGAAATGTGCGCTTTCGCTCGTCCTCTTCGACGAGATCGAAAAGGCGGCGCCGTCCATGACGCGCCTGCTGCTCGG
CGTTCTCGATAAGGGATTGCTGCGGCTCGGCGACAATTCGGTAGTCAACTTCGAAAAGAGCCTGGTCTTCCTCACCAGCA
ATTTGGGCGCGCGTGAAATGATGCGTGAGCTCAACCCCGAGTTCGGCTTTCAGTCCGCGATCAGCCCGGAGCGCGCCGAT
CTTACCAGCAAGCTGCAAAACATCGCCTTGGTCGCCGTGCGCAAACGCTTCTCCCCGGAATTTGTCAATCGCATCGATTG
CATCATCACGTACCAGCCGCTGACCATTGAATCCCTCTCGACCATCCTCGACCAGCACATCATCGATCTTCAGAATCACG
TTAATACCAGACTAGGAAATCGAAGTTTCGCCCTCGACGTACCCTTCGAGACCCGCCAGTTCCTGCTGCAAAAAGGCACC
AGCGCGGAGTACGGCGCGCGCGAATTGAATCGCACCATCCACCGGTACCTCACCCAGCCGCTCGCGACCATGGTGGCAAC
CAACCAGATCGCCCCCGGAGTCGGCGTGGTCGCAGCGATTGGACCCGGAGACAAACTTGAGCTCCGCACCACAGGGGGAC
CTGGCCCGGTTGCCGCCCATCCCACTGTTCTGCTGGTGGACGACAATCGCGATCTGCTGCACTTCCTGGAACGCTTGATG
GCCGATGCCGGCTGGACTTTATTGACCGCCGAATCGGCCACCGAAGCGAAGAGACTGATGCAGGAGCACAAGCCTAATGC
CGCGCTCCTGGATTACATGCTGCCCGATGGCAACGGAGTCGAACTCGGCGTCGAGTTCCTGCAGAACGTGCCGCAGATGC
TGGTCATCGTAATGACCGGCACCATCCTCCCGCCGGAAGAGGAAGCGCTCTGCGAAGAACACAATTTCCCGGTGCTTCGC
AAACCCTTCCTCGCATCCGACGTGATGAACCAGATCCGCAGCCGGCTTATGCCGGTCACCGGCGTCGTGCGCGCGTAG

Upstream 100 bases:

>100_bases
ATGTTACATGTGTCCACTGGCACTCGCAACTTTGAAATAGTTTTCAGCAATTTCACTTCTAAGGTAAATTGTTGAATTAT
TATGGGGAAAGAGGCTCTTC

Downstream 100 bases:

>100_bases
AGTTACATCCGCGCGCCGCGCCCCAGATGGACCGCGCCGCCGCCGCCGATTGCTCGACCCGGCCGGACTACCATGGGTGC
GCCACGAAAACCGCCCCGGA

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MKDPDRPGERRRPRVKNADAAVLKSENGAEKAKTAQNRVKRQQSDPVEDLTAVLSQKVVGQPAATRVIVPYIQMFQAGLA
PEGRPVGVFLLLGPTGTGKTKTIEALADILHGSEKNVLKVDCGEFQMEHEVAKLIGAPPGYLGHRETQPMLTQQKLNAVT
SEKCALSLVLFDEIEKAAPSMTRLLLGVLDKGLLRLGDNSVVNFEKSLVFLTSNLGAREMMRELNPEFGFQSAISPERAD
LTSKLQNIALVAVRKRFSPEFVNRIDCIITYQPLTIESLSTILDQHIIDLQNHVNTRLGNRSFALDVPFETRQFLLQKGT
SAEYGARELNRTIHRYLTQPLATMVATNQIAPGVGVVAAIGPGDKLELRTTGGPGPVAAHPTVLLVDDNRDLLHFLERLM
ADAGWTLLTAESATEAKRLMQEHKPNAALLDYMLPDGNGVELGVEFLQNVPQMLVIVMTGTILPPEEEALCEEHNFPVLR
KPFLASDVMNQIRSRLMPVTGVVRA

Sequences:

>Translated_505_residues
MKDPDRPGERRRPRVKNADAAVLKSENGAEKAKTAQNRVKRQQSDPVEDLTAVLSQKVVGQPAATRVIVPYIQMFQAGLA
PEGRPVGVFLLLGPTGTGKTKTIEALADILHGSEKNVLKVDCGEFQMEHEVAKLIGAPPGYLGHRETQPMLTQQKLNAVT
SEKCALSLVLFDEIEKAAPSMTRLLLGVLDKGLLRLGDNSVVNFEKSLVFLTSNLGAREMMRELNPEFGFQSAISPERAD
LTSKLQNIALVAVRKRFSPEFVNRIDCIITYQPLTIESLSTILDQHIIDLQNHVNTRLGNRSFALDVPFETRQFLLQKGT
SAEYGARELNRTIHRYLTQPLATMVATNQIAPGVGVVAAIGPGDKLELRTTGGPGPVAAHPTVLLVDDNRDLLHFLERLM
ADAGWTLLTAESATEAKRLMQEHKPNAALLDYMLPDGNGVELGVEFLQNVPQMLVIVMTGTILPPEEEALCEEHNFPVLR
KPFLASDVMNQIRSRLMPVTGVVRA
>Mature_505_residues
MKDPDRPGERRRPRVKNADAAVLKSENGAEKAKTAQNRVKRQQSDPVEDLTAVLSQKVVGQPAATRVIVPYIQMFQAGLA
PEGRPVGVFLLLGPTGTGKTKTIEALADILHGSEKNVLKVDCGEFQMEHEVAKLIGAPPGYLGHRETQPMLTQQKLNAVT
SEKCALSLVLFDEIEKAAPSMTRLLLGVLDKGLLRLGDNSVVNFEKSLVFLTSNLGAREMMRELNPEFGFQSAISPERAD
LTSKLQNIALVAVRKRFSPEFVNRIDCIITYQPLTIESLSTILDQHIIDLQNHVNTRLGNRSFALDVPFETRQFLLQKGT
SAEYGARELNRTIHRYLTQPLATMVATNQIAPGVGVVAAIGPGDKLELRTTGGPGPVAAHPTVLLVDDNRDLLHFLERLM
ADAGWTLLTAESATEAKRLMQEHKPNAALLDYMLPDGNGVELGVEFLQNVPQMLVIVMTGTILPPEEEALCEEHNFPVLR
KPFLASDVMNQIRSRLMPVTGVVRA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=351, Percent_Identity=27.0655270655271, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788943, Length=300, Percent_Identity=33.3333333333333, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1787109, Length=354, Percent_Identity=32.4858757062147, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320464, Length=303, Percent_Identity=37.6237623762376, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323002, Length=310, Percent_Identity=30, Blast_Score=149, Evalue=7e-37,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 55471; Mature: 55471

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDPDRPGERRRPRVKNADAAVLKSENGAEKAKTAQNRVKRQQSDPVEDLTAVLSQKVVG
CCCCCCCCCCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
QPAATRVIVPYIQMFQAGLAPEGRPVGVFLLLGPTGTGKTKTIEALADILHGSEKNVLKV
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEE
DCGEFQMEHEVAKLIGAPPGYLGHRETQPMLTQQKLNAVTSEKCALSLVLFDEIEKAAPS
ECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
MTRLLLGVLDKGLLRLGDNSVVNFEKSLVFLTSNLGAREMMRELNPEFGFQSAISPERAD
HHHHHHHHHHHHHHHCCCCCEEEHHHEEEEEECCCCHHHHHHHCCCCCCCHHCCCCHHHH
LTSKLQNIALVAVRKRFSPEFVNRIDCIITYQPLTIESLSTILDQHIIDLQNHVNTRLGN
HHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
RSFALDVPFETRQFLLQKGTSAEYGARELNRTIHRYLTQPLATMVATNQIAPGVGVVAAI
CEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
GPGDKLELRTTGGPGPVAAHPTVLLVDDNRDLLHFLERLMADAGWTLLTAESATEAKRLM
CCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHH
QEHKPNAALLDYMLPDGNGVELGVEFLQNVPQMLVIVMTGTILPPEEEALCEEHNFPVLR
HHCCCCCEEHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
KPFLASDVMNQIRSRLMPVTGVVRA
CCHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MKDPDRPGERRRPRVKNADAAVLKSENGAEKAKTAQNRVKRQQSDPVEDLTAVLSQKVVG
CCCCCCCCCCCCCCCCCCCHHEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
QPAATRVIVPYIQMFQAGLAPEGRPVGVFLLLGPTGTGKTKTIEALADILHGSEKNVLKV
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEE
DCGEFQMEHEVAKLIGAPPGYLGHRETQPMLTQQKLNAVTSEKCALSLVLFDEIEKAAPS
ECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
MTRLLLGVLDKGLLRLGDNSVVNFEKSLVFLTSNLGAREMMRELNPEFGFQSAISPERAD
HHHHHHHHHHHHHHHCCCCCEEEHHHEEEEEECCCCHHHHHHHCCCCCCCHHCCCCHHHH
LTSKLQNIALVAVRKRFSPEFVNRIDCIITYQPLTIESLSTILDQHIIDLQNHVNTRLGN
HHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
RSFALDVPFETRQFLLQKGTSAEYGARELNRTIHRYLTQPLATMVATNQIAPGVGVVAAI
CEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
GPGDKLELRTTGGPGPVAAHPTVLLVDDNRDLLHFLERLMADAGWTLLTAESATEAKRLM
CCCCEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHH
QEHKPNAALLDYMLPDGNGVELGVEFLQNVPQMLVIVMTGTILPPEEEALCEEHNFPVLR
HHCCCCCEEHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
KPFLASDVMNQIRSRLMPVTGVVRA
CCHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]