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The map label for this gene is glgX [H]

Identifier: 116626210

GI number: 116626210

Start: 9000871

End: 9003000

Strand: Direct

Name: glgX [H]

Synonym: Acid_7170

Alternate gene names: 116626210

Gene position: 9000871-9003000 (Clockwise)

Preceding gene: 116626209

Following gene: 116626215

Centisome position: 90.32

GC content: 61.78

Gene sequence:

>2130_bases
ATGCTGGAACCGCACACCACGAAAGCGGTGGAGCCCGGCTCCCCCTTTCCCCTTGGCGCGACGCTGGTTGACGGCGGCGT
GAACTTTGCCATCTACTCCAAACAAGCGGCCGGGGTTTTTCTACTGCTGTTCGATTCCGCGGACGGCGCGGCGACGGATG
TCATCCCACTAACCGCTCGCGACAAGCTGGTATGGCACGCGTGCGTCAAGGGCGTTCGCGCGGGACAGCTTTACGGGTAC
AAAGTTAGCGGGGAATACCGGCCGGATCTCGGCCTGCGCTTCAATGAGGCTAAACTGCTGCTGGATCCGTACGCCAGGGC
GGTCACGGGGAAGTTCCGCAATGTGGATAACCTGCTGCTCGCGTACGCTGCGCAGCCCGCGTCGGCAGACCTCACGCAGG
ACACGCGCGACAACAGCGCGGTGGTGCCGAAGGGCATCGTGATCGACGACGGTTTCGACTGGCAGGGCGTGGGCGCGCCA
AAATCCGCGCTGGAACAATTGGTGATCTACGAGGTCCACGTCAAGGGGTTCACGGCGCATCCATCATCCGGCGTAAGCGC
TCCGGGGACATACCTGGGATTCATCGAGAAAATCCCGCACCTGACCAGGCTCGGTGTGAATGCGGTCGAGTTGATGCCTG
TGCACGAGCACTACGTCGATGACTTTCTAGTCGATCGCGGGCTGACAAATTACTGGGGTTACAACACGATTGGATTTTTC
GCGCCGGAAGTGTCGTACGGCAGCCAGCGTGAAGCGGGATGCCAGGTAGCGGAGTTCAAGACGCTCGTGCGGGAACTGCA
TCGCGCTGGGATCCAGGTGATACTCGACGTGGTTTACAACCACACAGCGGAGGGCAACGAGATGGGCCCGAGCATGAGTT
TTCGCGGGCTGGACAACGTCTCGTATTACTGCCTCACAGGTCCGGCGGAGGCGCCGCGGCGCTACTACATGAACTATACG
GGCTGCGGCAACAGCATGAATTTCGACAGTCCGGCGGTAATCCGGCTGGTGATGGACTCGCTGCGTTATTGGGTGGAGGC
GATGCACGTGGACGGGTTCCGGTTCGACCTGGCGAGCGTGCTGGGGCGCGGTGCCGACGGCAGTTTCCGGTCGACGTCGG
GATTCTTCGACGCGGTGGCGCAGGATCCCGTGTTGAGCCGCGCGATTCTGATCGCTGAGCCGTGGGATACCGGGACGTAC
CAGCTCGGGAATTTTCCAATCGACTGGTCAGAGTGGAATGGAAAGTTCCGCGACACGGTGCGGTGTTTCGCGAAGAGCGA
CTCCGGACAGTTGTCGCAGATGGGCTGGCGGCTCACGGGGTCGGCGGATTTGTATGGCAGCGAAGGGCGGTCGGCGTATA
ACAGCGTCAACTTCGTGACCTGCCACGACGGGTTCACGCTGCACGACCTGGTTTCCTATAACGGGAAGCACAATGAGGGC
AACGGTGAGAACAACCAGGACGGAACCAACGACAACAACTCATGGAATTGCGGCGCGGAAGGGGACACGACGGATTCCGG
GATCCTGTCGCTCCGCCGGCAGCTAATGAGGAATCACGCGTGCTACCTGATGTTCGCGTGCGGCACTCCGATGATGCTCG
GCGGAGACGAATTCGCACGGAGCCAGCAGGGGAACAACAACGCGTACTGCCAGGACAACGCGATTGGCTGGTTCGACTGG
AGCCTGGTAGAGCGCAACGGCGACCTGGTGGAATTCTTTCGGAAGGCGATTGCTTTCACCCGGCGCTTCCCGGTGCTGCA
ACGCCGGAAGTTTCTGCTGGGGGACGATCTGGACGCGGACGGGGTCGCCGACCTGACCTGGTTTTCGCCGGACCTGGGTA
CTCCGGCATGGTCGGATGCGAATGCGCGCGTGCTCTGCTACCAGCTGGATACGTCCGACTGCGGCGACACATGCGATGCC
GACCGGCTGTTCTTCATTCTCAACAGCGACTTCGATTCGCAGTGGGTGAAGCTGCCGCCGCTCGCGGCCGGACACACATG
GTTCCGTGCGATTGACACGAGCTTGGCCGCGGGCGAGGACTTCGCGGAGAACGGCACGGAAGTCCGCGTGGAGCCGGACG
ACCACTACATTGCGAATGCCAGGAGCACGGTAGTGCTCCTGGCGCGGTGA

Upstream 100 bases:

>100_bases
ATCCCGTAACGTGGATCCTGATGGTGGGCCGAGGCGCGCCGGGACTGATTTGCCCTTACCAAAATCGCGACCTCGCGGTA
TGCAGGATGTAAGATGGAGC

Downstream 100 bases:

>100_bases
GAGGGCTATTACTGGTGGTCGGGACGGCAGGTGTTCCCACTGCCACTGCAATCGTAGGACAGTTCCAAAGCGGTGTAGGG
CCCCGTAAGGTCAAAATCGT

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MLEPHTTKAVEPGSPFPLGATLVDGGVNFAIYSKQAAGVFLLLFDSADGAATDVIPLTARDKLVWHACVKGVRAGQLYGY
KVSGEYRPDLGLRFNEAKLLLDPYARAVTGKFRNVDNLLLAYAAQPASADLTQDTRDNSAVVPKGIVIDDGFDWQGVGAP
KSALEQLVIYEVHVKGFTAHPSSGVSAPGTYLGFIEKIPHLTRLGVNAVELMPVHEHYVDDFLVDRGLTNYWGYNTIGFF
APEVSYGSQREAGCQVAEFKTLVRELHRAGIQVILDVVYNHTAEGNEMGPSMSFRGLDNVSYYCLTGPAEAPRRYYMNYT
GCGNSMNFDSPAVIRLVMDSLRYWVEAMHVDGFRFDLASVLGRGADGSFRSTSGFFDAVAQDPVLSRAILIAEPWDTGTY
QLGNFPIDWSEWNGKFRDTVRCFAKSDSGQLSQMGWRLTGSADLYGSEGRSAYNSVNFVTCHDGFTLHDLVSYNGKHNEG
NGENNQDGTNDNNSWNCGAEGDTTDSGILSLRRQLMRNHACYLMFACGTPMMLGGDEFARSQQGNNNAYCQDNAIGWFDW
SLVERNGDLVEFFRKAIAFTRRFPVLQRRKFLLGDDLDADGVADLTWFSPDLGTPAWSDANARVLCYQLDTSDCGDTCDA
DRLFFILNSDFDSQWVKLPPLAAGHTWFRAIDTSLAAGEDFAENGTEVRVEPDDHYIANARSTVVLLAR

Sequences:

>Translated_709_residues
MLEPHTTKAVEPGSPFPLGATLVDGGVNFAIYSKQAAGVFLLLFDSADGAATDVIPLTARDKLVWHACVKGVRAGQLYGY
KVSGEYRPDLGLRFNEAKLLLDPYARAVTGKFRNVDNLLLAYAAQPASADLTQDTRDNSAVVPKGIVIDDGFDWQGVGAP
KSALEQLVIYEVHVKGFTAHPSSGVSAPGTYLGFIEKIPHLTRLGVNAVELMPVHEHYVDDFLVDRGLTNYWGYNTIGFF
APEVSYGSQREAGCQVAEFKTLVRELHRAGIQVILDVVYNHTAEGNEMGPSMSFRGLDNVSYYCLTGPAEAPRRYYMNYT
GCGNSMNFDSPAVIRLVMDSLRYWVEAMHVDGFRFDLASVLGRGADGSFRSTSGFFDAVAQDPVLSRAILIAEPWDTGTY
QLGNFPIDWSEWNGKFRDTVRCFAKSDSGQLSQMGWRLTGSADLYGSEGRSAYNSVNFVTCHDGFTLHDLVSYNGKHNEG
NGENNQDGTNDNNSWNCGAEGDTTDSGILSLRRQLMRNHACYLMFACGTPMMLGGDEFARSQQGNNNAYCQDNAIGWFDW
SLVERNGDLVEFFRKAIAFTRRFPVLQRRKFLLGDDLDADGVADLTWFSPDLGTPAWSDANARVLCYQLDTSDCGDTCDA
DRLFFILNSDFDSQWVKLPPLAAGHTWFRAIDTSLAAGEDFAENGTEVRVEPDDHYIANARSTVVLLAR
>Mature_709_residues
MLEPHTTKAVEPGSPFPLGATLVDGGVNFAIYSKQAAGVFLLLFDSADGAATDVIPLTARDKLVWHACVKGVRAGQLYGY
KVSGEYRPDLGLRFNEAKLLLDPYARAVTGKFRNVDNLLLAYAAQPASADLTQDTRDNSAVVPKGIVIDDGFDWQGVGAP
KSALEQLVIYEVHVKGFTAHPSSGVSAPGTYLGFIEKIPHLTRLGVNAVELMPVHEHYVDDFLVDRGLTNYWGYNTIGFF
APEVSYGSQREAGCQVAEFKTLVRELHRAGIQVILDVVYNHTAEGNEMGPSMSFRGLDNVSYYCLTGPAEAPRRYYMNYT
GCGNSMNFDSPAVIRLVMDSLRYWVEAMHVDGFRFDLASVLGRGADGSFRSTSGFFDAVAQDPVLSRAILIAEPWDTGTY
QLGNFPIDWSEWNGKFRDTVRCFAKSDSGQLSQMGWRLTGSADLYGSEGRSAYNSVNFVTCHDGFTLHDLVSYNGKHNEG
NGENNQDGTNDNNSWNCGAEGDTTDSGILSLRRQLMRNHACYLMFACGTPMMLGGDEFARSQQGNNNAYCQDNAIGWFDW
SLVERNGDLVEFFRKAIAFTRRFPVLQRRKFLLGDDLDADGVADLTWFSPDLGTPAWSDANARVLCYQLDTSDCGDTCDA
DRLFFILNSDFDSQWVKLPPLAAGHTWFRAIDTSLAAGEDFAENGTEVRVEPDDHYIANARSTVVLLAR

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=218, Percent_Identity=30.7339449541284, Blast_Score=70, Evalue=9e-12,
Organism=Escherichia coli, GI2367229, Length=666, Percent_Identity=39.4894894894895, Blast_Score=448, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320826, Length=220, Percent_Identity=31.8181818181818, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78161; Mature: 78161

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPHTTKAVEPGSPFPLGATLVDGGVNFAIYSKQAAGVFLLLFDSADGAATDVIPLTAR
CCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEECCC
DKLVWHACVKGVRAGQLYGYKVSGEYRPDLGLRFNEAKLLLDPYARAVTGKFRNVDNLLL
CHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEECCCEEEECHHHHHHHHHHCCHHHEEE
AYAAQPASADLTQDTRDNSAVVPKGIVIDDGFDWQGVGAPKSALEQLVIYEVHVKGFTAH
EEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHEEEEEEEEEEEEEC
PSSGVSAPGTYLGFIEKIPHLTRLGVNAVELMPVHEHYVDDFLVDRGLTNYWGYNTIGFF
CCCCCCCCHHHHHHHHHCCHHHHCCCCEEEEEECHHHHHHHHHHHCCCCHHCCCCCCEEE
APEVSYGSQREAGCQVAEFKTLVRELHRAGIQVILDVVYNHTAEGNEMGPSMSFRGLDNV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCC
SYYCLTGPAEAPRRYYMNYTGCGNSMNFDSPAVIRLVMDSLRYWVEAMHVDGFRFDLASV
EEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LGRGADGSFRSTSGFFDAVAQDPVLSRAILIAEPWDTGTYQLGNFPIDWSEWNGKFRDTV
HCCCCCCCCCCCCCHHHHHHCCCHHEEEEEEECCCCCCCEECCCCCCCHHCCCCCHHHHH
RCFAKSDSGQLSQMGWRLTGSADLYGSEGRSAYNSVNFVTCHDGFTLHDLVSYNGKHNEG
HHHHCCCCCCHHHCCEEEECCCCCCCCCCCCHHCCCEEEEECCCCCHHHHHHCCCCCCCC
NGENNQDGTNDNNSWNCGAEGDTTDSGILSLRRQLMRNHACYLMFACGTPMMLGGDEFAR
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEECCHHHHH
SQQGNNNAYCQDNAIGWFDWSLVERNGDLVEFFRKAIAFTRRFPVLQRRKFLLGDDLDAD
HCCCCCCCEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
GVADLTWFSPDLGTPAWSDANARVLCYQLDTSDCGDTCDADRLFFILNSDFDSQWVKLPP
CCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEECCC
LAAGHTWFRAIDTSLAAGEDFAENGTEVRVEPDDHYIANARSTVVLLAR
CCCCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCEEECCCCEEEEEEC
>Mature Secondary Structure
MLEPHTTKAVEPGSPFPLGATLVDGGVNFAIYSKQAAGVFLLLFDSADGAATDVIPLTAR
CCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCEEEEEEECCCCCCCEEEEECCC
DKLVWHACVKGVRAGQLYGYKVSGEYRPDLGLRFNEAKLLLDPYARAVTGKFRNVDNLLL
CHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEECCCEEEECHHHHHHHHHHCCHHHEEE
AYAAQPASADLTQDTRDNSAVVPKGIVIDDGFDWQGVGAPKSALEQLVIYEVHVKGFTAH
EEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHEEEEEEEEEEEEEC
PSSGVSAPGTYLGFIEKIPHLTRLGVNAVELMPVHEHYVDDFLVDRGLTNYWGYNTIGFF
CCCCCCCCHHHHHHHHHCCHHHHCCCCEEEEEECHHHHHHHHHHHCCCCHHCCCCCCEEE
APEVSYGSQREAGCQVAEFKTLVRELHRAGIQVILDVVYNHTAEGNEMGPSMSFRGLDNV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCC
SYYCLTGPAEAPRRYYMNYTGCGNSMNFDSPAVIRLVMDSLRYWVEAMHVDGFRFDLASV
EEEEEECCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LGRGADGSFRSTSGFFDAVAQDPVLSRAILIAEPWDTGTYQLGNFPIDWSEWNGKFRDTV
HCCCCCCCCCCCCCHHHHHHCCCHHEEEEEEECCCCCCCEECCCCCCCHHCCCCCHHHHH
RCFAKSDSGQLSQMGWRLTGSADLYGSEGRSAYNSVNFVTCHDGFTLHDLVSYNGKHNEG
HHHHCCCCCCHHHCCEEEECCCCCCCCCCCCHHCCCEEEEECCCCCHHHHHHCCCCCCCC
NGENNQDGTNDNNSWNCGAEGDTTDSGILSLRRQLMRNHACYLMFACGTPMMLGGDEFAR
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEECCHHHHH
SQQGNNNAYCQDNAIGWFDWSLVERNGDLVEFFRKAIAFTRRFPVLQRRKFLLGDDLDAD
HCCCCCCCEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
GVADLTWFSPDLGTPAWSDANARVLCYQLDTSDCGDTCDADRLFFILNSDFDSQWVKLPP
CCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCEEECCC
LAAGHTWFRAIDTSLAAGEDFAENGTEVRVEPDDHYIANARSTVVLLAR
CCCCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]