Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 116626200

GI number: 116626200

Start: 8985366

End: 8986787

Strand: Direct

Name: gatA [H]

Synonym: Acid_7160

Alternate gene names: 116626200

Gene position: 8985366-8986787 (Clockwise)

Preceding gene: 116626199

Following gene: 116626204

Centisome position: 90.16

GC content: 63.99

Gene sequence:

>1422_bases
ATGGCCGCCATCGATCCCGACGTCTACTTCGCCACCATCCCGGAATTGAACGAGCGTCTCAAATCCAAGGAATTCACCGC
CGAGCAGTTGGCGCGCGCATTTTCCGCGCGGCTCCAGGAACTGGGACCGCGCTATAACGCGCTGGCCCTCTCTTTGAACC
AGGAGGCTCTACGGCAGGCCAAAGCCGTGGACAATGAACTCAAGCGCGATCGCTTTCGGGGTCCGCTGCAGGGCATTCCT
TATGGCGTCAAAGATCTGCTGGCCTATGCCGGACAGCCTACGACCTGGGGCGCGAAACCGTACGCGGGACAGGTGTTCGA
TTACAACGCCGCGGTGATAGACAAGATCTCCGCGCTGGGCGGAGTGCTGACCGGCAAGCTGGCGATGGTGGAGCTGGCGG
GCGGCGGCGGTTACCGGTTTGCCTCGGCGTCGCTGACGGGTCCGGGTTTGAATCCGTGGGATCGCACGCGATGGTCGGGC
GGATCGTCCAGCGGTTCGGCGGCGGCGGTGGCGGCCGGCCTGGTGCCGTACGCGATCGGCTCGGAGACTTCAGGCTCCAT
CGTCACGCCCGCAAGCTTTTGCGGAGTAACCGCGCTGCGTCCCACATATGGATTGGTCAGCCGTTACGGAGCGATGGCGC
TTTCCTGGACGCTGGACAAACTGGGGCCGTTCGCGCACACCGCGGAGGATTGCGGTCTCATCCTGCAGGCGATCGCCGGA
AAGGATTCGCGCGACCCCGGCTCGGCGGGGAAAAGTTTTTACTACACGCCGCAATATGCGCACCCGATGAAGGATTTGAA
GGTAGGGTATGCGCCGTCGGATTTCAGCGAATGGGCCGAACCTGCGGCGCGTCCCGCATTCGATGCCGCGCTCGGCGTGC
TGAAGGCGAGCGGAGTGCAGTTAGTGGAAACCAGGCTGCCGGACTTTCCCTACGGTGCGGCACTCTCCACGATTCTCAAC
GGCGAGATGGGCTCGATCTTCGAACCGCTGATCGCCAGCGGCAAAGTGGACGAACTAGCGGACAAGCCGCAGATCGCGGG
GCTTAAGGCGAGCCTGGAGCTGCCCGCGAAGGATTATCTGAAGGCGATGCGCATGCGGCGCATGATCCAGGATGAAGTCA
GCAAGCTCTTCGGCAGCATCGATGTGCTGCTGGCGCCGGGACGTCCCGGCATCGCGAGCAAGATCGACCAGCCCTTGGAT
CGCGGAGCGGGCGGCGCGCCGCCGAAAGATCCGGGTTTCCGCGCGATTATCCAGATGGGCAACCTCGCCGGATTGCCGGC
GCTGGTGCTGCCCTGCGGCTTCGCGGAGAACATGCCGGTGGCGCTGCAAGTGGTCGGCGTGCCGTTCTCCGAGAACACGC
TGCTCGCCATCGGGCGCGAATTCCAGAAGCGCACGGACTGGCACAAGCGTCATCCCGCATAG

Upstream 100 bases:

>100_bases
GGCACGCGACCGCATGCTGGCGAACGCCGCGGCCTTGGCAACACCGGCCATCCCGATGGCCACCGAACCCGCCTTCCAAT
TCAAGGCCTGAGGAGCATTG

Downstream 100 bases:

>100_bases
TTTATTTGGGCTCGAATTCGGTGGCGCACCGGCTGAGGGGTTCGACCCAACCTTTCGTTAGACTCGTGGTTCCCGCACCG
GTTGAAGGTTGGCGCTGGAC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAAIDPDVYFATIPELNERLKSKEFTAEQLARAFSARLQELGPRYNALALSLNQEALRQAKAVDNELKRDRFRGPLQGIP
YGVKDLLAYAGQPTTWGAKPYAGQVFDYNAAVIDKISALGGVLTGKLAMVELAGGGGYRFASASLTGPGLNPWDRTRWSG
GSSSGSAAAVAAGLVPYAIGSETSGSIVTPASFCGVTALRPTYGLVSRYGAMALSWTLDKLGPFAHTAEDCGLILQAIAG
KDSRDPGSAGKSFYYTPQYAHPMKDLKVGYAPSDFSEWAEPAARPAFDAALGVLKASGVQLVETRLPDFPYGAALSTILN
GEMGSIFEPLIASGKVDELADKPQIAGLKASLELPAKDYLKAMRMRRMIQDEVSKLFGSIDVLLAPGRPGIASKIDQPLD
RGAGGAPPKDPGFRAIIQMGNLAGLPALVLPCGFAENMPVALQVVGVPFSENTLLAIGREFQKRTDWHKRHPA

Sequences:

>Translated_473_residues
MAAIDPDVYFATIPELNERLKSKEFTAEQLARAFSARLQELGPRYNALALSLNQEALRQAKAVDNELKRDRFRGPLQGIP
YGVKDLLAYAGQPTTWGAKPYAGQVFDYNAAVIDKISALGGVLTGKLAMVELAGGGGYRFASASLTGPGLNPWDRTRWSG
GSSSGSAAAVAAGLVPYAIGSETSGSIVTPASFCGVTALRPTYGLVSRYGAMALSWTLDKLGPFAHTAEDCGLILQAIAG
KDSRDPGSAGKSFYYTPQYAHPMKDLKVGYAPSDFSEWAEPAARPAFDAALGVLKASGVQLVETRLPDFPYGAALSTILN
GEMGSIFEPLIASGKVDELADKPQIAGLKASLELPAKDYLKAMRMRRMIQDEVSKLFGSIDVLLAPGRPGIASKIDQPLD
RGAGGAPPKDPGFRAIIQMGNLAGLPALVLPCGFAENMPVALQVVGVPFSENTLLAIGREFQKRTDWHKRHPA
>Mature_472_residues
AAIDPDVYFATIPELNERLKSKEFTAEQLARAFSARLQELGPRYNALALSLNQEALRQAKAVDNELKRDRFRGPLQGIPY
GVKDLLAYAGQPTTWGAKPYAGQVFDYNAAVIDKISALGGVLTGKLAMVELAGGGGYRFASASLTGPGLNPWDRTRWSGG
SSSGSAAAVAAGLVPYAIGSETSGSIVTPASFCGVTALRPTYGLVSRYGAMALSWTLDKLGPFAHTAEDCGLILQAIAGK
DSRDPGSAGKSFYYTPQYAHPMKDLKVGYAPSDFSEWAEPAARPAFDAALGVLKASGVQLVETRLPDFPYGAALSTILNG
EMGSIFEPLIASGKVDELADKPQIAGLKASLELPAKDYLKAMRMRRMIQDEVSKLFGSIDVLLAPGRPGIASKIDQPLDR
GAGGAPPKDPGFRAIIQMGNLAGLPALVLPCGFAENMPVALQVVGVPFSENTLLAIGREFQKRTDWHKRHPA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=504, Percent_Identity=26.7857142857143, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=23.5412474849095, Blast_Score=102, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=419, Percent_Identity=27.2076372315036, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17537465, Length=479, Percent_Identity=23.5908141962422, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=312, Percent_Identity=29.1666666666667, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=309, Percent_Identity=27.5080906148867, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=334, Percent_Identity=24.8502994011976, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=334, Percent_Identity=24.8502994011976, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=439, Percent_Identity=25.0569476082005, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=422, Percent_Identity=24.1706161137441, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648113, Length=463, Percent_Identity=28.2937365010799, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24644968, Length=497, Percent_Identity=25.7545271629779, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=22.4899598393574, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=22.4899598393574, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=22.4899598393574, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=22.4899598393574, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356731, Length=487, Percent_Identity=25.2566735112936, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45550774, Length=478, Percent_Identity=22.8033472803347, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648435, Length=478, Percent_Identity=22.8033472803347, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24648437, Length=478, Percent_Identity=22.8033472803347, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=24.4, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50202; Mature: 50071

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIDPDVYFATIPELNERLKSKEFTAEQLARAFSARLQELGPRYNALALSLNQEALRQA
CCCCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
KAVDNELKRDRFRGPLQGIPYGVKDLLAYAGQPTTWGAKPYAGQVFDYNAAVIDKISALG
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHH
GVLTGKLAMVELAGGGGYRFASASLTGPGLNPWDRTRWSGGSSSGSAAAVAAGLVPYAIG
HHHHCCEEEEEEECCCCEEEEECCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCC
SETSGSIVTPASFCGVTALRPTYGLVSRYGAMALSWTLDKLGPFAHTAEDCGLILQAIAG
CCCCCCEECCHHHHCCHHCCHHHHHHHHHHHHHEEHHHHHHCCHHCCHHHHHHHHHHHHC
KDSRDPGSAGKSFYYTPQYAHPMKDLKVGYAPSDFSEWAEPAARPAFDAALGVLKASGVQ
CCCCCCCCCCCCEEECCCHHCCHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCH
LVETRLPDFPYGAALSTILNGEMGSIFEPLIASGKVDELADKPQIAGLKASLELPAKDYL
HHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCEEECCCCHHHHH
KAMRMRRMIQDEVSKLFGSIDVLLAPGRPGIASKIDQPLDRGAGGAPPKDPGFRAIIQMG
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
NLAGLPALVLPCGFAENMPVALQVVGVPFSENTLLAIGREFQKRTDWHKRHPA
CCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAIDPDVYFATIPELNERLKSKEFTAEQLARAFSARLQELGPRYNALALSLNQEALRQA
CCCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
KAVDNELKRDRFRGPLQGIPYGVKDLLAYAGQPTTWGAKPYAGQVFDYNAAVIDKISALG
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHH
GVLTGKLAMVELAGGGGYRFASASLTGPGLNPWDRTRWSGGSSSGSAAAVAAGLVPYAIG
HHHHCCEEEEEEECCCCEEEEECCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCC
SETSGSIVTPASFCGVTALRPTYGLVSRYGAMALSWTLDKLGPFAHTAEDCGLILQAIAG
CCCCCCEECCHHHHCCHHCCHHHHHHHHHHHHHEEHHHHHHCCHHCCHHHHHHHHHHHHC
KDSRDPGSAGKSFYYTPQYAHPMKDLKVGYAPSDFSEWAEPAARPAFDAALGVLKASGVQ
CCCCCCCCCCCCEEECCCHHCCHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCH
LVETRLPDFPYGAALSTILNGEMGSIFEPLIASGKVDELADKPQIAGLKASLELPAKDYL
HHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCEEECCCCHHHHH
KAMRMRRMIQDEVSKLFGSIDVLLAPGRPGIASKIDQPLDRGAGGAPPKDPGFRAIIQMG
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
NLAGLPALVLPCGFAENMPVALQVVGVPFSENTLLAIGREFQKRTDWHKRHPA
CCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA