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The map label for this gene is yjbJ [H]

Identifier: 116626178

GI number: 116626178

Start: 8958420

End: 8959052

Strand: Direct

Name: yjbJ [H]

Synonym: Acid_7138

Alternate gene names: 116626178

Gene position: 8958420-8959052 (Clockwise)

Preceding gene: 116626173

Following gene: 116626180

Centisome position: 89.89

GC content: 58.93

Gene sequence:

>633_bases
ATGAAATTCTCCCTAGTAACCCTAGCAGTGGCCAGCCTTGCGCTTGCCGGCTCCAGCTTTTTTGTGCTTTCTTCCCCAGG
GTCTTTGCCGGCGCGCGAAACTACGGCGATCGGCCAGATTGCGGCTCGCATCCCTGAGAAGCTTGCGCAGGTGAGAACCA
TCGACGTGCACGCCTTGATTCACGCGGCGGCGGCGAAACACAAAGTCCCCGAAGCCTTCGTGAAGAGCATCGTTGCAGCC
GAATCTAATTTCGATGCTGCGGCAGTGTCGCGCACCGGAGCGATTGGCCTGATGCAACTCATGCCGGCCACTGCGCAGGA
ATACAGCGCCGATCCGAAAATCCCGGAACAGAATATCGAAGCCGGAACCAAATACCTCCGCTGCCTGATGACCCGGTATC
AAAAGAGCCGGAACTCGCTGACCCGCGTAATTGCGGCCTACAATGCCGGTCCTACGGTCGTCGATCGGTATCGCGGAATA
CCGCCATACCGCGAAACGCGCAGGTATGTGGTCCGCGTGCTCGGATTCATGAAGCAATTCGAAAGGGAGCATGCGAAGGT
GGGCTGCGTGGAGCAGTTTGCTAAGAACGCGCCAGCCCCTGTCATGCAGGGCGGCACCAGCGACGGCGAATAG

Upstream 100 bases:

>100_bases
TTCCCGTACGCTCTTGCGCTCTCGCGGTTTGCTGCCTCAACCGTATTTCCGATGCAGTACTCTGATTTCGCAGTAACGTC
CTTTAATAGCTCACACAAAA

Downstream 100 bases:

>100_bases
ACTCGCGGGCCGGTAGTACCTCGAAGCCTCAAACGGCGTCGTCGGCTCAGTACCCCCAGGAACTGGAATTGGTACAGACC
GGGCGCTTCTTTGCGGCCGG

Product: lytic transglycosylase catalytic subunit subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKFSLVTLAVASLALAGSSFFVLSSPGSLPARETTAIGQIAARIPEKLAQVRTIDVHALIHAAAAKHKVPEAFVKSIVAA
ESNFDAAAVSRTGAIGLMQLMPATAQEYSADPKIPEQNIEAGTKYLRCLMTRYQKSRNSLTRVIAAYNAGPTVVDRYRGI
PPYRETRRYVVRVLGFMKQFEREHAKVGCVEQFAKNAPAPVMQGGTSDGE

Sequences:

>Translated_210_residues
MKFSLVTLAVASLALAGSSFFVLSSPGSLPARETTAIGQIAARIPEKLAQVRTIDVHALIHAAAAKHKVPEAFVKSIVAA
ESNFDAAAVSRTGAIGLMQLMPATAQEYSADPKIPEQNIEAGTKYLRCLMTRYQKSRNSLTRVIAAYNAGPTVVDRYRGI
PPYRETRRYVVRVLGFMKQFEREHAKVGCVEQFAKNAPAPVMQGGTSDGE
>Mature_210_residues
MKFSLVTLAVASLALAGSSFFVLSSPGSLPARETTAIGQIAARIPEKLAQVRTIDVHALIHAAAAKHKVPEAFVKSIVAA
ESNFDAAAVSRTGAIGLMQLMPATAQEYSADPKIPEQNIEAGTKYLRCLMTRYQKSRNSLTRVIAAYNAGPTVVDRYRGI
PPYRETRRYVVRVLGFMKQFEREHAKVGCVEQFAKNAPAPVMQGGTSDGE

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=161, Percent_Identity=30.4347826086957, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 22708; Mature: 22708

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSLVTLAVASLALAGSSFFVLSSPGSLPARETTAIGQIAARIPEKLAQVRTIDVHALI
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAAAAKHKVPEAFVKSIVAAESNFDAAAVSRTGAIGLMQLMPATAQEYSADPKIPEQNIE
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
AGTKYLRCLMTRYQKSRNSLTRVIAAYNAGPTVVDRYRGIPPYRETRRYVVRVLGFMKQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EREHAKVGCVEQFAKNAPAPVMQGGTSDGE
HHHHHHHHHHHHHHCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MKFSLVTLAVASLALAGSSFFVLSSPGSLPARETTAIGQIAARIPEKLAQVRTIDVHALI
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAAAAKHKVPEAFVKSIVAAESNFDAAAVSRTGAIGLMQLMPATAQEYSADPKIPEQNIE
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHH
AGTKYLRCLMTRYQKSRNSLTRVIAAYNAGPTVVDRYRGIPPYRETRRYVVRVLGFMKQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EREHAKVGCVEQFAKNAPAPVMQGGTSDGE
HHHHHHHHHHHHHHCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]