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The map label for this gene is yitJ [H]

Identifier: 116626155

GI number: 116626155

Start: 8932747

End: 8934615

Strand: Direct

Name: yitJ [H]

Synonym: Acid_7115

Alternate gene names: 116626155

Gene position: 8932747-8934615 (Clockwise)

Preceding gene: 116626154

Following gene: 116626156

Centisome position: 89.64

GC content: 63.94

Gene sequence:

>1869_bases
ATGAATACAACCACCAGTAGCCGCGCCCAGGAATTTCGTGACCAGCTTGCCCGCCGTGTGATCGTGGCCGATGGAGCGAT
GGGTACCATGCTCTATTCGCGCGGTGTGTTTATCAACCGCTGCTTCGATGAGCTGAACCTCTCCGCGGCCGACATGGTCC
GGCAGATTCACCAGGAGTACGTCAAGGCCGGCGCGGAGATTCTGGAGACCAATACCTTCGGCGCCACGCGGCAGCGGCTC
GGTGCCTTCGGGTTCGCCGAAAAACTCCAGGCTATCAATCACGCGGGTGTCCGCCTGGCGCGCGAAGCTGCCAACGGGAG
CGCCTACGTCGCGGGCGCCGTCGGTCCCCTGAACGTGCGCATCGAGCCGCTCGGGCCTACCTCCTTCGCCGAGGCGCGCG
CGGCCTTTCGCGAACAGATCGACGCGCTGCTCGAAGCAGGCGTGGACCTGCTGATCTTCGAAACCTTCGGCAACCTCGAT
GAATTGCGCGAGGCCGTCTACGCCGGCTACGACGCGTCGCGCGGTGAAGTGCCCATGATCGCGCAGGTGACGATCGACGA
TTTCGGCCACCTGCCGGGCGGCACCGATACGGAGACGTTCACGCGCGAAATGAACAATTGGCCGGTCGATGTGATCGGGC
TGAACTGCTCCGTGGGCCCCAAGGCAACGCTCGAGACGGTGGAGCGCATGATGGAATTCGCCACCAAGCCGCTGAGCGCC
ATGCCCAATGCGGGTCTCCCGATGCGGGTCGAAGGACGCAACATCTATTTGTGTTCGCCCGAGTACATGTCGCAGTACGC
ACGCCGCCTTTTGTGGGCCGGTGTAAAGGTGATCGGCGGCTGCTGTGGCACCACGCCAGACCATATCAAGTTGATCCGCA
GCGAGACTCGCTCCCTGCAGCCGCTGCAGAAGCAGTTGGCCGTCACGGTGGAAGAGCCCAAGGTGAAGGCGCAGGCCCTG
CAGCCGGTACCGGTGGCCGAAAAGTCGCAGGTAGGGGCCAAGCTCGCGGCCGGCAAGTTCGTCACCTTCGTCGAAATCCT
GCCGCCGCGCGGCGTGGACGCCACGCGCGAGATCGCCGGCGCGAAGCTCTGCGCGGAGCACGGCATCGACGCCATTAACG
TGCCGGATGGGCCGCGTGCCAGCGCGCGCATGAGCGCCCAGGTCACCTGCCAGTTGATTCAGGCGCAGGCTGGAATCGAG
GCCGTAAACCATTTCTGTTGCCGCGACCGCAATATCCTCGGCATCCAGAGCGAGCTGCTCGGGACTCACGGAGCCGGCGT
ACGCAACCTCATCTGTATCACGGGCGATCCGCCCCGCATGGGTGCCTATCCCGACGCCACGGCGGTCTTCGACGTTGACG
CCATCGGCCTGGTGAACATCGTTCGCAATCTCAATCACGGGCTCGATATCGGCGGCAATCCGATGGGCTCGCAGACCGCG
CTGCTGATCGGCGTAGGAGCCAACCCTGGCGCGTTGAATATGGATGAGGAGATCCGCCGCTTCGAGTGGAAGGTGGAAGC
CGGCGCCGAGTACGTGGTCACGCAGCCGGTCTTCGACCTGGACCTGCTCGAAGCCTTCCTCAAGCGCATCGAGCACGTCA
AAATTCCCGTGCTGTGCGGCATCTGGCCGCTTACCAGCTACCGCAACGCCGAGTTCATGGTCAATGAGCTGCGCGTACCG
GTGCCGGAGATGTTCATGGAGCGCATGCGCCGCGTGGATAACGCCGAAAAGGCGCGCGAGGAAGGTGTGGCGATCGCGCG
CGAAATGGTTGCGCGGGTCAGCAAAATGGTGCAGGGCGCGCAACTCAGCGCGCCTTTCGGCCGCTATCAGATGGCGATCG
ACGTTGCCGATGCCATCGAGTCCCGCTAA

Upstream 100 bases:

>100_bases
ACGTGATCCTCGGCAGATAGCCACCCCCCTTGCCACGTGCGCTAAAATCGGGAATATCGCATTTCCCGCTCGCTTTACCG
GTACGTGCGACCGCTATCTC

Downstream 100 bases:

>100_bases
GATCGGAACACAAGATCCGAAACATGGCAACCGACCTGGTCGAAATCAATACCGAAGATCCCCAGCCGGAAGCGCTGGAG
AGGGCCGCCGCCGCTGTGCG

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNTTTSSRAQEFRDQLARRVIVADGAMGTMLYSRGVFINRCFDELNLSAADMVRQIHQEYVKAGAEILETNTFGATRQRL
GAFGFAEKLQAINHAGVRLAREAANGSAYVAGAVGPLNVRIEPLGPTSFAEARAAFREQIDALLEAGVDLLIFETFGNLD
ELREAVYAGYDASRGEVPMIAQVTIDDFGHLPGGTDTETFTREMNNWPVDVIGLNCSVGPKATLETVERMMEFATKPLSA
MPNAGLPMRVEGRNIYLCSPEYMSQYARRLLWAGVKVIGGCCGTTPDHIKLIRSETRSLQPLQKQLAVTVEEPKVKAQAL
QPVPVAEKSQVGAKLAAGKFVTFVEILPPRGVDATREIAGAKLCAEHGIDAINVPDGPRASARMSAQVTCQLIQAQAGIE
AVNHFCCRDRNILGIQSELLGTHGAGVRNLICITGDPPRMGAYPDATAVFDVDAIGLVNIVRNLNHGLDIGGNPMGSQTA
LLIGVGANPGALNMDEEIRRFEWKVEAGAEYVVTQPVFDLDLLEAFLKRIEHVKIPVLCGIWPLTSYRNAEFMVNELRVP
VPEMFMERMRRVDNAEKAREEGVAIAREMVARVSKMVQGAQLSAPFGRYQMAIDVADAIESR

Sequences:

>Translated_622_residues
MNTTTSSRAQEFRDQLARRVIVADGAMGTMLYSRGVFINRCFDELNLSAADMVRQIHQEYVKAGAEILETNTFGATRQRL
GAFGFAEKLQAINHAGVRLAREAANGSAYVAGAVGPLNVRIEPLGPTSFAEARAAFREQIDALLEAGVDLLIFETFGNLD
ELREAVYAGYDASRGEVPMIAQVTIDDFGHLPGGTDTETFTREMNNWPVDVIGLNCSVGPKATLETVERMMEFATKPLSA
MPNAGLPMRVEGRNIYLCSPEYMSQYARRLLWAGVKVIGGCCGTTPDHIKLIRSETRSLQPLQKQLAVTVEEPKVKAQAL
QPVPVAEKSQVGAKLAAGKFVTFVEILPPRGVDATREIAGAKLCAEHGIDAINVPDGPRASARMSAQVTCQLIQAQAGIE
AVNHFCCRDRNILGIQSELLGTHGAGVRNLICITGDPPRMGAYPDATAVFDVDAIGLVNIVRNLNHGLDIGGNPMGSQTA
LLIGVGANPGALNMDEEIRRFEWKVEAGAEYVVTQPVFDLDLLEAFLKRIEHVKIPVLCGIWPLTSYRNAEFMVNELRVP
VPEMFMERMRRVDNAEKAREEGVAIAREMVARVSKMVQGAQLSAPFGRYQMAIDVADAIESR
>Mature_622_residues
MNTTTSSRAQEFRDQLARRVIVADGAMGTMLYSRGVFINRCFDELNLSAADMVRQIHQEYVKAGAEILETNTFGATRQRL
GAFGFAEKLQAINHAGVRLAREAANGSAYVAGAVGPLNVRIEPLGPTSFAEARAAFREQIDALLEAGVDLLIFETFGNLD
ELREAVYAGYDASRGEVPMIAQVTIDDFGHLPGGTDTETFTREMNNWPVDVIGLNCSVGPKATLETVERMMEFATKPLSA
MPNAGLPMRVEGRNIYLCSPEYMSQYARRLLWAGVKVIGGCCGTTPDHIKLIRSETRSLQPLQKQLAVTVEEPKVKAQAL
QPVPVAEKSQVGAKLAAGKFVTFVEILPPRGVDATREIAGAKLCAEHGIDAINVPDGPRASARMSAQVTCQLIQAQAGIE
AVNHFCCRDRNILGIQSELLGTHGAGVRNLICITGDPPRMGAYPDATAVFDVDAIGLVNIVRNLNHGLDIGGNPMGSQTA
LLIGVGANPGALNMDEEIRRFEWKVEAGAEYVVTQPVFDLDLLEAFLKRIEHVKIPVLCGIWPLTSYRNAEFMVNELRVP
VPEMFMERMRRVDNAEKAREEGVAIAREMVARVSKMVQGAQLSAPFGRYQMAIDVADAIESR

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=473, Percent_Identity=27.2727272727273, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI13162290, Length=295, Percent_Identity=29.1525423728814, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI157266337, Length=302, Percent_Identity=29.8013245033113, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1790450, Length=361, Percent_Identity=31.5789473684211, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1786456, Length=301, Percent_Identity=28.9036544850498, Blast_Score=92, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17535405, Length=477, Percent_Identity=27.0440251572327, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324982, Length=320, Percent_Identity=24.375, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322966, Length=305, Percent_Identity=23.6065573770492, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 67728; Mature: 67728

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTTSSRAQEFRDQLARRVIVADGAMGTMLYSRGVFINRCFDELNLSAADMVRQIHQEY
CCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
VKAGAEILETNTFGATRQRLGAFGFAEKLQAINHAGVRLAREAANGSAYVAGAVGPLNVR
HHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEE
IEPLGPTSFAEARAAFREQIDALLEAGVDLLIFETFGNLDELREAVYAGYDASRGEVPMI
EEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHCCCCCCCCCCCEE
AQVTIDDFGHLPGGTDTETFTREMNNWPVDVIGLNCSVGPKATLETVERMMEFATKPLSA
EEEEEHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHH
MPNAGLPMRVEGRNIYLCSPEYMSQYARRLLWAGVKVIGGCCGTTPDHIKLIRSETRSLQ
CCCCCCEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
PLQKQLAVTVEEPKVKAQALQPVPVAEKSQVGAKLAAGKFVTFVEILPPRGVDATREIAG
HHHHHHEEEECCCCCHHHCCCCCCCCCHHHCCHHHHCCCCEEEEEECCCCCCCHHHHHHH
AKLCAEHGIDAINVPDGPRASARMSAQVTCQLIQAQAGIEAVNHFCCRDRNILGIQSELL
HHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHH
GTHGAGVRNLICITGDPPRMGAYPDATAVFDVDAIGLVNIVRNLNHGLDIGGNPMGSQTA
CCCCCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCEE
LLIGVGANPGALNMDEEIRRFEWKVEAGAEYVVTQPVFDLDLLEAFLKRIEHVKIPVLCG
EEEEECCCCCCCCCHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEC
IWPLTSYRNAEFMVNELRVPVPEMFMERMRRVDNAEKAREEGVAIAREMVARVSKMVQGA
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
QLSAPFGRYQMAIDVADAIESR
CCCCCCCCEEEEEEHHHHHCCC
>Mature Secondary Structure
MNTTTSSRAQEFRDQLARRVIVADGAMGTMLYSRGVFINRCFDELNLSAADMVRQIHQEY
CCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHH
VKAGAEILETNTFGATRQRLGAFGFAEKLQAINHAGVRLAREAANGSAYVAGAVGPLNVR
HHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEE
IEPLGPTSFAEARAAFREQIDALLEAGVDLLIFETFGNLDELREAVYAGYDASRGEVPMI
EEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHHHHCCCCCCCCCCCEE
AQVTIDDFGHLPGGTDTETFTREMNNWPVDVIGLNCSVGPKATLETVERMMEFATKPLSA
EEEEEHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHH
MPNAGLPMRVEGRNIYLCSPEYMSQYARRLLWAGVKVIGGCCGTTPDHIKLIRSETRSLQ
CCCCCCEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
PLQKQLAVTVEEPKVKAQALQPVPVAEKSQVGAKLAAGKFVTFVEILPPRGVDATREIAG
HHHHHHEEEECCCCCHHHCCCCCCCCCHHHCCHHHHCCCCEEEEEECCCCCCCHHHHHHH
AKLCAEHGIDAINVPDGPRASARMSAQVTCQLIQAQAGIEAVNHFCCRDRNILGIQSELL
HHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHH
GTHGAGVRNLICITGDPPRMGAYPDATAVFDVDAIGLVNIVRNLNHGLDIGGNPMGSQTA
CCCCCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCEE
LLIGVGANPGALNMDEEIRRFEWKVEAGAEYVVTQPVFDLDLLEAFLKRIEHVKIPVLCG
EEEEECCCCCCCCCHHHHHHHHEEECCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEC
IWPLTSYRNAEFMVNELRVPVPEMFMERMRRVDNAEKAREEGVAIAREMVARVSKMVQGA
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
QLSAPFGRYQMAIDVADAIESR
CCCCCCCCEEEEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]