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The map label for this gene is tilS

Identifier: 116626117

GI number: 116626117

Start: 8891384

End: 8892706

Strand: Reverse

Name: tilS

Synonym: Acid_7075

Alternate gene names: 116626117

Gene position: 8892706-8891384 (Counterclockwise)

Preceding gene: 116626118

Following gene: 116626116

Centisome position: 89.23

GC content: 63.11

Gene sequence:

>1323_bases
ATGTTCGCGAGCGGGGCGCGTGTGGCGGTGGGCGTCTCGGGCGGCGCGGACTCGGTGTGCCTGCTGCACGCTCTGGTGGA
ACTCGGCGGACTCAAACTCAGCGTGCTGCATGTGGATCACGGACTGCGCGGCGCGGAATCGCGGGCCGACGCGGAGTTCG
TTGGGGAACTGGCGGCGCGGATGGGACTCCCGTTTTGCCTGCGCGAGGTAACGCTTGGGCCGGGCAATGTCGAGCAGGAA
GGGCGCAGGGCGCGGCTCAGGTTCTTTCACGAGCAGCTCGCGGCGGGAAATTGCGACCGCGTGGCGCTGGGTCATACGCG
CAGCGATCAGGCGGAGACGGTGTTGTTCCGGTTCCTGCGCGGCAGCGGGACCGCGGGGCTGGCCGGGATCCGACCGGTGA
CTGCCGAAGGAATCGTGCGGCCGCTGATCGAACTGGAACGCGCGGAAATCGAGGAGTATTTGCGTGAGCGCCAGATTCCG
TGGCGGGAGGATGCGACCAACGCCGGCGAGGAGTTCGCACGCAACCGGATTCGTCACGGATTGCTGCCGCAACTCGCGGC
GGAGTGGAATCCGGCGTTGGTGGAAACCCTGGCGCACACTGCGGAGTTCGCGCGGGCGGAAGAGGCGTATTGGGCCGGGG
AGATCGACAGGTTGTCGGCAGAGAGCCTGACGGCTGGTGACGGATGCGTGCTAATCCGCACGGAGTCTTTGAAAGCGCTG
TCACTGGCAGTAGCTCGCCGGCTGGTTCGGCGTGCCATTCAGATGGCGAAGGGCGACCTGCGCGGAGTCGATTTTGGCCA
CGTCGAGCGGGTGGTGGAACTCGCGTCCGCCCCCACCGGTAGAGGGCGAACGCAAGTTCCCGGGCTGGACGTACGCCGCT
CCTTTGAATGGCTTAGATTGGGCGTGCCGTTTACCCGCAAGCCATACAGTCTTAAACCTCTTGTACCCGGAACTACGCAG
ATCCCGGGAACCCGCAACGGTATTTCCCTGGAACTAATTGAAAAATCGGAAACTTCCGTGTTGCCATGGAACGTATATAA
TACTGAGATGGGTTGTCTCGACTGGAAGCGGCTGGCCGGTTCCCTCGAACTTCGGAACTGGCGCTTCGGTGATCAGTATC
AACCCATGGGGCTTACGGGCTCAGAAAAGATCAAGACCCTGTTCCAACTCCAACGTATTCCTGTGTGGGAACGTGCGCAG
TGGCCGGTTCTTACGGATGGGGAGTCCATTGTGTGGACTCGCCGGTTCGGTCCGGCCGCGGGCTTTGCGGCGGGTCCGGA
AAGCGGCGTCGTTCTCAAACTCGGAGAGGTGACAATCCGTTGA

Upstream 100 bases:

>100_bases
ACGCAATGGGACAAGCTGATCGCGGCGTACGAGAAGGGAATGAGCGCGGCGGTGCGCGAATTTGATTGAGCGGGTACGAC
GGACGATCGAGCGGTACGGA

Downstream 100 bases:

>100_bases
CGATTGCAGAATCGGGAAGGCTGGGGGCGGCGTCTAAAGAACTGACGGCCCGTACGGAGGTTTCGTGAATTCAAACATAA
AGACATTGATTTTCTGGGTA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MFASGARVAVGVSGGADSVCLLHALVELGGLKLSVLHVDHGLRGAESRADAEFVGELAARMGLPFCLREVTLGPGNVEQE
GRRARLRFFHEQLAAGNCDRVALGHTRSDQAETVLFRFLRGSGTAGLAGIRPVTAEGIVRPLIELERAEIEEYLRERQIP
WREDATNAGEEFARNRIRHGLLPQLAAEWNPALVETLAHTAEFARAEEAYWAGEIDRLSAESLTAGDGCVLIRTESLKAL
SLAVARRLVRRAIQMAKGDLRGVDFGHVERVVELASAPTGRGRTQVPGLDVRRSFEWLRLGVPFTRKPYSLKPLVPGTTQ
IPGTRNGISLELIEKSETSVLPWNVYNTEMGCLDWKRLAGSLELRNWRFGDQYQPMGLTGSEKIKTLFQLQRIPVWERAQ
WPVLTDGESIVWTRRFGPAAGFAAGPESGVVLKLGEVTIR

Sequences:

>Translated_440_residues
MFASGARVAVGVSGGADSVCLLHALVELGGLKLSVLHVDHGLRGAESRADAEFVGELAARMGLPFCLREVTLGPGNVEQE
GRRARLRFFHEQLAAGNCDRVALGHTRSDQAETVLFRFLRGSGTAGLAGIRPVTAEGIVRPLIELERAEIEEYLRERQIP
WREDATNAGEEFARNRIRHGLLPQLAAEWNPALVETLAHTAEFARAEEAYWAGEIDRLSAESLTAGDGCVLIRTESLKAL
SLAVARRLVRRAIQMAKGDLRGVDFGHVERVVELASAPTGRGRTQVPGLDVRRSFEWLRLGVPFTRKPYSLKPLVPGTTQ
IPGTRNGISLELIEKSETSVLPWNVYNTEMGCLDWKRLAGSLELRNWRFGDQYQPMGLTGSEKIKTLFQLQRIPVWERAQ
WPVLTDGESIVWTRRFGPAAGFAAGPESGVVLKLGEVTIR
>Mature_440_residues
MFASGARVAVGVSGGADSVCLLHALVELGGLKLSVLHVDHGLRGAESRADAEFVGELAARMGLPFCLREVTLGPGNVEQE
GRRARLRFFHEQLAAGNCDRVALGHTRSDQAETVLFRFLRGSGTAGLAGIRPVTAEGIVRPLIELERAEIEEYLRERQIP
WREDATNAGEEFARNRIRHGLLPQLAAEWNPALVETLAHTAEFARAEEAYWAGEIDRLSAESLTAGDGCVLIRTESLKAL
SLAVARRLVRRAIQMAKGDLRGVDFGHVERVVELASAPTGRGRTQVPGLDVRRSFEWLRLGVPFTRKPYSLKPLVPGTTQ
IPGTRNGISLELIEKSETSVLPWNVYNTEMGCLDWKRLAGSLELRNWRFGDQYQPMGLTGSEKIKTLFQLQRIPVWERAQ
WPVLTDGESIVWTRRFGPAAGFAAGPESGVVLKLGEVTIR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=212, Percent_Identity=30.6603773584906, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_SOLUE (Q01QT2)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_828273.1
- ProteinModelPortal:   Q01QT2
- SMR:   Q01QT2
- STRING:   Q01QT2
- GeneID:   4428972
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_7075
- NMPDR:   fig|234267.9.peg.6705
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   YLRTRIR
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 48424; Mature: 48424

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASGARVAVGVSGGADSVCLLHALVELGGLKLSVLHVDHGLRGAESRADAEFVGELAAR
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
MGLPFCLREVTLGPGNVEQEGRRARLRFFHEQLAAGNCDRVALGHTRSDQAETVLFRFLR
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
GSGTAGLAGIRPVTAEGIVRPLIELERAEIEEYLRERQIPWREDATNAGEEFARNRIRHG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
LLPQLAAEWNPALVETLAHTAEFARAEEAYWAGEIDRLSAESLTAGDGCVLIRTESLKAL
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEECCHHHH
SLAVARRLVRRAIQMAKGDLRGVDFGHVERVVELASAPTGRGRTQVPGLDVRRSFEWLRL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHEE
GVPFTRKPYSLKPLVPGTTQIPGTRNGISLELIEKSETSVLPWNVYNTEMGCLDWKRLAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECEEECCCCCCCHHHHHHCC
SLELRNWRFGDQYQPMGLTGSEKIKTLFQLQRIPVWERAQWPVLTDGESIVWTRRFGPAA
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCC
GFAAGPESGVVLKLGEVTIR
CCCCCCCCCEEEEECCEEEC
>Mature Secondary Structure
MFASGARVAVGVSGGADSVCLLHALVELGGLKLSVLHVDHGLRGAESRADAEFVGELAAR
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHH
MGLPFCLREVTLGPGNVEQEGRRARLRFFHEQLAAGNCDRVALGHTRSDQAETVLFRFLR
CCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
GSGTAGLAGIRPVTAEGIVRPLIELERAEIEEYLRERQIPWREDATNAGEEFARNRIRHG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
LLPQLAAEWNPALVETLAHTAEFARAEEAYWAGEIDRLSAESLTAGDGCVLIRTESLKAL
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEECCHHHH
SLAVARRLVRRAIQMAKGDLRGVDFGHVERVVELASAPTGRGRTQVPGLDVRRSFEWLRL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHEE
GVPFTRKPYSLKPLVPGTTQIPGTRNGISLELIEKSETSVLPWNVYNTEMGCLDWKRLAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECEEECCCCCCCHHHHHHCC
SLELRNWRFGDQYQPMGLTGSEKIKTLFQLQRIPVWERAQWPVLTDGESIVWTRRFGPAA
CEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCC
GFAAGPESGVVLKLGEVTIR
CCCCCCCCCEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA