Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 116625991

GI number: 116625991

Start: 8731764

End: 8733305

Strand: Reverse

Name: oprM [H]

Synonym: Acid_6949

Alternate gene names: 116625991

Gene position: 8733305-8731764 (Counterclockwise)

Preceding gene: 116625992

Following gene: 116625990

Centisome position: 87.63

GC content: 60.51

Gene sequence:

>1542_bases
ATGAGGGACGGTAATACAAAGGTACGTTGGGTGTCCGGGGCGGCAGCGCTTTTCCTGTCTGCCGGCTGCACGGTCGGCCC
GAAATACCACCCGCCGGCCGCGACACAGGCTCCGGTCCCCACGGTGTACAAGGAATCCCCCACGCAGTTCAAGGAGACCG
AGGGCTGGAAGGTGGCTCGCCCAGGAGATGCGATGTTGCGCGGCAAGTGGTGGGAAATCTTCAAGGAACCCGAGTTGAAC
AGCCTCGAAGATCGCCTCAACATCGATAACGAGAATATCAAGCAGGCCTTCGAGAACTTCATGGCTGCTCGCGCACTCAT
CCGGGAAGCCCGGGCCCAGTACTTTCCCACATTGACTACCACGCCATCCTTCAGCCGGTCGGCCTCCTCGGCGAACGTGG
GCGGTGTGACGGTGGCAAATGGAAGCAGCGGAGTGGCCGCGGTCTCCGGCCGGCAACTCACCCTCTATTCCGTGCCGCTT
GACATCTCATGGGCACCCGACCTTTGGGGCAGGGTGAGAAACACAGTGCGCGAATTCCAAGCCGGCGCCCAGTTGAGCGC
TGCCGATCTGGAGAATGAACGCCTGACCGAACAGGCCAGCCTGGCCCAATTCTATTTCGAGATCCGGGGACAGGATGCGC
TCCAGAAGATCTTAAACGAGACGGTGGAAGCCGATAAGAAGGCGCTCGAATTGACACAGGCTCGTTACCAGACTGGCGTC
GGCGATCAGCTTGACGTGGTCCAGGCACAGACTACCTTGGAGAGCGTGCAGGCACAGGCGATCTCTATCGGTCTTTTGCG
AGCTCAGTTCGAACATGCGATCGCCACGCTGATCGGCAGGCCGGCGTCAAGCTTTTCCATCCCGGTGAAGCCGATGACCA
CCGCTCCGCCGCCCATCCCGATCGGCATGCCTTCGCAACTGCTGGAGCGGCGTCCGGATATCGCGGCAGCGGAACGCAAT
ATGGCGGTAGCCAATGCCCAAATAGGCATAGCCTATGCGGCTTTCTATCCCACACTGACCCTCTCCGCGACAGGCGGCGC
CGAAAGCTCGACGCTCGAGAGACTCTTCACCTGGTCCAGCCGGGTATGGTCAATCGGCGGGAGCGTCTCAGAGACGATCT
TCGATGCCGGACTCCGCCGCGCCACGGTAAACCAGTTCATCGCGACCTACAATGCGGCGGTGGCCGGGTACCGGCAGACT
GTGCTCACCGCCTTTCAGCAGGTGGAGGATTCGCTTTCAGGAGTGCGCATTCTCTCCCAGGAAGTGGTGAAGCAAAAGCA
GGCGGTCCAATCCGCGGAGACTCAACTGACGCTCCAGACCGCGCGCTATGAAACCGGAATCGATCCTTACCTCAATGTCG
TGATCGCCCAAACCACCCTGCTCAGCAACCGGCAAAACCTGGTCACTGCCCAGATATCAGAGATGACCTCCGCTGTTCAG
CTGATCCTGGCCTTGGGCGGAGGTTGGGACCGGTCCCAACTCCCCACCCCCGAGCAGGTAAGCGAAAAACCTACCAAGTC
TGAAACCACGATCCGCCACTAG

Upstream 100 bases:

>100_bases
CCCGTGGTCTATTTATTTCTTGACGGTTTGCGCGTTCGTTTCCAGAGGCGCAATGAGGGATTGGTGGGCCCGACGGAAGC
CCCTTCGTAGGTCGATACAC

Downstream 100 bases:

>100_bases
CAGCCCTCCTTCTTCGCCCTAATAGGAATTTCTAGGAATTCCGGCTGCAATTCTGGCGCATTAGAAATAAATATGAGAAG
GTACTAGTACCATTCTCACA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MRDGNTKVRWVSGAAALFLSAGCTVGPKYHPPAATQAPVPTVYKESPTQFKETEGWKVARPGDAMLRGKWWEIFKEPELN
SLEDRLNIDNENIKQAFENFMAARALIREARAQYFPTLTTTPSFSRSASSANVGGVTVANGSSGVAAVSGRQLTLYSVPL
DISWAPDLWGRVRNTVREFQAGAQLSAADLENERLTEQASLAQFYFEIRGQDALQKILNETVEADKKALELTQARYQTGV
GDQLDVVQAQTTLESVQAQAISIGLLRAQFEHAIATLIGRPASSFSIPVKPMTTAPPPIPIGMPSQLLERRPDIAAAERN
MAVANAQIGIAYAAFYPTLTLSATGGAESSTLERLFTWSSRVWSIGGSVSETIFDAGLRRATVNQFIATYNAAVAGYRQT
VLTAFQQVEDSLSGVRILSQEVVKQKQAVQSAETQLTLQTARYETGIDPYLNVVIAQTTLLSNRQNLVTAQISEMTSAVQ
LILALGGGWDRSQLPTPEQVSEKPTKSETTIRH

Sequences:

>Translated_513_residues
MRDGNTKVRWVSGAAALFLSAGCTVGPKYHPPAATQAPVPTVYKESPTQFKETEGWKVARPGDAMLRGKWWEIFKEPELN
SLEDRLNIDNENIKQAFENFMAARALIREARAQYFPTLTTTPSFSRSASSANVGGVTVANGSSGVAAVSGRQLTLYSVPL
DISWAPDLWGRVRNTVREFQAGAQLSAADLENERLTEQASLAQFYFEIRGQDALQKILNETVEADKKALELTQARYQTGV
GDQLDVVQAQTTLESVQAQAISIGLLRAQFEHAIATLIGRPASSFSIPVKPMTTAPPPIPIGMPSQLLERRPDIAAAERN
MAVANAQIGIAYAAFYPTLTLSATGGAESSTLERLFTWSSRVWSIGGSVSETIFDAGLRRATVNQFIATYNAAVAGYRQT
VLTAFQQVEDSLSGVRILSQEVVKQKQAVQSAETQLTLQTARYETGIDPYLNVVIAQTTLLSNRQNLVTAQISEMTSAVQ
LILALGGGWDRSQLPTPEQVSEKPTKSETTIRH
>Mature_513_residues
MRDGNTKVRWVSGAAALFLSAGCTVGPKYHPPAATQAPVPTVYKESPTQFKETEGWKVARPGDAMLRGKWWEIFKEPELN
SLEDRLNIDNENIKQAFENFMAARALIREARAQYFPTLTTTPSFSRSASSANVGGVTVANGSSGVAAVSGRQLTLYSVPL
DISWAPDLWGRVRNTVREFQAGAQLSAADLENERLTEQASLAQFYFEIRGQDALQKILNETVEADKKALELTQARYQTGV
GDQLDVVQAQTTLESVQAQAISIGLLRAQFEHAIATLIGRPASSFSIPVKPMTTAPPPIPIGMPSQLLERRPDIAAAERN
MAVANAQIGIAYAAFYPTLTLSATGGAESSTLERLFTWSSRVWSIGGSVSETIFDAGLRRATVNQFIATYNAAVAGYRQT
VLTAFQQVEDSLSGVRILSQEVVKQKQAVQSAETQLTLQTARYETGIDPYLNVVIAQTTLLSNRQNLVTAQISEMTSAVQ
LILALGGGWDRSQLPTPEQVSEKPTKSETTIRH

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=483, Percent_Identity=27.9503105590062, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI2367348, Length=486, Percent_Identity=23.8683127572016, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55812; Mature: 55812

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDGNTKVRWVSGAAALFLSAGCTVGPKYHPPAATQAPVPTVYKESPTQFKETEGWKVAR
CCCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEC
PGDAMLRGKWWEIFKEPELNSLEDRLNIDNENIKQAFENFMAARALIREARAQYFPTLTT
CCCHHHCCHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TPSFSRSASSANVGGVTVANGSSGVAAVSGRQLTLYSVPLDISWAPDLWGRVRNTVREFQ
CCCCCCCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AGAQLSAADLENERLTEQASLAQFYFEIRGQDALQKILNETVEADKKALELTQARYQTGV
CCCCCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDQLDVVQAQTTLESVQAQAISIGLLRAQFEHAIATLIGRPASSFSIPVKPMTTAPPPIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
IGMPSQLLERRPDIAAAERNMAVANAQIGIAYAAFYPTLTLSATGGAESSTLERLFTWSS
CCCCHHHHHCCCCCHHHHCCCEEECCHHEEEEEECCCEEEEEECCCCCHHHHHHHHHHHH
RVWSIGGSVSETIFDAGLRRATVNQFIATYNAAVAGYRQTVLTAFQQVEDSLSGVRILSQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVKQKQAVQSAETQLTLQTARYETGIDPYLNVVIAQTTLLSNRQNLVTAQISEMTSAVQ
HHHHHHHHHHHHHHHEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LILALGGGWDRSQLPTPEQVSEKPTKSETTIRH
HHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRDGNTKVRWVSGAAALFLSAGCTVGPKYHPPAATQAPVPTVYKESPTQFKETEGWKVAR
CCCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEC
PGDAMLRGKWWEIFKEPELNSLEDRLNIDNENIKQAFENFMAARALIREARAQYFPTLTT
CCCHHHCCHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TPSFSRSASSANVGGVTVANGSSGVAAVSGRQLTLYSVPLDISWAPDLWGRVRNTVREFQ
CCCCCCCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AGAQLSAADLENERLTEQASLAQFYFEIRGQDALQKILNETVEADKKALELTQARYQTGV
CCCCCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDQLDVVQAQTTLESVQAQAISIGLLRAQFEHAIATLIGRPASSFSIPVKPMTTAPPPIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
IGMPSQLLERRPDIAAAERNMAVANAQIGIAYAAFYPTLTLSATGGAESSTLERLFTWSS
CCCCHHHHHCCCCCHHHHCCCEEECCHHEEEEEECCCEEEEEECCCCCHHHHHHHHHHHH
RVWSIGGSVSETIFDAGLRRATVNQFIATYNAAVAGYRQTVLTAFQQVEDSLSGVRILSQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVVKQKQAVQSAETQLTLQTARYETGIDPYLNVVIAQTTLLSNRQNLVTAQISEMTSAVQ
HHHHHHHHHHHHHHHEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LILALGGGWDRSQLPTPEQVSEKPTKSETTIRH
HHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]