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The map label for this gene is hupE [H]

Identifier: 116625972

GI number: 116625972

Start: 8702128

End: 8703165

Strand: Direct

Name: hupE [H]

Synonym: Acid_6930

Alternate gene names: 116625972

Gene position: 8702128-8703165 (Clockwise)

Preceding gene: 116625971

Following gene: 116625973

Centisome position: 87.32

GC content: 63.1

Gene sequence:

>1038_bases
ATGAACTTCACTTGTCCGACTCCCCTCACGGGCTCCGACCGGATTCTTCTCGGCCACGGCAGCGGCGGCACCCTCAGTGC
CGCGTTGCTGAGCGAGATCGTTCTGCCTGCGTACAACAACACCATATTGGAGCGCATGGAAGATCAGGCTACGCTCGATA
TCAACGGCATAACTCTGGCGTTCACAACCGACTCGTTCGTAGTCAAACCAATCTTCTTCCCAGGCGGTGATATTGGCAGC
CTCGCCGTGCATGGGACCATCAACGATCTCGCCATGGGTGGTGCGCGGCCCGTTGCGCTTTCCGTCGCCATGATACTGGA
AGAGGGCTTCGCCGTATCGGACCTGAAGCGTATCGCCGGCAGCATCGGGGTCGCAGCCCGCGCGTGCGGCGTCCCCATCG
TCACCGGCGATACCAAGGTCGTCGGTCGAGGCTCGGGCGATGGGATTTTTCTCAACACCACCGGGATCGGCATCGTGCCG
CGCGGGTTGCAGCTTTCCGCCGCCAACGCGCGGCCTGGGGACATAGTGATCCTCAGCGGATCCATCGGCGATCACGGCAT
CGCGATTCTCGCCCAACGCGAGGGGCTCTCGCTCGATAGCGCGATCGAGAGCGATTCGGCCCCGCTGCATACACTCGCCG
CCTCTATTCTCAACGTGACCTTCGAGGTACGTTGTATGCGCGATCCCACCCGAGGCGGCCTCTCCAGCACGCTGAACGAG
ATCGCGCGCCAGTCCGGTGTGGGAATTACGATCCAAGAAACCGCTGTGCCGGTGCGTGAGGACGTACGCGGCGTCTGCGA
GCTCCTCGGCTTGGATCCGCTCTACGTCGCAAACGAGGGCAAGATGGTCGCGATCGTTCCGCCGCAGTCCGCCGCGGCGG
TGCTGGCCGCGATGCGCGCGCATCCGCTGGCCCGCGACGCCGCGATTATCGGCGAAGTCACGGAAGGTGAGTCAGGACTG
GTTTTGCAGCGGACCGCTTTCGGCACCCACCGCATCGTTACGATGTTGGCCGGCGATCAATTGCCGAGGATCTGCTAA

Upstream 100 bases:

>100_bases
CCTTCGGGAAGCAGTGTACACCGCAGGCGCCGCTCGGCGCACCCATGGTTTCGAGCGAAGGCGCGTGTGCCGCGTGGTAC
CAGTTCAAAGGTAGAGCATC

Downstream 100 bases:

>100_bases
TGCACGAACTCGGCATTGCGCACAGCGTATTGAAAGCCGCCCATCGCGAGGCGGAGCGGTTTCCGGGTTCGAAGGCTACG
GGCGTGGGGGTTCGGATCGG

Product: hydrogenase expression/formation protein HypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNFTCPTPLTGSDRILLGHGSGGTLSAALLSEIVLPAYNNTILERMEDQATLDINGITLAFTTDSFVVKPIFFPGGDIGS
LAVHGTINDLAMGGARPVALSVAMILEEGFAVSDLKRIAGSIGVAARACGVPIVTGDTKVVGRGSGDGIFLNTTGIGIVP
RGLQLSAANARPGDIVILSGSIGDHGIAILAQREGLSLDSAIESDSAPLHTLAASILNVTFEVRCMRDPTRGGLSSTLNE
IARQSGVGITIQETAVPVREDVRGVCELLGLDPLYVANEGKMVAIVPPQSAAAVLAAMRAHPLARDAAIIGEVTEGESGL
VLQRTAFGTHRIVTMLAGDQLPRIC

Sequences:

>Translated_345_residues
MNFTCPTPLTGSDRILLGHGSGGTLSAALLSEIVLPAYNNTILERMEDQATLDINGITLAFTTDSFVVKPIFFPGGDIGS
LAVHGTINDLAMGGARPVALSVAMILEEGFAVSDLKRIAGSIGVAARACGVPIVTGDTKVVGRGSGDGIFLNTTGIGIVP
RGLQLSAANARPGDIVILSGSIGDHGIAILAQREGLSLDSAIESDSAPLHTLAASILNVTFEVRCMRDPTRGGLSSTLNE
IARQSGVGITIQETAVPVREDVRGVCELLGLDPLYVANEGKMVAIVPPQSAAAVLAAMRAHPLARDAAIIGEVTEGESGL
VLQRTAFGTHRIVTMLAGDQLPRIC
>Mature_345_residues
MNFTCPTPLTGSDRILLGHGSGGTLSAALLSEIVLPAYNNTILERMEDQATLDINGITLAFTTDSFVVKPIFFPGGDIGS
LAVHGTINDLAMGGARPVALSVAMILEEGFAVSDLKRIAGSIGVAARACGVPIVTGDTKVVGRGSGDGIFLNTTGIGIVP
RGLQLSAANARPGDIVILSGSIGDHGIAILAQREGLSLDSAIESDSAPLHTLAASILNVTFEVRCMRDPTRGGLSSTLNE
IARQSGVGITIQETAVPVREDVRGVCELLGLDPLYVANEGKMVAIVPPQSAAAVLAAMRAHPLARDAAIIGEVTEGESGL
VLQRTAFGTHRIVTMLAGDQLPRIC

Specific function: May be involved in the maturation of the nifE hydrogenase [H]

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=338, Percent_Identity=45.8579881656805, Blast_Score=281, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 35546; Mature: 35546

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFTCPTPLTGSDRILLGHGSGGTLSAALLSEIVLPAYNNTILERMEDQATLDINGITLA
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCEEEE
FTTDSFVVKPIFFPGGDIGSLAVHGTINDLAMGGARPVALSVAMILEEGFAVSDLKRIAG
EECCCEEEEEEEECCCCCCCEEEEECHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
SIGVAARACGVPIVTGDTKVVGRGSGDGIFLNTTGIGIVPRGLQLSAANARPGDIVILSG
HHCHHHHHCCCEEEECCEEEEECCCCCEEEEEECCCEEECCCEEEEECCCCCCEEEEEEC
SIGDHGIAILAQREGLSLDSAIESDSAPLHTLAASILNVTFEVRCMRDPTRGGLSSTLNE
CCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHH
IARQSGVGITIQETAVPVREDVRGVCELLGLDPLYVANEGKMVAIVPPQSAAAVLAAMRA
HHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHC
HPLARDAAIIGEVTEGESGLVLQRTAFGTHRIVTMLAGDQLPRIC
CCCCCCCEEEEEECCCCCCEEEEEECCCCHHEEEEECCCCCCCCC
>Mature Secondary Structure
MNFTCPTPLTGSDRILLGHGSGGTLSAALLSEIVLPAYNNTILERMEDQATLDINGITLA
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCEEEE
FTTDSFVVKPIFFPGGDIGSLAVHGTINDLAMGGARPVALSVAMILEEGFAVSDLKRIAG
EECCCEEEEEEEECCCCCCCEEEEECHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
SIGVAARACGVPIVTGDTKVVGRGSGDGIFLNTTGIGIVPRGLQLSAANARPGDIVILSG
HHCHHHHHCCCEEEECCEEEEECCCCCEEEEEECCCEEECCCEEEEECCCCCCEEEEEEC
SIGDHGIAILAQREGLSLDSAIESDSAPLHTLAASILNVTFEVRCMRDPTRGGLSSTLNE
CCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHH
IARQSGVGITIQETAVPVREDVRGVCELLGLDPLYVANEGKMVAIVPPQSAAAVLAAMRA
HHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHC
HPLARDAAIIGEVTEGESGLVLQRTAFGTHRIVTMLAGDQLPRIC
CCCCCCCEEEEEECCCCCCEEEEEECCCCHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1526470 [H]