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The map label for this gene is tolB [H]

Identifier: 116625879

GI number: 116625879

Start: 8562673

End: 8563785

Strand: Reverse

Name: tolB [H]

Synonym: Acid_6833

Alternate gene names: 116625879

Gene position: 8563785-8562673 (Counterclockwise)

Preceding gene: 116625880

Following gene: 116625878

Centisome position: 85.93

GC content: 58.76

Gene sequence:

>1113_bases
ATGGATGAGCTGCATGTAGTGCCTGTGGGCGGCGGAGAATCAAACCGTTTGTCCGCCGGCGACCGAAGGCTGTCTGGCTT
GGCCTGGATGCCGGATGGCCGCGAAATTGTCTTCTCCTCCCGCCGCGGAATGCCACAGCAATTGTGGAACGTCAGCGCAT
CCGGCGGGACACCGCGGGCCTTACCCTTGGTTGGTGAGGGCACGGTCTGGCCCGCAATTTCCCGAACCGGACACCGCCTG
GCGTACGTTCGATCGACCTTCGATACCAACATTTGGCGTTTGGACTTGCCGCGGGAACCCAATTCTTCGTCCGGGCACCC
ATTGAAACTCATCGCTTCCACATGGCGCGATAACAATCCGCAGTATTCGCCGGATGGAACGAAGGTTGCGTTCGTCTCCG
ATCGCGCGGGCAGCCTCGATCTGTGGGTCACAAACGCCGATGGCTCAAGCCCGTTCCAATTGACCTCCCAGGGCGCGCAG
TCCACGCCAAGGTGGTCGCCGGACGGGCGCCGAATTGCGTTCGCAAGCTTTGCCAATAGCAACGAGAATATCTTCGTAAT
CGATGCCGAAGGGGGTGCAACCAAGCCGATCACGACCGGTCCTGCGAACCATAACTCTCCCTGCTGGTCATCCGATGGGC
GCTGGATCTATTTCACTTCCAACCGAACGGGGAAGGCGGAGATCTGGCGGGCGCCGGCAGAGGGCGGGTCGGAGAGCCAG
ATGACTCATCAAGGCGGCTGGGCTCCCCAGGAATCCCCGGACGGCAAAATGATCTACTACCAGAAGCAGTTCAGCGAGAC
GGCGATCTGGAAGATTCCGCCGGAGGGTGGCGTTGAATCGCCGGTGCTGGAATCGGCCACGGTAGCGAACCTTTACCTGT
GGCAGCCTTTCAACGACGACATATACTTTGTCCAGCGAGTGCGGAATAGCTCAATCTCCACCGAGGATCGCATTCAATTT
TTCGATTTCGCCACTCAAATGATCACCACACTCGGTCGCCTGGAAAAGCGCGCAAATCTCGGCCTCAGCATTTCGCCGGA
CCGGCGCAAGGTGATCTTCACCCAGATAGATCAGGACGAGCACGATATTATGCTGGTGGAGAATTTTCGCTAG

Upstream 100 bases:

>100_bases
CGACTGACCACCGCGCCTCCGGGGATCGGAGGCGATACCACACCGAAGTTCTCACTGGACGGTCAACGAATCGCCTTCGA
GCGGAATTTCCTATCTATAT

Downstream 100 bases:

>100_bases
CCGCCAAAAGAAACATACGTTACCTTTAGGCGGAAATGATCGTTCGGCTGCGCTCGGCGGATGCTTACATGTGAATCGAG
GACTAGTGCCGCAGTGCTGG

Product: transcriptional regulatory protein-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MDELHVVPVGGGESNRLSAGDRRLSGLAWMPDGREIVFSSRRGMPQQLWNVSASGGTPRALPLVGEGTVWPAISRTGHRL
AYVRSTFDTNIWRLDLPREPNSSSGHPLKLIASTWRDNNPQYSPDGTKVAFVSDRAGSLDLWVTNADGSSPFQLTSQGAQ
STPRWSPDGRRIAFASFANSNENIFVIDAEGGATKPITTGPANHNSPCWSSDGRWIYFTSNRTGKAEIWRAPAEGGSESQ
MTHQGGWAPQESPDGKMIYYQKQFSETAIWKIPPEGGVESPVLESATVANLYLWQPFNDDIYFVQRVRNSSISTEDRIQF
FDFATQMITTLGRLEKRANLGLSISPDRRKVIFTQIDQDEHDIMLVENFR

Sequences:

>Translated_370_residues
MDELHVVPVGGGESNRLSAGDRRLSGLAWMPDGREIVFSSRRGMPQQLWNVSASGGTPRALPLVGEGTVWPAISRTGHRL
AYVRSTFDTNIWRLDLPREPNSSSGHPLKLIASTWRDNNPQYSPDGTKVAFVSDRAGSLDLWVTNADGSSPFQLTSQGAQ
STPRWSPDGRRIAFASFANSNENIFVIDAEGGATKPITTGPANHNSPCWSSDGRWIYFTSNRTGKAEIWRAPAEGGSESQ
MTHQGGWAPQESPDGKMIYYQKQFSETAIWKIPPEGGVESPVLESATVANLYLWQPFNDDIYFVQRVRNSSISTEDRIQF
FDFATQMITTLGRLEKRANLGLSISPDRRKVIFTQIDQDEHDIMLVENFR
>Mature_370_residues
MDELHVVPVGGGESNRLSAGDRRLSGLAWMPDGREIVFSSRRGMPQQLWNVSASGGTPRALPLVGEGTVWPAISRTGHRL
AYVRSTFDTNIWRLDLPREPNSSSGHPLKLIASTWRDNNPQYSPDGTKVAFVSDRAGSLDLWVTNADGSSPFQLTSQGAQ
STPRWSPDGRRIAFASFANSNENIFVIDAEGGATKPITTGPANHNSPCWSSDGRWIYFTSNRTGKAEIWRAPAEGGSESQ
MTHQGGWAPQESPDGKMIYYQKQFSETAIWKIPPEGGVESPVLESATVANLYLWQPFNDDIYFVQRVRNSSISTEDRIQF
FDFATQMITTLGRLEKRANLGLSISPDRRKVIFTQIDQDEHDIMLVENFR

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=193, Percent_Identity=30.5699481865285, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 41123; Mature: 41123

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELHVVPVGGGESNRLSAGDRRLSGLAWMPDGREIVFSSRRGMPQQLWNVSASGGTPRA
CCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHHHEEECCCCCCCE
LPLVGEGTVWPAISRTGHRLAYVRSTFDTNIWRLDLPREPNSSSGHPLKLIASTWRDNNP
EEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCC
QYSPDGTKVAFVSDRAGSLDLWVTNADGSSPFQLTSQGAQSTPRWSPDGRRIAFASFANS
CCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCC
NENIFVIDAEGGATKPITTGPANHNSPCWSSDGRWIYFTSNRTGKAEIWRAPAEGGSESQ
CCEEEEEECCCCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCC
MTHQGGWAPQESPDGKMIYYQKQFSETAIWKIPPEGGVESPVLESATVANLYLWQPFNDD
EECCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCC
IYFVQRVRNSSISTEDRIQFFDFATQMITTLGRLEKRANLGLSISPDRRKVIFTQIDQDE
EEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCC
HDIMLVENFR
CCEEEEECCC
>Mature Secondary Structure
MDELHVVPVGGGESNRLSAGDRRLSGLAWMPDGREIVFSSRRGMPQQLWNVSASGGTPRA
CCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCHHHHHEEECCCCCCCE
LPLVGEGTVWPAISRTGHRLAYVRSTFDTNIWRLDLPREPNSSSGHPLKLIASTWRDNNP
EEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCC
QYSPDGTKVAFVSDRAGSLDLWVTNADGSSPFQLTSQGAQSTPRWSPDGRRIAFASFANS
CCCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCC
NENIFVIDAEGGATKPITTGPANHNSPCWSSDGRWIYFTSNRTGKAEIWRAPAEGGSESQ
CCEEEEEECCCCCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCC
MTHQGGWAPQESPDGKMIYYQKQFSETAIWKIPPEGGVESPVLESATVANLYLWQPFNDD
EECCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCC
IYFVQRVRNSSISTEDRIQFFDFATQMITTLGRLEKRANLGLSISPDRRKVIFTQIDQDE
EEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCC
HDIMLVENFR
CCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA