Click here to switch to the map view.

The map label for this gene is ansB [C]

Identifier: 116625795

GI number: 116625795

Start: 8450547

End: 8451041

Strand: Direct

Name: ansB [C]

Synonym: Acid_6745

Alternate gene names: 116625795

Gene position: 8450547-8451041 (Clockwise)

Preceding gene: 116625788

Following gene: 116625796

Centisome position: 84.8

GC content: 60.81

Gene sequence:

>495_bases
ATGGAACCGATCCGCATCCTGGTCACCGGTGGCACGTTCGATAAGGAATACAACGAACTCACCGGAGAATTGTTCTTCCA
GAAGACGCACGCCGGGGACATGCTCAAGCTGGGGCGCTGCCTGCTGGAGGTGGAGATCGAGACGCTGATGATGATCGACA
GCCTGCAGATGACGGATGCCGACCGACTGCATATATTGGATCGCTGCGCGGCGGTCCCGGAGAAGCGGCTGGTGATCACG
CATGGGACCGATACGATGGAGCAGACGGCGGCGGTGTTGGGTCCGGCGCTGCCGGAGAAGACGATCGTGCTGACGGGCGC
GATGGTGCCGTACACGTTCGGGAGTTCCGACGGGCTGTTTAATTTGGGGACGGCGATGGCGTTCGTGCAGACATTGGAGC
CGGGAGTTTACGTAGCGATGAACGGGCGCTGTTTCCCGTGGCACTCGGTGCGTAAGAACAAGAAAGAAGGCGTCTTCGAG
CTCCGGCGCGGCTAG

Upstream 100 bases:

>100_bases
CAGCGCCGAGGATGACGGCTATAGCCACCAGCAAGAGCCAGCGCATGCCTCGCATGGGTATAGCTCCAATATAGCGCGCT
ATGCTGAATGAAGCCCGCCT

Downstream 100 bases:

>100_bases
CGCAGGAAACCTTAGTCGATCACTGTCAAGTATTTTAAAACACATATTTTCAGTGGTTTAAGGCAAATTTCTGTCACTCT
CACGGCAAGTGTGCTAATAA

Product: asparaginase

Products: L-Aspartate; ammonia [C]

Alternate protein names: L-Asparaginase; Asparaginase/Glutaminase; Cytoplasmic L-Asparaginase I Protein; Glutamin-(Asparagin-)Ase Family Protein; Asparaginase Protein

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MEPIRILVTGGTFDKEYNELTGELFFQKTHAGDMLKLGRCLLEVEIETLMMIDSLQMTDADRLHILDRCAAVPEKRLVIT
HGTDTMEQTAAVLGPALPEKTIVLTGAMVPYTFGSSDGLFNLGTAMAFVQTLEPGVYVAMNGRCFPWHSVRKNKKEGVFE
LRRG

Sequences:

>Translated_164_residues
MEPIRILVTGGTFDKEYNELTGELFFQKTHAGDMLKLGRCLLEVEIETLMMIDSLQMTDADRLHILDRCAAVPEKRLVIT
HGTDTMEQTAAVLGPALPEKTIVLTGAMVPYTFGSSDGLFNLGTAMAFVQTLEPGVYVAMNGRCFPWHSVRKNKKEGVFE
LRRG
>Mature_164_residues
MEPIRILVTGGTFDKEYNELTGELFFQKTHAGDMLKLGRCLLEVEIETLMMIDSLQMTDADRLHILDRCAAVPEKRLVIT
HGTDTMEQTAAVLGPALPEKTIVLTGAMVPYTFGSSDGLFNLGTAMAFVQTLEPGVYVAMNGRCFPWHSVRKNKKEGVFE
LRRG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.1 [C]

Molecular weight: Translated: 18224; Mature: 18224

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIRILVTGGTFDKEYNELTGELFFQKTHAGDMLKLGRCLLEVEIETLMMIDSLQMTDA
CCCEEEEEECCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHECCCCCCCC
DRLHILDRCAAVPEKRLVITHGTDTMEQTAAVLGPALPEKTIVLTGAMVPYTFGSSDGLF
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCCCC
NLGTAMAFVQTLEPGVYVAMNGRCFPWHSVRKNKKEGVFELRRG
HHHHHHHHHHHCCCCEEEEECCEECCCHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MEPIRILVTGGTFDKEYNELTGELFFQKTHAGDMLKLGRCLLEVEIETLMMIDSLQMTDA
CCCEEEEEECCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHECCCCCCCC
DRLHILDRCAAVPEKRLVITHGTDTMEQTAAVLGPALPEKTIVLTGAMVPYTFGSSDGLF
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCCCC
NLGTAMAFVQTLEPGVYVAMNGRCFPWHSVRKNKKEGVFELRRG
HHHHHHHHHHHCCCCEEEEECCEECCCHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Asparagine; H2O [C]

Specific reaction: L-Asparagine + H2O --> L-Aspartate + ammonia [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA