Click here to switch to the map view.

The map label for this gene is pcpB [H]

Identifier: 116625786

GI number: 116625786

Start: 8438280

End: 8439824

Strand: Reverse

Name: pcpB [H]

Synonym: Acid_6736

Alternate gene names: 116625786

Gene position: 8439824-8438280 (Counterclockwise)

Preceding gene: 116625789

Following gene: 116625785

Centisome position: 84.69

GC content: 62.07

Gene sequence:

>1545_bases
ATGGCAACGACCTCCGTGTTGATCGCCGGGGCTGGGCCGACCGGCCTGAACCTCGCGCTGAGTCTTGCGCGCCGCGGTGT
GGCCTTTCGACTCATTTCCGATGCCGCGGGCCCCGGCGAGCATTCACGTGCCATGGTCGTGCAGGCACGCACCCTGGAGT
TTTACGATCAGTTGGGCTTTGCCGATGAGGTCGTGGCACAGGGCGTCAAGGCAGGCGCTGCCCACCTGCGTCAAGGCGAC
CAGAACAGTCGGGAGGTGGTTACTGTCACCTTCGCGGATTTGGGGGCGGGGCTCACGCCGTATCCTTTCGCGCTCGCGTA
CCCGCAGGACGATCATGAGCGCTTCCTGATCGACAAGCTCAAAGCCGCCGGTCGGACCGTTGAATGGAATTCCCGGCTCA
TAACGTTTACCCAGGACGAAGACGGCGTGCGTGCCAAGGTGGTGCGCGACGGCCACACGGAAGAAACCCGTGCCACGTAC
ATCTGCGGTTGCGATGGCGCCCATAGCTGCGTGCGCGAGACTCTGTCGGTAGATTTCCCGGGCGGCACCTACGACCAGCT
CTTTTACGTCGCCGACGTAAAAATCACGCGCGGTTTTGACCGCGATTTGTACATCAATCTTGGCAAGCACGTCCTGACTC
TCATGTTTCCGGTGCGTTCAAGCGGAATGCAGCGTCTAATCGGTCTGGTGCCGGCGGACCTGTCGCAAGACCAGAAACTG
ACTTTTGAGGACCTCCGGCGCCAGGTCGAGGCGCAATTGGACATTCGCGTCACGGACGTCAACTGGTTCGCCACGTACCG
CGTCCACCATCGCGTAGCCGAGCGGTTTCGGATTGGCCGCGCGTTTCTTTTGGGGGACGCGGGACACATTCACAGCCCCG
CCGGCGGGCAGGGCATGAACACCGGCATCGGTGATGCGATCAATCTTGCCTGGAAACTCGCGCACGTGCTTGATCGGCGT
GCCGACCCGTCGCTGCTGGATACGTACGAGACCGAGCGGATCGGATTCGCGCGCTCGCTCGTGTCCACTACCGATCGCGC
CTTTACGCCGATAGTCGCTGACGGTTTTCGGGGCGAGATTACCCGCCGGCTCCTGGCCCCGCTGTTTATGACGGCAGCCA
CGCGCTTCACTCTAAGCCGGCACGCGTTGTTTCGCGTCATCTCGCAAATGCGGATTCACTATGCCGGCAGCCCGCTGAGT
GAAGGGCAGGCAGGCGAGGTGCATGGCGGCGACCGTCTGCCCTGGATCGGGGCAGGTGCGCAGGACAACTTCGCCCCACT
ACGCTCGCTTGATTGGCAATTGCATGTGTACGGAACGCTCACTCCCGAACTCGCAATGGCATGTGCGGAGCTTGGCCTTC
CGGTGCAGACCTTTGCGTGGACCGAGGCAGCAGACCACGGCGGAATAAAACGTGACGCCGCCTATCTCGTGCGGCCTGAT
GGCCATGTTGCGCTCGCAGCACCAGAGCAGAGTGTTACGGCGCTGAAGTCTTTCGTGGACCGCTGGCATATGCGATTCAC
ACAAGCTTCTCCCGCGCATGACTGA

Upstream 100 bases:

>100_bases
CCGGAACGACAATATCCTAAAGGTAGGTAGATGAAGGGTAACGATGAACGCCTGGCATGCTCAAACTCATGCGGCCACAT
CGTGATCCCGGGAGTAAGAA

Downstream 100 bases:

>100_bases
ATTCGCAGCGCACCGCGCCGGAAGGGGTTGGCCCCTGTGATAAACTGCCAGCGAATGTTTGGGGGAGTACATGAAGGCGC
CAATCCTTTATCGAATCGCA

Product: monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MATTSVLIAGAGPTGLNLALSLARRGVAFRLISDAAGPGEHSRAMVVQARTLEFYDQLGFADEVVAQGVKAGAAHLRQGD
QNSREVVTVTFADLGAGLTPYPFALAYPQDDHERFLIDKLKAAGRTVEWNSRLITFTQDEDGVRAKVVRDGHTEETRATY
ICGCDGAHSCVRETLSVDFPGGTYDQLFYVADVKITRGFDRDLYINLGKHVLTLMFPVRSSGMQRLIGLVPADLSQDQKL
TFEDLRRQVEAQLDIRVTDVNWFATYRVHHRVAERFRIGRAFLLGDAGHIHSPAGGQGMNTGIGDAINLAWKLAHVLDRR
ADPSLLDTYETERIGFARSLVSTTDRAFTPIVADGFRGEITRRLLAPLFMTAATRFTLSRHALFRVISQMRIHYAGSPLS
EGQAGEVHGGDRLPWIGAGAQDNFAPLRSLDWQLHVYGTLTPELAMACAELGLPVQTFAWTEAADHGGIKRDAAYLVRPD
GHVALAAPEQSVTALKSFVDRWHMRFTQASPAHD

Sequences:

>Translated_514_residues
MATTSVLIAGAGPTGLNLALSLARRGVAFRLISDAAGPGEHSRAMVVQARTLEFYDQLGFADEVVAQGVKAGAAHLRQGD
QNSREVVTVTFADLGAGLTPYPFALAYPQDDHERFLIDKLKAAGRTVEWNSRLITFTQDEDGVRAKVVRDGHTEETRATY
ICGCDGAHSCVRETLSVDFPGGTYDQLFYVADVKITRGFDRDLYINLGKHVLTLMFPVRSSGMQRLIGLVPADLSQDQKL
TFEDLRRQVEAQLDIRVTDVNWFATYRVHHRVAERFRIGRAFLLGDAGHIHSPAGGQGMNTGIGDAINLAWKLAHVLDRR
ADPSLLDTYETERIGFARSLVSTTDRAFTPIVADGFRGEITRRLLAPLFMTAATRFTLSRHALFRVISQMRIHYAGSPLS
EGQAGEVHGGDRLPWIGAGAQDNFAPLRSLDWQLHVYGTLTPELAMACAELGLPVQTFAWTEAADHGGIKRDAAYLVRPD
GHVALAAPEQSVTALKSFVDRWHMRFTQASPAHD
>Mature_513_residues
ATTSVLIAGAGPTGLNLALSLARRGVAFRLISDAAGPGEHSRAMVVQARTLEFYDQLGFADEVVAQGVKAGAAHLRQGDQ
NSREVVTVTFADLGAGLTPYPFALAYPQDDHERFLIDKLKAAGRTVEWNSRLITFTQDEDGVRAKVVRDGHTEETRATYI
CGCDGAHSCVRETLSVDFPGGTYDQLFYVADVKITRGFDRDLYINLGKHVLTLMFPVRSSGMQRLIGLVPADLSQDQKLT
FEDLRRQVEAQLDIRVTDVNWFATYRVHHRVAERFRIGRAFLLGDAGHIHSPAGGQGMNTGIGDAINLAWKLAHVLDRRA
DPSLLDTYETERIGFARSLVSTTDRAFTPIVADGFRGEITRRLLAPLFMTAATRFTLSRHALFRVISQMRIHYAGSPLSE
GQAGEVHGGDRLPWIGAGAQDNFAPLRSLDWQLHVYGTLTPELAMACAELGLPVQTFAWTEAADHGGIKRDAAYLVRPDG
HVALAAPEQSVTALKSFVDRWHMRFTQASPAHD

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=419, Percent_Identity=27.4463007159904, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789274, Length=386, Percent_Identity=23.5751295336788, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 56447; Mature: 56315

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTSVLIAGAGPTGLNLALSLARRGVAFRLISDAAGPGEHSRAMVVQARTLEFYDQLGF
CCCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHCCC
ADEVVAQGVKAGAAHLRQGDQNSREVVTVTFADLGAGLTPYPFALAYPQDDHERFLIDKL
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCCEEEECCCCCCHHHHHHHH
KAAGRTVEWNSRLITFTQDEDGVRAKVVRDGHTEETRATYICGCDGAHSCVRETLSVDFP
HHCCCEEEECCEEEEEECCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHEECCC
GGTYDQLFYVADVKITRGFDRDLYINLGKHVLTLMFPVRSSGMQRLIGLVPADLSQDQKL
CCCHHHEEEEEEEEEECCCCCCEEEECCHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TFEDLRRQVEAQLDIRVTDVNWFATYRVHHRVAERFRIGRAFLLGDAGHIHSPAGGQGMN
CHHHHHHHHCCEEEEEEEEEEEEEHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC
TGIGDAINLAWKLAHVLDRRADPSLLDTYETERIGFARSLVSTTDRAFTPIVADGFRGEI
CCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHH
TRRLLAPLFMTAATRFTLSRHALFRVISQMRIHYAGSPLSEGQAGEVHGGDRLPWIGAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCC
QDNFAPLRSLDWQLHVYGTLTPELAMACAELGLPVQTFAWTEAADHGGIKRDAAYLVRPD
CCCCCHHHCCCEEEEEEEECCHHHHHHHHHHCCCHHHEEECCHHCCCCCCCCCEEEECCC
GHVALAAPEQSVTALKSFVDRWHMRFTQASPAHD
CCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ATTSVLIAGAGPTGLNLALSLARRGVAFRLISDAAGPGEHSRAMVVQARTLEFYDQLGF
CCCEEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHCCC
ADEVVAQGVKAGAAHLRQGDQNSREVVTVTFADLGAGLTPYPFALAYPQDDHERFLIDKL
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCCEEEECCCCCCHHHHHHHH
KAAGRTVEWNSRLITFTQDEDGVRAKVVRDGHTEETRATYICGCDGAHSCVRETLSVDFP
HHCCCEEEECCEEEEEECCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHEECCC
GGTYDQLFYVADVKITRGFDRDLYINLGKHVLTLMFPVRSSGMQRLIGLVPADLSQDQKL
CCCHHHEEEEEEEEEECCCCCCEEEECCHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TFEDLRRQVEAQLDIRVTDVNWFATYRVHHRVAERFRIGRAFLLGDAGHIHSPAGGQGMN
CHHHHHHHHCCEEEEEEEEEEEEEHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCC
TGIGDAINLAWKLAHVLDRRADPSLLDTYETERIGFARSLVSTTDRAFTPIVADGFRGEI
CCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHH
TRRLLAPLFMTAATRFTLSRHALFRVISQMRIHYAGSPLSEGQAGEVHGGDRLPWIGAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCC
QDNFAPLRSLDWQLHVYGTLTPELAMACAELGLPVQTFAWTEAADHGGIKRDAAYLVRPD
CCCCCHHHCCCEEEEEEEECCHHHHHHHHHHCCCHHHEEECCHHCCCCCCCCCEEEECCC
GHVALAAPEQSVTALKSFVDRWHMRFTQASPAHD
CCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]