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The map label for this gene is entA [C]

Identifier: 116625783

GI number: 116625783

Start: 8435870

End: 8436847

Strand: Direct

Name: entA [C]

Synonym: Acid_6733

Alternate gene names: 116625783

Gene position: 8435870-8436847 (Clockwise)

Preceding gene: 116625782

Following gene: 116625784

Centisome position: 84.65

GC content: 63.7

Gene sequence:

>978_bases
ATGACGATTACATTGGGAGCGACATCGACCGCAGACGACGTTTTGTCGGGCGTCAACCTGCATGGAAAACGGATTCTGGT
TACCGGCGTGTCCGCGGGAATCGGAGTAGAGACGGCGCGTGCTCTCGCCGCGCACGGTGCGCACGTGGTGGGGGCGGCGA
GAGATCTGGCGAAGGCCGAGGCGGCAACGGCGCAGGTGCAAAAGGATGCCGCGGCGAACGGCGGCGGCTTCGAGTTGACC
GCGCTCGACCTGGCCGATCTGAAGAGCGTCCGCGCCTGCGCCGACGGCCTGATCGCCAAGGGAGAGCCGTTTGACGTGGT
GATCGCCAATGCCGGCGTGATGGCGACGCCATTTGGCCACACCGCGGACGGTTTTGAGACGCAGTTCGGCACCAATCATC
TGGGCCACTTCGTCCTGATCAACCGCATTGCCTCTTTGATTCGCACGGGCGGGCGGCTGGTGAACCTGTCGTCCGCCGGC
CACCGCATTTCGAACGTAGACGTTGAGGATCCCAACTTCGAGCGCACGCCCTACGACCCGATGATCGCTTATGGACGGTC
GAAGACTGCGAACATCCTTTTCGCTGTTGCCTTCGACCAGCGGCATCGGAGTCGTGGGGTACGCGCCACCGCGGTTCATC
CGGGGGCCATACAGACCGAACTGGGCCGGTACATGGAGCGCGCCCAGCTGGAAAGCATGATTGACCGGATTAACCAGCAA
TTGGCCGCAGAAGGAAAGGGGCCCTTCGAATGGAAGACGATTCCGCAGGGAGCGGCCACCTCGGTGTGGGCCGGAGTACT
CGCTCCGGCTGACGAGATCGGCGGCCGGTATTGCGCGAACTGCCAGGTCGGCCGCATCGTGCGGGGCGACGCCGTCATAA
CGGCGGCCGGAGAGGGAGTGCGCGAATATGCACTCGATCCGCACAACGCCGCGGCACTGTGGAAGAAGAGCGAGGAACTG
GTCGGAGAATCGTACTGA

Upstream 100 bases:

>100_bases
GCAGACGCGGCAACGGCTCCAATGGCAGCCAACCGGCCCCGGGCTGATTGCCGATCTCGAACAAATGCACTGGCTGAAAG
CTTAATCAAGGAGGAGAGAA

Downstream 100 bases:

>100_bases
GTCTTGCAACTATATAAGGGGGAATAAGCGGGATGAGAGAACGAAATAACGTGGTGCGCAGCTTTACGTTTCCCGGCACT
TCGCTGACTGTGAACCGGAT

Product: short-chain dehydrogenase/reductase SDR

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTITLGATSTADDVLSGVNLHGKRILVTGVSAGIGVETARALAAHGAHVVGAARDLAKAEAATAQVQKDAAANGGGFELT
ALDLADLKSVRACADGLIAKGEPFDVVIANAGVMATPFGHTADGFETQFGTNHLGHFVLINRIASLIRTGGRLVNLSSAG
HRISNVDVEDPNFERTPYDPMIAYGRSKTANILFAVAFDQRHRSRGVRATAVHPGAIQTELGRYMERAQLESMIDRINQQ
LAAEGKGPFEWKTIPQGAATSVWAGVLAPADEIGGRYCANCQVGRIVRGDAVITAAGEGVREYALDPHNAAALWKKSEEL
VGESY

Sequences:

>Translated_325_residues
MTITLGATSTADDVLSGVNLHGKRILVTGVSAGIGVETARALAAHGAHVVGAARDLAKAEAATAQVQKDAAANGGGFELT
ALDLADLKSVRACADGLIAKGEPFDVVIANAGVMATPFGHTADGFETQFGTNHLGHFVLINRIASLIRTGGRLVNLSSAG
HRISNVDVEDPNFERTPYDPMIAYGRSKTANILFAVAFDQRHRSRGVRATAVHPGAIQTELGRYMERAQLESMIDRINQQ
LAAEGKGPFEWKTIPQGAATSVWAGVLAPADEIGGRYCANCQVGRIVRGDAVITAAGEGVREYALDPHNAAALWKKSEEL
VGESY
>Mature_324_residues
TITLGATSTADDVLSGVNLHGKRILVTGVSAGIGVETARALAAHGAHVVGAARDLAKAEAATAQVQKDAAANGGGFELTA
LDLADLKSVRACADGLIAKGEPFDVVIANAGVMATPFGHTADGFETQFGTNHLGHFVLINRIASLIRTGGRLVNLSSAGH
RISNVDVEDPNFERTPYDPMIAYGRSKTANILFAVAFDQRHRSRGVRATAVHPGAIQTELGRYMERAQLESMIDRINQQL
AAEGKGPFEWKTIPQGAATSVWAGVLAPADEIGGRYCANCQVGRIVRGDAVITAAGEGVREYALDPHNAAALWKKSEELV
GESY

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI186928839, Length=309, Percent_Identity=35.2750809061489, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI7706523, Length=326, Percent_Identity=34.0490797546012, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI10190746, Length=317, Percent_Identity=33.1230283911672, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI166795268, Length=273, Percent_Identity=36.6300366300366, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI225579078, Length=211, Percent_Identity=40.2843601895735, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI193804850, Length=263, Percent_Identity=32.6996197718631, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI19923931, Length=145, Percent_Identity=44.1379310344828, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI146231950, Length=316, Percent_Identity=30.6962025316456, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI7705421, Length=235, Percent_Identity=28.5106382978723, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI72534732, Length=191, Percent_Identity=26.1780104712042, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193207620, Length=326, Percent_Identity=30.9815950920245, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17532791, Length=323, Percent_Identity=30.9597523219814, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17532805, Length=326, Percent_Identity=30.9815950920245, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI71992402, Length=322, Percent_Identity=30.7453416149068, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17535059, Length=319, Percent_Identity=30.0940438871473, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32565532, Length=221, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17558006, Length=273, Percent_Identity=29.6703296703297, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17505416, Length=265, Percent_Identity=29.0566037735849, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001364, Length=220, Percent_Identity=29.0909090909091, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324820, Length=235, Percent_Identity=31.063829787234, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI20129347, Length=330, Percent_Identity=33.030303030303, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI22024069, Length=306, Percent_Identity=34.3137254901961, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI28573296, Length=312, Percent_Identity=34.6153846153846, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45554248, Length=208, Percent_Identity=40.8653846153846, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640117, Length=208, Percent_Identity=40.8653846153846, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19921754, Length=303, Percent_Identity=32.3432343234323, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24586328, Length=327, Percent_Identity=31.1926605504587, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24647946, Length=325, Percent_Identity=30.1538461538462, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161076371, Length=265, Percent_Identity=32.8301886792453, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45553409, Length=306, Percent_Identity=30.718954248366, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647948, Length=306, Percent_Identity=30.718954248366, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24656154, Length=294, Percent_Identity=32.312925170068, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922628, Length=294, Percent_Identity=32.312925170068, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24647931, Length=310, Percent_Identity=24.5161290322581, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 34150; Mature: 34019

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITLGATSTADDVLSGVNLHGKRILVTGVSAGIGVETARALAAHGAHVVGAARDLAKAE
CEEEECCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
AATAQVQKDAAANGGGFELTALDLADLKSVRACADGLIAKGEPFDVVIANAGVMATPFGH
HHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEECCCCC
TADGFETQFGTNHLGHFVLINRIASLIRTGGRLVNLSSAGHRISNVDVEDPNFERTPYDP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCCCCCCH
MIAYGRSKTANILFAVAFDQRHRSRGVRATAVHPGAIQTELGRYMERAQLESMIDRINQQ
HHHCCCCCCCEEEEEEEECHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAAEGKGPFEWKTIPQGAATSVWAGVLAPADEIGGRYCANCQVGRIVRGDAVITAAGEGV
HHHCCCCCCCEEECCCCCHHHHHHHHHCCHHHHCCEEECCCCCCEEEECCEEEEECCCCH
REYALDPHNAAALWKKSEELVGESY
HHHCCCCCCHHHHHHCCHHHCCCCC
>Mature Secondary Structure 
TITLGATSTADDVLSGVNLHGKRILVTGVSAGIGVETARALAAHGAHVVGAARDLAKAE
EEEECCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
AATAQVQKDAAANGGGFELTALDLADLKSVRACADGLIAKGEPFDVVIANAGVMATPFGH
HHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEECCCCC
TADGFETQFGTNHLGHFVLINRIASLIRTGGRLVNLSSAGHRISNVDVEDPNFERTPYDP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCCCCCCH
MIAYGRSKTANILFAVAFDQRHRSRGVRATAVHPGAIQTELGRYMERAQLESMIDRINQQ
HHHCCCCCCCEEEEEEEECHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAAEGKGPFEWKTIPQGAATSVWAGVLAPADEIGGRYCANCQVGRIVRGDAVITAAGEGV
HHHCCCCCCCEEECCCCCHHHHHHHHHCCHHHHCCEEECCCCCCEEEECCEEEEECCCCH
REYALDPHNAAALWKKSEELVGESY
HHHCCCCCCHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]