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The map label for this gene is dus [H]

Identifier: 116625730

GI number: 116625730

Start: 8381773

End: 8382786

Strand: Direct

Name: dus [H]

Synonym: Acid_6679

Alternate gene names: 116625730

Gene position: 8381773-8382786 (Clockwise)

Preceding gene: 116625726

Following gene: 116625731

Centisome position: 84.11

GC content: 62.52

Gene sequence:

>1014_bases
ATGGAAGTATTTCCTCAACAATTCTCCATTGGCCCGCTGACCATCGCGCCGGCCACAGTGCTCGCCCCCATGGCGGGAGT
GACGGACACGGTCTTCCGCCGCTTCATCCGCAATCTGGACGGCTGCGGCCTTATTATGACCGAGTTCACCAGCTCCCATG
GAGTCAGCGCCGCGCTCAAGGCCAAAAAGCCCAACAAGACCATGAAGCGCTACCTGAACTTCGAACCCGATGAGCATCCC
ATCTCCGCCCAGCTCTTCGGCGCCGACCCCGCCGTCATGGCCGACGCCGCCCGCGTCTGCCAGGATCTCGGCTTCGACAT
TCTCGATCTCAACTTCGGCTGCCCCGTCAACAAAGTCGTCAAGTGCAACGGCGGCTCCGGCCTGCTGCGCGACCTGCCGC
TCGTCGAGAAGCTGCTGCGCTCTGTGCGCGCCGCCATCACCATCCCCTTCACCATGAAGTTCCGCGCCGGCTGGAATGAC
CGGGAGTTGGTCGCCGTCGCCATGGCAAAGCTCGCCGAGGATTGCGGCCTCCAGGCCATCGCCCTCCATCCCCGCACCCG
CGAGCAGGGCTACGCCGGCAAAGCCGATTGGACCCGCATCGCCGAAGTCAAGGCAGCCGTCAAAATCCCCGTGATCGGCA
ATGGCGATATCGTCACGCCCGAGGACGCCGTCCGCATGGTCCGCGAAACCAACTGCGACGCCGTCATGATCGGCCGCGGC
GCCAGTTCCAATCCCTGGATCTTCCGCCAGATCCAGCAGTACCTCGCCACCGGCCGCTACGACGAACCCACCCACGGCGA
TCGCTACCAGATGATGCGTCAATATTACGACATGCTGATCGCCCGCCGCGAAGACGATGCCGTTGGAAAAATGAAACAAT
TCGCCACCTATTTTACCCATGGAGTGCGCCACGGTGCCCACCTGCGCGCCTCCATCTATCAGGCCCACGAAACCGCCGCC
ATTCTTGAACTTGTCGACCAGTTCTTTCACTCCGAACCGGTGCTCGCTCAGTGA

Upstream 100 bases:

>100_bases
CAGCGGTTTCATCTCATTTTCCTTGTACATCCCAACTGACGAACCCCGCCTCGTTGGGGTTTCGTGCGCGGTTGTGTTCT
AAAATCGAATTAGATCCTGA

Downstream 100 bases:

>100_bases
AAAAAGTTCAATTGATCACCGATGGCGCCTGCCTCGGCAACCCCGGTCCCGGCGGCTGGTCCGCCATCCTTCGTTTTGAG
GAACAGAAGAAGGAGTTGTG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MEVFPQQFSIGPLTIAPATVLAPMAGVTDTVFRRFIRNLDGCGLIMTEFTSSHGVSAALKAKKPNKTMKRYLNFEPDEHP
ISAQLFGADPAVMADAARVCQDLGFDILDLNFGCPVNKVVKCNGGSGLLRDLPLVEKLLRSVRAAITIPFTMKFRAGWND
RELVAVAMAKLAEDCGLQAIALHPRTREQGYAGKADWTRIAEVKAAVKIPVIGNGDIVTPEDAVRMVRETNCDAVMIGRG
ASSNPWIFRQIQQYLATGRYDEPTHGDRYQMMRQYYDMLIARREDDAVGKMKQFATYFTHGVRHGAHLRASIYQAHETAA
ILELVDQFFHSEPVLAQ

Sequences:

>Translated_337_residues
MEVFPQQFSIGPLTIAPATVLAPMAGVTDTVFRRFIRNLDGCGLIMTEFTSSHGVSAALKAKKPNKTMKRYLNFEPDEHP
ISAQLFGADPAVMADAARVCQDLGFDILDLNFGCPVNKVVKCNGGSGLLRDLPLVEKLLRSVRAAITIPFTMKFRAGWND
RELVAVAMAKLAEDCGLQAIALHPRTREQGYAGKADWTRIAEVKAAVKIPVIGNGDIVTPEDAVRMVRETNCDAVMIGRG
ASSNPWIFRQIQQYLATGRYDEPTHGDRYQMMRQYYDMLIARREDDAVGKMKQFATYFTHGVRHGAHLRASIYQAHETAA
ILELVDQFFHSEPVLAQ
>Mature_337_residues
MEVFPQQFSIGPLTIAPATVLAPMAGVTDTVFRRFIRNLDGCGLIMTEFTSSHGVSAALKAKKPNKTMKRYLNFEPDEHP
ISAQLFGADPAVMADAARVCQDLGFDILDLNFGCPVNKVVKCNGGSGLLRDLPLVEKLLRSVRAAITIPFTMKFRAGWND
RELVAVAMAKLAEDCGLQAIALHPRTREQGYAGKADWTRIAEVKAAVKIPVIGNGDIVTPEDAVRMVRETNCDAVMIGRG
ASSNPWIFRQIQQYLATGRYDEPTHGDRYQMMRQYYDMLIARREDDAVGKMKQFATYFTHGVRHGAHLRASIYQAHETAA
ILELVDQFFHSEPVLAQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=291, Percent_Identity=29.553264604811, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI239788462, Length=291, Percent_Identity=29.553264604811, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI40807366, Length=232, Percent_Identity=30.1724137931034, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI31742496, Length=230, Percent_Identity=28.2608695652174, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789660, Length=255, Percent_Identity=40.7843137254902, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1788462, Length=291, Percent_Identity=30.2405498281787, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI145693211, Length=296, Percent_Identity=27.7027027027027, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=287, Percent_Identity=25.7839721254355, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=30.8641975308642, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=172, Percent_Identity=31.3953488372093, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17510279, Length=211, Percent_Identity=30.8056872037915, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=28.3898305084746, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=197, Percent_Identity=30.9644670050761, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=277, Percent_Identity=32.4909747292419, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24580595, Length=238, Percent_Identity=32.3529411764706, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920448, Length=238, Percent_Identity=32.3529411764706, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921524, Length=233, Percent_Identity=27.8969957081545, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45549423, Length=175, Percent_Identity=32.5714285714286, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37388; Mature: 37388

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFPQQFSIGPLTIAPATVLAPMAGVTDTVFRRFIRNLDGCGLIMTEFTSSHGVSAALK
CCCCCCCCCCCCEEECHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEHHCCCCCCEEEEE
AKKPNKTMKRYLNFEPDEHPISAQLFGADPAVMADAARVCQDLGFDILDLNFGCPVNKVV
CCCCCHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEE
KCNGGSGLLRDLPLVEKLLRSVRAAITIPFTMKFRAGWNDRELVAVAMAKLAEDCGLQAI
EECCCCCHHHHHHHHHHHHHHHHHHEECCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEE
ALHPRTREQGYAGKADWTRIAEVKAAVKIPVIGNGDIVTPEDAVRMVRETNCDAVMIGRG
EECCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCEECHHHHHHHHHHCCCCEEEEECC
ASSNPWIFRQIQQYLATGRYDEPTHGDRYQMMRQYYDMLIARREDDAVGKMKQFATYFTH
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GVRHGAHLRASIYQAHETAAILELVDQFFHSEPVLAQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEVFPQQFSIGPLTIAPATVLAPMAGVTDTVFRRFIRNLDGCGLIMTEFTSSHGVSAALK
CCCCCCCCCCCCEEECHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEHHCCCCCCEEEEE
AKKPNKTMKRYLNFEPDEHPISAQLFGADPAVMADAARVCQDLGFDILDLNFGCPVNKVV
CCCCCHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEE
KCNGGSGLLRDLPLVEKLLRSVRAAITIPFTMKFRAGWNDRELVAVAMAKLAEDCGLQAI
EECCCCCHHHHHHHHHHHHHHHHHHEECCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEE
ALHPRTREQGYAGKADWTRIAEVKAAVKIPVIGNGDIVTPEDAVRMVRETNCDAVMIGRG
EECCCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCEECHHHHHHHHHHCCCCEEEEECC
ASSNPWIFRQIQQYLATGRYDEPTHGDRYQMMRQYYDMLIARREDDAVGKMKQFATYFTH
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GVRHGAHLRASIYQAHETAAILELVDQFFHSEPVLAQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]