Click here to switch to the map view.

The map label for this gene is lacZ [H]

Identifier: 116625719

GI number: 116625719

Start: 8371120

End: 8372904

Strand: Reverse

Name: lacZ [H]

Synonym: Acid_6668

Alternate gene names: 116625719

Gene position: 8372904-8371120 (Counterclockwise)

Preceding gene: 116625720

Following gene: 116625718

Centisome position: 84.02

GC content: 63.08

Gene sequence:

>1785_bases
ATGCGTATTCAAACGGCGGCGCTGATTTTCTGGGCGTCCGCCTCTTCCTTATTTGCCGCGGATTGGAAGGTTGCCGACAA
TCCTCTCTCGACGCCCTGGACTTCGAAAGTCACGGCGGAACCCGCGCTGCCGGAGTATCCACGGCCGCAGCTCGAGCGCG
CGAAATGGACGAACCTGAACGGGCTGTGGGATTACGCGATCCGTGCGAAGGGCGAAGGGCGGCCGGGGCAGTCCGAGGGG
AAATTGCTGGTGCCGTTCCCGGTGGAATCGGCGCTCTCGGGGGTGAAGCGGCCGCTGACCCCGGACCAACGGCTTTGGTA
CAGCCGCAGCTTCAACGCCTCGGCGGCCAAGGGGTCGCGGCTACTGCTGCACTTTGGCGCGGTGGATTGGCGCGCGGAGG
TGTTCGTCAACGGCAAGGCGGTGGGCACGCACGAGGGCGGGTACGATCCGTTCACGTTCGACATCACCGACGCATTGAAA
GCGGGCGGGGCGCAGGAAGTTACGGTGGCGGTGTGGGATCCGACGGATACGGCACTGCAGCCGCGGGGCAAGCAGGTCCT
CAGCCCCAACGGCATCTGGTACACGGCGGTCTCCGGCATCTGGCAGACGGTGTGGATCGAGCCGGTCCCGGCGGTGTACA
TCGAATCGATCGCGATGACGCCGGACATTGACGCGCATCAACTGCGCCTGACGGTGCGCTCGGCTGCGAGCGGCGATTTC
ACGGCCACGGCCAGGCTGAGAGGGCGCAGCGTGGGGAGCATTTCCGGGAGGACGAATGCGGAAGTGCGGCTGCCGCTGAA
CGTACTGGAGTTGTGGTCGCCGGATTCGCCGACGCTGTACGATCTGGAAATCGCGTTGAAATCGGGCGACGCGGTGAAGA
GCTACTTCGGCATGCGCAGCGTGGAAATGCGCGCGGATGCGGAGGGGCACAATCGGATTTTTCTAAATCATCAACCGCTT
TTCCAGATCGGTCCGCTGGATCAGGGCTGGTGGCCCGACGGACTCTACACCGCGCCTACCGATGAGGCCCTGCGGTTCGA
CATCGAAGCCTTGAAGAAGATGGGTTTCAACATGGCGCGCAAACATGTGAAAGTAGAGCCGGCGCGCTGGTATTACTGGT
GCGACAAGCTCGGTTTCCTGGTGTGGCAGGACATGCCGAGCGCGATGACGAAGACTCCGCCGAGCAATGTGCGCAAGGGC
GCGGCCGAAGACGCGCAGTTTTCCGCCGGGGAGGCCGCAGCGTGGGAGCGCGAGTTGAAGGCGATCATCGCCGCCAATGG
GAATTCGCCGAGCATTATCGTGTGGGTGCCGTTCAACGAAGGATGGGGACAGCACGCCACGAACGATGTGCTGAAGATGG
TCAAGACGCTGGATCCGAGCCGGCTGGTGGATGGACCCAGCGGCTGGGAGGATCGCGGGTGGGGCGACCTCAAGGACATG
CACAACTATCCCGGGCCCGGCATGTTTCCGGCCTTGAACGACCGCGCGAGCGTGCTCGGCGAATTCGGCGGACTTGGTCT
GCCGCTGGCGGGGCATCTTTGGTGGGACAAACGCAATTGGGGATACAAGACATTCGAAGATCGCGCGCAATTGCAGGCGG
CGTATGAAGGGCTGATGGATGGGCTGGGGCCGCTGGTGAAGAATGGGCTGGCCGCAGCGATTTATACGCAGACCACGGAT
GTGGAGGGCGAGATCAACGGGCTGATGACGTATGACCGGCAGGTGATGAAGTTCGATGTGGGAAAGCTGGCCGAGATGCA
CCGGCGGCTGATTTTGGGGAAGTGA

Upstream 100 bases:

>100_bases
GGATCATGTGCGGGCGCTGCTGGCGAGTTGGAGCGACGTGCGCGTAATCCCCGACCTGGCCGGCATCCCGCGGGTAATTG
CCGCCCGATAAGATGGGCAT

Downstream 100 bases:

>100_bases
CGTGGAACCGATTCGTCTGCGGGTATTTCGGATTGTGGCGGAGGAGCTCAGCTTCACGCGGGCGGCGGAACGGCTGTTTC
TGACGCAGCCGGCGGTCACG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MRIQTAALIFWASASSLFAADWKVADNPLSTPWTSKVTAEPALPEYPRPQLERAKWTNLNGLWDYAIRAKGEGRPGQSEG
KLLVPFPVESALSGVKRPLTPDQRLWYSRSFNASAAKGSRLLLHFGAVDWRAEVFVNGKAVGTHEGGYDPFTFDITDALK
AGGAQEVTVAVWDPTDTALQPRGKQVLSPNGIWYTAVSGIWQTVWIEPVPAVYIESIAMTPDIDAHQLRLTVRSAASGDF
TATARLRGRSVGSISGRTNAEVRLPLNVLELWSPDSPTLYDLEIALKSGDAVKSYFGMRSVEMRADAEGHNRIFLNHQPL
FQIGPLDQGWWPDGLYTAPTDEALRFDIEALKKMGFNMARKHVKVEPARWYYWCDKLGFLVWQDMPSAMTKTPPSNVRKG
AAEDAQFSAGEAAAWERELKAIIAANGNSPSIIVWVPFNEGWGQHATNDVLKMVKTLDPSRLVDGPSGWEDRGWGDLKDM
HNYPGPGMFPALNDRASVLGEFGGLGLPLAGHLWWDKRNWGYKTFEDRAQLQAAYEGLMDGLGPLVKNGLAAAIYTQTTD
VEGEINGLMTYDRQVMKFDVGKLAEMHRRLILGK

Sequences:

>Translated_594_residues
MRIQTAALIFWASASSLFAADWKVADNPLSTPWTSKVTAEPALPEYPRPQLERAKWTNLNGLWDYAIRAKGEGRPGQSEG
KLLVPFPVESALSGVKRPLTPDQRLWYSRSFNASAAKGSRLLLHFGAVDWRAEVFVNGKAVGTHEGGYDPFTFDITDALK
AGGAQEVTVAVWDPTDTALQPRGKQVLSPNGIWYTAVSGIWQTVWIEPVPAVYIESIAMTPDIDAHQLRLTVRSAASGDF
TATARLRGRSVGSISGRTNAEVRLPLNVLELWSPDSPTLYDLEIALKSGDAVKSYFGMRSVEMRADAEGHNRIFLNHQPL
FQIGPLDQGWWPDGLYTAPTDEALRFDIEALKKMGFNMARKHVKVEPARWYYWCDKLGFLVWQDMPSAMTKTPPSNVRKG
AAEDAQFSAGEAAAWERELKAIIAANGNSPSIIVWVPFNEGWGQHATNDVLKMVKTLDPSRLVDGPSGWEDRGWGDLKDM
HNYPGPGMFPALNDRASVLGEFGGLGLPLAGHLWWDKRNWGYKTFEDRAQLQAAYEGLMDGLGPLVKNGLAAAIYTQTTD
VEGEINGLMTYDRQVMKFDVGKLAEMHRRLILGK
>Mature_594_residues
MRIQTAALIFWASASSLFAADWKVADNPLSTPWTSKVTAEPALPEYPRPQLERAKWTNLNGLWDYAIRAKGEGRPGQSEG
KLLVPFPVESALSGVKRPLTPDQRLWYSRSFNASAAKGSRLLLHFGAVDWRAEVFVNGKAVGTHEGGYDPFTFDITDALK
AGGAQEVTVAVWDPTDTALQPRGKQVLSPNGIWYTAVSGIWQTVWIEPVPAVYIESIAMTPDIDAHQLRLTVRSAASGDF
TATARLRGRSVGSISGRTNAEVRLPLNVLELWSPDSPTLYDLEIALKSGDAVKSYFGMRSVEMRADAEGHNRIFLNHQPL
FQIGPLDQGWWPDGLYTAPTDEALRFDIEALKKMGFNMARKHVKVEPARWYYWCDKLGFLVWQDMPSAMTKTPPSNVRKG
AAEDAQFSAGEAAAWERELKAIIAANGNSPSIIVWVPFNEGWGQHATNDVLKMVKTLDPSRLVDGPSGWEDRGWGDLKDM
HNYPGPGMFPALNDRASVLGEFGGLGLPLAGHLWWDKRNWGYKTFEDRAQLQAAYEGLMDGLGPLVKNGLAAAIYTQTTD
VEGEINGLMTYDRQVMKFDVGKLAEMHRRLILGK

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787903, Length=357, Percent_Identity=24.3697478991597, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17510825, Length=419, Percent_Identity=24.1050119331742, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62471735, Length=469, Percent_Identity=22.8144989339019, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19922582, Length=469, Percent_Identity=22.8144989339019, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24655438, Length=469, Percent_Identity=22.8144989339019, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 65759; Mature: 65759

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQTAALIFWASASSLFAADWKVADNPLSTPWTSKVTAEPALPEYPRPQLERAKWTNLN
CCEEEEEEEEEECCCCEEEECCEECCCCCCCCCCCCEECCCCCCCCCCCHHHHCCCCCCC
GLWDYAIRAKGEGRPGQSEGKLLVPFPVESALSGVKRPLTPDQRLWYSRSFNASAAKGSR
CEEEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHEEEECCCCCCCCCCCE
LLLHFGAVDWRAEVFVNGKAVGTHEGGYDPFTFDITDALKAGGAQEVTVAVWDPTDTALQ
EEEEECCCCEEEEEEECCEEEECCCCCCCCEEEEHHHHHHCCCCCEEEEEEECCCCCCCC
PRGKQVLSPNGIWYTAVSGIWQTVWIEPVPAVYIESIAMTPDIDAHQLRLTVRSAASGDF
CCCCEEECCCCEEEEEHHHHHHEEECCCCCHHHHHHEECCCCCCCCEEEEEEECCCCCCE
TATARLRGRSVGSISGRTNAEVRLPLNVLELWSPDSPTLYDLEIALKSGDAVKSYFGMRS
EEEEEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHCCCE
VEMRADAEGHNRIFLNHQPLFQIGPLDQGWWPDGLYTAPTDEALRFDIEALKKMGFNMAR
EEEECCCCCCCEEEECCCCCEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCHHHHH
KHVKVEPARWYYWCDKLGFLVWQDMPSAMTKTPPSNVRKGAAEDAQFSAGEAAAWERELK
HHEECCCCCEEEEHHHCCEEEECCCCHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHEE
AIIAANGNSPSIIVWVPFNEGWGQHATNDVLKMVKTLDPSRLVDGPSGWEDRGWGDLKDM
EEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHH
HNYPGPGMFPALNDRASVLGEFGGLGLPLAGHLWWDKRNWGYKTFEDRAQLQAAYEGLMD
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
GLGPLVKNGLAAAIYTQTTDVEGEINGLMTYDRQVMKFDVGKLAEMHRRLILGK
HHHHHHHCCCEEEEEEECCCCCCCCCCEEHHCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIQTAALIFWASASSLFAADWKVADNPLSTPWTSKVTAEPALPEYPRPQLERAKWTNLN
CCEEEEEEEEEECCCCEEEECCEECCCCCCCCCCCCEECCCCCCCCCCCHHHHCCCCCCC
GLWDYAIRAKGEGRPGQSEGKLLVPFPVESALSGVKRPLTPDQRLWYSRSFNASAAKGSR
CEEEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHEEEECCCCCCCCCCCE
LLLHFGAVDWRAEVFVNGKAVGTHEGGYDPFTFDITDALKAGGAQEVTVAVWDPTDTALQ
EEEEECCCCEEEEEEECCEEEECCCCCCCCEEEEHHHHHHCCCCCEEEEEEECCCCCCCC
PRGKQVLSPNGIWYTAVSGIWQTVWIEPVPAVYIESIAMTPDIDAHQLRLTVRSAASGDF
CCCCEEECCCCEEEEEHHHHHHEEECCCCCHHHHHHEECCCCCCCCEEEEEEECCCCCCE
TATARLRGRSVGSISGRTNAEVRLPLNVLELWSPDSPTLYDLEIALKSGDAVKSYFGMRS
EEEEEECCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHCCCE
VEMRADAEGHNRIFLNHQPLFQIGPLDQGWWPDGLYTAPTDEALRFDIEALKKMGFNMAR
EEEECCCCCCCEEEECCCCCEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCHHHHH
KHVKVEPARWYYWCDKLGFLVWQDMPSAMTKTPPSNVRKGAAEDAQFSAGEAAAWERELK
HHEECCCCCEEEEHHHCCEEEECCCCHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHEE
AIIAANGNSPSIIVWVPFNEGWGQHATNDVLKMVKTLDPSRLVDGPSGWEDRGWGDLKDM
EEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHH
HNYPGPGMFPALNDRASVLGEFGGLGLPLAGHLWWDKRNWGYKTFEDRAQLQAAYEGLMD
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
GLGPLVKNGLAAAIYTQTTDVEGEINGLMTYDRQVMKFDVGKLAEMHRRLILGK
HHHHHHHCCCEEEEEEECCCCCCCCCCEEHHCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA