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The map label for this gene is asnA [H]

Identifier: 116625617

GI number: 116625617

Start: 8226711

End: 8227841

Strand: Reverse

Name: asnA [H]

Synonym: Acid_6566

Alternate gene names: 116625617

Gene position: 8227841-8226711 (Counterclockwise)

Preceding gene: 116625619

Following gene: 116625616

Centisome position: 82.56

GC content: 58.8

Gene sequence:

>1131_bases
ATGCTCGACAAGAAAGCGGACCTGGCCGGTCCCGGCATTGGCGACTATGAGGAACTGGAGAAAATCCTGCCCCAGGATTA
CTCCTCCCTGCTCTCCATCAGGGACACCCAGCGCGCCATCTTCGCAGGAAAGAACTTTATCGAGGAGCACCTTTGTCAGG
CGCTCAACCTCATGATGGTCACCGTACCGCTCGTCGTGGATACCGAGAGCGGGTTGAACGACCTATTGGATCGCGACGGC
TCCCGTACTCCGATCCAGTTCCACATCGCCAACGATCACGACAAGCATCCCATCGACGCGCAGATCGTGCAGGCCGCCAC
CAAGTGGAAACGCCTCGCCCTGCAGCGCTTCGGCATGAAGGCGGGCGAAGGCCTCTGCACCGATATGCGCGCCATCCGCA
AGGACTATTTTCTCGACCACGACCACAGCGCTTATGTTGACCAGTGGGATTGGGAGAAGGTCATTCATTTCTACGAACAC
GACCTCGACTTTTTGAAGCAGGCGGTCAACGCAATTTGGAAGGTGCTGGTCGATGCTGAAAGCTACCTGCAGAAGGAGTT
CCCCGCGCTCAAGGATTCGCGGTATCCGAACCTGCCGCGCAAACTCACGTTCCTTCATGCCGAAGACGTGCTCGACATGT
ACCCCGACCTGCCGCGCAAGCAACGCGAGACAAGATTGCTACAGGAGTGTCCCGCCGTCTTCCTGATCGGCATCGGCTGG
ACGCTGAAGGATGGCTATCCGCACGAAATGCGCGCCGCCGATTACGACGATTGGTGCACCGAGACGCGCTCCATCGACGG
CCGCCCCTGCCACGGTCTCAATGGCGACATTTTGGTGTGGAATCACGTGACCCGGCGCCGCCATGAGCTGAGCTCGATGG
GCATTCGCGTGAACGCGGAGACGCTGCAGAAGCAGTTGCGCATTACCAATCAACTGGACTTCCTGCGGTATCCGTATCAC
CAGGCCGTGGTTCGCCATGAACTGCCGGTTTCCATCGGCGGCGGCATCGGCCAATCGCGCACCTTGATGCTCCTGCTGCG
GAAAGCGCACTTGGGCGAAGTCACCGTGAGTGTATGGCCGAAGATCCTCAAGGAGATCTGCGCGGCGAAGAATATTCACG
TACTCGAGTAA

Upstream 100 bases:

>100_bases
ACGGGGGAACAACATAGAGGACTACCCACCAGTGTACCGTTCTTCCCCTATTCTCCACCTCTGAGTGGGTGTATAGTATT
TTCGGAGGTAAAACTCTTCC

Downstream 100 bases:

>100_bases
TTTGTGTATACTGCCTCCCATGAGCACTTCGAGGCGGAGATTCCTGCAGACGGCGTGTGCGGCGGCCCCGGTAATGTTGG
GCGCCACCGACAAAACGGGG

Product: asparagine synthetase AsnA

Products: NA

Alternate protein names: Asparagine synthetase A [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLDKKADLAGPGIGDYEELEKILPQDYSSLLSIRDTQRAIFAGKNFIEEHLCQALNLMMVTVPLVVDTESGLNDLLDRDG
SRTPIQFHIANDHDKHPIDAQIVQAATKWKRLALQRFGMKAGEGLCTDMRAIRKDYFLDHDHSAYVDQWDWEKVIHFYEH
DLDFLKQAVNAIWKVLVDAESYLQKEFPALKDSRYPNLPRKLTFLHAEDVLDMYPDLPRKQRETRLLQECPAVFLIGIGW
TLKDGYPHEMRAADYDDWCTETRSIDGRPCHGLNGDILVWNHVTRRRHELSSMGIRVNAETLQKQLRITNQLDFLRYPYH
QAVVRHELPVSIGGGIGQSRTLMLLLRKAHLGEVTVSVWPKILKEICAAKNIHVLE

Sequences:

>Translated_376_residues
MLDKKADLAGPGIGDYEELEKILPQDYSSLLSIRDTQRAIFAGKNFIEEHLCQALNLMMVTVPLVVDTESGLNDLLDRDG
SRTPIQFHIANDHDKHPIDAQIVQAATKWKRLALQRFGMKAGEGLCTDMRAIRKDYFLDHDHSAYVDQWDWEKVIHFYEH
DLDFLKQAVNAIWKVLVDAESYLQKEFPALKDSRYPNLPRKLTFLHAEDVLDMYPDLPRKQRETRLLQECPAVFLIGIGW
TLKDGYPHEMRAADYDDWCTETRSIDGRPCHGLNGDILVWNHVTRRRHELSSMGIRVNAETLQKQLRITNQLDFLRYPYH
QAVVRHELPVSIGGGIGQSRTLMLLLRKAHLGEVTVSVWPKILKEICAAKNIHVLE
>Mature_376_residues
MLDKKADLAGPGIGDYEELEKILPQDYSSLLSIRDTQRAIFAGKNFIEEHLCQALNLMMVTVPLVVDTESGLNDLLDRDG
SRTPIQFHIANDHDKHPIDAQIVQAATKWKRLALQRFGMKAGEGLCTDMRAIRKDYFLDHDHSAYVDQWDWEKVIHFYEH
DLDFLKQAVNAIWKVLVDAESYLQKEFPALKDSRYPNLPRKLTFLHAEDVLDMYPDLPRKQRETRLLQECPAVFLIGIGW
TLKDGYPHEMRAADYDDWCTETRSIDGRPCHGLNGDILVWNHVTRRRHELSSMGIRVNAETLQKQLRITNQLDFLRYPYH
QAVVRHELPVSIGGGIGQSRTLMLLLRKAHLGEVTVSVWPKILKEICAAKNIHVLE

Specific function: Asparagine biosynthesis. [C]

COG id: COG2502

COG function: function code E; Asparagine synthetase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790183, Length=332, Percent_Identity=42.7710843373494, Blast_Score=238, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004618 [H]

Pfam domain/function: PF03590 AsnA [H]

EC number: =6.3.1.1 [H]

Molecular weight: Translated: 43323; Mature: 43323

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKKADLAGPGIGDYEELEKILPQDYSSLLSIRDTQRAIFAGKNFIEEHLCQALNLMMV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVPLVVDTESGLNDLLDRDGSRTPIQFHIANDHDKHPIDAQIVQAATKWKRLALQRFGMK
HHEEEEECCCCHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
AGEGLCTDMRAIRKDYFLDHDHSAYVDQWDWEKVIHFYEHDLDFLKQAVNAIWKVLVDAE
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYLQKEFPALKDSRYPNLPRKLTFLHAEDVLDMYPDLPRKQRETRLLQECPAVFLIGIGW
HHHHHHCCCCCCCCCCCCCHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCC
TLKDGYPHEMRAADYDDWCTETRSIDGRPCHGLNGDILVWNHVTRRRHELSSMGIRVNAE
EECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCEEEHH
TLQKQLRITNQLDFLRYPYHQAVVRHELPVSIGGGIGQSRTLMLLLRKAHLGEVTVSVWP
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHH
KILKEICAAKNIHVLE
HHHHHHHCCCCCEECC
>Mature Secondary Structure
MLDKKADLAGPGIGDYEELEKILPQDYSSLLSIRDTQRAIFAGKNFIEEHLCQALNLMMV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVPLVVDTESGLNDLLDRDGSRTPIQFHIANDHDKHPIDAQIVQAATKWKRLALQRFGMK
HHEEEEECCCCHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
AGEGLCTDMRAIRKDYFLDHDHSAYVDQWDWEKVIHFYEHDLDFLKQAVNAIWKVLVDAE
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYLQKEFPALKDSRYPNLPRKLTFLHAEDVLDMYPDLPRKQRETRLLQECPAVFLIGIGW
HHHHHHCCCCCCCCCCCCCHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEEECCC
TLKDGYPHEMRAADYDDWCTETRSIDGRPCHGLNGDILVWNHVTRRRHELSSMGIRVNAE
EECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCEEEHH
TLQKQLRITNQLDFLRYPYHQAVVRHELPVSIGGGIGQSRTLMLLLRKAHLGEVTVSVWP
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHHH
KILKEICAAKNIHVLE
HHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA