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The map label for this gene is cysN [H]

Identifier: 116625566

GI number: 116625566

Start: 8163590

End: 8165266

Strand: Direct

Name: cysN [H]

Synonym: Acid_6513

Alternate gene names: 116625566

Gene position: 8163590-8165266 (Clockwise)

Preceding gene: 116625565

Following gene: 116625567

Centisome position: 81.92

GC content: 63.86

Gene sequence:

>1677_bases
ATGATGGCTCAACGTGTCGGTGCCGAAACCGCCGAGTGGCTCGCCGCCACCGCCGAAACCGAACTCCTCCGCTTCAGCAC
CGCCGGCAGTGTGGATGATGGCAAGAGCACCCTCATCGGCCGGCTGCTCTACGATTCCAAGGGCGTCTACGAAGACCAGC
TCGCCAGCGTCCGCAAGGCGACCCGCAATCTCTCCACCAACGGCCTCGATCTCTCCCTGCTCACCGATGGCCTCCGCGCC
GAGCGCGAACAGGGCATCACGATCGACGTCGCCTACCGCTATTTCTCCACCCCGCGCCGCAAGTTCATCATCGCCGACAC
TCCCGGCCACGAGCAGTACACCCGCAACATGGCCACCGGCGCTTCCACCGCCAATCTCTCCATCATCCTGATCGACGCGC
GCTACGGCGTGCTGCCCCAATCGCGCCGCCACGCCTACATCGCGGCCCTCTTGGGCATCCCCCACTTGGTCGTCGCCATC
AACAAGATGGACCTGGTGGAGTTTAGTCAGCAGACCTACAACACCATCCGCGAGAACTTCCGCGCCTTCGCCGCGCAGTT
AAATACCGGCGATATCACCTACATCCCGATCAGTGCGCTCGAAGGCGACAACGTTGTCACCCGCAGCCAGCGTACTCCGT
GGTACACCGGCCCCGCGCTCCTCGAACACCTCGAAACCGTTCCGATCGCGCGCGACTACAACCTGACCGATCTGCGATTC
CCGGTCCAGTATGTGATTCGCCCGAATCTGGATTTCCGCGGCTTCGCCGGACAGATTGCTTCCGGCGTGATCCGCCGCGG
CGATCCCGTCACGGTGCTCCCTTCCGGCCGCGCCAGCCGCGTCAAATCCATCGTCACCTGGGAAGGCGACCTGGCCGAGG
CGTTCGCGCCCATGTCGGTCACCGTCTGCCTTGAAGACGAAATCGATATCAGCCGCGGCGACATGCTCGCCCTCCCCGAA
AACCTGCCTCACTCAGGCCGCCGCTTCGATGCCACTGTGGTCTGGATGAACCAGAAGCCGCTCGCGCCCAATCGCCCCTA
CCTCATCAAGCAGACCACCCAGGTCACGCAGGCCCGCATTCGCCGGATCCACCACCGCATCGATATTCACACGCTGGAAC
ACCAGCAGGTCGGCGAACTCGAACTCAATGGCATCGGCGTGGTGAGCGTGGAGGCCCAGCGCCCCCTCTTCTTCGACCCC
TACCGCACCAACCGCGCCACCGGCAGTTTCATCCTGATCGACCCGATCACTAATGAGACTCTCGGCGCCGGCATGATTCT
CGAGCCGCATTCCGGCTCGGGCGGCACGGGACGCGTCACCGACGCCGAACGCCGCGCTGCCCGCGGCCACGCCCCGCTCG
TCATCTGCTTGCCCCAGGGCAATACCGAACTTCCCTGGCTGCTCGAGCGCCTGCTCTTCGACCACGGCTACGCAGTCCAC
GTAATCGATCGGGCCGTTGATCTCGCCGAGGCCATCCGCACCACCCTGGCCGCTGGGCTGGTTGCGATCGTCCAGCCCTC
TAACGCGCAGCAGTCGCAGCTCGTGAAAGATTCCGTGCCTCTCTCGCTTCGTTATCTTCCTGCATCGAACGATCACGAGC
AGATATGGTCGGAATTGGAGACGTCAGGATGCCTCGGGCATTCCCCTGACCCGCTAACCGATGGTGCGGGGATTTAG

Upstream 100 bases:

>100_bases
AAGAGATGATTCAGGCGCGCCGCAGCGAGCGCGAAAATCGCATCATCGACCACGACCAGGAAGGTTCCATGGAGATCAAG
AAGCGCGAGGGCTACTTCTG

Downstream 100 bases:

>100_bases
TTACTTAAGTAAGAATTTCCCCTACAGAATATCCCCCACTCTGTAACCTGCAACGATCCCCTACATAGTAACCTTCGATA
GCCCCCAGTACCTTAGGTAC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MMAQRVGAETAEWLAATAETELLRFSTAGSVDDGKSTLIGRLLYDSKGVYEDQLASVRKATRNLSTNGLDLSLLTDGLRA
EREQGITIDVAYRYFSTPRRKFIIADTPGHEQYTRNMATGASTANLSIILIDARYGVLPQSRRHAYIAALLGIPHLVVAI
NKMDLVEFSQQTYNTIRENFRAFAAQLNTGDITYIPISALEGDNVVTRSQRTPWYTGPALLEHLETVPIARDYNLTDLRF
PVQYVIRPNLDFRGFAGQIASGVIRRGDPVTVLPSGRASRVKSIVTWEGDLAEAFAPMSVTVCLEDEIDISRGDMLALPE
NLPHSGRRFDATVVWMNQKPLAPNRPYLIKQTTQVTQARIRRIHHRIDIHTLEHQQVGELELNGIGVVSVEAQRPLFFDP
YRTNRATGSFILIDPITNETLGAGMILEPHSGSGGTGRVTDAERRAARGHAPLVICLPQGNTELPWLLERLLFDHGYAVH
VIDRAVDLAEAIRTTLAAGLVAIVQPSNAQQSQLVKDSVPLSLRYLPASNDHEQIWSELETSGCLGHSPDPLTDGAGI

Sequences:

>Translated_558_residues
MMAQRVGAETAEWLAATAETELLRFSTAGSVDDGKSTLIGRLLYDSKGVYEDQLASVRKATRNLSTNGLDLSLLTDGLRA
EREQGITIDVAYRYFSTPRRKFIIADTPGHEQYTRNMATGASTANLSIILIDARYGVLPQSRRHAYIAALLGIPHLVVAI
NKMDLVEFSQQTYNTIRENFRAFAAQLNTGDITYIPISALEGDNVVTRSQRTPWYTGPALLEHLETVPIARDYNLTDLRF
PVQYVIRPNLDFRGFAGQIASGVIRRGDPVTVLPSGRASRVKSIVTWEGDLAEAFAPMSVTVCLEDEIDISRGDMLALPE
NLPHSGRRFDATVVWMNQKPLAPNRPYLIKQTTQVTQARIRRIHHRIDIHTLEHQQVGELELNGIGVVSVEAQRPLFFDP
YRTNRATGSFILIDPITNETLGAGMILEPHSGSGGTGRVTDAERRAARGHAPLVICLPQGNTELPWLLERLLFDHGYAVH
VIDRAVDLAEAIRTTLAAGLVAIVQPSNAQQSQLVKDSVPLSLRYLPASNDHEQIWSELETSGCLGHSPDPLTDGAGI
>Mature_558_residues
MMAQRVGAETAEWLAATAETELLRFSTAGSVDDGKSTLIGRLLYDSKGVYEDQLASVRKATRNLSTNGLDLSLLTDGLRA
EREQGITIDVAYRYFSTPRRKFIIADTPGHEQYTRNMATGASTANLSIILIDARYGVLPQSRRHAYIAALLGIPHLVVAI
NKMDLVEFSQQTYNTIRENFRAFAAQLNTGDITYIPISALEGDNVVTRSQRTPWYTGPALLEHLETVPIARDYNLTDLRF
PVQYVIRPNLDFRGFAGQIASGVIRRGDPVTVLPSGRASRVKSIVTWEGDLAEAFAPMSVTVCLEDEIDISRGDMLALPE
NLPHSGRRFDATVVWMNQKPLAPNRPYLIKQTTQVTQARIRRIHHRIDIHTLEHQQVGELELNGIGVVSVEAQRPLFFDP
YRTNRATGSFILIDPITNETLGAGMILEPHSGSGGTGRVTDAERRAARGHAPLVICLPQGNTELPWLLERLLFDHGYAVH
VIDRAVDLAEAIRTTLAAGLVAIVQPSNAQQSQLVKDSVPLSLRYLPASNDHEQIWSELETSGCLGHSPDPLTDGAGI

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=425, Percent_Identity=28.2352941176471, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI5729864, Length=425, Percent_Identity=28.2352941176471, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=27.0509977827051, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=27.0509977827051, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI46094014, Length=329, Percent_Identity=31.0030395136778, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI194018522, Length=329, Percent_Identity=31.0030395136778, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI194097354, Length=329, Percent_Identity=31.0030395136778, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI194018520, Length=329, Percent_Identity=31.0030395136778, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI34147630, Length=344, Percent_Identity=27.3255813953488, Blast_Score=93, Evalue=8e-19,
Organism=Escherichia coli, GI1789108, Length=436, Percent_Identity=56.8807339449541, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1790412, Length=339, Percent_Identity=25.3687315634218, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789737, Length=339, Percent_Identity=25.3687315634218, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367247, Length=300, Percent_Identity=24, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=27.9379157427938, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=27.9379157427938, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32566629, Length=429, Percent_Identity=26.3403263403263, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=442, Percent_Identity=26.6968325791855, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=281, Percent_Identity=30.2491103202847, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=281, Percent_Identity=30.2491103202847, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI32566301, Length=136, Percent_Identity=34.5588235294118, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25141371, Length=209, Percent_Identity=27.7511961722488, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=285, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=334, Percent_Identity=30.5389221556886, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=334, Percent_Identity=30.5389221556886, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=380, Percent_Identity=30, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=425, Percent_Identity=29.1764705882353, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324761, Length=317, Percent_Identity=27.1293375394322, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24652838, Length=452, Percent_Identity=26.3274336283186, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137572, Length=452, Percent_Identity=26.3274336283186, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45550900, Length=421, Percent_Identity=27.790973871734, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=26.0674157303371, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=26.0674157303371, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=26.0674157303371, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=26.0674157303371, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137380, Length=419, Percent_Identity=25.7756563245823, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI281363316, Length=249, Percent_Identity=28.5140562248996, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI17864358, Length=249, Percent_Identity=28.5140562248996, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19921738, Length=144, Percent_Identity=36.1111111111111, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 61493; Mature: 61493

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAQRVGAETAEWLAATAETELLRFSTAGSVDDGKSTLIGRLLYDSKGVYEDQLASVRKA
CCCCHHCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
TRNLSTNGLDLSLLTDGLRAEREQGITIDVAYRYFSTPRRKFIIADTPGHEQYTRNMATG
HHCCCCCCEEEEEECCCHHHHHHCCCEEEEEEHHHCCCCEEEEEEECCCCHHHHHHHHCC
ASTANLSIILIDARYGVLPQSRRHAYIAALLGIPHLVVAINKMDLVEFSQQTYNTIRENF
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHH
RAFAAQLNTGDITYIPISALEGDNVVTRSQRTPWYTGPALLEHLETVPIARDYNLTDLRF
HHHHHCCCCCCEEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEC
PVQYVIRPNLDFRGFAGQIASGVIRRGDPVTVLPSGRASRVKSIVTWEGDLAEAFAPMSV
CEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHCCEEEEECCCHHHHCCCEEE
TVCLEDEIDISRGDMLALPENLPHSGRRFDATVVWMNQKPLAPNRPYLIKQTTQVTQARI
EEEEECCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEECHHHHHHHHH
RRIHHRIDIHTLEHQQVGELELNGIGVVSVEAQRPLFFDPYRTNRATGSFILIDPITNET
HHHHHHHEEEEECCCCCCCEEECCEEEEEEECCCCEEECCCCCCCCCCCEEEEECCCCCC
LGAGMILEPHSGSGGTGRVTDAERRAARGHAPLVICLPQGNTELPWLLERLLFDHGYAVH
CCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEE
VIDRAVDLAEAIRTTLAAGLVAIVQPSNAQQSQLVKDSVPLSLRYLPASNDHEQIWSELE
EHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
TSGCLGHSPDPLTDGAGI
HCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMAQRVGAETAEWLAATAETELLRFSTAGSVDDGKSTLIGRLLYDSKGVYEDQLASVRKA
CCCCHHCCCHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
TRNLSTNGLDLSLLTDGLRAEREQGITIDVAYRYFSTPRRKFIIADTPGHEQYTRNMATG
HHCCCCCCEEEEEECCCHHHHHHCCCEEEEEEHHHCCCCEEEEEEECCCCHHHHHHHHCC
ASTANLSIILIDARYGVLPQSRRHAYIAALLGIPHLVVAINKMDLVEFSQQTYNTIRENF
CCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHH
RAFAAQLNTGDITYIPISALEGDNVVTRSQRTPWYTGPALLEHLETVPIARDYNLTDLRF
HHHHHCCCCCCEEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEC
PVQYVIRPNLDFRGFAGQIASGVIRRGDPVTVLPSGRASRVKSIVTWEGDLAEAFAPMSV
CEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHCCEEEEECCCHHHHCCCEEE
TVCLEDEIDISRGDMLALPENLPHSGRRFDATVVWMNQKPLAPNRPYLIKQTTQVTQARI
EEEEECCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEECHHHHHHHHH
RRIHHRIDIHTLEHQQVGELELNGIGVVSVEAQRPLFFDPYRTNRATGSFILIDPITNET
HHHHHHHEEEEECCCCCCCEEECCEEEEEEECCCCEEECCCCCCCCCCCEEEEECCCCCC
LGAGMILEPHSGSGGTGRVTDAERRAARGHAPLVICLPQGNTELPWLLERLLFDHGYAVH
CCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEE
VIDRAVDLAEAIRTTLAAGLVAIVQPSNAQQSQLVKDSVPLSLRYLPASNDHEQIWSELE
EHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
TSGCLGHSPDPLTDGAGI
HCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA