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The map label for this gene is cysD [H]

Identifier: 116625565

GI number: 116625565

Start: 8162688

End: 8163590

Strand: Direct

Name: cysD [H]

Synonym: Acid_6512

Alternate gene names: 116625565

Gene position: 8162688-8163590 (Clockwise)

Preceding gene: 116625562

Following gene: 116625566

Centisome position: 81.91

GC content: 61.79

Gene sequence:

>903_bases
ATGGATCGGACCCTGAGCCATCTTCGCGCCCTGGAGGCCGAAAGCATTCACATCATCCGCGAGGTCGCCGCGGAATTTGA
ACGCCCTGTCATGTTGTATTCCATCGGCAAGGATTCGTCCGTGATGGTGCGCCTCGCCCAGAAGGCCTTCGCCCCCGGCC
CCTTGCCGTTTCCCCTCCTGCACATCGATACCACCTACAAATTCGACGAAATGATCGAGTTCCGCGACCGCTTCTGCGCG
GAAATCGGCGCCCGCCTGATCGTCCACACCAATCGCAAAGCCATCGAAGACGGCGCCAGTCCCTTCAAGCTGGGCACCCA
GAAATGCTGCGGCCTGCTCAAAACCCGCGCCCTGCTGGACGCGCTCGCCGAGGGCAACTTCGACGCGGCCTTCGGCGGCG
CCCGGCGCGACGAAGAGAAGTCGCGCGCCAAGGAACGCGTCTACAGCTTCCGCGACGCCTTCGGCCAGTGGGACCCCAAG
AATCAGCGCCCCGAACTCTGGAACCTCTACAACAGCCGCATCGATAAGGGCGAGACCATCCGCGTCTTTCCGCTTTCCAA
CTGGACCGAGCTCGACGTCTGGAACTATATCTATCGCGAGAATATCCCGATCGTACCGCTCTATTTCGCCAAGCCCCGTC
CCATGCTGGTGCGCGGCGGCAACTTGATCCCGCTCGAACACAACGCGCCCCTGCTCCCCGGCGAGCAGCCCGAAATGGTG
ATGTGCCGCATGCGCTCCCTCGGCTGCACCTACTGCACCGGCGCCATTTATTCCGAAGCCGACACCCTGCCCAAGATCAT
CGAAGAGATGATTCAGGCGCGCCGCAGCGAGCGCGAAAATCGCATCATCGACCACGACCAGGAAGGTTCCATGGAGATCA
AGAAGCGCGAGGGCTACTTCTGA

Upstream 100 bases:

>100_bases
GCCCAACGAGGCAATCACCAGTAACCCTATAGACTTCAACATGATTGGATGGTATCCTAAGACGAACGTAACATAGCGAA
ACCGTCCTCAAATCAATCCC

Downstream 100 bases:

>100_bases
TGATGGCTCAACGTGTCGGTGCCGAAACCGCCGAGTGGCTCGCCGCCACCGCCGAAACCGAACTCCTCCGCTTCAGCACC
GCCGGCAGTGTGGATGATGG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDRTLSHLRALEAESIHIIREVAAEFERPVMLYSIGKDSSVMVRLAQKAFAPGPLPFPLLHIDTTYKFDEMIEFRDRFCA
EIGARLIVHTNRKAIEDGASPFKLGTQKCCGLLKTRALLDALAEGNFDAAFGGARRDEEKSRAKERVYSFRDAFGQWDPK
NQRPELWNLYNSRIDKGETIRVFPLSNWTELDVWNYIYRENIPIVPLYFAKPRPMLVRGGNLIPLEHNAPLLPGEQPEMV
MCRMRSLGCTYCTGAIYSEADTLPKIIEEMIQARRSERENRIIDHDQEGSMEIKKREGYF

Sequences:

>Translated_300_residues
MDRTLSHLRALEAESIHIIREVAAEFERPVMLYSIGKDSSVMVRLAQKAFAPGPLPFPLLHIDTTYKFDEMIEFRDRFCA
EIGARLIVHTNRKAIEDGASPFKLGTQKCCGLLKTRALLDALAEGNFDAAFGGARRDEEKSRAKERVYSFRDAFGQWDPK
NQRPELWNLYNSRIDKGETIRVFPLSNWTELDVWNYIYRENIPIVPLYFAKPRPMLVRGGNLIPLEHNAPLLPGEQPEMV
MCRMRSLGCTYCTGAIYSEADTLPKIIEEMIQARRSERENRIIDHDQEGSMEIKKREGYF
>Mature_300_residues
MDRTLSHLRALEAESIHIIREVAAEFERPVMLYSIGKDSSVMVRLAQKAFAPGPLPFPLLHIDTTYKFDEMIEFRDRFCA
EIGARLIVHTNRKAIEDGASPFKLGTQKCCGLLKTRALLDALAEGNFDAAFGGARRDEEKSRAKERVYSFRDAFGQWDPK
NQRPELWNLYNSRIDKGETIRVFPLSNWTELDVWNYIYRENIPIVPLYFAKPRPMLVRGGNLIPLEHNAPLLPGEQPEMV
MCRMRSLGCTYCTGAIYSEADTLPKIIEEMIQARRSERENRIIDHDQEGSMEIKKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=63.6363636363636, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34561; Mature: 34561

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRTLSHLRALEAESIHIIREVAAEFERPVMLYSIGKDSSVMVRLAQKAFAPGPLPFPLL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCEEE
HIDTTYKFDEMIEFRDRFCAEIGARLIVHTNRKAIEDGASPFKLGTQKCCGLLKTRALLD
EECCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ALAEGNFDAAFGGARRDEEKSRAKERVYSFRDAFGQWDPKNQRPELWNLYNSRIDKGETI
HHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEE
RVFPLSNWTELDVWNYIYRENIPIVPLYFAKPRPMLVRGGNLIPLEHNAPLLPGEQPEMV
EEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCEEEECCCCCCCCCCCHHHH
MCRMRSLGCTYCTGAIYSEADTLPKIIEEMIQARRSERENRIIDHDQEGSMEIKKREGYF
HHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHCCCCC
>Mature Secondary Structure
MDRTLSHLRALEAESIHIIREVAAEFERPVMLYSIGKDSSVMVRLAQKAFAPGPLPFPLL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCEEE
HIDTTYKFDEMIEFRDRFCAEIGARLIVHTNRKAIEDGASPFKLGTQKCCGLLKTRALLD
EECCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ALAEGNFDAAFGGARRDEEKSRAKERVYSFRDAFGQWDPKNQRPELWNLYNSRIDKGETI
HHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEE
RVFPLSNWTELDVWNYIYRENIPIVPLYFAKPRPMLVRGGNLIPLEHNAPLLPGEQPEMV
EEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCEEEECCCCCCCCCCCHHHH
MCRMRSLGCTYCTGAIYSEADTLPKIIEEMIQARRSERENRIIDHDQEGSMEIKKREGYF
HHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA