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The map label for this gene is nirA [H]

Identifier: 116625485

GI number: 116625485

Start: 8051613

End: 8053397

Strand: Reverse

Name: nirA [H]

Synonym: Acid_6431

Alternate gene names: 116625485

Gene position: 8053397-8051613 (Counterclockwise)

Preceding gene: 116625486

Following gene: 116625484

Centisome position: 80.81

GC content: 64.99

Gene sequence:

>1785_bases
ATGGCGACTTCCACCGAGCAAGCACTTTCTCAACGTGAACAGCTTGAGAATGAACACCTTAAAACCGTCCGCGAAGAAAT
CGAAATCTTCCGGACAAAAGCAATGTCCGTCCTGGCAGGGGAGATTACCGACGCGGAGTTCCGCCCCTTCCGGCTCAAGC
ACGGCATCTACGGGCAACGCCAGCCCGGGGTCCAAATGGTCCGCTGCAAAATCCCAAGCGGGTTATTGACCGCAGATCAG
GTGGGGCAACTCGCCCGCATCGCCGACGAATTCGGCGGCGGCCGCGGCCACCTCACCACCCGTCAGAACATCCAGTACCA
CTTCGTTCCCCTCGCGCAGGTCCCCGACCTCATGCACCTCCTGGCCGACGTCGGCCTCACCAATCGCGAAGCCTGCTACA
ACACCGTCCGCAACGTGACCGCCTGCCCCTGGGCCGGCATCGCGCAGGACGAAGTCTTTGACGTGCGCCCCTACGCGCAG
CGCCTGGCCTACGCCTTCCTCCGCAAAGACCTCACCGGCAACCTTCCGCGCAAGTTCAAATTCGCCTTCGATGGCTGCCG
CCACGAGGATTGCATCCAGGGCGCCATCAACGATGTCGGCCTGCGCGCCGTCGTCCGTGACGGCAAGCGCGGCTTCCGCA
TGGTCATCGGCGGCGGCCTCGGCCCCCTCCCCACCGAAGCCCAACTGCTCGAGGAGTTCGTCCCCGAGGAACAGCTGCTC
CACAAGTGCGAGGCCATCGTCCGCGTCTTCAACCAGTACGGCAACCGCAAGAACAAGAACACCGCGCGCATGAAGTTTGT
GATGCGCAATCGCGGCCTGGAATGGATGCGCGAGCAGATCGAGCTGGCCTACCAGGACATCCTCCTGAACGGCGGCATTG
CCTGGCCCGCGATCGTTCCCGAAGGCTTCGGCGGCTATGAATCCAACCCCCAGCCCCTCGGCAACGGCGCGCTCCTGCCG
GTGGTCAACCACTCCACCAGCGGAGACGCGGCCTACGATCGCTGGCTCGAGACCAACGTCCGCGAACAGAAGCAGACCGG
CTATGCCGCGGTCACGGTCACCGTGGATCAGGGCAACTTGACCGGCGATCAGCTGCGCGGCCTCGCCAAACTGGCCGGCA
CCGCCGGCGATGGCCTGCTGCGCACCGCCATCAATCAGAACGTGGTGCTCGGGTTCATCCCGCTGTCGCGCTTGCCGCAG
GTCTACCAGGCGCTGGGCGACCTGAATCTTGCCGGCGCCGGCGCGGGCCAGATCGAAGACGTCCTCACCTGCCCGGGCGC
CTACACCTGCAACCTCGGCATCACCAAGACCATGAACCTGGGCGCTGCGCTGCAGGAGACCGTGCGTCAGTTCGACGATC
CCGAAGTGCGCCGCCTGACCATCAAGGCCAGCGGCTGCCCCAACGCCTGCGGCCATCACTGGATCGGCGATTTCGGTTTC
TACGGCAACGCCCGCAAGCTCGAGGGCCGCGAGATCCCCTACTACCAGATGCTCCTCGGCGGCGGCTACGACGATCAGGG
CATCATGCGCTTCGGCCTCGCCATTCAATCGCTGCCCGCGCGCCTCGCTCCCGAAGCCGTGCGCCGCGTAATCCAGCACT
TCCAGGCCAACCGCCTGCGCGGCGAGCGCTTCCGCGACTACGTCCTGCGCTTCAAAATCGAGACCTTCCGCGAGCTCACC
GCCGACCTGGCCAAGCCCGCCGACCTCTTCCCCGAAATCTACCAGGATTGGGGCGACTCCGAAGCCTACTCCCTAAAACT
CGGCCGCGGCGAATGCGCCGTGTAA

Upstream 100 bases:

>100_bases
CCTGGCTCCCGCCCCTCTCCTTCATTTGCACTCCCCGGGGGAATTAGGGTAATCTCTATATATAATCAGAGGATTAATCC
CCATCGGGATGCAGGTGATT

Downstream 100 bases:

>100_bases
TCTCCCGCGCCGTGCGCCGGTCACCGCCCGCCTCCATCAGAGAGCGATCTTTACGAACGCGAACCGCGACCGTCAGGGAG
CGATCTACAATACTTTGATG

Product: nitrite/sulfite reductase hemoprotein subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MATSTEQALSQREQLENEHLKTVREEIEIFRTKAMSVLAGEITDAEFRPFRLKHGIYGQRQPGVQMVRCKIPSGLLTADQ
VGQLARIADEFGGGRGHLTTRQNIQYHFVPLAQVPDLMHLLADVGLTNREACYNTVRNVTACPWAGIAQDEVFDVRPYAQ
RLAYAFLRKDLTGNLPRKFKFAFDGCRHEDCIQGAINDVGLRAVVRDGKRGFRMVIGGGLGPLPTEAQLLEEFVPEEQLL
HKCEAIVRVFNQYGNRKNKNTARMKFVMRNRGLEWMREQIELAYQDILLNGGIAWPAIVPEGFGGYESNPQPLGNGALLP
VVNHSTSGDAAYDRWLETNVREQKQTGYAAVTVTVDQGNLTGDQLRGLAKLAGTAGDGLLRTAINQNVVLGFIPLSRLPQ
VYQALGDLNLAGAGAGQIEDVLTCPGAYTCNLGITKTMNLGAALQETVRQFDDPEVRRLTIKASGCPNACGHHWIGDFGF
YGNARKLEGREIPYYQMLLGGGYDDQGIMRFGLAIQSLPARLAPEAVRRVIQHFQANRLRGERFRDYVLRFKIETFRELT
ADLAKPADLFPEIYQDWGDSEAYSLKLGRGECAV

Sequences:

>Translated_594_residues
MATSTEQALSQREQLENEHLKTVREEIEIFRTKAMSVLAGEITDAEFRPFRLKHGIYGQRQPGVQMVRCKIPSGLLTADQ
VGQLARIADEFGGGRGHLTTRQNIQYHFVPLAQVPDLMHLLADVGLTNREACYNTVRNVTACPWAGIAQDEVFDVRPYAQ
RLAYAFLRKDLTGNLPRKFKFAFDGCRHEDCIQGAINDVGLRAVVRDGKRGFRMVIGGGLGPLPTEAQLLEEFVPEEQLL
HKCEAIVRVFNQYGNRKNKNTARMKFVMRNRGLEWMREQIELAYQDILLNGGIAWPAIVPEGFGGYESNPQPLGNGALLP
VVNHSTSGDAAYDRWLETNVREQKQTGYAAVTVTVDQGNLTGDQLRGLAKLAGTAGDGLLRTAINQNVVLGFIPLSRLPQ
VYQALGDLNLAGAGAGQIEDVLTCPGAYTCNLGITKTMNLGAALQETVRQFDDPEVRRLTIKASGCPNACGHHWIGDFGF
YGNARKLEGREIPYYQMLLGGGYDDQGIMRFGLAIQSLPARLAPEAVRRVIQHFQANRLRGERFRDYVLRFKIETFRELT
ADLAKPADLFPEIYQDWGDSEAYSLKLGRGECAV
>Mature_593_residues
ATSTEQALSQREQLENEHLKTVREEIEIFRTKAMSVLAGEITDAEFRPFRLKHGIYGQRQPGVQMVRCKIPSGLLTADQV
GQLARIADEFGGGRGHLTTRQNIQYHFVPLAQVPDLMHLLADVGLTNREACYNTVRNVTACPWAGIAQDEVFDVRPYAQR
LAYAFLRKDLTGNLPRKFKFAFDGCRHEDCIQGAINDVGLRAVVRDGKRGFRMVIGGGLGPLPTEAQLLEEFVPEEQLLH
KCEAIVRVFNQYGNRKNKNTARMKFVMRNRGLEWMREQIELAYQDILLNGGIAWPAIVPEGFGGYESNPQPLGNGALLPV
VNHSTSGDAAYDRWLETNVREQKQTGYAAVTVTVDQGNLTGDQLRGLAKLAGTAGDGLLRTAINQNVVLGFIPLSRLPQV
YQALGDLNLAGAGAGQIEDVLTCPGAYTCNLGITKTMNLGAALQETVRQFDDPEVRRLTIKASGCPNACGHHWIGDFGFY
GNARKLEGREIPYYQMLLGGGYDDQGIMRFGLAIQSLPARLAPEAVRRVIQHFQANRLRGERFRDYVLRFKIETFRELTA
DLAKPADLFPEIYQDWGDSEAYSLKLGRGECAV

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=526, Percent_Identity=24.1444866920152, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 66016; Mature: 65885

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSTEQALSQREQLENEHLKTVREEIEIFRTKAMSVLAGEITDAEFRPFRLKHGIYGQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCC
QPGVQMVRCKIPSGLLTADQVGQLARIADEFGGGRGHLTTRQNIQYHFVPLAQVPDLMHL
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHHHHHHHH
LADVGLTNREACYNTVRNVTACPWAGIAQDEVFDVRPYAQRLAYAFLRKDLTGNLPRKFK
HHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
FAFDGCRHEDCIQGAINDVGLRAVVRDGKRGFRMVIGGGLGPLPTEAQLLEEFVPEEQLL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHH
HKCEAIVRVFNQYGNRKNKNTARMKFVMRNRGLEWMREQIELAYQDILLNGGIAWPAIVP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGFGGYESNPQPLGNGALLPVVNHSTSGDAAYDRWLETNVREQKQTGYAAVTVTVDQGNL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
TGDQLRGLAKLAGTAGDGLLRTAINQNVVLGFIPLSRLPQVYQALGDLNLAGAGAGQIED
CHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHH
VLTCPGAYTCNLGITKTMNLGAALQETVRQFDDPEVRRLTIKASGCPNACGHHWIGDFGF
HHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
YGNARKLEGREIPYYQMLLGGGYDDQGIMRFGLAIQSLPARLAPEAVRRVIQHFQANRLR
CCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHC
GERFRDYVLRFKIETFRELTADLAKPADLFPEIYQDWGDSEAYSLKLGRGECAV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
ATSTEQALSQREQLENEHLKTVREEIEIFRTKAMSVLAGEITDAEFRPFRLKHGIYGQR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCC
QPGVQMVRCKIPSGLLTADQVGQLARIADEFGGGRGHLTTRQNIQYHFVPLAQVPDLMHL
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEHHHHHHHHHH
LADVGLTNREACYNTVRNVTACPWAGIAQDEVFDVRPYAQRLAYAFLRKDLTGNLPRKFK
HHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
FAFDGCRHEDCIQGAINDVGLRAVVRDGKRGFRMVIGGGLGPLPTEAQLLEEFVPEEQLL
HHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHH
HKCEAIVRVFNQYGNRKNKNTARMKFVMRNRGLEWMREQIELAYQDILLNGGIAWPAIVP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGFGGYESNPQPLGNGALLPVVNHSTSGDAAYDRWLETNVREQKQTGYAAVTVTVDQGNL
CCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
TGDQLRGLAKLAGTAGDGLLRTAINQNVVLGFIPLSRLPQVYQALGDLNLAGAGAGQIED
CHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHH
VLTCPGAYTCNLGITKTMNLGAALQETVRQFDDPEVRRLTIKASGCPNACGHHWIGDFGF
HHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
YGNARKLEGREIPYYQMLLGGGYDDQGIMRFGLAIQSLPARLAPEAVRRVIQHFQANRLR
CCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHC
GERFRDYVLRFKIETFRELTADLAKPADLFPEIYQDWGDSEAYSLKLGRGECAV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]