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The map label for this gene is purH [H]

Identifier: 116625484

GI number: 116625484

Start: 8049968

End: 8051497

Strand: Reverse

Name: purH [H]

Synonym: Acid_6430

Alternate gene names: 116625484

Gene position: 8051497-8049968 (Counterclockwise)

Preceding gene: 116625485

Following gene: 116625482

Centisome position: 80.79

GC content: 65.82

Gene sequence:

>1530_bases
ATGCCAAAAATCGCACGTGTACTGATTAGCGTTACCGACAAAACCGGGATCATCGACTTCGCGTCCGCCCTCTCCGCAAT
GGGCGCCGACCTGATCTCCACCGGTGGCACCGCCCGCATGATCCGCGAAGCCGGCATCCCCGTAGCCGACGTTAGCGACG
TCACCGGCTTCCCCGAGATGCTCGACGGCCGCGTCAAGACCATGCACCCGAAGATCGCCGGCGGCATCCTGGCGATGCGC
GGCAATCCCGAGCACATGGCCGCGATCTCCACTCACGGAATCGCACCCATTGACATGGTGGTGGTCAATCTCTACCGATT
CGAAGAAGTCGCAGCCAAGGCCGGCGCGCACCTCGAAGAGCTCATCGAGAACATCGATATCGGCGGCCCCACCATGCTCC
GCGCGGCCGCGAAAAACTATCAGGACGTCGCCGTCGTCGTCTCTCCCGCCGATTACCCGGCATTGATCGAGGAGATGCGC
GCCTCCGCCGGCGCACTCTCCCACGAAACCAAGTGGCGCCTGGCCAAAAAGGTCTTCCGCGCCACCGCGGATTACGACGC
CGCCATCAGCGCCCGCCTCGAACAAGTGGACGCCCCCGCCGCGCTCCTCCCACACTTGGCGATCCGCGCCGCCAAGCTGA
TGGACCTCCGCTACGGCGAAAATCCGCATCAATCCGCCGCCCTCTATGGCCACCGCGGCCAGGGCATCGCCGGCGCCGAG
CAGCTCCACGGCAAGGAGCTCAGCTACAACAACCTGGTGGACCTCGACGCCGCCTGGCAGCTCGTCAACGAATTCAACCG
GCCGGCCGTCGCCATCATCAAACACACCAACCCCTGCGGTTGCGCCGAGCAGGATACCCTCGCCGCCGCATACCGCAAGG
CCTTCGAAGCCGACCCTGTTTCGGCCTTCGGCGGCGTGATCGGCATCAATCGCACCGTGGACGAGGAAACCGCGCGCGAG
CTCGCCAAGACCTTCCTCGAAGCCATCGCCGCGCCTGACTTCACCGCCGAGGCGCTCTCCATCCTCACCGCGAAGAAGAA
CCTCCGTCTCATGCGCGTGGCCCCAGCCGCCGACCCCTTGGTGGTGAAATCGATCTCCGGCGGCTACCTCGCCCAGACCG
CCGACACCGCGAAGTTGGACCGCGCCCAGGCCGTCGTCAAAACCAGGCGCGCCCCGACCGAAGAGGAATGGGTGGCCCTG
GAGTTTGGCTGGAAGGTGGCCAAGCACGTCAAGTCGAACGCCATCGTCTATGCGCGCGCCGGCCAGACCGTCGGTGTCGG
AGCCGGCCAGATGAGCCGCGTCGATTCCGTCAAGTTAGGCGCCATGAAGGCCGTGCTCCCCGTGGCCGGCACCGTCGTTG
CATCGGACGCCTTCTTCCCGTTCCCCGATGGAGTCGAAGAGGCCGCTAAGAACGGCGCAACGGCATTCATCCAGCCCGGA
GGCTCCGTGCGCGACGAAGAAGTCATCGCCGCCGCCGACCGCCTCGGCGTCGCCATGGTCTTCACCGGCGTCCGCCACTT
CCGCCACTAA

Upstream 100 bases:

>100_bases
GCCGGTCACCGCCCGCCTCCATCAGAGAGCGATCTTTACGAACGCGAACCGCGACCGTCAGGGAGCGATCTACAATACTT
TGATGCTATCGTGCGAGTAG

Downstream 100 bases:

>100_bases
CGCCCCATCACATCATCATTCCCGCAATTGCCATCGCGGTTCCGGCAACGCCGGCAATCAACCCAATGGTCGGGAACAGC
GTCTTTCGGGAGATGAAGTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPKIARVLISVTDKTGIIDFASALSAMGADLISTGGTARMIREAGIPVADVSDVTGFPEMLDGRVKTMHPKIAGGILAMR
GNPEHMAAISTHGIAPIDMVVVNLYRFEEVAAKAGAHLEELIENIDIGGPTMLRAAAKNYQDVAVVVSPADYPALIEEMR
ASAGALSHETKWRLAKKVFRATADYDAAISARLEQVDAPAALLPHLAIRAAKLMDLRYGENPHQSAALYGHRGQGIAGAE
QLHGKELSYNNLVDLDAAWQLVNEFNRPAVAIIKHTNPCGCAEQDTLAAAYRKAFEADPVSAFGGVIGINRTVDEETARE
LAKTFLEAIAAPDFTAEALSILTAKKNLRLMRVAPAADPLVVKSISGGYLAQTADTAKLDRAQAVVKTRRAPTEEEWVAL
EFGWKVAKHVKSNAIVYARAGQTVGVGAGQMSRVDSVKLGAMKAVLPVAGTVVASDAFFPFPDGVEEAAKNGATAFIQPG
GSVRDEEVIAAADRLGVAMVFTGVRHFRH

Sequences:

>Translated_509_residues
MPKIARVLISVTDKTGIIDFASALSAMGADLISTGGTARMIREAGIPVADVSDVTGFPEMLDGRVKTMHPKIAGGILAMR
GNPEHMAAISTHGIAPIDMVVVNLYRFEEVAAKAGAHLEELIENIDIGGPTMLRAAAKNYQDVAVVVSPADYPALIEEMR
ASAGALSHETKWRLAKKVFRATADYDAAISARLEQVDAPAALLPHLAIRAAKLMDLRYGENPHQSAALYGHRGQGIAGAE
QLHGKELSYNNLVDLDAAWQLVNEFNRPAVAIIKHTNPCGCAEQDTLAAAYRKAFEADPVSAFGGVIGINRTVDEETARE
LAKTFLEAIAAPDFTAEALSILTAKKNLRLMRVAPAADPLVVKSISGGYLAQTADTAKLDRAQAVVKTRRAPTEEEWVAL
EFGWKVAKHVKSNAIVYARAGQTVGVGAGQMSRVDSVKLGAMKAVLPVAGTVVASDAFFPFPDGVEEAAKNGATAFIQPG
GSVRDEEVIAAADRLGVAMVFTGVRHFRH
>Mature_508_residues
PKIARVLISVTDKTGIIDFASALSAMGADLISTGGTARMIREAGIPVADVSDVTGFPEMLDGRVKTMHPKIAGGILAMRG
NPEHMAAISTHGIAPIDMVVVNLYRFEEVAAKAGAHLEELIENIDIGGPTMLRAAAKNYQDVAVVVSPADYPALIEEMRA
SAGALSHETKWRLAKKVFRATADYDAAISARLEQVDAPAALLPHLAIRAAKLMDLRYGENPHQSAALYGHRGQGIAGAEQ
LHGKELSYNNLVDLDAAWQLVNEFNRPAVAIIKHTNPCGCAEQDTLAAAYRKAFEADPVSAFGGVIGINRTVDEETAREL
AKTFLEAIAAPDFTAEALSILTAKKNLRLMRVAPAADPLVVKSISGGYLAQTADTAKLDRAQAVVKTRRAPTEEEWVALE
FGWKVAKHVKSNAIVYARAGQTVGVGAGQMSRVDSVKLGAMKAVLPVAGTVVASDAFFPFPDGVEEAAKNGATAFIQPGG
SVRDEEVIAAADRLGVAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=470, Percent_Identity=37.8723404255319, Blast_Score=263, Evalue=4e-70,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=50.0952380952381, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71985564, Length=514, Percent_Identity=35.0194552529183, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71985574, Length=342, Percent_Identity=28.0701754385965, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323056, Length=470, Percent_Identity=37.2340425531915, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6323768, Length=466, Percent_Identity=36.9098712446352, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24649832, Length=476, Percent_Identity=38.8655462184874, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54042; Mature: 53910

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIARVLISVTDKTGIIDFASALSAMGADLISTGGTARMIREAGIPVADVSDVTGFPEM
CCHHHEEHHHCCCCCCHHHHHHHHHHHCHHHHHCCCHHHHHHHCCCCCCCCHHCCCCHHH
LDGRVKTMHPKIAGGILAMRGNPEHMAAISTHGIAPIDMVVVNLYRFEEVAAKAGAHLEE
HCCCHHHCCCHHHCCEEEECCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
LIENIDIGGPTMLRAAAKNYQDVAVVVSPADYPALIEEMRASAGALSHETKWRLAKKVFR
HHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ATADYDAAISARLEQVDAPAALLPHLAIRAAKLMDLRYGENPHQSAALYGHRGQGIAGAE
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCHH
QLHGKELSYNNLVDLDAAWQLVNEFNRPAVAIIKHTNPCGCAEQDTLAAAYRKAFEADPV
HHCCCCCCCCCEECHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCH
SAFGGVIGINRTVDEETARELAKTFLEAIAAPDFTAEALSILTAKKNLRLMRVAPAADPL
HHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCE
VVKSISGGYLAQTADTAKLDRAQAVVKTRRAPTEEEWVALEFGWKVAKHVKSNAIVYARA
EEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHCCCEEEEEEC
GQTVGVGAGQMSRVDSVKLGAMKAVLPVAGTVVASDAFFPFPDGVEEAAKNGATAFIQPG
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHCCCCEEEECCC
GSVRDEEVIAAADRLGVAMVFTGVRHFRH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKIARVLISVTDKTGIIDFASALSAMGADLISTGGTARMIREAGIPVADVSDVTGFPEM
CHHHEEHHHCCCCCCHHHHHHHHHHHCHHHHHCCCHHHHHHHCCCCCCCCHHCCCCHHH
LDGRVKTMHPKIAGGILAMRGNPEHMAAISTHGIAPIDMVVVNLYRFEEVAAKAGAHLEE
HCCCHHHCCCHHHCCEEEECCCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
LIENIDIGGPTMLRAAAKNYQDVAVVVSPADYPALIEEMRASAGALSHETKWRLAKKVFR
HHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ATADYDAAISARLEQVDAPAALLPHLAIRAAKLMDLRYGENPHQSAALYGHRGQGIAGAE
HHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCHH
QLHGKELSYNNLVDLDAAWQLVNEFNRPAVAIIKHTNPCGCAEQDTLAAAYRKAFEADPV
HHCCCCCCCCCEECHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCH
SAFGGVIGINRTVDEETARELAKTFLEAIAAPDFTAEALSILTAKKNLRLMRVAPAADPL
HHHCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCE
VVKSISGGYLAQTADTAKLDRAQAVVKTRRAPTEEEWVALEFGWKVAKHVKSNAIVYARA
EEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHEEEEEHHHHHHHHHCCCEEEEEEC
GQTVGVGAGQMSRVDSVKLGAMKAVLPVAGTVVASDAFFPFPDGVEEAAKNGATAFIQPG
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHCCCCEEEECCC
GSVRDEEVIAAADRLGVAMVFTGVRHFRH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA