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The map label for this gene is cca [H]

Identifier: 116625462

GI number: 116625462

Start: 8024388

End: 8025701

Strand: Direct

Name: cca [H]

Synonym: Acid_6407

Alternate gene names: 116625462

Gene position: 8024388-8025701 (Clockwise)

Preceding gene: 116625461

Following gene: 116625466

Centisome position: 80.52

GC content: 64.16

Gene sequence:

>1314_bases
ATGAGCGATTACATCTACATGCTGGAGAGCCACCTGAGCCCGGATCAGAATCGGGCGGTGGAAGACGTGCAGGCGGCCGC
CGGCATGGTGAACGTAAACGTGTTCCTCACCGGAGGCGCGATGCGCGACATGATCGCCGGCTTCCGGATCCGCGACCTGG
ACTTCGTGGTCGAGGGCAACGCGTTGAAGATGGCGAAGGCCATCTGCGAGAAGACCGGAGCGACGCTGGTTTCGAGCGAC
GAAAACCGCAAGACCGCGGAGATCGTGTTCCCGAGCGGGGTGACGGCGCAAATCGGCATGTCTCGGCAGGAGAAATACGC
GCGCTCCGGCGGCAAGCCGCAAGTGACGCCGGCCACCATCCAGGAGGATTTGCGCGGACGGGATTTCACGGCCAACGCGA
TCGCGCTCTCCTTGAACCGCGCGTCGCGCGGACTGCTGCTCGACCCGATGAACGGCCTGGCCGATATCGAGCGCAAGGAG
CTGCGGGCGGTTTCGACGTACGGGTTCTATGACGACCCGACCCGTCTGCTGCGCCTGGTGCGGTTCAAAGTGCGCCTCGG
ATTCACCATCGAAGAGCGCACGCAGATGCAGGTGGCGAATGCTCGCGAGGCCGAAGTGGAGAAGCGGATTCCGGCGAAGG
CGCTGGGCGAGGAATTGAAGCGGATCAGCGCGGAAGATAATCCGACGGAGATTCTGAAGGGACTGGAAGAGGCGGGCCTG
CTGGCGATGTTCTCGCCGGTGCTGTTCGGCAAACTGAATCTGGCGAGCCTGGCAAAACTGGAGAAGGCGCTCCACGTGCT
GCCGGACGATGCGCGCTGGAGGGCCGCACGCTGGGGGACCTTCATGCACGCGGCGACGGAGAAGTTGTCACCGAAGGATA
AGCAGGCTCTGATCAAGGCGACGGAACTGGCGAAGAGCGACGTAGACGCGTGGCAGAAGACCGAGGCGCACGCCAAGAAG
TTGGAGGCCGCGCTGCGCTCGCCGAAGATCCGCAAACCCTCGCACGTGTACCACATCGTAGCCGCGGCGGCGCCGGAAGA
CGTGCTGTTCGTGCTCTACCACTCCGCGCTGAAGCCGGTGCAGGAGCGGCTGCGCAACCACTATCAGAAGTACCTGCCGA
TGGTCCAGGAGATCACGCCCGAGGAATGGGCGACCGTTGAGGGTAAGCCGGGCACGCCGAAGTACACGAAGGCGCGCGAG
GATTTCATCAGCGAACGCGTGAATCGCAAACCGCCGAAGCCCGTGGAAGAAGTCCCGCCGACGGAGCCTCCGGTTCCGGA
GCCCATGGGGAGCGGGCGGGGACGGGGAAGATAG

Upstream 100 bases:

>100_bases
GCGACTTCGGGCGACCGTGGAGAAGCATCACCCCCGGGCGTGATCCGGTTTTGAAGAGCTGGATGCCGGGCAGTTTAGTT
CTGTTGGAAAGCGCAATTCG

Downstream 100 bases:

>100_bases
CGGCTAAGGGCGAACCGGAAAGCGGCCCCAGTCGGGCGGCAGTCTTCCTCCGCCGCCCCATGCAATGGGTGGATGCGGCT
CTAATGGCCAACCGTCAGGT

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSDYIYMLESHLSPDQNRAVEDVQAAAGMVNVNVFLTGGAMRDMIAGFRIRDLDFVVEGNALKMAKAICEKTGATLVSSD
ENRKTAEIVFPSGVTAQIGMSRQEKYARSGGKPQVTPATIQEDLRGRDFTANAIALSLNRASRGLLLDPMNGLADIERKE
LRAVSTYGFYDDPTRLLRLVRFKVRLGFTIEERTQMQVANAREAEVEKRIPAKALGEELKRISAEDNPTEILKGLEEAGL
LAMFSPVLFGKLNLASLAKLEKALHVLPDDARWRAARWGTFMHAATEKLSPKDKQALIKATELAKSDVDAWQKTEAHAKK
LEAALRSPKIRKPSHVYHIVAAAAPEDVLFVLYHSALKPVQERLRNHYQKYLPMVQEITPEEWATVEGKPGTPKYTKARE
DFISERVNRKPPKPVEEVPPTEPPVPEPMGSGRGRGR

Sequences:

>Translated_437_residues
MSDYIYMLESHLSPDQNRAVEDVQAAAGMVNVNVFLTGGAMRDMIAGFRIRDLDFVVEGNALKMAKAICEKTGATLVSSD
ENRKTAEIVFPSGVTAQIGMSRQEKYARSGGKPQVTPATIQEDLRGRDFTANAIALSLNRASRGLLLDPMNGLADIERKE
LRAVSTYGFYDDPTRLLRLVRFKVRLGFTIEERTQMQVANAREAEVEKRIPAKALGEELKRISAEDNPTEILKGLEEAGL
LAMFSPVLFGKLNLASLAKLEKALHVLPDDARWRAARWGTFMHAATEKLSPKDKQALIKATELAKSDVDAWQKTEAHAKK
LEAALRSPKIRKPSHVYHIVAAAAPEDVLFVLYHSALKPVQERLRNHYQKYLPMVQEITPEEWATVEGKPGTPKYTKARE
DFISERVNRKPPKPVEEVPPTEPPVPEPMGSGRGRGR
>Mature_436_residues
SDYIYMLESHLSPDQNRAVEDVQAAAGMVNVNVFLTGGAMRDMIAGFRIRDLDFVVEGNALKMAKAICEKTGATLVSSDE
NRKTAEIVFPSGVTAQIGMSRQEKYARSGGKPQVTPATIQEDLRGRDFTANAIALSLNRASRGLLLDPMNGLADIERKEL
RAVSTYGFYDDPTRLLRLVRFKVRLGFTIEERTQMQVANAREAEVEKRIPAKALGEELKRISAEDNPTEILKGLEEAGLL
AMFSPVLFGKLNLASLAKLEKALHVLPDDARWRAARWGTFMHAATEKLSPKDKQALIKATELAKSDVDAWQKTEAHAKKL
EAALRSPKIRKPSHVYHIVAAAAPEDVLFVLYHSALKPVQERLRNHYQKYLPMVQEITPEEWATVEGKPGTPKYTKARED
FISERVNRKPPKPVEEVPPTEPPVPEPMGSGRGRGR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=231, Percent_Identity=30.7359307359307, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48629; Mature: 48498

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYIYMLESHLSPDQNRAVEDVQAAAGMVNVNVFLTGGAMRDMIAGFRIRDLDFVVEGN
CCCCEEHHHHCCCCCHHHHHHHHHHHCCEEEEEEEEECCHHHHHHCCCEEECEEEEEECC
ALKMAKAICEKTGATLVSSDENRKTAEIVFPSGVTAQIGMSRQEKYARSGGKPQVTPATI
HHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCHHHCCCHHHHHHHCCCCCCCCHHHH
QEDLRGRDFTANAIALSLNRASRGLLLDPMNGLADIERKELRAVSTYGFYDDPTRLLRLV
HHHHCCCCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RFKVRLGFTIEERTQMQVANAREAEVEKRIPAKALGEELKRISAEDNPTEILKGLEEAGL
HHHHHHCCEEHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCH
LAMFSPVLFGKLNLASLAKLEKALHVLPDDARWRAARWGTFMHAATEKLSPKDKQALIKA
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TELAKSDVDAWQKTEAHAKKLEAALRSPKIRKPSHVYHIVAAAAPEDVLFVLYHSALKPV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
QERLRNHYQKYLPMVQEITPEEWATVEGKPGTPKYTKAREDFISERVNRKPPKPVEEVPP
HHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCC
TEPPVPEPMGSGRGRGR
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDYIYMLESHLSPDQNRAVEDVQAAAGMVNVNVFLTGGAMRDMIAGFRIRDLDFVVEGN
CCCEEHHHHCCCCCHHHHHHHHHHHCCEEEEEEEEECCHHHHHHCCCEEECEEEEEECC
ALKMAKAICEKTGATLVSSDENRKTAEIVFPSGVTAQIGMSRQEKYARSGGKPQVTPATI
HHHHHHHHHHHHCCEEEECCCCCCEEEEEECCCCCHHHCCCHHHHHHHCCCCCCCCHHHH
QEDLRGRDFTANAIALSLNRASRGLLLDPMNGLADIERKELRAVSTYGFYDDPTRLLRLV
HHHHCCCCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RFKVRLGFTIEERTQMQVANAREAEVEKRIPAKALGEELKRISAEDNPTEILKGLEEAGL
HHHHHHCCEEHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCH
LAMFSPVLFGKLNLASLAKLEKALHVLPDDARWRAARWGTFMHAATEKLSPKDKQALIKA
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TELAKSDVDAWQKTEAHAKKLEAALRSPKIRKPSHVYHIVAAAAPEDVLFVLYHSALKPV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
QERLRNHYQKYLPMVQEITPEEWATVEGKPGTPKYTKAREDFISERVNRKPPKPVEEVPP
HHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCC
TEPPVPEPMGSGRGRGR
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA