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The map label for this gene is rfaF [H]

Identifier: 116625436

GI number: 116625436

Start: 7998032

End: 7999084

Strand: Direct

Name: rfaF [H]

Synonym: Acid_6381

Alternate gene names: 116625436

Gene position: 7998032-7999084 (Clockwise)

Preceding gene: 116625431

Following gene: 116625437

Centisome position: 80.26

GC content: 69.04

Gene sequence:

>1053_bases
GTGACCTTCAACCCCTCTTCCAGCTCGAAGGTACTTGTGCGCGCCACCAACTGGCTGGGCGACGCCGTCATGTCCCTGCC
CGCCATCCGTGCAATTCGTGAAATTTTCCCGCACGCCCACCTCGCGGTGCTGGGCCGCCCCTGGCTGGCCGACCTTTACG
CCCGCGAGAAGTCCATCGATTCCGTGATCCCCTACCCGGCGCTTACAGGACTCGGCCCGCGCCGCGATTTCGCCGCGCAA
CTCCGCGCCCGCCGCTTCGATTGTGCCATCCTGCTCCAGAACGCCTTCGACGCCGCGCTGATCACCTGGATGGCGGGCAT
TCCCGAGCGCATCGGCTACGCCCGCGATGGCCGCGGCCTCCTCCTCACCCGCCCCATCCCGGTGCCCGAGCCCGGCGAAA
TCCCGCGCCACGAGCGCTTCTATTACCTGGAGCTCCTCCGCCGCGCCGGCATGATGGAGAGCTTTCCCGCCGCCGCCGCG
ATCCGGCTCGATGGCATCGAAGACGCGCGCGAATCCGGCGCGACCCATCTTCGCTCCCTGGGAATCGAAGGCCCTGTGAT
CGGGATCAGCCCCGGCGCGGCCTATGGCAATGCCAAACGCTGGCTCCCCGAACGCTTCGCCGAGGCCGCCGCCCAGCTTC
CCGATGAAAATGCCTTCCTGATCTTCGGCTCGGCCGCCGAACGCGCTCTCTGCCAGACCGTTGCAGCGGACCTCGAGCGC
GCCGGCCGCCGCGTCCGCAACCTCGCCGGAGAAACCAGCCTGCGCGAATTCCTCGATCTCGCCGCCGCCTGCCGCCTCGT
GCTGACCAACGATTCCGGCGCCATGCACGCCGCTGGAGCGCTCGGCGTCCCCACTGTGACCATCTTCGGTGCGACCGACG
ATACCACCACCGGCCCCACCGGCCCCTTCGCCCGCATCGTCCGCGAGCACGCCGAGTGCAGCCCCTGCCTGCTCCGCGAG
TGCCCCATCGATCACCGCTGCATGACCCGCGTCACGTCCGCCCGAGTCGCCCAGGTCGCCCGCGAACTCCTCACCACTAT
CGACGCCGCTTAA

Upstream 100 bases:

>100_bases
GACTATTCTTTCATAAGAATAGACCGCCGGCAAAAAGATTGTCAGGGTATTTTCCCTTACCCTCCTCCTCGGATTTCATA
ACCCGCTATCCTGTGACTTA

Downstream 100 bases:

>100_bases
GTTACCCTGATCATTCCCGTGGACACACGAACCAAAATTGTGAGCCCGCAAGCCGCCCCCACGGGCGCCACCGTGGTCAC
CGGCACCTTCGATGTCATCC

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTFNPSSSSKVLVRATNWLGDAVMSLPAIRAIREIFPHAHLAVLGRPWLADLYAREKSIDSVIPYPALTGLGPRRDFAAQ
LRARRFDCAILLQNAFDAALITWMAGIPERIGYARDGRGLLLTRPIPVPEPGEIPRHERFYYLELLRRAGMMESFPAAAA
IRLDGIEDARESGATHLRSLGIEGPVIGISPGAAYGNAKRWLPERFAEAAAQLPDENAFLIFGSAAERALCQTVAADLER
AGRRVRNLAGETSLREFLDLAAACRLVLTNDSGAMHAAGALGVPTVTIFGATDDTTTGPTGPFARIVREHAECSPCLLRE
CPIDHRCMTRVTSARVAQVARELLTTIDAA

Sequences:

>Translated_350_residues
MTFNPSSSSKVLVRATNWLGDAVMSLPAIRAIREIFPHAHLAVLGRPWLADLYAREKSIDSVIPYPALTGLGPRRDFAAQ
LRARRFDCAILLQNAFDAALITWMAGIPERIGYARDGRGLLLTRPIPVPEPGEIPRHERFYYLELLRRAGMMESFPAAAA
IRLDGIEDARESGATHLRSLGIEGPVIGISPGAAYGNAKRWLPERFAEAAAQLPDENAFLIFGSAAERALCQTVAADLER
AGRRVRNLAGETSLREFLDLAAACRLVLTNDSGAMHAAGALGVPTVTIFGATDDTTTGPTGPFARIVREHAECSPCLLRE
CPIDHRCMTRVTSARVAQVARELLTTIDAA
>Mature_349_residues
TFNPSSSSKVLVRATNWLGDAVMSLPAIRAIREIFPHAHLAVLGRPWLADLYAREKSIDSVIPYPALTGLGPRRDFAAQL
RARRFDCAILLQNAFDAALITWMAGIPERIGYARDGRGLLLTRPIPVPEPGEIPRHERFYYLELLRRAGMMESFPAAAAI
RLDGIEDARESGATHLRSLGIEGPVIGISPGAAYGNAKRWLPERFAEAAAQLPDENAFLIFGSAAERALCQTVAADLERA
GRRVRNLAGETSLREFLDLAAACRLVLTNDSGAMHAAGALGVPTVTIFGATDDTTTGPTGPFARIVREHAECSPCLLREC
PIDHRCMTRVTSARVAQVARELLTTIDAA

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=307, Percent_Identity=34.5276872964169, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 37960; Mature: 37829

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNPSSSSKVLVRATNWLGDAVMSLPAIRAIREIFPHAHLAVLGRPWLADLYAREKSID
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
SVIPYPALTGLGPRRDFAAQLRARRFDCAILLQNAFDAALITWMAGIPERIGYARDGRGL
HCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCE
LLTRPIPVPEPGEIPRHERFYYLELLRRAGMMESFPAAAAIRLDGIEDARESGATHLRSL
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCHHEEEECCHHHHHHHHHHHHHHC
GIEGPVIGISPGAAYGNAKRWLPERFAEAAAQLPDENAFLIFGSAAERALCQTVAADLER
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
AGRRVRNLAGETSLREFLDLAAACRLVLTNDSGAMHAAGALGVPTVTIFGATDDTTTGPT
HHHHHHHHHCHHHHHHHHHHHHHHEEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCC
GPFARIVREHAECSPCLLRECPIDHRCMTRVTSARVAQVARELLTTIDAA
CHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFNPSSSSKVLVRATNWLGDAVMSLPAIRAIREIFPHAHLAVLGRPWLADLYAREKSID
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHH
SVIPYPALTGLGPRRDFAAQLRARRFDCAILLQNAFDAALITWMAGIPERIGYARDGRGL
HCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCE
LLTRPIPVPEPGEIPRHERFYYLELLRRAGMMESFPAAAAIRLDGIEDARESGATHLRSL
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHCCCCHHEEEECCHHHHHHHHHHHHHHC
GIEGPVIGISPGAAYGNAKRWLPERFAEAAAQLPDENAFLIFGSAAERALCQTVAADLER
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
AGRRVRNLAGETSLREFLDLAAACRLVLTNDSGAMHAAGALGVPTVTIFGATDDTTTGPT
HHHHHHHHHCHHHHHHHHHHHHHHEEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCC
GPFARIVREHAECSPCLLRECPIDHRCMTRVTSARVAQVARELLTTIDAA
CHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]