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The map label for this gene is thiE [C]

Identifier: 116625430

GI number: 116625430

Start: 7992321

End: 7992923

Strand: Direct

Name: thiE [C]

Synonym: Acid_6375

Alternate gene names: 116625430

Gene position: 7992321-7992923 (Clockwise)

Preceding gene: 116625429

Following gene: 116625431

Centisome position: 80.2

GC content: 65.34

Gene sequence:

>603_bases
ATGCTGCGCTACTACATTACCGACCGCCGCGCCGCGGGTGGCGTCGAACCTCTGCTGCTCCATGTCGAACGTGCGGCGCG
GGACGGTGTCGAGCGCATTCAGGTGCGCGAAAAGGACCTTTGCGCGCGTGACCTGTGCGCGCTGGTACGCCGCATCCTGG
GTCTCGCCCGGCCGCATGGCACGCAGGTCCTGGTGAACTCCCGGGTGGATGTCGCGCTCGCCGCCGGCGCGGATGGCGTG
CACCTGGCCGGAAGTTCCATTGCGCCGAAAATTCTACGCAGAATTCTGCCGTCCGGCTTCCAGATCGGTGTCTCCACGCA
CTGCTTCGCAGACCTCCGCGCGGCGGAAGCAGAAGGCGCCGATTTTGTCGTATATGGACCGGTCTTCCCGGTGGTGTCGA
AACCGGGTTACGCAGCGCACGTTGGCATCGACGGGTTGCGCCGCGCGGTGCGCGCGGTAACTATCCCGGTGATTGCGCTG
GGCGGTGTTACACAGTCGAATGCCGCGGCCTGCATTGAGGCCGGCGTCGCCGGCGTCGCCGGAATTTCGATGTTTCAAAA
CCCACTCGATCCTCCCGCGGTTGGCCAGGAACGTGCCCTATAG

Upstream 100 bases:

>100_bases
GGTCTGCGGCGAAGGCATCAACTTCCAACGCGAAGTATCGCGCGACGGCCGCATGCTTTGCCGCGCCTGCGCCGGCGAGC
GATACTACCATCCCGCTTGA

Downstream 100 bases:

>100_bases
CGGGGCATGAAGAAGATTGCATTTTTTCTAGTCTCGTATTGCGCCGCGGGACTCCCCGCGTTCGCGCAGTTCTCTCACTT
CACCGGCTTCGTTGGTGGCG

Product: thiamine-phosphate diphosphorylase

Products: diphosphate; thiamine monophosphate

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MLRYYITDRRAAGGVEPLLLHVERAARDGVERIQVREKDLCARDLCALVRRILGLARPHGTQVLVNSRVDVALAAGADGV
HLAGSSIAPKILRRILPSGFQIGVSTHCFADLRAAEAEGADFVVYGPVFPVVSKPGYAAHVGIDGLRRAVRAVTIPVIAL
GGVTQSNAAACIEAGVAGVAGISMFQNPLDPPAVGQERAL

Sequences:

>Translated_200_residues
MLRYYITDRRAAGGVEPLLLHVERAARDGVERIQVREKDLCARDLCALVRRILGLARPHGTQVLVNSRVDVALAAGADGV
HLAGSSIAPKILRRILPSGFQIGVSTHCFADLRAAEAEGADFVVYGPVFPVVSKPGYAAHVGIDGLRRAVRAVTIPVIAL
GGVTQSNAAACIEAGVAGVAGISMFQNPLDPPAVGQERAL
>Mature_200_residues
MLRYYITDRRAAGGVEPLLLHVERAARDGVERIQVREKDLCARDLCALVRRILGLARPHGTQVLVNSRVDVALAAGADGV
HLAGSSIAPKILRRILPSGFQIGVSTHCFADLRAAEAEGADFVVYGPVFPVVSKPGYAAHVGIDGLRRAVRAVTIPVIAL
GGVTQSNAAACIEAGVAGVAGISMFQNPLDPPAVGQERAL

Specific function: Condenses 4-Methyl-5-(Beta-Hydroxyethyl)-Thiazole Monophosphate (Thz-P) And 4-Amino-5-Hydroxymethyl Pyrimidine Pyrophosphate (Hmp-Pp) To Form Thiamine Monophosphate (TMP). [C]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=170, Percent_Identity=30.5882352941176, Blast_Score=73, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=134, Percent_Identity=27.6119402985075, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3

Molecular weight: Translated: 20974; Mature: 20974

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRYYITDRRAAGGVEPLLLHVERAARDGVERIQVREKDLCARDLCALVRRILGLARPHG
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TQVLVNSRVDVALAAGADGVHLAGSSIAPKILRRILPSGFQIGVSTHCFADLRAAEAEGA
CEEEEECCCCEEEECCCCCEEECCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCC
DFVVYGPVFPVVSKPGYAAHVGIDGLRRAVRAVTIPVIALGGVTQSNAAACIEAGVAGVA
CEEEECCEECCCCCCCCEEECCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHH
GISMFQNPLDPPAVGQERAL
HHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRYYITDRRAAGGVEPLLLHVERAARDGVERIQVREKDLCARDLCALVRRILGLARPHG
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TQVLVNSRVDVALAAGADGVHLAGSSIAPKILRRILPSGFQIGVSTHCFADLRAAEAEGA
CEEEEECCCCEEEECCCCCEEECCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCC
DFVVYGPVFPVVSKPGYAAHVGIDGLRRAVRAVTIPVIALGGVTQSNAAACIEAGVAGVA
CEEEECCEECCCCCCCCEEECCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHH
GISMFQNPLDPPAVGQERAL
HHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00085 {hydroxymethylpyrimidine} 0.0004 {thiazole} [C]

Substrates: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphono-oxyethyl)-thiazole

Specific reaction: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine monophosphate

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]