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The map label for this gene is mdlA [H]

Identifier: 116625408

GI number: 116625408

Start: 7971702

End: 7972850

Strand: Reverse

Name: mdlA [H]

Synonym: Acid_6353

Alternate gene names: 116625408

Gene position: 7972850-7971702 (Counterclockwise)

Preceding gene: 116625410

Following gene: 116625407

Centisome position: 80.0

GC content: 64.75

Gene sequence:

>1149_bases
ATGAATCGACGACAACTCCTGTGCGGGGCGGCGGTGCTGCCGGCGTTTTCGGCGGCGGCGGCGCAGCAGAAACAGATCAA
AATCACGGGCGTGGAGACCGACGTTCTGAAGCAGCCGCCGGGGACGCCGATCTACGACGCGATTCACCGCCTGAGCGTGG
ACAGCGGATCGGTGGTGCTCCGGCTGCGAACCGACGCCGGGATCACCGGGTGGGCGAGCGTATCCTTCGGCATGATCGCG
GGCGGCCCCCGTGTAGTGGAGACGATCCTGCAGCAGGAAGTAAAGCCGGTGCTGCTCGGCCAGGACCCGGCCTTCCCGCG
GCGCATTCGTGCCGAGTTGTGGAAGGCTCTGGAGTACAACGGCGTAGGCGGGGTGGTACAGTTCGCGATCGCCGCCGTGG
ACATCGCCGTCTGGGACATTCTCGGCAAGAGCGCCGGGCTGCCGGTCTACAAGATGCTGGGCGCGTATCGCGATCGCATG
CCGGTCTATTCGATGTGCGGCTGGTATTACGATAACGACGAGGATCTCTCGCGCTATAAGCGGTCTTTGACGCAGGCCAT
GGAGCAGGGCTATCACGCGGTAAAGATCAAAGTAGGCCGCGGGCCGGTGGAAGAAGACGTGCGCCGCATCAAGCTCGCGC
TCGACGTGGTGGGTAAGGGCAATCGCGTGATGGTGGACGCCAACCAGGTCTTCAACCGCAACGACGCGCTGCGGCGCGGG
CGCGTTTACCAGGAGATGGGCTGCTTCTGGTACGAGGAGCCGCTGCCGCCGCAGGAGATGGAGGGCTACGCGGAGCTGGC
GCGCGAGCTCGATATCCGAATCGCTACGGGCGAAAACCTGGCGACGAAATACGCCTTCGCGGACCTGATCGCGCGGCGCG
GCGCCGACGTGGTGCAGCCCGATAACCGGCGCGCCGGCGGGGTCACGGAGTGGATGGAAATCGCCGCGATTGCCGATGGC
TACGGGCTCGAGCTCGCCAGCCACGGCGGCAGCGCCACGAATTTAAATATGCTGCTCGCCATGCCCAACGCGATCTATAT
GGAGACCAGCGGCGCGCGCAAACTGGTGAACGGCGAGATGACCGCGCCTGAAGAACCGGGTATGAGCAGCGAAGTCCCTG
ACGCCGACATTCGACGCTTCAAGGTGTGA

Upstream 100 bases:

>100_bases
CTGCGCGATCAATTCGTCGGGCAGGTGGTAATCGAATTCGGAAAGGTTCACTCAGCTTTTAATGTAGCGAAAGAGGGCGA
GTGGGGGTATCGTGTAGATC

Downstream 100 bases:

>100_bases
CCGGCTTACACAAGAAATCGCGAGGAACCATGGCGAATCGTTTTGCGATCCCCGAAGGATGGCGCTTGGAGAGCAGCGGA
GAGGTCTCCACGCTGGTTGG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNRRQLLCGAAVLPAFSAAAAQQKQIKITGVETDVLKQPPGTPIYDAIHRLSVDSGSVVLRLRTDAGITGWASVSFGMIA
GGPRVVETILQQEVKPVLLGQDPAFPRRIRAELWKALEYNGVGGVVQFAIAAVDIAVWDILGKSAGLPVYKMLGAYRDRM
PVYSMCGWYYDNDEDLSRYKRSLTQAMEQGYHAVKIKVGRGPVEEDVRRIKLALDVVGKGNRVMVDANQVFNRNDALRRG
RVYQEMGCFWYEEPLPPQEMEGYAELARELDIRIATGENLATKYAFADLIARRGADVVQPDNRRAGGVTEWMEIAAIADG
YGLELASHGGSATNLNMLLAMPNAIYMETSGARKLVNGEMTAPEEPGMSSEVPDADIRRFKV

Sequences:

>Translated_382_residues
MNRRQLLCGAAVLPAFSAAAAQQKQIKITGVETDVLKQPPGTPIYDAIHRLSVDSGSVVLRLRTDAGITGWASVSFGMIA
GGPRVVETILQQEVKPVLLGQDPAFPRRIRAELWKALEYNGVGGVVQFAIAAVDIAVWDILGKSAGLPVYKMLGAYRDRM
PVYSMCGWYYDNDEDLSRYKRSLTQAMEQGYHAVKIKVGRGPVEEDVRRIKLALDVVGKGNRVMVDANQVFNRNDALRRG
RVYQEMGCFWYEEPLPPQEMEGYAELARELDIRIATGENLATKYAFADLIARRGADVVQPDNRRAGGVTEWMEIAAIADG
YGLELASHGGSATNLNMLLAMPNAIYMETSGARKLVNGEMTAPEEPGMSSEVPDADIRRFKV
>Mature_382_residues
MNRRQLLCGAAVLPAFSAAAAQQKQIKITGVETDVLKQPPGTPIYDAIHRLSVDSGSVVLRLRTDAGITGWASVSFGMIA
GGPRVVETILQQEVKPVLLGQDPAFPRRIRAELWKALEYNGVGGVVQFAIAAVDIAVWDILGKSAGLPVYKMLGAYRDRM
PVYSMCGWYYDNDEDLSRYKRSLTQAMEQGYHAVKIKVGRGPVEEDVRRIKLALDVVGKGNRVMVDANQVFNRNDALRRG
RVYQEMGCFWYEEPLPPQEMEGYAELARELDIRIATGENLATKYAFADLIARRGADVVQPDNRRAGGVTEWMEIAAIADG
YGLELASHGGSATNLNMLLAMPNAIYMETSGARKLVNGEMTAPEEPGMSSEVPDADIRRFKV

Specific function: Unknown

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=411, Percent_Identity=23.8442822384428, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI186972148, Length=336, Percent_Identity=24.702380952381, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI42544117, Length=408, Percent_Identity=22.3039215686275, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI226510960, Length=303, Percent_Identity=29.7029702970297, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI48994953, Length=356, Percent_Identity=27.247191011236, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787864, Length=372, Percent_Identity=26.3440860215054, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 42012; Mature: 42012

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRQLLCGAAVLPAFSAAAAQQKQIKITGVETDVLKQPPGTPIYDAIHRLSVDSGSVVL
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCHHHHHHHHCCCCCCEEE
RLRTDAGITGWASVSFGMIAGGPRVVETILQQEVKPVLLGQDPAFPRRIRAELWKALEYN
EEECCCCCCEEHHCCCCEECCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCC
GVGGVVQFAIAAVDIAVWDILGKSAGLPVYKMLGAYRDRMPVYSMCGWYYDNDEDLSRYK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHH
RSLTQAMEQGYHAVKIKVGRGPVEEDVRRIKLALDVVGKGNRVMVDANQVFNRNDALRRG
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHEECCCCEEEEEHHHHHCCCHHHHHH
RVYQEMGCFWYEEPLPPQEMEGYAELARELDIRIATGENLATKYAFADLIARRGADVVQP
HHHHHHCCEEECCCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCC
DNRRAGGVTEWMEIAAIADGYGLELASHGGSATNLNMLLAMPNAIYMETSGARKLVNGEM
CCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCEEEEECCCCHHCCCCCC
TAPEEPGMSSEVPDADIRRFKV
CCCCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MNRRQLLCGAAVLPAFSAAAAQQKQIKITGVETDVLKQPPGTPIYDAIHRLSVDSGSVVL
CCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCHHHHHHHHCCCCCCEEE
RLRTDAGITGWASVSFGMIAGGPRVVETILQQEVKPVLLGQDPAFPRRIRAELWKALEYN
EEECCCCCCEEHHCCCCEECCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCC
GVGGVVQFAIAAVDIAVWDILGKSAGLPVYKMLGAYRDRMPVYSMCGWYYDNDEDLSRYK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHH
RSLTQAMEQGYHAVKIKVGRGPVEEDVRRIKLALDVVGKGNRVMVDANQVFNRNDALRRG
HHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHEECCCCEEEEEHHHHHCCCHHHHHH
RVYQEMGCFWYEEPLPPQEMEGYAELARELDIRIATGENLATKYAFADLIARRGADVVQP
HHHHHHCCEEECCCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCC
DNRRAGGVTEWMEIAAIADGYGLELASHGGSATNLNMLLAMPNAIYMETSGARKLVNGEM
CCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCEEEEECCCCHHCCCCCC
TAPEEPGMSSEVPDADIRRFKV
CCCCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]