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The map label for this gene is lepA

Identifier: 116625392

GI number: 116625392

Start: 7955172

End: 7956986

Strand: Reverse

Name: lepA

Synonym: Acid_6337

Alternate gene names: 116625392

Gene position: 7956986-7955172 (Counterclockwise)

Preceding gene: 116625394

Following gene: 116625391

Centisome position: 79.84

GC content: 60.39

Gene sequence:

>1815_bases
ATGGATCGCGAATTCATCCGGAATTTCTCTATCATCGCGCACATCGATCACGGCAAGTCTACCTTGGCCGACCGTCTGCT
CGAAGTTACAGGCGCCCTCTCGCAGCGTGAAATGATGGAGCAGGTGCTTGACTCGATGGATCTCGAGCGCGAGCGCGGCA
TCACCATCAAGGCTCACGCCGTCCGCCTCAACTATCGCGCCGACGATGGCAAGCTCTACCAGCTCAACCTGATCGATACC
CCCGGGCACGTCGATTTCTCGTACGAGGTATCGCGCAGCCTGCAGGCCTGCGAAGGCGCGCTGCTCGTGGTGGACGCCTC
CCAGGGCGTGGAAGCGCAGACCCTCGCCAATACGTATCTCGCCCTCCACCATAACCTCGAGATCATTCCCGTCATTAATA
AGATCGACCTGCCCGCCGCCGAGCCCGAACGCATCCGCGAGCAGATCGAAACCGTTGTCGGCATCGACGCTCGCGATGCC
GTCCTGTGCAGCGCCAAGCAGGGCGTCGGCATCCACGATATCCTCGAAGCCATCGTCCACCTCGTGCCCCCTCCCAAGGG
CTCTCCGGATGCACCCTTGCGCGCGCTGATCTTCGATTCCTGGTTCGATAGCTACCGTGGCGTCATCATTCTGATGCGCA
TCATCGATGGCCGCATGAAGCTCGGCCAGAAGATCAAGCTGATGGCCAATGGCCAGGTCTTCGAGGTGGAAGGCCTTGGC
TACCAGGCACCCAAAGCCACTGCCTGCAGCGAATTGCAGGCTGGCGAAGTCGGCTTCCTTTACGCCAACATTAAGACCGT
GGGCGACGCCAAGATTGGCGACACCATCACGGAGGTTGAGAACCCCGCCACCGAGCCGCTCCCCGGTTTCGAAGAGATCA
AGCCCATGGTCTTCGCGGGCCTCTACCCGGTGGAGTCCCACGAGCACGGCCTGCTGCGCGATGCCTTGGAAAAGCTGCGC
CTTAACGATAGCGCCTTCAATTTCGAGCCTGAGAATTCGGCCGCGCTGGGCTTCGGTTTCCGCTGCGGCTTCCTCGGTCT
GCTCCACCTGGAAATCGTCCAGGAACGTTTGGAGCGCGAATTCAACATCGACCTGATCACCACCGCTCCCGGCGTCCGCT
ACCGCATCACCGACACCTCCGGCAATCTGGTGGAAGTCGATAACCCGACCAAGTTCCCCGACCCGTCCTACATCGAGACC
ATCGAGGAGCCGATCATCGATGCCTCCGTGATTACGCGCGAAGATTTCCTCGGCGGCATCCTCGCCCTGCTCGAAGAAAA
GCGCGGTACGCAGAAAAAGTTCGAACACATCGGCGCCGGCCGCGTCATGCTCACCTACGAGCTGCCGCTGAATGAGATCG
TGCTCGATTTCTACGATCGCCTGAAGAGCGCTTCGAAAGGTTATGCGTCGCTGGATTATCACCTCTCCGGCTATCGCGAG
TCTCCGATGGTCAAGCTCGACGTGCTCGTGGCCGGCGAACCGGTGGACGCGCTCTCCATCATCGTCCACAAGGATTTCGC
CTTTGAGCGCGGCAAAGCGCTGATCTCGCGCCTGCGCAAGCTCATCCCCAAACAGATGTTCGAGGTCGCCCTGCAGGCAG
CCATCGGCAACAAGATCGTCGCCCGCGAGACCATCTCGGCGATGCGCAAGAACGTGATCGCCAAGTGCTACGGCGGCGAT
ATCAGCCGCAAACGCAAGCTGCTCGAAAAACAGAAGGAAGGCAAAAAGCGCATGAAGCGCGTCGGCCGCGTGGAAATCCC
CCAGGAAGCCTTCCTCAGCGTCCTCAAAGTCAGCCAGTCCTCCGACGACGACTAG

Upstream 100 bases:

>100_bases
GGAAGCGCAGGTTTCGTGATGCAAGTCTCTGAAACCTGCCGCCTGCATCCGGCACCCGCCCCTGTGGGATAATTGAAATT
CAGGCCAGTCCCCCTACTAC

Downstream 100 bases:

>100_bases
TATTGCTCGCCGTACTGCACTACTGTGCATTTGTCTTCTCGCAGCCCGCGAAGCTTGGTTTGTATTACACTGCTGTTTTT
TGCTCGAACGCCTCACCGAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MDREFIRNFSIIAHIDHGKSTLADRLLEVTGALSQREMMEQVLDSMDLERERGITIKAHAVRLNYRADDGKLYQLNLIDT
PGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANTYLALHHNLEIIPVINKIDLPAAEPERIREQIETVVGIDARDA
VLCSAKQGVGIHDILEAIVHLVPPPKGSPDAPLRALIFDSWFDSYRGVIILMRIIDGRMKLGQKIKLMANGQVFEVEGLG
YQAPKATACSELQAGEVGFLYANIKTVGDAKIGDTITEVENPATEPLPGFEEIKPMVFAGLYPVESHEHGLLRDALEKLR
LNDSAFNFEPENSAALGFGFRCGFLGLLHLEIVQERLEREFNIDLITTAPGVRYRITDTSGNLVEVDNPTKFPDPSYIET
IEEPIIDASVITREDFLGGILALLEEKRGTQKKFEHIGAGRVMLTYELPLNEIVLDFYDRLKSASKGYASLDYHLSGYRE
SPMVKLDVLVAGEPVDALSIIVHKDFAFERGKALISRLRKLIPKQMFEVALQAAIGNKIVARETISAMRKNVIAKCYGGD
ISRKRKLLEKQKEGKKRMKRVGRVEIPQEAFLSVLKVSQSSDDD

Sequences:

>Translated_604_residues
MDREFIRNFSIIAHIDHGKSTLADRLLEVTGALSQREMMEQVLDSMDLERERGITIKAHAVRLNYRADDGKLYQLNLIDT
PGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANTYLALHHNLEIIPVINKIDLPAAEPERIREQIETVVGIDARDA
VLCSAKQGVGIHDILEAIVHLVPPPKGSPDAPLRALIFDSWFDSYRGVIILMRIIDGRMKLGQKIKLMANGQVFEVEGLG
YQAPKATACSELQAGEVGFLYANIKTVGDAKIGDTITEVENPATEPLPGFEEIKPMVFAGLYPVESHEHGLLRDALEKLR
LNDSAFNFEPENSAALGFGFRCGFLGLLHLEIVQERLEREFNIDLITTAPGVRYRITDTSGNLVEVDNPTKFPDPSYIET
IEEPIIDASVITREDFLGGILALLEEKRGTQKKFEHIGAGRVMLTYELPLNEIVLDFYDRLKSASKGYASLDYHLSGYRE
SPMVKLDVLVAGEPVDALSIIVHKDFAFERGKALISRLRKLIPKQMFEVALQAAIGNKIVARETISAMRKNVIAKCYGGD
ISRKRKLLEKQKEGKKRMKRVGRVEIPQEAFLSVLKVSQSSDDD
>Mature_604_residues
MDREFIRNFSIIAHIDHGKSTLADRLLEVTGALSQREMMEQVLDSMDLERERGITIKAHAVRLNYRADDGKLYQLNLIDT
PGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANTYLALHHNLEIIPVINKIDLPAAEPERIREQIETVVGIDARDA
VLCSAKQGVGIHDILEAIVHLVPPPKGSPDAPLRALIFDSWFDSYRGVIILMRIIDGRMKLGQKIKLMANGQVFEVEGLG
YQAPKATACSELQAGEVGFLYANIKTVGDAKIGDTITEVENPATEPLPGFEEIKPMVFAGLYPVESHEHGLLRDALEKLR
LNDSAFNFEPENSAALGFGFRCGFLGLLHLEIVQERLEREFNIDLITTAPGVRYRITDTSGNLVEVDNPTKFPDPSYIET
IEEPIIDASVITREDFLGGILALLEEKRGTQKKFEHIGAGRVMLTYELPLNEIVLDFYDRLKSASKGYASLDYHLSGYRE
SPMVKLDVLVAGEPVDALSIIVHKDFAFERGKALISRLRKLIPKQMFEVALQAAIGNKIVARETISAMRKNVIAKCYGGD
ISRKRKLLEKQKEGKKRMKRVGRVEIPQEAFLSVLKVSQSSDDD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=605, Percent_Identity=51.2396694214876, Blast_Score=630, Evalue=1e-180,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=47.7272727272727, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=39.375, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI25306283, Length=152, Percent_Identity=45.3947368421053, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=45.3947368421053, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=45.3947368421053, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=41.6107382550336, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=44.954128440367, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=44.954128440367, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=44.954128440367, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI217272894, Length=138, Percent_Identity=37.6811594202899, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI217272892, Length=138, Percent_Identity=37.6811594202899, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI53729339, Length=245, Percent_Identity=27.3469387755102, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=245, Percent_Identity=27.3469387755102, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI94966752, Length=85, Percent_Identity=45.8823529411765, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=59.3959731543624, Blast_Score=718, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=30.7086614173228, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1789738, Length=207, Percent_Identity=31.8840579710145, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=222, Percent_Identity=26.5765765765766, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=42.3895253682488, Blast_Score=495, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17556745, Length=204, Percent_Identity=37.2549019607843, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17533571, Length=145, Percent_Identity=38.6206896551724, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=38.5093167701863, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=39.5522388059701, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=39.5522388059701, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=36.0902255639098, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=45.4242928452579, Blast_Score=528, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=37.7049180327869, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=43.3566433566434, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=43.3566433566434, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=41.0714285714286, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=174, Percent_Identity=37.9310344827586, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=243, Percent_Identity=24.6913580246914, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=45.0331125827815, Blast_Score=558, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24582462, Length=185, Percent_Identity=36.7567567567568, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.8848920863309, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=39.4557823129252, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=39.4557823129252, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=39.4557823129252, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221458488, Length=153, Percent_Identity=40.5228758169935, Blast_Score=97, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357743, Length=140, Percent_Identity=38.5714285714286, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_SOLUE (Q01SV7)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827548.1
- ProteinModelPortal:   Q01SV7
- SMR:   Q01SV7
- STRING:   Q01SV7
- GeneID:   4430590
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_6337
- NMPDR:   fig|234267.9.peg.6020
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- PhylomeDB:   Q01SV7
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67244; Mature: 67244

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDREFIRNFSIIAHIDHGKSTLADRLLEVTGALSQREMMEQVLDSMDLERERGITIKAHA
CCHHHHHCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCCEEEEEE
VRLNYRADDGKLYQLNLIDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANTYL
EEEEEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
ALHHNLEIIPVINKIDLPAAEPERIREQIETVVGIDARDAVLCSAKQGVGIHDILEAIVH
HEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHEEEECCCCCCHHHHHHHHHH
LVPPPKGSPDAPLRALIFDSWFDSYRGVIILMRIIDGRMKLGQKIKLMANGQVFEVEGLG
HCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCC
YQAPKATACSELQAGEVGFLYANIKTVGDAKIGDTITEVENPATEPLPGFEEIKPMVFAG
CCCCCCHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHCHHHHCC
LYPVESHEHGLLRDALEKLRLNDSAFNFEPENSAALGFGFRCGFLGLLHLEIVQERLERE
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
FNIDLITTAPGVRYRITDTSGNLVEVDNPTKFPDPSYIETIEEPIIDASVITREDFLGGI
CCEEEEECCCCEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
LALLEEKRGTQKKFEHIGAGRVMLTYELPLNEIVLDFYDRLKSASKGYASLDYHLSGYRE
HHHHHHHCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
SPMVKLDVLVAGEPVDALSIIVHKDFAFERGKALISRLRKLIPKQMFEVALQAAIGNKIV
CCCEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARETISAMRKNVIAKCYGGDISRKRKLLEKQKEGKKRMKRVGRVEIPQEAFLSVLKVSQS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
SDDD
CCCC
>Mature Secondary Structure
MDREFIRNFSIIAHIDHGKSTLADRLLEVTGALSQREMMEQVLDSMDLERERGITIKAHA
CCHHHHHCCEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHCCCEEEEEE
VRLNYRADDGKLYQLNLIDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANTYL
EEEEEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
ALHHNLEIIPVINKIDLPAAEPERIREQIETVVGIDARDAVLCSAKQGVGIHDILEAIVH
HEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHEEEECCCCCCHHHHHHHHHH
LVPPPKGSPDAPLRALIFDSWFDSYRGVIILMRIIDGRMKLGQKIKLMANGQVFEVEGLG
HCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCC
YQAPKATACSELQAGEVGFLYANIKTVGDAKIGDTITEVENPATEPLPGFEEIKPMVFAG
CCCCCCHHHHHCCCCCEEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHCHHHHCC
LYPVESHEHGLLRDALEKLRLNDSAFNFEPENSAALGFGFRCGFLGLLHLEIVQERLERE
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
FNIDLITTAPGVRYRITDTSGNLVEVDNPTKFPDPSYIETIEEPIIDASVITREDFLGGI
CCEEEEECCCCEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
LALLEEKRGTQKKFEHIGAGRVMLTYELPLNEIVLDFYDRLKSASKGYASLDYHLSGYRE
HHHHHHHCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
SPMVKLDVLVAGEPVDALSIIVHKDFAFERGKALISRLRKLIPKQMFEVALQAAIGNKIV
CCCEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARETISAMRKNVIAKCYGGDISRKRKLLEKQKEGKKRMKRVGRVEIPQEAFLSVLKVSQS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
SDDD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA