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The map label for this gene is oprM [H]

Identifier: 116625388

GI number: 116625388

Start: 7950151

End: 7951563

Strand: Reverse

Name: oprM [H]

Synonym: Acid_6333

Alternate gene names: 116625388

Gene position: 7951563-7950151 (Counterclockwise)

Preceding gene: 116625389

Following gene: 116625387

Centisome position: 79.79

GC content: 62.85

Gene sequence:

>1413_bases
GTGCAATGTACCCACCGGCGCGCGGTAAGCAGCACCCCGCTCTTCGTGTGCCTCGCGCTGCTGTTCTCGTCCGTACTGGC
CGCACAGGAGAACAGCCCGTTCCAGGGCAGCGTGCCAACCGGTAACGCAACCACGACTCCCCTCGGATTGAAACTGGACG
ATGCCATCCAGCGGGGGCTTCGCGCCAACCTGGGTCTGCTCGAGCGCGGGACAGCCAGTCAGGCCGCGCGCGCGGAACGC
ATTCGAGCCCTCTCCGCGCTGTACCCGCAGGTGACGGGTAGCGTCGGGGAAACCGTACAGCAACTGAACTTGAAAACCCT
GGGCTTCGGGACCTTGGGCATTCCAGGACTCAGCGGCCTTCCCTCAATCGCGGGCCCATTCTCCTACAGCACCGCACAAG
CCAACGTCTCCGCGACGGTCTTCGATTGGAGCAAGCGGAAAAATCTCAGCTCCGCCCGCGACACTGAAGAAGCCTCGAAG
CTCTCCATCCAGGATGCGCGCGACCTGGTGGTGCAGGCGGTTGCGTACGCGTACTTGCAGATCATCGCGGACAACTCCCG
CGTGGAATCGATCCAGGCTCAGGTCACCACAGCCGAAGCCATATACAACCGCGCTGTGGATCAGAAAAAAGCCGGCGTCG
CCCCGGCGATCGATGTGCTGCGCGCCCAGGTGGAGTTCAAAACCCAGCAGCAGCGGCTCCTGGTGGAACAGAACCAGTTC
GCGAAAGACAAACTCACCCTGGGGCGCGTGATCGGGCTGCCTCCCGGCCAACCCTTCTACATTGCCGATGCCACTCCGTT
CACCCCGTTCAGCGGACTGACTCTGGATCAGGCCTTGCGTACCGCCGTCGCCCAAAGAGCCGATTACCAGAGCGCGCGCA
AACTGTTTCTGGCGGCCGATGATGCCCTGAAAGCCGCGCGAGCCGAATGGTACCCGACCGTCGATCTGAACGGATACTAC
GGTGTTACCGGCCCGACCCCTGGCAACTCGCATGGAGTTTTTCTGGTAACGGGTGCCCTCAACTTCAATATCTTTAATGG
CGGGCGGATTCACGGCGACATCGAGCAGGCACGGGCCGCGAAGCAACAGCGCGCCGACGAGCTTGCCGACCTGGGAGGCC
AGATCGAGGTGCAGGTGCGGACCGCATTCCTCGACATCCAATCGGCGGCCGACCAGGTGGCAGTGGCGCAAAGTAACGTG
GAACTGGCCGCTCAGACGCTCCAACAGGCCAGGGATCGCTTCGCCTCGGGTGTCGCCGACACGATCGAGGTGGTGCAGGC
GCAGGAATCGGTGGCCACCGCCAGCGACAACCTGATTTCCGCGTCGTACGCCCACAATCTTGCCAAGGCATCGCTGGCGC
GCGCCTTGGGCCTGGCCGAGCAGGGGATCAAAAAGTTGATTGAGGTGAAATGA

Upstream 100 bases:

>100_bases
AGCGAAGAAGCTCCTGAAAATGGATGATTCTCAACCCGGTGACCGCCGGGCTCTGCCGCATTTTGAGATCGGAATGGGGC
CTGCCCGCATCGGGTGTCAC

Downstream 100 bases:

>100_bases
AATGGCCGACACGTTGGTCGAGACGCCAAAGACGGAAACTCCACCGCCGCGCATCGTCAGAAAGCCAAAGCGCCACCGTG
GCGCGTTGATCGCAATGTTG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MQCTHRRAVSSTPLFVCLALLFSSVLAAQENSPFQGSVPTGNATTTPLGLKLDDAIQRGLRANLGLLERGTASQAARAER
IRALSALYPQVTGSVGETVQQLNLKTLGFGTLGIPGLSGLPSIAGPFSYSTAQANVSATVFDWSKRKNLSSARDTEEASK
LSIQDARDLVVQAVAYAYLQIIADNSRVESIQAQVTTAEAIYNRAVDQKKAGVAPAIDVLRAQVEFKTQQQRLLVEQNQF
AKDKLTLGRVIGLPPGQPFYIADATPFTPFSGLTLDQALRTAVAQRADYQSARKLFLAADDALKAARAEWYPTVDLNGYY
GVTGPTPGNSHGVFLVTGALNFNIFNGGRIHGDIEQARAAKQQRADELADLGGQIEVQVRTAFLDIQSAADQVAVAQSNV
ELAAQTLQQARDRFASGVADTIEVVQAQESVATASDNLISASYAHNLAKASLARALGLAEQGIKKLIEVK

Sequences:

>Translated_470_residues
MQCTHRRAVSSTPLFVCLALLFSSVLAAQENSPFQGSVPTGNATTTPLGLKLDDAIQRGLRANLGLLERGTASQAARAER
IRALSALYPQVTGSVGETVQQLNLKTLGFGTLGIPGLSGLPSIAGPFSYSTAQANVSATVFDWSKRKNLSSARDTEEASK
LSIQDARDLVVQAVAYAYLQIIADNSRVESIQAQVTTAEAIYNRAVDQKKAGVAPAIDVLRAQVEFKTQQQRLLVEQNQF
AKDKLTLGRVIGLPPGQPFYIADATPFTPFSGLTLDQALRTAVAQRADYQSARKLFLAADDALKAARAEWYPTVDLNGYY
GVTGPTPGNSHGVFLVTGALNFNIFNGGRIHGDIEQARAAKQQRADELADLGGQIEVQVRTAFLDIQSAADQVAVAQSNV
ELAAQTLQQARDRFASGVADTIEVVQAQESVATASDNLISASYAHNLAKASLARALGLAEQGIKKLIEVK
>Mature_470_residues
MQCTHRRAVSSTPLFVCLALLFSSVLAAQENSPFQGSVPTGNATTTPLGLKLDDAIQRGLRANLGLLERGTASQAARAER
IRALSALYPQVTGSVGETVQQLNLKTLGFGTLGIPGLSGLPSIAGPFSYSTAQANVSATVFDWSKRKNLSSARDTEEASK
LSIQDARDLVVQAVAYAYLQIIADNSRVESIQAQVTTAEAIYNRAVDQKKAGVAPAIDVLRAQVEFKTQQQRLLVEQNQF
AKDKLTLGRVIGLPPGQPFYIADATPFTPFSGLTLDQALRTAVAQRADYQSARKLFLAADDALKAARAEWYPTVDLNGYY
GVTGPTPGNSHGVFLVTGALNFNIFNGGRIHGDIEQARAAKQQRADELADLGGQIEVQVRTAFLDIQSAADQVAVAQSNV
ELAAQTLQQARDRFASGVADTIEVVQAQESVATASDNLISASYAHNLAKASLARALGLAEQGIKKLIEVK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50097; Mature: 50097

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCTHRRAVSSTPLFVCLALLFSSVLAAQENSPFQGSVPTGNATTTPLGLKLDDAIQRGL
CCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RANLGLLERGTASQAARAERIRALSALYPQVTGSVGETVQQLNLKTLGFGTLGIPGLSGL
HCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCCCCCCCCCC
PSIAGPFSYSTAQANVSATVFDWSKRKNLSSARDTEEASKLSIQDARDLVVQAVAYAYLQ
CCCCCCCCCCCCCCCCEEEEEECHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IIADNSRVESIQAQVTTAEAIYNRAVDQKKAGVAPAIDVLRAQVEFKTQQQRLLVEQNQF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AKDKLTLGRVIGLPPGQPFYIADATPFTPFSGLTLDQALRTAVAQRADYQSARKLFLAAD
HHHHHHHHHEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECC
DALKAARAEWYPTVDLNGYYGVTGPTPGNSHGVFLVTGALNFNIFNGGRIHGDIEQARAA
HHHHHHHHCCCCEEECCCEECCCCCCCCCCCCEEEEEEEEEEEEECCCEECCCHHHHHHH
KQQRADELADLGGQIEVQVRTAFLDIQSAADQVAVAQSNVELAAQTLQQARDRFASGVAD
HHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
TIEVVQAQESVATASDNLISASYAHNLAKASLARALGLAEQGIKKLIEVK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQCTHRRAVSSTPLFVCLALLFSSVLAAQENSPFQGSVPTGNATTTPLGLKLDDAIQRGL
CCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RANLGLLERGTASQAARAERIRALSALYPQVTGSVGETVQQLNLKTLGFGTLGIPGLSGL
HCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECCCCCCCCCCCCC
PSIAGPFSYSTAQANVSATVFDWSKRKNLSSARDTEEASKLSIQDARDLVVQAVAYAYLQ
CCCCCCCCCCCCCCCCEEEEEECHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IIADNSRVESIQAQVTTAEAIYNRAVDQKKAGVAPAIDVLRAQVEFKTQQQRLLVEQNQF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AKDKLTLGRVIGLPPGQPFYIADATPFTPFSGLTLDQALRTAVAQRADYQSARKLFLAAD
HHHHHHHHHEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECC
DALKAARAEWYPTVDLNGYYGVTGPTPGNSHGVFLVTGALNFNIFNGGRIHGDIEQARAA
HHHHHHHHCCCCEEECCCEECCCCCCCCCCCCEEEEEEEEEEEEECCCEECCCHHHHHHH
KQQRADELADLGGQIEVQVRTAFLDIQSAADQVAVAQSNVELAAQTLQQARDRFASGVAD
HHHHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
TIEVVQAQESVATASDNLISASYAHNLAKASLARALGLAEQGIKKLIEVK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]