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The map label for this gene is emrB [H]

Identifier: 116625386

GI number: 116625386

Start: 7947255

End: 7948865

Strand: Reverse

Name: emrB [H]

Synonym: Acid_6331

Alternate gene names: 116625386

Gene position: 7948865-7947255 (Counterclockwise)

Preceding gene: 116625387

Following gene: 116625385

Centisome position: 79.76

GC content: 60.52

Gene sequence:

>1611_bases
ATGGCCGAAGTAACCGAGACGCTCCCGCCGCCGGCACGCGCACCACAACGCTGGGAACCGAAGGCCAATCCGTGGCTAAT
TGCCGTGGTGGTTGCGTTGGCGGCCTTCATGGAAGTGCTGGACACGAGCATTGCCAATGTCGCCCTGCCGCACATAGCAG
GCAACCTCGGCGCCAGCAACGATCAGAGCACCTGGGTTCTCACGTCCTACCTGGTTTCGAACGCGATTGTTCTTCCCATC
AGTGGTTGGCTGGCGAGCCTGTTCGGCCGAAGGCGTTTCTTCCTGCTGTGCATCGTCCTCTTCACGGTGAGTTCGGTGTT
ATGCGGATCCGCTCCCAGCCTGGGCCTGTTGATCGTATTCCGCGCCATACAGGGGGTCGGCGGCGGTGGATTGCAACCCA
TAGCCCAGGCCATTCTTGCCGACACCTTTCCACCGCAGAAACGCGGCCTGGCCTTCGCGCTATACGGAATCACTGCCGTG
ATGGCGCCTACCATCGGTCCGACCCTGGGCGGGTGGATCACCGACAACTATTCCTGGCGATGGATCTTCTACATCAACCT
TCCGATTGGGATGATGACGCTGTTCCTGGTGCACCGCCTGGTAGAAGATCCACCGTATCTCCGGCGCCTGAGCGGCGCCG
GTATCCGCGTGGACTATATCGGCATCGGGTTGTTGGCGCTCGGAATCGGCGCCCTGCAGGTGGTACTCGACAAGGGCCAG
GAGGACGATTGGTTCGGTTCTCGCTTCATCCTTACGCTCTCCATCGTTTCGGCGGCTTGCCTGATCTCTCTGGTGATCTG
GGAATGGTTCCACCGGGACCCCGTGATCGACGTCCATCTCTTCAAGAACTTCAACTTCGCCGCGGCGAACCTGATGATGT
TCATGCTTGGAGTAGTGTACTTCAGCAGCCTGGTGATGATGCCGCAGTTCCTGCAGACACTGTTGGGCTATACGGCGGAG
TTGGCCGGACTGGTGCTTTCGGCGAGCGGAATCCTGCTGCTCGTGTGCATGCCCATTGTCGGCCAGCTGACCACGAAATT
CCCAGCGAAGTACATCATTGCGTTCGGCTGGCTCAGCATCGCAGCGGCGCTATTTTATTCCACCAAACGAATCGATCTGG
AGATCAGCTTCCGCGTCGCGATGTGGCTCCGGGTGGCGCAGGCGGCCGGCCTGCCGTTTCTGTTTGTGCCGATCACGCTG
GCGAGTTACACCGGGCTCCCGGCCGCCAAGAGCAACGACGCATCGGGTCTGATCAATTTCATGCGCAACATCGGCGCCAG
CGTGGGAACGTCGCTGGTGACCACAATGCTGGCGCGGCGGGCGCAATTCCACCAGTCGGTGATGTCCTATCACACCACCA
ACTACGACACCGTGTTTCAGAATCAGGTGGCCGCCGCGACCCAGCAGCTCATCCACGCCGGTGCTAGCGCGGCGGATGCG
CGACTCCAGGCCTACGGGATGGTGTACCAATCGATGCAAGCCCAGGCGCAGACTCTGGCCTATATAGATACCTACAAAAT
GCTTGCCATCGGCGCGAGCATCATGTTCGTGCTCGCCTTTGTGGTCCGGAAGAACGACCCCCGGGCGGGCGGAGAGGTTG
CCGCGGGTTAA

Upstream 100 bases:

>100_bases
TCGCATCGATCTCGACCCCGGTCAGAACGCCGATCATCACCTGCGTCCGGGAATGTCCGTTGATCCGAAGGTTTTCCTGC
GATAGCGAATCGTTGGGTAT

Downstream 100 bases:

>100_bases
AGAATCCATGATCAGAGTGATTACGATCGAGCGCGAATACGGCAGCGGAGGCGCCGACATCGCGAAGCAACTGGCGCACC
GGCTGGGCTGGCAGTTGTGG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MAEVTETLPPPARAPQRWEPKANPWLIAVVVALAAFMEVLDTSIANVALPHIAGNLGASNDQSTWVLTSYLVSNAIVLPI
SGWLASLFGRRRFFLLCIVLFTVSSVLCGSAPSLGLLIVFRAIQGVGGGGLQPIAQAILADTFPPQKRGLAFALYGITAV
MAPTIGPTLGGWITDNYSWRWIFYINLPIGMMTLFLVHRLVEDPPYLRRLSGAGIRVDYIGIGLLALGIGALQVVLDKGQ
EDDWFGSRFILTLSIVSAACLISLVIWEWFHRDPVIDVHLFKNFNFAAANLMMFMLGVVYFSSLVMMPQFLQTLLGYTAE
LAGLVLSASGILLLVCMPIVGQLTTKFPAKYIIAFGWLSIAAALFYSTKRIDLEISFRVAMWLRVAQAAGLPFLFVPITL
ASYTGLPAAKSNDASGLINFMRNIGASVGTSLVTTMLARRAQFHQSVMSYHTTNYDTVFQNQVAAATQQLIHAGASAADA
RLQAYGMVYQSMQAQAQTLAYIDTYKMLAIGASIMFVLAFVVRKNDPRAGGEVAAG

Sequences:

>Translated_536_residues
MAEVTETLPPPARAPQRWEPKANPWLIAVVVALAAFMEVLDTSIANVALPHIAGNLGASNDQSTWVLTSYLVSNAIVLPI
SGWLASLFGRRRFFLLCIVLFTVSSVLCGSAPSLGLLIVFRAIQGVGGGGLQPIAQAILADTFPPQKRGLAFALYGITAV
MAPTIGPTLGGWITDNYSWRWIFYINLPIGMMTLFLVHRLVEDPPYLRRLSGAGIRVDYIGIGLLALGIGALQVVLDKGQ
EDDWFGSRFILTLSIVSAACLISLVIWEWFHRDPVIDVHLFKNFNFAAANLMMFMLGVVYFSSLVMMPQFLQTLLGYTAE
LAGLVLSASGILLLVCMPIVGQLTTKFPAKYIIAFGWLSIAAALFYSTKRIDLEISFRVAMWLRVAQAAGLPFLFVPITL
ASYTGLPAAKSNDASGLINFMRNIGASVGTSLVTTMLARRAQFHQSVMSYHTTNYDTVFQNQVAAATQQLIHAGASAADA
RLQAYGMVYQSMQAQAQTLAYIDTYKMLAIGASIMFVLAFVVRKNDPRAGGEVAAG
>Mature_535_residues
AEVTETLPPPARAPQRWEPKANPWLIAVVVALAAFMEVLDTSIANVALPHIAGNLGASNDQSTWVLTSYLVSNAIVLPIS
GWLASLFGRRRFFLLCIVLFTVSSVLCGSAPSLGLLIVFRAIQGVGGGGLQPIAQAILADTFPPQKRGLAFALYGITAVM
APTIGPTLGGWITDNYSWRWIFYINLPIGMMTLFLVHRLVEDPPYLRRLSGAGIRVDYIGIGLLALGIGALQVVLDKGQE
DDWFGSRFILTLSIVSAACLISLVIWEWFHRDPVIDVHLFKNFNFAAANLMMFMLGVVYFSSLVMMPQFLQTLLGYTAEL
AGLVLSASGILLLVCMPIVGQLTTKFPAKYIIAFGWLSIAAALFYSTKRIDLEISFRVAMWLRVAQAAGLPFLFVPITLA
SYTGLPAAKSNDASGLINFMRNIGASVGTSLVTTMLARRAQFHQSVMSYHTTNYDTVFQNQVAAATQQLIHAGASAADAR
LQAYGMVYQSMQAQAQTLAYIDTYKMLAIGASIMFVLAFVVRKNDPRAGGEVAAG

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=484, Percent_Identity=36.3636363636364, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=35.6713426853707, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI1790195, Length=337, Percent_Identity=31.1572700296736, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87081983, Length=433, Percent_Identity=28.6374133949192, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1788392, Length=443, Percent_Identity=28.2167042889391, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790146, Length=158, Percent_Identity=31.0126582278481, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789312, Length=178, Percent_Identity=28.0898876404494, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=481, Percent_Identity=24.948024948025, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=34.6666666666667, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=140, Percent_Identity=32.8571428571429, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323735, Length=301, Percent_Identity=23.2558139534884, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319783, Length=392, Percent_Identity=23.9795918367347, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324264, Length=208, Percent_Identity=24.5192307692308, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=301, Percent_Identity=26.578073089701, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58123; Mature: 57992

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00092 N6_MTASE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVTETLPPPARAPQRWEPKANPWLIAVVVALAAFMEVLDTSIANVALPHIAGNLGASN
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DQSTWVLTSYLVSNAIVLPISGWLASLFGRRRFFLLCIVLFTVSSVLCGSAPSLGLLIVF
CCCHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RAIQGVGGGGLQPIAQAILADTFPPQKRGLAFALYGITAVMAPTIGPTLGGWITDNYSWR
HHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEECCCCEE
WIFYINLPIGMMTLFLVHRLVEDPPYLRRLSGAGIRVDYIGIGLLALGIGALQVVLDKGQ
EEEEEECCHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
EDDWFGSRFILTLSIVSAACLISLVIWEWFHRDPVIDVHLFKNFNFAAANLMMFMLGVVY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
FSSLVMMPQFLQTLLGYTAELAGLVLSASGILLLVCMPIVGQLTTKFPAKYIIAFGWLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AAALFYSTKRIDLEISFRVAMWLRVAQAAGLPFLFVPITLASYTGLPAAKSNDASGLINF
HHHHHHHHHEEEEEEHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHH
MRNIGASVGTSLVTTMLARRAQFHQSVMSYHTTNYDTVFQNQVAAATQQLIHAGASAADA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
RLQAYGMVYQSMQAQAQTLAYIDTYKMLAIGASIMFVLAFVVRKNDPRAGGEVAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AEVTETLPPPARAPQRWEPKANPWLIAVVVALAAFMEVLDTSIANVALPHIAGNLGASN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DQSTWVLTSYLVSNAIVLPISGWLASLFGRRRFFLLCIVLFTVSSVLCGSAPSLGLLIVF
CCCHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
RAIQGVGGGGLQPIAQAILADTFPPQKRGLAFALYGITAVMAPTIGPTLGGWITDNYSWR
HHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEECCCCEE
WIFYINLPIGMMTLFLVHRLVEDPPYLRRLSGAGIRVDYIGIGLLALGIGALQVVLDKGQ
EEEEEECCHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
EDDWFGSRFILTLSIVSAACLISLVIWEWFHRDPVIDVHLFKNFNFAAANLMMFMLGVVY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
FSSLVMMPQFLQTLLGYTAELAGLVLSASGILLLVCMPIVGQLTTKFPAKYIIAFGWLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AAALFYSTKRIDLEISFRVAMWLRVAQAAGLPFLFVPITLASYTGLPAAKSNDASGLINF
HHHHHHHHHEEEEEEHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHH
MRNIGASVGTSLVTTMLARRAQFHQSVMSYHTTNYDTVFQNQVAAATQQLIHAGASAADA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
RLQAYGMVYQSMQAQAQTLAYIDTYKMLAIGASIMFVLAFVVRKNDPRAGGEVAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]