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The map label for this gene is grpE [H]

Identifier: 116625374

GI number: 116625374

Start: 7937674

End: 7938330

Strand: Direct

Name: grpE [H]

Synonym: Acid_6319

Alternate gene names: 116625374

Gene position: 7937674-7938330 (Clockwise)

Preceding gene: 116625373

Following gene: 116625375

Centisome position: 79.65

GC content: 64.99

Gene sequence:

>657_bases
ATGGATCGCGCCGAGATTCTGCGACGCTTCGAACTGTGGCTTGATACCGCCATGGAGGACGAAGCTCCGCCGCCCGGCAT
TCCGCCGGAGATTCTGTCGGGAGATGCCGGCACGGAAACCGCCGCTACCGACTCGTACGCAATGTGGGCGGCGATGACCG
CGCTCACTCAGGAAGTGAAACTCCAGGGCCGCGCTTTCAAACAATTGAGCGAGACCCTTTCCGCCGAAGGCGAACGGCGC
GGCCGCAAGGAATCGCTGGGCGCGCTGCTCGAAATGCGCGAACGGCTGGCGCGCGGATTGGAAGGATTGCGTGGTCTGGA
GGAAGTGCGGCCCGGCTTTTGGGACCGTATCTTCGCTGCGCGGTGGCGGCAGGTGGAGCATGCGCTCGGCGTGGTACGCG
CCATGGAGGAAGGCTACCGGCTGACCTTGGACCGGCTGGACGATCTGCTTTCCGAATTTCAGGTGCACCCGATCGAGTGC
GAGGGCCTGCAGTTCGACCCGCGCCGCATGAACGCCGTCGATGTCGAAGAGACCGACCGCGCGGCCGAGGGCACCGTGCT
GACCGTCTACCGCGCCGGTTACGAATGGAATGGCGAGTTATACCGTCCCGCGCAGGTGCGGGTGGCGAAGCGTCCGGTGA
ACGGAGGAGTGCGATGA

Upstream 100 bases:

>100_bases
CGCACCAGGCGCTTACCACGCTGCTCGATGGGCGCGCGGCCGAACGGCGGTTCGTAGGCCCCGAACCTTGGCTGGCGGTG
CTGAAGGAGTCCCGGCGCTG

Downstream 100 bases:

>100_bases
GCGATCTGGTGGTAGGAATTGACCTGGGCACGACGAACTCGGAAGTCGCCGCGTTCCTAAATGGCCGCGTGCGCGTGCTG
GGCCCGAATGCGAGCAAAAT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MDRAEILRRFELWLDTAMEDEAPPPGIPPEILSGDAGTETAATDSYAMWAAMTALTQEVKLQGRAFKQLSETLSAEGERR
GRKESLGALLEMRERLARGLEGLRGLEEVRPGFWDRIFAARWRQVEHALGVVRAMEEGYRLTLDRLDDLLSEFQVHPIEC
EGLQFDPRRMNAVDVEETDRAAEGTVLTVYRAGYEWNGELYRPAQVRVAKRPVNGGVR

Sequences:

>Translated_218_residues
MDRAEILRRFELWLDTAMEDEAPPPGIPPEILSGDAGTETAATDSYAMWAAMTALTQEVKLQGRAFKQLSETLSAEGERR
GRKESLGALLEMRERLARGLEGLRGLEEVRPGFWDRIFAARWRQVEHALGVVRAMEEGYRLTLDRLDDLLSEFQVHPIEC
EGLQFDPRRMNAVDVEETDRAAEGTVLTVYRAGYEWNGELYRPAQVRVAKRPVNGGVR
>Mature_218_residues
MDRAEILRRFELWLDTAMEDEAPPPGIPPEILSGDAGTETAATDSYAMWAAMTALTQEVKLQGRAFKQLSETLSAEGERR
GRKESLGALLEMRERLARGLEGLRGLEEVRPGFWDRIFAARWRQVEHALGVVRAMEEGYRLTLDRLDDLLSEFQVHPIEC
EGLQFDPRRMNAVDVEETDRAAEGTVLTVYRAGYEWNGELYRPAQVRVAKRPVNGGVR

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

None

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24689; Mature: 24689

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRAEILRRFELWLDTAMEDEAPPPGIPPEILSGDAGTETAATDSYAMWAAMTALTQEVK
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LQGRAFKQLSETLSAEGERRGRKESLGALLEMRERLARGLEGLRGLEEVRPGFWDRIFAA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RWRQVEHALGVVRAMEEGYRLTLDRLDDLLSEFQVHPIECEGLQFDPRRMNAVDVEETDR
HHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCC
AAEGTVLTVYRAGYEWNGELYRPAQVRVAKRPVNGGVR
CCCCCEEEEEECCEECCCCEECHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDRAEILRRFELWLDTAMEDEAPPPGIPPEILSGDAGTETAATDSYAMWAAMTALTQEVK
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LQGRAFKQLSETLSAEGERRGRKESLGALLEMRERLARGLEGLRGLEEVRPGFWDRIFAA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RWRQVEHALGVVRAMEEGYRLTLDRLDDLLSEFQVHPIECEGLQFDPRRMNAVDVEETDR
HHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCC
AAEGTVLTVYRAGYEWNGELYRPAQVRVAKRPVNGGVR
CCCCCEEEEEECCEECCCCEECHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA