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The map label for this gene is prpB [C]

Identifier: 116625252

GI number: 116625252

Start: 7776401

End: 7777213

Strand: Reverse

Name: prpB [C]

Synonym: Acid_6197

Alternate gene names: 116625252

Gene position: 7777213-7776401 (Counterclockwise)

Preceding gene: 116625253

Following gene: 116625251

Centisome position: 78.04

GC content: 66.54

Gene sequence:

>813_bases
ATGACGGCGGCATTCAGAAAGCTGCACGAGAGCGGCTGCTTCGTGATCCCGAACCCGTGGGATATCGGCAGCGCGCGATA
CCTGGCGCGCCTGGGCTTTCGCGCGCTGGCGACTACCAGCTCCGGGTTCGCCTTTTCGCGCGGGCTGCCGGATTCGCCCA
AGGCGGTTTCGCGCGAGATGATGCTCGCTCACGTCGCTGAGATGGTCGGGGCCGCGGGATTGCCGGTGAATGCGGATTTC
CAGAATGGCTACGCGGACGAACCGGAGGACGTGGCGGAGAGCGTGCTCCGCTGCGTGGCGACAGGGGTGGCGGGGCTTTC
GATCGAAGATGCCACCGGCAACGCGAACGCTCCATTGTACGAACTGCGCGCAGCCACCGAGCGGATCGCGGCGGCGCGGA
GCGCGATCGACCGCTCCGGCGCCGGAGTCCTGTTGACGGCCCGGGCGGAGTGCTATCTGGTTGGACAGGAGGACCCGCTG
CGGGAATCGATCCGCCGTTTGACGGCCTACGCCGCCGCAGGGGCGGACGTGTTGTACGCGCCCGGGGTGCGCGACCGCGA
GGAGATCGCGGCGATGGTCAGGGCGGTGCATCCGAAACCGGTCAACGTGCTGATGTTTGCGGACACCGGGCTGCGCGTGA
GCGACCTGGCGGAGTTGGGAGTGCGTCGCGTCAGCGTCGGATCGTCGCTGGCGCGCAGCGCCTGGACGGGCTTCATCCGG
GCGGCAAAGGCGATTGCGGAAGAAGGCAGTTTCGCCGGATTTGAGGGGAATGTTCCGTTTGGAGACCTCGATGGCTTTTT
TAGAAATTGGTAG

Upstream 100 bases:

>100_bases
GCCAGTTCGAAGGGGAGCCGCAGGGAGGCCGCGGGCAGCGGCTGCGCGCGATTCTCGATGACAAGGAGTTCGTGAAGGAA
GTGTTGCAGTGATCGCTTCA

Downstream 100 bases:

>100_bases
CCACTTTGGGCTAGTGGCACTATGAAAAACGGGAACAAGGCGCCGCTCTGTGTAAGCGCTTGTAAAGTCCACGGGAATTT
GGGCATTCAATCTCCCGAAC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTAAFRKLHESGCFVIPNPWDIGSARYLARLGFRALATTSSGFAFSRGLPDSPKAVSREMMLAHVAEMVGAAGLPVNADF
QNGYADEPEDVAESVLRCVATGVAGLSIEDATGNANAPLYELRAATERIAAARSAIDRSGAGVLLTARAECYLVGQEDPL
RESIRRLTAYAAAGADVLYAPGVRDREEIAAMVRAVHPKPVNVLMFADTGLRVSDLAELGVRRVSVGSSLARSAWTGFIR
AAKAIAEEGSFAGFEGNVPFGDLDGFFRNW

Sequences:

>Translated_270_residues
MTAAFRKLHESGCFVIPNPWDIGSARYLARLGFRALATTSSGFAFSRGLPDSPKAVSREMMLAHVAEMVGAAGLPVNADF
QNGYADEPEDVAESVLRCVATGVAGLSIEDATGNANAPLYELRAATERIAAARSAIDRSGAGVLLTARAECYLVGQEDPL
RESIRRLTAYAAAGADVLYAPGVRDREEIAAMVRAVHPKPVNVLMFADTGLRVSDLAELGVRRVSVGSSLARSAWTGFIR
AAKAIAEEGSFAGFEGNVPFGDLDGFFRNW
>Mature_269_residues
TAAFRKLHESGCFVIPNPWDIGSARYLARLGFRALATTSSGFAFSRGLPDSPKAVSREMMLAHVAEMVGAAGLPVNADFQ
NGYADEPEDVAESVLRCVATGVAGLSIEDATGNANAPLYELRAATERIAAARSAIDRSGAGVLLTARAECYLVGQEDPLR
ESIRRLTAYAAAGADVLYAPGVRDREEIAAMVRAVHPKPVNVLMFADTGLRVSDLAELGVRRVSVGSSLARSAWTGFIRA
AKAIAEEGSFAGFEGNVPFGDLDGFFRNW

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28656; Mature: 28525

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAFRKLHESGCFVIPNPWDIGSARYLARLGFRALATTSSGFAFSRGLPDSPKAVSREM
CCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHH
MLAHVAEMVGAAGLPVNADFQNGYADEPEDVAESVLRCVATGVAGLSIEDATGNANAPLY
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHH
ELRAATERIAAARSAIDRSGAGVLLTARAECYLVGQEDPLRESIRRLTAYAAAGADVLYA
HHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEC
PGVRDREEIAAMVRAVHPKPVNVLMFADTGLRVSDLAELGVRRVSVGSSLARSAWTGFIR
CCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKAIAEEGSFAGFEGNVPFGDLDGFFRNW
HHHHHHHCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TAAFRKLHESGCFVIPNPWDIGSARYLARLGFRALATTSSGFAFSRGLPDSPKAVSREM
CHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHH
MLAHVAEMVGAAGLPVNADFQNGYADEPEDVAESVLRCVATGVAGLSIEDATGNANAPLY
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHH
ELRAATERIAAARSAIDRSGAGVLLTARAECYLVGQEDPLRESIRRLTAYAAAGADVLYA
HHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEC
PGVRDREEIAAMVRAVHPKPVNVLMFADTGLRVSDLAELGVRRVSVGSSLARSAWTGFIR
CCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKAIAEEGSFAGFEGNVPFGDLDGFFRNW
HHHHHHHCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]