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The map label for this gene is insF1 [C]

Identifier: 116625132

GI number: 116625132

Start: 7596785

End: 7597627

Strand: Reverse

Name: insF1 [C]

Synonym: Acid_6069

Alternate gene names: 116625132

Gene position: 7597627-7596785 (Counterclockwise)

Preceding gene: 116625133

Following gene: 116625131

Centisome position: 76.24

GC content: 57.77

Gene sequence:

>843_bases
ATGAGTGTCGTGCAGATGTGCGAGTCGCTGGGCATCAGCCGGGCAGGATACTACCGCTTTTTGGATCCGGAAAAGCCGGC
ACCCGCCGATATGGATCTTCGCGACGAGATGCACCGGGTAGCCCTCGATTGGCCCTGCTATGGGAGCCGGCGTATCGTCG
AGGAACTCAAGGCTCGTGGATGGGAGGTCAACCGCAAGCGGATTCAGCGGCTGATGCGGGAAGACAACCTGCTGTGTGTG
ATCAAACGCAAGTTCGTCGTAGCCACCACCGATTCCCGTCACGGTCTGAAGGTATATCCGAATCGCGCCGCGGAGATGAC
GCTAACCGGAGTCGATCAGCTCTGGGTGGCCGATATCACCTACATCCGACTGGAAGAGGAGTTTGTCTACCTGGCGGTCA
TTCTGGAGGCCTTCTCACGCCGGGTCATTGGCTGGCATTTGGGGGAAACCTTAGAAGTTTCCCTCACGCTGAATGCCTTG
CGCATGGCGCTTGGGCAACGATCGGTAAGTCCGGGCCCGGTTCACCACTCCGACCGCGGAGTCCAGTATGCCAGTCACGA
TTACACGCAACTGCTCCAGGACAACGGGATCGAAATCAGCATGAGTCGCAAGGCCAACCCCTGGGACAATGCGGCCTGCG
AGTCCTTCATGAAAACGCTGAAATACGAGGAGGTACACCGCACCGAATATCGGAATCTGGCACACGCCCGTGCGTCGATA
AAGACCTTCCTGGAGAAGATCTATAACCAAAAGCGATTGCACTCGTCCTTGAGCTATCGCTCGCCGGTCGAGTTCGAACA
CTCTCTAGCGGCCACCGCTTCTCAGCAGCAGGTGGCGGTATGA

Upstream 100 bases:

>100_bases
GGGCCTTGCAGCATGTCGAGGAACAGCGCCTGCTGCGGGCACTGAACAACGGCGCGCCCTCTACGAGCAGATCGACAAAG
AAGTCCAGGCGGAAACCCGC

Downstream 100 bases:

>100_bases
AAAGTGAGGGCGCCCTCAGGAAGCGTTTTCTCAGGCATGACGGAATCTATCGGTCCGATATGTCATTCCATCTGATAAAC
CCGGTCCAGCTGCCGCCTCC

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARGWEVNRKRIQRLMREDNLLCV
IKRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADITYIRLEEEFVYLAVILEAFSRRVIGWHLGETLEVSLTLNAL
RMALGQRSVSPGPVHHSDRGVQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASI
KTFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV

Sequences:

>Translated_280_residues
MSVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARGWEVNRKRIQRLMREDNLLCV
IKRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADITYIRLEEEFVYLAVILEAFSRRVIGWHLGETLEVSLTLNAL
RMALGQRSVSPGPVHHSDRGVQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASI
KTFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV
>Mature_279_residues
SVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARGWEVNRKRIQRLMREDNLLCVI
KRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADITYIRLEEEFVYLAVILEAFSRRVIGWHLGETLEVSLTLNALR
MALGQRSVSPGPVHHSDRGVQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASIK
TFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=239, Percent_Identity=32.6359832635983, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1788405, Length=273, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1787263, Length=273, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786753, Length=273, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786570, Length=273, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786490, Length=273, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1789981, Length=222, Percent_Identity=31.981981981982, Blast_Score=107, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32288; Mature: 32157

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARG
CCHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHEECCCCCCCHHHHHHHHHCC
WEVNRKRIQRLMREDNLLCVIKRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADIT
CCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCEEECCCCCCEEEECCCHHHHHHHEE
YIRLEEEFVYLAVILEAFSRRVIGWHLGETLEVSLTLNALRMALGQRSVSPGPVHHSDRG
EEEEHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCC
VQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASI
CCCCCHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVVQMCESLGISRAGYYRFLDPEKPAPADMDLRDEMHRVALDWPCYGSRRIVEELKARG
CHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHEECCCCCCCHHHHHHHHHCC
WEVNRKRIQRLMREDNLLCVIKRKFVVATTDSRHGLKVYPNRAAEMTLTGVDQLWVADIT
CCCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCEEECCCCCCEEEECCCHHHHHHHEE
YIRLEEEFVYLAVILEAFSRRVIGWHLGETLEVSLTLNALRMALGQRSVSPGPVHHSDRG
EEEEHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCC
VQYASHDYTQLLQDNGIEISMSRKANPWDNAACESFMKTLKYEEVHRTEYRNLAHARASI
CCCCCHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTFLEKIYNQKRLHSSLSYRSPVEFEHSLAATASQQQVAV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]