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The map label for this gene is nfo

Identifier: 116625054

GI number: 116625054

Start: 7507956

End: 7508828

Strand: Reverse

Name: nfo

Synonym: Acid_5984

Alternate gene names: 116625054

Gene position: 7508828-7507956 (Counterclockwise)

Preceding gene: 116625055

Following gene: 116625052

Centisome position: 75.35

GC content: 65.64

Gene sequence:

>873_bases
TTGCGCCTCGGCATCCACACTTTCACGTCCGGATCGCTCGAAACCGCGGCGCTCACTGCCGGCGATTTGGGGGCCGCCAC
CTTCCAGATCTTCTCCGCGAGTCCTCGAATGTGGCGCGCCCGCGTTCCCGACCCCGTGCAGATCAAGCTTCTCTGCGCCG
CTCGCGAGCGCTTCAGCCTCTCCCCGCTGGTCATCCATACGAACTACCTGGTGAACCTAGCCTCGCTCGACCCGGTAATC
CGGGCGAAATCGATCGATGCCTTCCGCGGCGAATTGCAGCGCGCCATCGCGATCGGCGCCGAATACCTGGTGGTCCATCC
CGGCAGTTATCGCGGCCACACCGTAGAGGAAGGCATCGCAGCCTTCGCACTCGGCCTGCGCGATGCCTCGGCCGGTCTGC
CGCCCCACCAACTTACGGTGTTGCTGGAAAACACCGCCGGCGCGGGCTGCCATCTCGGGGGTAAGTTCGAGGAACTCCGC
TCGATCCGCGACCTCACCGCCGAGCTGACCGATCTCCCCATCGGCTACTGCCTGGATACCTGTCACCTGCTGGCCGCGGG
CTTCGATATCGTCACGGCACCCGGACTGCGCGCCACCCTTCGCGCCGCCGAAGCCGTGCTCGGCCTGGCCAACGTCCACG
TCTTTCACGCCAATGACAGCAGGTTCCCGCGCGGCTCCCACGTGGATCGCCATGCCCACATCGGCGAAGGCTTCATCGGC
GCCGACGCCTTCCGCCGCATCCTCACCCACCCCAAACTGCGGCGCAAGCCCTTCATTCTCGAGACCCCGGTAGACGAGCC
CGGCGACGACCGGCGCAATCTCGACATACTCAAGTCGCTCGCCGGTTCTAAGTTGCCTTTACCGGCCCGATAA

Upstream 100 bases:

>100_bases
CATCCTCGATGCCCTCAACTCGCACTACCGCATCGACCGCCAAAGTTCCCGCTCGGTGTTCTATGTGCCTGTCGAATGAC
GCCTCTGTTAGCATGAAGGC

Downstream 100 bases:

>100_bases
CATTGGCTATCTTGCCCGGTCCCATCCCGATCAGTCGCGAAACCGTCGTATCCTGCTGCTGGTCTTCCTCGTCGCCGTCT
TCCAGATTCATGTGTGCGTA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRLGIHTFTSGSLETAALTAGDLGAATFQIFSASPRMWRARVPDPVQIKLLCAARERFSLSPLVIHTNYLVNLASLDPVI
RAKSIDAFRGELQRAIAIGAEYLVVHPGSYRGHTVEEGIAAFALGLRDASAGLPPHQLTVLLENTAGAGCHLGGKFEELR
SIRDLTAELTDLPIGYCLDTCHLLAAGFDIVTAPGLRATLRAAEAVLGLANVHVFHANDSRFPRGSHVDRHAHIGEGFIG
ADAFRRILTHPKLRRKPFILETPVDEPGDDRRNLDILKSLAGSKLPLPAR

Sequences:

>Translated_290_residues
MRLGIHTFTSGSLETAALTAGDLGAATFQIFSASPRMWRARVPDPVQIKLLCAARERFSLSPLVIHTNYLVNLASLDPVI
RAKSIDAFRGELQRAIAIGAEYLVVHPGSYRGHTVEEGIAAFALGLRDASAGLPPHQLTVLLENTAGAGCHLGGKFEELR
SIRDLTAELTDLPIGYCLDTCHLLAAGFDIVTAPGLRATLRAAEAVLGLANVHVFHANDSRFPRGSHVDRHAHIGEGFIG
ADAFRRILTHPKLRRKPFILETPVDEPGDDRRNLDILKSLAGSKLPLPAR
>Mature_290_residues
MRLGIHTFTSGSLETAALTAGDLGAATFQIFSASPRMWRARVPDPVQIKLLCAARERFSLSPLVIHTNYLVNLASLDPVI
RAKSIDAFRGELQRAIAIGAEYLVVHPGSYRGHTVEEGIAAFALGLRDASAGLPPHQLTVLLENTAGAGCHLGGKFEELR
SIRDLTAELTDLPIGYCLDTCHLLAAGFDIVTAPGLRATLRAAEAVLGLANVHVFHANDSRFPRGSHVDRHAHIGEGFIG
ADAFRRILTHPKLRRKPFILETPVDEPGDDRRNLDILKSLAGSKLPLPAR

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=268, Percent_Identity=36.9402985074627, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531193, Length=263, Percent_Identity=34.2205323193916, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6322735, Length=292, Percent_Identity=29.1095890410959, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_SOLUE (Q01TU5)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827210.1
- ProteinModelPortal:   Q01TU5
- SMR:   Q01TU5
- STRING:   Q01TU5
- GeneID:   4425799
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5984
- NMPDR:   fig|234267.9.peg.5695
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   Q01TU5
- ProtClustDB:   CLSK774521
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31331; Mature: 31331

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGIHTFTSGSLETAALTAGDLGAATFQIFSASPRMWRARVPDPVQIKLLCAARERFSL
CCEEEEEECCCCCCEEEEECCCCCHHEEEEEECCCCEEECCCCCCEEEEEEEEHHHCCCC
SPLVIHTNYLVNLASLDPVIRAKSIDAFRGELQRAIAIGAEYLVVHPGSYRGHTVEEGIA
CEEEEECHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHH
AFALGLRDASAGLPPHQLTVLLENTAGAGCHLGGKFEELRSIRDLTAELTDLPIGYCLDT
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
CHLLAAGFDIVTAPGLRATLRAAEAVLGLANVHVFHANDSRFPRGSHVDRHAHIGEGFIG
HHHHHHCCCEEECCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHCCCCCCCC
ADAFRRILTHPKLRRKPFILETPVDEPGDDRRNLDILKSLAGSKLPLPAR
HHHHHHHHCCCCHHCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRLGIHTFTSGSLETAALTAGDLGAATFQIFSASPRMWRARVPDPVQIKLLCAARERFSL
CCEEEEEECCCCCCEEEEECCCCCHHEEEEEECCCCEEECCCCCCEEEEEEEEHHHCCCC
SPLVIHTNYLVNLASLDPVIRAKSIDAFRGELQRAIAIGAEYLVVHPGSYRGHTVEEGIA
CEEEEECHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHH
AFALGLRDASAGLPPHQLTVLLENTAGAGCHLGGKFEELRSIRDLTAELTDLPIGYCLDT
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
CHLLAAGFDIVTAPGLRATLRAAEAVLGLANVHVFHANDSRFPRGSHVDRHAHIGEGFIG
HHHHHHCCCEEECCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHCCCCCCCC
ADAFRRILTHPKLRRKPFILETPVDEPGDDRRNLDILKSLAGSKLPLPAR
HHHHHHHHCCCCHHCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA