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The map label for this gene is 116625037

Identifier: 116625037

GI number: 116625037

Start: 7484734

End: 7485690

Strand: Direct

Name: 116625037

Synonym: Acid_5967

Alternate gene names: NA

Gene position: 7484734-7485690 (Clockwise)

Preceding gene: 116625033

Following gene: 116625039

Centisome position: 75.11

GC content: 68.44

Gene sequence:

>957_bases
ATGGACCAATCGAACCTTCTGACCGTGGGGCTGGCGCAGATGGCGCCGTGCTGGATGGACCGCGCGCGGACGCTGGAGAA
AGTCGCGGCCTGGGTGGAGCGCGCGGCGGACGCCGGCTGCGGCATCGTGGCCTTCGGCGAGGCGCTGGTCCCGGGCTATC
CCTTCTGGGTGGAGCTGACCGACGGGGCGCGCTTCAATTCGCCGCGGCAGAAGGAGATCTTCGCCGAGTATGCGGAGCAG
GCGGTGCAGCCGGAAGCCGGCCATTTGGACGCGGTCTGCGCGGCGGCGGCGCGGCGCAAGATCGCGGTCTACCTCGGATG
CATCGAGCGCGCGGTTTCGCGCGGCGGCCACAGCCTCTACTGTTCGCTCGTCTTCATCGACGCGAACGGGAAGATCGGAA
GCGTGCACCGCAAGATGATGCCGACCTACGAAGAGCGCCTGGTGTGGAGCACAGGCGACGGGCACGGCCTGCAGACGCAC
GCGGTGGGCGCGTTCCATGCGGGCGGGCTGAACTGCTGGGAAAACTGGATGCCGCTTTCGCGGACGGCGCTGTACGCGCA
AGGCGAGGACCTGCATATCGCGGTCTGGCCGGGCAGCACCCGAAACACGCGCGACATCACGCGTTTCATCGCGCAGGAAT
CGCGCTCGTACGTGGCCAGCGTGTCCGGGCTGATGCGGCGGACCGATATCGGCGGCGATAGCGAGTGGCGGCGTGCGATG
TCCGAAGCCGCGCCGGAGGTGATGGCCGATGGCGGCAGCTGCCTGGCGGGTCCCGATGGCGAGTGGGTGATCGCTCCCGC
CGGAAATGAGGAGACGCTGTTGACAGCGACGATCGATTTCCGCCGCGTGCTGGAGGAGCGGCACAACTTCGATGCGGCAG
GCCACTACGCGCGGCCGGACGTGACGCGGTTGACGGTGGACCGGAGGAGACAGGGCATCGCGGAGTTCGTGGAGTAG

Upstream 100 bases:

>100_bases
GTTGAAAGCGTTCTGGCAAAGCTCGTGAAGTTGGGCGGGTTGGGTGGCGGGCAACGGGCCTCCTTGGAAAAAGCGAGTTT
TGATTATACCTTCAAGAACT

Downstream 100 bases:

>100_bases
TCCTGTGCTACTTCACTTCGATGACGATCAGATCGGCGGGGGTGGTGCCAATGTTGGAGGTGCGGTGCTTGATGTAGCCG
TCGGTCCAGAAGGCATCGCC

Product: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MDQSNLLTVGLAQMAPCWMDRARTLEKVAAWVERAADAGCGIVAFGEALVPGYPFWVELTDGARFNSPRQKEIFAEYAEQ
AVQPEAGHLDAVCAAAARRKIAVYLGCIERAVSRGGHSLYCSLVFIDANGKIGSVHRKMMPTYEERLVWSTGDGHGLQTH
AVGAFHAGGLNCWENWMPLSRTALYAQGEDLHIAVWPGSTRNTRDITRFIAQESRSYVASVSGLMRRTDIGGDSEWRRAM
SEAAPEVMADGGSCLAGPDGEWVIAPAGNEETLLTATIDFRRVLEERHNFDAAGHYARPDVTRLTVDRRRQGIAEFVE

Sequences:

>Translated_318_residues
MDQSNLLTVGLAQMAPCWMDRARTLEKVAAWVERAADAGCGIVAFGEALVPGYPFWVELTDGARFNSPRQKEIFAEYAEQ
AVQPEAGHLDAVCAAAARRKIAVYLGCIERAVSRGGHSLYCSLVFIDANGKIGSVHRKMMPTYEERLVWSTGDGHGLQTH
AVGAFHAGGLNCWENWMPLSRTALYAQGEDLHIAVWPGSTRNTRDITRFIAQESRSYVASVSGLMRRTDIGGDSEWRRAM
SEAAPEVMADGGSCLAGPDGEWVIAPAGNEETLLTATIDFRRVLEERHNFDAAGHYARPDVTRLTVDRRRQGIAEFVE
>Mature_318_residues
MDQSNLLTVGLAQMAPCWMDRARTLEKVAAWVERAADAGCGIVAFGEALVPGYPFWVELTDGARFNSPRQKEIFAEYAEQ
AVQPEAGHLDAVCAAAARRKIAVYLGCIERAVSRGGHSLYCSLVFIDANGKIGSVHRKMMPTYEERLVWSTGDGHGLQTH
AVGAFHAGGLNCWENWMPLSRTALYAQGEDLHIAVWPGSTRNTRDITRFIAQESRSYVASVSGLMRRTDIGGDSEWRRAM
SEAAPEVMADGGSCLAGPDGEWVIAPAGNEETLLTATIDFRRVLEERHNFDAAGHYARPDVTRLTVDRRRQGIAEFVE

Specific function: Acts on aliphatic nitriles such as acrylonitrile, crotononitrile and glutaronitrile [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=309, Percent_Identity=33.0097087378641, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322027, Length=207, Percent_Identity=34.2995169082126, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 34929; Mature: 34929

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00920 NITRIL_CHT_1 ; PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQSNLLTVGLAQMAPCWMDRARTLEKVAAWVERAADAGCGIVAFGEALVPGYPFWVELT
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCCCEEEEEE
DGARFNSPRQKEIFAEYAEQAVQPEAGHLDAVCAAAARRKIAVYLGCIERAVSRGGHSLY
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
CSLVFIDANGKIGSVHRKMMPTYEERLVWSTGDGHGLQTHAVGAFHAGGLNCWENWMPLS
EEEEEEECCCCCHHHHHHHCCCHHHCEEEECCCCCCCEECCCCEECCCCCHHHHHCCCCH
RTALYAQGEDLHIAVWPGSTRNTRDITRFIAQESRSYVASVSGLMRRTDIGGDSEWRRAM
HHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SEAAPEVMADGGSCLAGPDGEWVIAPAGNEETLLTATIDFRRVLEERHNFDAAGHYARPD
HHHCHHHHCCCCCEECCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCC
VTRLTVDRRRQGIAEFVE
HHEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MDQSNLLTVGLAQMAPCWMDRARTLEKVAAWVERAADAGCGIVAFGEALVPGYPFWVELT
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCCCEEEEEE
DGARFNSPRQKEIFAEYAEQAVQPEAGHLDAVCAAAARRKIAVYLGCIERAVSRGGHSLY
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
CSLVFIDANGKIGSVHRKMMPTYEERLVWSTGDGHGLQTHAVGAFHAGGLNCWENWMPLS
EEEEEEECCCCCHHHHHHHCCCHHHCEEEECCCCCCCEECCCCEECCCCCHHHHHCCCCH
RTALYAQGEDLHIAVWPGSTRNTRDITRFIAQESRSYVASVSGLMRRTDIGGDSEWRRAM
HHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SEAAPEVMADGGSCLAGPDGEWVIAPAGNEETLLTATIDFRRVLEERHNFDAAGHYARPD
HHHCHHHHCCCCCEECCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCC
VTRLTVDRRRQGIAEFVE
HHEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1390687 [H]