Click here to switch to the map view.

The map label for this gene is lhgO [H]

Identifier: 116624928

GI number: 116624928

Start: 7331019

End: 7332215

Strand: Direct

Name: lhgO [H]

Synonym: Acid_5855

Alternate gene names: 116624928

Gene position: 7331019-7332215 (Clockwise)

Preceding gene: 116624921

Following gene: 116624930

Centisome position: 73.56

GC content: 65.25

Gene sequence:

>1197_bases
ATGAACCTGCAGCGAGTCGCGATCATCGGCGGCGGAATTGTCGGCCTTGCCACGGCGTACCGCCTGAGCGAGCGCTTCCC
CGGCGCGCGGATCTGGGTACTGGAAAAAGAGCCAGGCGTCGGAAGGCACCAGACCGGACATAATAGCGGCGTCCTGCACT
GTGGACTCTACTACAAACCCGGAACGGTAAAAGCACGGCTGGCCGTCACGGGCATTCGCCAGATGGTGGAATTCTGCCGC
GAGAATGCCGTGCCGCACGAAATCTGCGGCAAGCTGGTAGTGGCCGCGGACGATAGCGAAGTGCCGCGACTACGAGCCCT
CGAGGAACGCGGCTGCGCCAACGGGCTCGACGGGCTGCGCTGGATGAATCGCGGCGAGATGCGCGAGATCGAGCCGCACG
TCGGCGGCGTGGCGGCGCTGCGGGTTCCCCAGGAAGGCATCGTAGATTACCCGCGCGTCTGTGAGCGCCTGGTGGCGCGG
CTTACCGAGCGCGGCGTCAAAGTAGTGACCGGAGCGCGCGTGCAGCGGTTGGACCGGCAGGGCGAGGGCTGGATCGCGCG
GACCACGGCGGGGATCTTCGAGTGCGACTTCATCATCAATTGCGCCGGTCTGCATTCCGACCGCGTGGCGGAGATCGCGG
GCGAGCGCCGCGAGATGCGCATCCTGCCTTTTCGCGGTGAGTATTACCAGATCCGCAAGGAACGGCAGCACCTGGTACGT
CACCTGATCTATCCGGTTCCGGACCCGAGCTTTCCGTTCCTGGGAGTGCACTTCACCCGGCTGATTCACGGCGGCATCGA
GGCCGGGCCCAACGCGGTGCTGGCGTTCGCGCGCGAGGGATATCGCAAGACCGACTTCAACCCGGGTGACCTCTTCGATG
CGCTCACTTACGGCGGGTTTTGGCGCTTCCTTCGACGCTATCCCTCGATGGCCTGGTACGAGCTGCGGCGGTCGTTCAGC
CGCCAGCTTTTCTGCGCGTCGCTGCAACGGCTGGTGCCGGATATACGGCCCGACGATCTGGAGACGGGTGGCAGCGGGGT
GCGTGCGCAGGCGATCGCGCCCGAAGGCGAGATCGTACAGGATTTCCGCTTAATCGCGCGCTCCAATGCGCTGCACGTCC
TCAATGCGCCCAGTCCGGCAGCGACGGCGTCACTGGCGATCGGGGAGGAAATCGTGGAGATGGTGGCGAAAGGATAG

Upstream 100 bases:

>100_bases
TTGGTCTTACGGCCTTCGCTCTTCTCACCTTGTTGTACAGAGTTGGATGGTAGTCGCGGATAGGTGATCGCGGAAGCCGC
GGGCTACACTCAAAGGGAAC

Downstream 100 bases:

>100_bases
ACTCGAACTCGTCACTTGGCCGCGGGGACCCGGCGCCGGAGATCGTCCATGAAGTTGAGCAGGAACACTGCGTGCGTCGT
CTCTTTCTGGCCGGCGGACG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNLQRVAIIGGGIVGLATAYRLSERFPGARIWVLEKEPGVGRHQTGHNSGVLHCGLYYKPGTVKARLAVTGIRQMVEFCR
ENAVPHEICGKLVVAADDSEVPRLRALEERGCANGLDGLRWMNRGEMREIEPHVGGVAALRVPQEGIVDYPRVCERLVAR
LTERGVKVVTGARVQRLDRQGEGWIARTTAGIFECDFIINCAGLHSDRVAEIAGERREMRILPFRGEYYQIRKERQHLVR
HLIYPVPDPSFPFLGVHFTRLIHGGIEAGPNAVLAFAREGYRKTDFNPGDLFDALTYGGFWRFLRRYPSMAWYELRRSFS
RQLFCASLQRLVPDIRPDDLETGGSGVRAQAIAPEGEIVQDFRLIARSNALHVLNAPSPAATASLAIGEEIVEMVAKG

Sequences:

>Translated_398_residues
MNLQRVAIIGGGIVGLATAYRLSERFPGARIWVLEKEPGVGRHQTGHNSGVLHCGLYYKPGTVKARLAVTGIRQMVEFCR
ENAVPHEICGKLVVAADDSEVPRLRALEERGCANGLDGLRWMNRGEMREIEPHVGGVAALRVPQEGIVDYPRVCERLVAR
LTERGVKVVTGARVQRLDRQGEGWIARTTAGIFECDFIINCAGLHSDRVAEIAGERREMRILPFRGEYYQIRKERQHLVR
HLIYPVPDPSFPFLGVHFTRLIHGGIEAGPNAVLAFAREGYRKTDFNPGDLFDALTYGGFWRFLRRYPSMAWYELRRSFS
RQLFCASLQRLVPDIRPDDLETGGSGVRAQAIAPEGEIVQDFRLIARSNALHVLNAPSPAATASLAIGEEIVEMVAKG
>Mature_398_residues
MNLQRVAIIGGGIVGLATAYRLSERFPGARIWVLEKEPGVGRHQTGHNSGVLHCGLYYKPGTVKARLAVTGIRQMVEFCR
ENAVPHEICGKLVVAADDSEVPRLRALEERGCANGLDGLRWMNRGEMREIEPHVGGVAALRVPQEGIVDYPRVCERLVAR
LTERGVKVVTGARVQRLDRQGEGWIARTTAGIFECDFIINCAGLHSDRVAEIAGERREMRILPFRGEYYQIRKERQHLVR
HLIYPVPDPSFPFLGVHFTRLIHGGIEAGPNAVLAFAREGYRKTDFNPGDLFDALTYGGFWRFLRRYPSMAWYELRRSFS
RQLFCASLQRLVPDIRPDDLETGGSGVRAQAIAPEGEIVQDFRLIARSNALHVLNAPSPAATASLAIGEEIVEMVAKG

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=399, Percent_Identity=40.6015037593985, Blast_Score=302, Evalue=3e-82,
Organism=Escherichia coli, GI87082148, Length=381, Percent_Identity=41.7322834645669, Blast_Score=324, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17565764, Length=398, Percent_Identity=42.4623115577889, Blast_Score=319, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24585081, Length=392, Percent_Identity=42.6020408163265, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 44344; Mature: 44344

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQRVAIIGGGIVGLATAYRLSERFPGARIWVLEKEPGVGRHQTGHNSGVLHCGLYYKP
CCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECC
GTVKARLAVTGIRQMVEFCRENAVPHEICGKLVVAADDSEVPRLRALEERGCANGLDGLR
CCCEEHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCCCHHHHHHHCCCCCCCHHHH
WMNRGEMREIEPHVGGVAALRVPQEGIVDYPRVCERLVARLTERGVKVVTGARVQRLDRQ
HCCCCCCCCCCCCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCC
GEGWIARTTAGIFECDFIINCAGLHSDRVAEIAGERREMRILPFRGEYYQIRKERQHLVR
CCCEEEEECCCEEEEEEEEEECCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHH
HLIYPVPDPSFPFLGVHFTRLIHGGIEAGPNAVLAFAREGYRKTDFNPGDLFDALTYGGF
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
WRFLRRYPSMAWYELRRSFSRQLFCASLQRLVPDIRPDDLETGGSGVRAQAIAPEGEIVQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHH
DFRLIARSNALHVLNAPSPAATASLAIGEEIVEMVAKG
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLQRVAIIGGGIVGLATAYRLSERFPGARIWVLEKEPGVGRHQTGHNSGVLHCGLYYKP
CCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECC
GTVKARLAVTGIRQMVEFCRENAVPHEICGKLVVAADDSEVPRLRALEERGCANGLDGLR
CCCEEHHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCCCHHHHHHHCCCCCCCHHHH
WMNRGEMREIEPHVGGVAALRVPQEGIVDYPRVCERLVARLTERGVKVVTGARVQRLDRQ
HCCCCCCCCCCCCCCCEEEEECCHHCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCC
GEGWIARTTAGIFECDFIINCAGLHSDRVAEIAGERREMRILPFRGEYYQIRKERQHLVR
CCCEEEEECCCEEEEEEEEEECCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHH
HLIYPVPDPSFPFLGVHFTRLIHGGIEAGPNAVLAFAREGYRKTDFNPGDLFDALTYGGF
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
WRFLRRYPSMAWYELRRSFSRQLFCASLQRLVPDIRPDDLETGGSGVRAQAIAPEGEIVQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHH
DFRLIARSNALHVLNAPSPAATASLAIGEEIVEMVAKG
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]