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The map label for this gene is oprM [H]

Identifier: 116624870

GI number: 116624870

Start: 7258345

End: 7259787

Strand: Reverse

Name: oprM [H]

Synonym: Acid_5794

Alternate gene names: 116624870

Gene position: 7259787-7258345 (Counterclockwise)

Preceding gene: 116624871

Following gene: 116624869

Centisome position: 72.85

GC content: 63.34

Gene sequence:

>1443_bases
TTGGTTGTTCTCCCCTTCTCGCTGCTTCTGCCGGCTTGCGCGGTCGGCCCCAATTACCGGCGGCCCAGCGTGAGCGCCCC
GCCGGCCTTTCGGTCCGCCGCGGGTGCGGCCCAGCAGGCCTCGTTTGCCGACCTGCCGTGGTGGGACGTCTTTAGAGATG
ACACGCTGAAGCAGCTCATAAAGACGTCGCTCGCCAATAATTACGATCTGCAAGCGGCAATCGCGCGCGTCGAACAGGCG
CGCCAGGTTGCCGCGTTGGCCCGGTCGGAATATTTTCCCGCCATCAACTATTCGACCGTCACTACCTACGGACACAATCA
GTTCATCAACAGCCCGGTATCCATTTCACCCGGCGCTCAGGGATTCCTGCTGGGGATTGCCAGCGCGGCCTGGGAGGCGG
ACGTGTGGGGCCGCATCCGCCGCACGAATGAAGGCGCACGCGCGCAATACCTGGCGACCGAGGAAGCCCGCCGCGGCGTC
ATGCTTACGCTGACCAGCGATCTCTCCCAGGCCTACTTCGAACTCCTCGGACTGCGGCTTCAACTCGATATCGCCAGGGA
AACCACGCAATCCTACACGGCAACCCTCAACCTTTTCTCCGATCGCCTGCGGGAAGGTCTTGGCAATGCATTACAGACGT
CGCGCGCCGCGGCCGATCTCGCGTCCGCCGCCGCAAGCGTGCGTGAGATCGAGCGGCAGATCGCGATCAAGGAAAATCAG
ATCAGCGTGTTGCTGGGCAAGAACCCTGGCGCGATCGAAACCAAAGCCAAGCTCCTTGAAGAGACCGTGCCGCCATCGAT
CCCCGTGGGCTTGCCTTCCGCGTTGCTGGAGCGCCGGCCGGACGTGCTCTCCGCCGAGCAAAGGGTGCGCTACGCGAACG
CCCAGATCGGCGTCGCCAAGTCGGCATACTTCCCGAGGATCGGACTCACTACGTTCTTCGGCAAATTGAGTACGCCGCTG
GCAGATCTGAGTTCCGGCAATACCAATGCCTGGAGCGTGGGTGCAAACGCCGCGGGCCCGATCTTTCGCGGCGGCGCCGT
GAGGGCTCAGCACCGCCAGGCGATCGCATTCCGGGACGAGGCCGAAGCCGAGTACCGGCAGACGGCTGTACGCGCATTTC
GGGATGTTGCCGACGCCCTGGTTTCGCGCGAGAAATACGATGCGATTCGCGAGGACCTGATCCGCGGCGTGCGATCGTCC
GAAGAGGCCGTCCGGCTTGCGCGGATGCGCTATCTCGAAGGGCTCTCCAATTACAACGAGGTTCTCGAGGCCCAGCAGCG
TCTCTATCCGTCCCAGCTTGCTCTCGCCCAGACGGAAATCAATCGCCGGCTGGTCGTGGTCCAACTCTACAAAGCCCTGG
GCGGAGGATGGAACCTGACGGATGCGGAATGGGTAACACCCAACGTCCCGCCCGCGAACCATCCGCCAGCCAAGAGACCG
TGA

Upstream 100 bases:

>100_bases
GGATTACTAAGTCTGCCCGCGGCGATGAACCACAGAGTGGAGTAGATCCCAAGCGATGACGATACGAAAAGGCTGGAAGC
GAACTGCTTCTCTTCCAGCC

Downstream 100 bases:

>100_bases
GTGAGGATCTTATGGCTAACCAACCGACCCCGCCGCGCAGTGTCAAAGAGCCATCCTATACGGATGCGATCATTCCGCTG
GTTACCCTCATCATTCTAAT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVVLPFSLLLPACAVGPNYRRPSVSAPPAFRSAAGAAQQASFADLPWWDVFRDDTLKQLIKTSLANNYDLQAAIARVEQA
RQVAALARSEYFPAINYSTVTTYGHNQFINSPVSISPGAQGFLLGIASAAWEADVWGRIRRTNEGARAQYLATEEARRGV
MLTLTSDLSQAYFELLGLRLQLDIARETTQSYTATLNLFSDRLREGLGNALQTSRAAADLASAAASVREIERQIAIKENQ
ISVLLGKNPGAIETKAKLLEETVPPSIPVGLPSALLERRPDVLSAEQRVRYANAQIGVAKSAYFPRIGLTTFFGKLSTPL
ADLSSGNTNAWSVGANAAGPIFRGGAVRAQHRQAIAFRDEAEAEYRQTAVRAFRDVADALVSREKYDAIREDLIRGVRSS
EEAVRLARMRYLEGLSNYNEVLEAQQRLYPSQLALAQTEINRRLVVVQLYKALGGGWNLTDAEWVTPNVPPANHPPAKRP

Sequences:

>Translated_480_residues
MVVLPFSLLLPACAVGPNYRRPSVSAPPAFRSAAGAAQQASFADLPWWDVFRDDTLKQLIKTSLANNYDLQAAIARVEQA
RQVAALARSEYFPAINYSTVTTYGHNQFINSPVSISPGAQGFLLGIASAAWEADVWGRIRRTNEGARAQYLATEEARRGV
MLTLTSDLSQAYFELLGLRLQLDIARETTQSYTATLNLFSDRLREGLGNALQTSRAAADLASAAASVREIERQIAIKENQ
ISVLLGKNPGAIETKAKLLEETVPPSIPVGLPSALLERRPDVLSAEQRVRYANAQIGVAKSAYFPRIGLTTFFGKLSTPL
ADLSSGNTNAWSVGANAAGPIFRGGAVRAQHRQAIAFRDEAEAEYRQTAVRAFRDVADALVSREKYDAIREDLIRGVRSS
EEAVRLARMRYLEGLSNYNEVLEAQQRLYPSQLALAQTEINRRLVVVQLYKALGGGWNLTDAEWVTPNVPPANHPPAKRP
>Mature_480_residues
MVVLPFSLLLPACAVGPNYRRPSVSAPPAFRSAAGAAQQASFADLPWWDVFRDDTLKQLIKTSLANNYDLQAAIARVEQA
RQVAALARSEYFPAINYSTVTTYGHNQFINSPVSISPGAQGFLLGIASAAWEADVWGRIRRTNEGARAQYLATEEARRGV
MLTLTSDLSQAYFELLGLRLQLDIARETTQSYTATLNLFSDRLREGLGNALQTSRAAADLASAAASVREIERQIAIKENQ
ISVLLGKNPGAIETKAKLLEETVPPSIPVGLPSALLERRPDVLSAEQRVRYANAQIGVAKSAYFPRIGLTTFFGKLSTPL
ADLSSGNTNAWSVGANAAGPIFRGGAVRAQHRQAIAFRDEAEAEYRQTAVRAFRDVADALVSREKYDAIREDLIRGVRSS
EEAVRLARMRYLEGLSNYNEVLEAQQRLYPSQLALAQTEINRRLVVVQLYKALGGGWNLTDAEWVTPNVPPANHPPAKRP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=461, Percent_Identity=30.8026030368764, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI2367348, Length=432, Percent_Identity=23.6111111111111, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52535; Mature: 52535

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVLPFSLLLPACAVGPNYRRPSVSAPPAFRSAAGAAQQASFADLPWWDVFRDDTLKQLI
CEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHH
KTSLANNYDLQAAIARVEQARQVAALARSEYFPAINYSTVTTYGHNQFINSPVSISPGAQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCCCCCCCCC
GFLLGIASAAWEADVWGRIRRTNEGARAQYLATEEARRGVMLTLTSDLSQAYFELLGLRL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEHHHHHCCEEEEEHHHHHHHHHHHHCCHH
QLDIARETTQSYTATLNLFSDRLREGLGNALQTSRAAADLASAAASVREIERQIAIKENQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ISVLLGKNPGAIETKAKLLEETVPPSIPVGLPSALLERRPDVLSAEQRVRYANAQIGVAK
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHCHHHHHHHHCCCCCCHH
SAYFPRIGLTTFFGKLSTPLADLSSGNTNAWSVGANAAGPIFRGGAVRAQHRQAIAFRDE
HCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCH
AEAEYRQTAVRAFRDVADALVSREKYDAIREDLIRGVRSSEEAVRLARMRYLEGLSNYNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VLEAQQRLYPSQLALAQTEINRRLVVVQLYKALGGGWNLTDAEWVTPNVPPANHPPAKRP
HHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVVLPFSLLLPACAVGPNYRRPSVSAPPAFRSAAGAAQQASFADLPWWDVFRDDTLKQLI
CEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHH
KTSLANNYDLQAAIARVEQARQVAALARSEYFPAINYSTVTTYGHNQFINSPVSISPGAQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCCCCCCCCC
GFLLGIASAAWEADVWGRIRRTNEGARAQYLATEEARRGVMLTLTSDLSQAYFELLGLRL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEHHHHHCCEEEEEHHHHHHHHHHHHCCHH
QLDIARETTQSYTATLNLFSDRLREGLGNALQTSRAAADLASAAASVREIERQIAIKENQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ISVLLGKNPGAIETKAKLLEETVPPSIPVGLPSALLERRPDVLSAEQRVRYANAQIGVAK
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHCHHHHHHHHCCCCCCHH
SAYFPRIGLTTFFGKLSTPLADLSSGNTNAWSVGANAAGPIFRGGAVRAQHRQAIAFRDE
HCCCCCCHHHHHHHHHCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCH
AEAEYRQTAVRAFRDVADALVSREKYDAIREDLIRGVRSSEEAVRLARMRYLEGLSNYNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VLEAQQRLYPSQLALAQTEINRRLVVVQLYKALGGGWNLTDAEWVTPNVPPANHPPAKRP
HHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]