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The map label for this gene is nuoI2

Identifier: 116624761

GI number: 116624761

Start: 7122255

End: 7122710

Strand: Direct

Name: nuoI2

Synonym: Acid_5685

Alternate gene names: 116624761

Gene position: 7122255-7122710 (Clockwise)

Preceding gene: 116624760

Following gene: 116624762

Centisome position: 71.47

GC content: 58.11

Gene sequence:

>456_bases
ATGATTGAGTTGCTGAAAAAGATCTTCCTGGTGGATCTGTTCCAGGGTCTCTTCGTGACCTTCCGGAACCAGAACCCGAA
GTACATTTACACGGAGCAGTATCCCGCCGAGCGGCCCAAGGTCGCCGAGCGGTACCGTGGCGCTCCGCGACTCAACATCA
ATCCCGATAATGGCGAAACGCTTTGCATCAGTTGCAATTTGTGCGCGCTGGCCTGCCCGGAGAACCTGATCGTGGTGACC
AGCGAGCGCAGTGAGGTCACCAAGCGCAAAGAGTTGGTCACCTTCACGTACGATACTTCACGCTGCATGTTCTGCGGGCT
GTGCGAGGACGCCTGCCCCGTGGACGCGCTGGAATTGACGCAGGATTTTGAAATGGCCAGCTACACGCGCGAAGGCCAGA
TATGGGACCGGCAGGCGCTCGAAGAAGGGCCGCGGCCGACGCAATACAAATGCTGA

Upstream 100 bases:

>100_bases
CAGGCGTTGGACCGGATGATCCGCGGCGGGCTGATCGCGGATGTGGTGGCGGTGATCGGGACGCTCGACATCGTCTTGGG
CGAGATTGACCGGTGAACTT

Downstream 100 bases:

>100_bases
CCGATACGATTCTGTTCTACATCTTTGCCGTGATGGTGCTGGCGGGCGGCATCCTGACCATCACGCGGCGCTCCGCGGTG
CACAGCGCGATCTCGCTGAT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I 2; NDH-1 subunit I 2

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MIELLKKIFLVDLFQGLFVTFRNQNPKYIYTEQYPAERPKVAERYRGAPRLNINPDNGETLCISCNLCALACPENLIVVT
SERSEVTKRKELVTFTYDTSRCMFCGLCEDACPVDALELTQDFEMASYTREGQIWDRQALEEGPRPTQYKC

Sequences:

>Translated_151_residues
MIELLKKIFLVDLFQGLFVTFRNQNPKYIYTEQYPAERPKVAERYRGAPRLNINPDNGETLCISCNLCALACPENLIVVT
SERSEVTKRKELVTFTYDTSRCMFCGLCEDACPVDALELTQDFEMASYTREGQIWDRQALEEGPRPTQYKC
>Mature_151_residues
MIELLKKIFLVDLFQGLFVTFRNQNPKYIYTEQYPAERPKVAERYRGAPRLNINPDNGETLCISCNLCALACPENLIVVT
SERSEVTKRKELVTFTYDTSRCMFCGLCEDACPVDALELTQDFEMASYTREGQIWDRQALEEGPRPTQYKC

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=140, Percent_Identity=31.4285714285714, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1788617, Length=110, Percent_Identity=42.7272727272727, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1789075, Length=101, Percent_Identity=32.6732673267327, Blast_Score=63, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=139, Percent_Identity=33.0935251798561, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17864306, Length=136, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI2_SOLUE (Q01UN8)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_826917.1
- ProteinModelPortal:   Q01UN8
- SMR:   Q01UN8
- STRING:   Q01UN8
- GeneID:   4426095
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_5685
- NMPDR:   fig|234267.9.peg.5408
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   QWEVEIS
- PhylomeDB:   Q01UN8
- ProtClustDB:   CLSK774499
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 17438; Mature: 17438

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
6.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELLKKIFLVDLFQGLFVTFRNQNPKYIYTEQYPAERPKVAERYRGAPRLNINPDNGET
CHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCCCHHHHHHCCCCEEEECCCCCCE
LCISCNLCALACPENLIVVTSERSEVTKRKELVTFTYDTSRCMFCGLCEDACPVDALELT
EEEECCEEEEECCCCEEEEECCHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCCHHHHH
QDFEMASYTREGQIWDRQALEEGPRPTQYKC
HHHHHHHHCCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIELLKKIFLVDLFQGLFVTFRNQNPKYIYTEQYPAERPKVAERYRGAPRLNINPDNGET
CHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCCCCCCCHHHHHHCCCCEEEECCCCCCE
LCISCNLCALACPENLIVVTSERSEVTKRKELVTFTYDTSRCMFCGLCEDACPVDALELT
EEEECCEEEEECCCCEEEEECCHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCCHHHHH
QDFEMASYTREGQIWDRQALEEGPRPTQYKC
HHHHHHHHCCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA