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The map label for this gene is asnB [H]

Identifier: 116624686

GI number: 116624686

Start: 7026540

End: 7028438

Strand: Reverse

Name: asnB [H]

Synonym: Acid_5610

Alternate gene names: 116624686

Gene position: 7028438-7026540 (Counterclockwise)

Preceding gene: 116624687

Following gene: 116624683

Centisome position: 70.53

GC content: 63.4

Gene sequence:

>1899_bases
ATGTGCGGGATTGTTGCTGTCTACGATGCCGAAGGCGCGGGAGCGTCGGCCGAAGTTGCGGCCGGGTTGAAAACACTGCG
CCATCGAGGGCCCGATGCGCAGCATGTCTGGCTATCGCCGGATGGAGCAATCTGCCTCGGCCACGCCAGGCTGAGCATCA
TCGACCTGGCGCACGGCGACCAGCCGCTGGCCAACGAAGACGAGTCGATTCACTGCGTGGTGAACGGCGAGTTTTACGAT
TTCGAGAGAATTCGCCGCGGGCTGGAAGGGCGCGGTCATCGTTTCCGCACCCACAGCGATAGCGAGATCCTGCTGCACCT
TTACGAAGATCTCGGCGTGGAGTGCCTGCGCGAACTGCGCGGCGAATTTGCTTTCGTGCTGTGGGACGGACGCCGGCGAG
TTCTGATGGCGGCGCGCGACCGTTTCGGCATCAAGCCGCTATTCTATTCGCGGACCGGCGAGCGGCTACTTTTCGCCTCG
GAGGTGAAGGCGCTGCATGCCGCGGGCGTCCCGGCGGCCTGGGACGAGCAGGGCTTCCTCGAGACGTTCTCGGCGATGTA
CGCGAGCTCGCCTACGCTCTTCCGTCACGTTTCCGCGATTCCGCCGGGACACTATCTGCTGTCTCGCGATTTGGGCTGCT
CGCTGCACAAGTACTGGGATTTCGAATTTCCGGCGGAAGGGAATCTACCCGAGCGGCGCGACGAAGAGTGCGTCGCGGAG
TTCGGCGCGGTGCTGGATGAGGCGGTGCGGCTGCGCCTGCGCGCCGACGTTCCGGTGGGCTGCTATCTGAGCGGCGGCAT
CGATTCGTGCAGCGTGCTGGCGCTCATGGCGAAGCACGCTCCCGGCAGGGTGCGCGCTTTTTCCCTGAGCTTCGATCATG
AACTCTACGACGAAGGGCCGATCGCGCGCGAGATGGCCCAACGGACCGGCGCCGAGTTCACCATGATCCCGGTGAGCCAC
GCCGGTATGGCGGCCGATTTGAGCGATGCCCTCTGGCACAACGAGAGCTTCTTCCTGAATGCCGGCAGCGCGGCCAAGTT
CGCGCTCAGCCGCGCGGTGCGCGATGCAGGCTACAAGGTGGTGCTCACCGGCGAAGGCTCCGATGAGGTGCTGGCAGGAT
ATGCGTTCTTCCGGCAGGATATGATCCGGCACGCCAGTAACAACGGTGGGGCCGCCGAGGCGATTGAACGGCTGCGCGCC
GCCAACCCTGCTTCCGGCGGCCTTATGTTTACGACGAAGCAGGTGACGCCGGTGGCCGGTTACGTGGATCGCCTCGGATA
TTTCCCGAGCTTCCTGGAGGCGCGCAAGGCGGGGCTCGAAGCGCTGCGGGCGCTGCTGACGATTGCCTACGAAACCGATG
AAGTGCTGACGCGCCTGCTCAACCGCGTCGATATCGCGGGACAATTGACGGGCCGGCACGTACTCAACCAGAGCTTGTAT
CTGAACAACAAAACGATCCTGCCGGGATATATTCTCTCGGCGCTCGGAGATCGCGTCGAGATGGCGCACTCGATTGAGGC
GAGGCTTCCATTCCTGGATCATCACGTGGTGGAGTACACGCGCAGGATTCCAGTTGACCTGAAGATTCGCGGCACGGTGG
AGAAGTACATTTTGCGCGAGGCGATGAAGCCGATGCTGACCACGACGGTCTATAAACGGCAGAAGCATCCGTTTCTTTCG
CCGCCCGCGTTGTTGAAGCCGGATGAGCCGCTGCACGCGCTGTTGCAGGACACTCTGCGCGGGCGGGCACTCGACCGCGT
GCCGTACCTGCGACGGACGGAAGTCGCGGGATTTCTGGACCGGGCGGGGGGACTGGATGTGGCTGGAAAAATCGCGGCGG
AGTTCCCGCTGATGATGATGCTGAGCGCGTGCCTGGTTGCGGAGCGATTCGGGTTGTAG

Upstream 100 bases:

>100_bases
GCCCGTCGTAATTTCGGTCGGCGGCCTGCCGAGTCCGGCGGCGACCGTGACGGTGCAGTAGTACACTGGGTGAGTTTGCA
TTGAGGGGCTCACTCGAATT

Downstream 100 bases:

>100_bases
GGCTATAGGGTGGCTCTCACCGGCAAGGCGAACTGGGCGTTCTCGAAGAGGGGCGGCCGTGTTGGCGCGGGGAAGCCGGC
TGAAAGCCGGCTGCAGCCAG

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MCGIVAVYDAEGAGASAEVAAGLKTLRHRGPDAQHVWLSPDGAICLGHARLSIIDLAHGDQPLANEDESIHCVVNGEFYD
FERIRRGLEGRGHRFRTHSDSEILLHLYEDLGVECLRELRGEFAFVLWDGRRRVLMAARDRFGIKPLFYSRTGERLLFAS
EVKALHAAGVPAAWDEQGFLETFSAMYASSPTLFRHVSAIPPGHYLLSRDLGCSLHKYWDFEFPAEGNLPERRDEECVAE
FGAVLDEAVRLRLRADVPVGCYLSGGIDSCSVLALMAKHAPGRVRAFSLSFDHELYDEGPIAREMAQRTGAEFTMIPVSH
AGMAADLSDALWHNESFFLNAGSAAKFALSRAVRDAGYKVVLTGEGSDEVLAGYAFFRQDMIRHASNNGGAAEAIERLRA
ANPASGGLMFTTKQVTPVAGYVDRLGYFPSFLEARKAGLEALRALLTIAYETDEVLTRLLNRVDIAGQLTGRHVLNQSLY
LNNKTILPGYILSALGDRVEMAHSIEARLPFLDHHVVEYTRRIPVDLKIRGTVEKYILREAMKPMLTTTVYKRQKHPFLS
PPALLKPDEPLHALLQDTLRGRALDRVPYLRRTEVAGFLDRAGGLDVAGKIAAEFPLMMMLSACLVAERFGL

Sequences:

>Translated_632_residues
MCGIVAVYDAEGAGASAEVAAGLKTLRHRGPDAQHVWLSPDGAICLGHARLSIIDLAHGDQPLANEDESIHCVVNGEFYD
FERIRRGLEGRGHRFRTHSDSEILLHLYEDLGVECLRELRGEFAFVLWDGRRRVLMAARDRFGIKPLFYSRTGERLLFAS
EVKALHAAGVPAAWDEQGFLETFSAMYASSPTLFRHVSAIPPGHYLLSRDLGCSLHKYWDFEFPAEGNLPERRDEECVAE
FGAVLDEAVRLRLRADVPVGCYLSGGIDSCSVLALMAKHAPGRVRAFSLSFDHELYDEGPIAREMAQRTGAEFTMIPVSH
AGMAADLSDALWHNESFFLNAGSAAKFALSRAVRDAGYKVVLTGEGSDEVLAGYAFFRQDMIRHASNNGGAAEAIERLRA
ANPASGGLMFTTKQVTPVAGYVDRLGYFPSFLEARKAGLEALRALLTIAYETDEVLTRLLNRVDIAGQLTGRHVLNQSLY
LNNKTILPGYILSALGDRVEMAHSIEARLPFLDHHVVEYTRRIPVDLKIRGTVEKYILREAMKPMLTTTVYKRQKHPFLS
PPALLKPDEPLHALLQDTLRGRALDRVPYLRRTEVAGFLDRAGGLDVAGKIAAEFPLMMMLSACLVAERFGL
>Mature_632_residues
MCGIVAVYDAEGAGASAEVAAGLKTLRHRGPDAQHVWLSPDGAICLGHARLSIIDLAHGDQPLANEDESIHCVVNGEFYD
FERIRRGLEGRGHRFRTHSDSEILLHLYEDLGVECLRELRGEFAFVLWDGRRRVLMAARDRFGIKPLFYSRTGERLLFAS
EVKALHAAGVPAAWDEQGFLETFSAMYASSPTLFRHVSAIPPGHYLLSRDLGCSLHKYWDFEFPAEGNLPERRDEECVAE
FGAVLDEAVRLRLRADVPVGCYLSGGIDSCSVLALMAKHAPGRVRAFSLSFDHELYDEGPIAREMAQRTGAEFTMIPVSH
AGMAADLSDALWHNESFFLNAGSAAKFALSRAVRDAGYKVVLTGEGSDEVLAGYAFFRQDMIRHASNNGGAAEAIERLRA
ANPASGGLMFTTKQVTPVAGYVDRLGYFPSFLEARKAGLEALRALLTIAYETDEVLTRLLNRVDIAGQLTGRHVLNQSLY
LNNKTILPGYILSALGDRVEMAHSIEARLPFLDHHVVEYTRRIPVDLKIRGTVEKYILREAMKPMLTTTVYKRQKHPFLS
PPALLKPDEPLHALLQDTLRGRALDRVPYLRRTEVAGFLDRAGGLDVAGKIAAEFPLMMMLSACLVAERFGL

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=411, Percent_Identity=27.7372262773723, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI168229252, Length=411, Percent_Identity=27.7372262773723, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI168229250, Length=411, Percent_Identity=27.7372262773723, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI296010848, Length=386, Percent_Identity=27.720207253886, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI296010852, Length=315, Percent_Identity=27.9365079365079, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI296010850, Length=315, Percent_Identity=27.9365079365079, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786889, Length=400, Percent_Identity=32.5, Blast_Score=164, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71993933, Length=419, Percent_Identity=28.1622911694511, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=419, Percent_Identity=28.1622911694511, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=330, Percent_Identity=27.5757575757576, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321563, Length=422, Percent_Identity=28.6729857819905, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325403, Length=423, Percent_Identity=28.3687943262411, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI45553209, Length=502, Percent_Identity=27.0916334661355, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69904; Mature: 69904

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVAVYDAEGAGASAEVAAGLKTLRHRGPDAQHVWLSPDGAICLGHARLSIIDLAHGD
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCEEEEEEECCCC
QPLANEDESIHCVVNGEFYDFERIRRGLEGRGHRFRTHSDSEILLHLYEDLGVECLRELR
CCCCCCCCEEEEEECCCEECHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHC
GEFAFVLWDGRRRVLMAARDRFGIKPLFYSRTGERLLFASEVKALHAAGVPAAWDEQGFL
CCEEEEEECCCCEEEEEHHHCCCCCEEEECCCCCEEEEHHHHHHHHHCCCCCCCCCCHHH
ETFSAMYASSPTLFRHVSAIPPGHYLLSRDLGCSLHKYWDFEFPAEGNLPERRDEECVAE
HHHHHHHCCCCHHHHHHHCCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHH
FGAVLDEAVRLRLRADVPVGCYLSGGIDSCSVLALMAKHAPGRVRAFSLSFDHELYDEGP
HHHHHHHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHCCCCH
IAREMAQRTGAEFTMIPVSHAGMAADLSDALWHNESFFLNAGSAAKFALSRAVRDAGYKV
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCEE
VLTGEGSDEVLAGYAFFRQDMIRHASNNGGAAEAIERLRAANPASGGLMFTTKQVTPVAG
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
YVDRLGYFPSFLEARKAGLEALRALLTIAYETDEVLTRLLNRVDIAGQLTGRHVLNQSLY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LNNKTILPGYILSALGDRVEMAHSIEARLPFLDHHVVEYTRRIPVDLKIRGTVEKYILRE
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
AMKPMLTTTVYKRQKHPFLSPPALLKPDEPLHALLQDTLRGRALDRVPYLRRTEVAGFLD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHH
RAGGLDVAGKIAAEFPLMMMLSACLVAERFGL
CCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIVAVYDAEGAGASAEVAAGLKTLRHRGPDAQHVWLSPDGAICLGHARLSIIDLAHGD
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCEEEEEEECCCC
QPLANEDESIHCVVNGEFYDFERIRRGLEGRGHRFRTHSDSEILLHLYEDLGVECLRELR
CCCCCCCCEEEEEECCCEECHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHC
GEFAFVLWDGRRRVLMAARDRFGIKPLFYSRTGERLLFASEVKALHAAGVPAAWDEQGFL
CCEEEEEECCCCEEEEEHHHCCCCCEEEECCCCCEEEEHHHHHHHHHCCCCCCCCCCHHH
ETFSAMYASSPTLFRHVSAIPPGHYLLSRDLGCSLHKYWDFEFPAEGNLPERRDEECVAE
HHHHHHHCCCCHHHHHHHCCCCCCHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHH
FGAVLDEAVRLRLRADVPVGCYLSGGIDSCSVLALMAKHAPGRVRAFSLSFDHELYDEGP
HHHHHHHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCHHCCCCH
IAREMAQRTGAEFTMIPVSHAGMAADLSDALWHNESFFLNAGSAAKFALSRAVRDAGYKV
HHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCEE
VLTGEGSDEVLAGYAFFRQDMIRHASNNGGAAEAIERLRAANPASGGLMFTTKQVTPVAG
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
YVDRLGYFPSFLEARKAGLEALRALLTIAYETDEVLTRLLNRVDIAGQLTGRHVLNQSLY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LNNKTILPGYILSALGDRVEMAHSIEARLPFLDHHVVEYTRRIPVDLKIRGTVEKYILRE
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHH
AMKPMLTTTVYKRQKHPFLSPPALLKPDEPLHALLQDTLRGRALDRVPYLRRTEVAGFLD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHH
RAGGLDVAGKIAAEFPLMMMLSACLVAERFGL
CCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]