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The map label for this gene is mdeA [H]

Identifier: 116624563

GI number: 116624563

Start: 6880974

End: 6882287

Strand: Reverse

Name: mdeA [H]

Synonym: Acid_5487

Alternate gene names: 116624563

Gene position: 6882287-6880974 (Counterclockwise)

Preceding gene: 116624566

Following gene: 116624558

Centisome position: 69.06

GC content: 60.73

Gene sequence:

>1314_bases
ATGCCAGATAACAACGCTCCCGACCGCGAACTAGGTTTCGCCACGAAGTCCCTGCACGCCGGCCACACACCGGACGCCTC
GAGCCGCGCCCGAGCGGTTCCGATTTATCAATCGACATCGTTCACTTTTGAGAACTCCCAGCACGCGGCAGATCTCTTTG
CCCTGCAGAAGTTCGGCAATATCTATAGCCGCATCATGAACCCGACCACGGATGTTTTCGAGCAGCGCGTGGCCGCGCTC
GAAGGCGGCATGGCGGGTCTGGCGACGGCCAGCGGACAGGCGGCGCAATTTCTCGCCATCACCAGCCTGCTTGGTCCCGG
CGATGAACTGGTTGCGGCCAGCACACTCTATGGCGGTACCTACACGCAGATGGACGTGAGCTTCCGGCGTCTCGGGATCG
ACGTGAAGTTCGTGGAGCCCGACGATCCGGAGAACTTCCGCAAGGCGATCACCTCGAAAACTCGCGCGATTTATGGCGAG
ACCATCGCCAACCCGCGTATGAACGTGCTCGATATCGAAGCGGTGAGCAAGATCGCGCACGAGGCGGGAGTGCCGCTGGT
GATCGACAACACTATGGCATCGCCTTATCTCTGCCGTCCGATCGAGCACGGCGCGGATATCGTGGTGCACTCTGCCACCA
AGTTCCTGGGCGGACACGGCACGTCGATCGGCGGCATCATCGTGGATAGCGGCAAGTTCCCGTGGAGCGATAAGTTCCCG
GCGATTACGCAGCCGAGCCCCGGCTATCACGGCATGAAGTTCAGCGAAGTCTTCGGACCGCTCGCGTACATCATCAAGGT
GCGCGTGGAAGGCCTGCGCGATTTCGGACCCTGCATCAGCCCCTTCAATTCCTTCCTCTTCATTCAGGGAATCGAGACCC
TGAAGTTCCGGATGGAGGCTCACTGCTCCAACGCGCTGGCGGTAGCCGAGTGGCTCGGCAAGCAGCCCGCGGTGACCTGG
GTGAAGTATCCCGGCCTCAAGTCGAGCCCGTATTACGCGCTCTCGCAGAAGTACCTGCCGAAGGGCCAAGGCTCGATTGT
GACGTTCGGGATCAAGGGCGGCCTGGAGGCAGGCAAGAAGCTAATCGACAACGCCAAGATCTTCTCGCACCTCGCCAACC
TCGGCGATGCCAAGAGCCTGATCATTCATCCCGCCTCAACCACCCACCAACAGTTGAGCGACGAGCAGCAGTTGGAAGCG
GGCGTGACAAAAGACCTGGTGAGACTCTCGGTGGGAATCGAAGACGTCGAAGATTTGATCTGGGACCTGGGGCAGGCGAT
CGAAGCGTCGCAGGCCAGCGCAGCTTCCGCGTAA

Upstream 100 bases:

>100_bases
CGAAGATCTTCGTCATTGTCAACGAAAACTTTCGTCAAGCTATGGCCGGAAGACAAATGTGTGAACTGATAACGCTGGTG
CGTTTAGTATCCTGTAGCTT

Downstream 100 bases:

>100_bases
ATTAAATAACGAGCCGCGGTCCCTTGCGTAACCACCGGGGCCGCGGCTCGCGTCTCGTGTTACTTCCCCACAACCAGAAT
ATGGAAGCTGTCCGGCAGCA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MPDNNAPDRELGFATKSLHAGHTPDASSRARAVPIYQSTSFTFENSQHAADLFALQKFGNIYSRIMNPTTDVFEQRVAAL
EGGMAGLATASGQAAQFLAITSLLGPGDELVAASTLYGGTYTQMDVSFRRLGIDVKFVEPDDPENFRKAITSKTRAIYGE
TIANPRMNVLDIEAVSKIAHEAGVPLVIDNTMASPYLCRPIEHGADIVVHSATKFLGGHGTSIGGIIVDSGKFPWSDKFP
AITQPSPGYHGMKFSEVFGPLAYIIKVRVEGLRDFGPCISPFNSFLFIQGIETLKFRMEAHCSNALAVAEWLGKQPAVTW
VKYPGLKSSPYYALSQKYLPKGQGSIVTFGIKGGLEAGKKLIDNAKIFSHLANLGDAKSLIIHPASTTHQQLSDEQQLEA
GVTKDLVRLSVGIEDVEDLIWDLGQAIEASQASAASA

Sequences:

>Translated_437_residues
MPDNNAPDRELGFATKSLHAGHTPDASSRARAVPIYQSTSFTFENSQHAADLFALQKFGNIYSRIMNPTTDVFEQRVAAL
EGGMAGLATASGQAAQFLAITSLLGPGDELVAASTLYGGTYTQMDVSFRRLGIDVKFVEPDDPENFRKAITSKTRAIYGE
TIANPRMNVLDIEAVSKIAHEAGVPLVIDNTMASPYLCRPIEHGADIVVHSATKFLGGHGTSIGGIIVDSGKFPWSDKFP
AITQPSPGYHGMKFSEVFGPLAYIIKVRVEGLRDFGPCISPFNSFLFIQGIETLKFRMEAHCSNALAVAEWLGKQPAVTW
VKYPGLKSSPYYALSQKYLPKGQGSIVTFGIKGGLEAGKKLIDNAKIFSHLANLGDAKSLIIHPASTTHQQLSDEQQLEA
GVTKDLVRLSVGIEDVEDLIWDLGQAIEASQASAASA
>Mature_436_residues
PDNNAPDRELGFATKSLHAGHTPDASSRARAVPIYQSTSFTFENSQHAADLFALQKFGNIYSRIMNPTTDVFEQRVAALE
GGMAGLATASGQAAQFLAITSLLGPGDELVAASTLYGGTYTQMDVSFRRLGIDVKFVEPDDPENFRKAITSKTRAIYGET
IANPRMNVLDIEAVSKIAHEAGVPLVIDNTMASPYLCRPIEHGADIVVHSATKFLGGHGTSIGGIIVDSGKFPWSDKFPA
ITQPSPGYHGMKFSEVFGPLAYIIKVRVEGLRDFGPCISPFNSFLFIQGIETLKFRMEAHCSNALAVAEWLGKQPAVTWV
KYPGLKSSPYYALSQKYLPKGQGSIVTFGIKGGLEAGKKLIDNAKIFSHLANLGDAKSLIIHPASTTHQQLSDEQQLEAG
VTKDLVRLSVGIEDVEDLIWDLGQAIEASQASAASA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=35.3629976580796, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=32.0843091334895, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=30.9859154929577, Blast_Score=148, Evalue=9e-36,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=31.6666666666667, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789383, Length=417, Percent_Identity=25.1798561151079, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538055, Length=407, Percent_Identity=33.6609336609337, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=406, Percent_Identity=33.0049261083744, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=438, Percent_Identity=30.8219178082192, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=53.4722222222222, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=34.51536643026, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=438, Percent_Identity=28.5388127853881, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=364, Percent_Identity=24.7252747252747, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=428, Percent_Identity=24.5327102803738, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922554, Length=428, Percent_Identity=35.2803738317757, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47034; Mature: 46903

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNNAPDRELGFATKSLHAGHTPDASSRARAVPIYQSTSFTFENSQHAADLFALQKFGN
CCCCCCCCHHHCCHHHHCCCCCCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHH
IYSRIMNPTTDVFEQRVAALEGGMAGLATASGQAAQFLAITSLLGPGDELVAASTLYGGT
HHHHHCCCHHHHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHCCCCHHHEEEHEECCCE
YTQMDVSFRRLGIDVKFVEPDDPENFRKAITSKTRAIYGETIANPRMNVLDIEAVSKIAH
EEEHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
EAGVPLVIDNTMASPYLCRPIEHGADIVVHSATKFLGGHGTSIGGIIVDSGKFPWSDKFP
HCCCCEEEECCCCCCCEECCCCCCCCEEEECHHHHHCCCCCCCCEEEEECCCCCCCCCCC
AITQPSPGYHGMKFSEVFGPLAYIIKVRVEGLRDFGPCISPFNSFLFIQGIETLKFRMEA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEECHHHHHHHHHH
HCSNALAVAEWLGKQPAVTWVKYPGLKSSPYYALSQKYLPKGQGSIVTFGIKGGLEAGKK
HHCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCHHHHHH
LIDNAKIFSHLANLGDAKSLIIHPASTTHQQLSDEQQLEAGVTKDLVRLSVGIEDVEDLI
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
WDLGQAIEASQASAASA
HHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
PDNNAPDRELGFATKSLHAGHTPDASSRARAVPIYQSTSFTFENSQHAADLFALQKFGN
CCCCCCCHHHCCHHHHCCCCCCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHH
IYSRIMNPTTDVFEQRVAALEGGMAGLATASGQAAQFLAITSLLGPGDELVAASTLYGGT
HHHHHCCCHHHHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHCCCCHHHEEEHEECCCE
YTQMDVSFRRLGIDVKFVEPDDPENFRKAITSKTRAIYGETIANPRMNVLDIEAVSKIAH
EEEHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHH
EAGVPLVIDNTMASPYLCRPIEHGADIVVHSATKFLGGHGTSIGGIIVDSGKFPWSDKFP
HCCCCEEEECCCCCCCEECCCCCCCCEEEECHHHHHCCCCCCCCEEEEECCCCCCCCCCC
AITQPSPGYHGMKFSEVFGPLAYIIKVRVEGLRDFGPCISPFNSFLFIQGIETLKFRMEA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEEECHHHHHHHHHH
HCSNALAVAEWLGKQPAVTWVKYPGLKSSPYYALSQKYLPKGQGSIVTFGIKGGLEAGKK
HHCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCHHHHHH
LIDNAKIFSHLANLGDAKSLIIHPASTTHQQLSDEQQLEAGVTKDLVRLSVGIEDVEDLI
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHH
WDLGQAIEASQASAASA
HHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]