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The map label for this gene is gne [H]

Identifier: 116624527

GI number: 116624527

Start: 6829562

End: 6830527

Strand: Reverse

Name: gne [H]

Synonym: Acid_5451

Alternate gene names: 116624527

Gene position: 6830527-6829562 (Counterclockwise)

Preceding gene: 116624528

Following gene: 116624526

Centisome position: 68.54

GC content: 66.05

Gene sequence:

>966_bases
ATGATTCTGGTGACTGGCGGTACCGGCTTCATCGGTACGCACCTGCTGGAACGCCTGGTTGCCACGAATGCGCCTGTGCG
GGCGCTGGTGCGCCCCACGAAGGCACCGCGGACACTTCCCATCGGAGTTGAGACCGTTTACGGCGACTTGGCGACAGGCG
TGGGGATCACGGCCGCGCTCGAAGGCGTGGAGACCGTGATCCATCTTGCGGGCATTACCAAGGCCTTGCACACGGACGAT
TACTACTCGGGCAACGTGCGCGCCACCGAAAAGCTGGCGCACGCGATGGCGGGGCGTGGAATGCGCATGGTGCATGTCAG
TTCGCTGGCGGCGATCGGCCCGGCCAGCGACGGGCTGCCGGTCAAGGAAGATGCAGAGCCGCACCCGTTAACGCACTACG
GGAAATCCAAGCTGGATGCGGAAGGCGTGGTGCGGGACCTGGCGCCGGACGCGGTAATCGTGCGGCCGCCGGTGGTCTAC
GGTCCGCGCGATACCGATGTTTTCCAACTGTTGAAATCGATCTCGAAGGGACTGGTGCTGGAGATCGCCGGGGGCGAGCG
CTGGTTCAGCGCGATCTTCGTGAAAGATCTGGTGGAAGGTCTGATGATGGCGGTGCGCACGCCGGGCGCAGCGGGACGGT
CCTATTTCCTGGCCCATGCCAAGCCGGTCTCGTGGACGCAACTGGGCTCGGCCGCGGCGGCCATCATGGCGCGCACGCCG
CGGGTGGTGACGGTGCCGTTCGCAGTGGCGAACGCGGTCGGGGCCTGCGGCGAAGCGTGGTCGCGGATCACGGGCAAGCC
GGGGATCCTCTCGCGCGAGAAGGTGCGGGAGGCGCGCTGCATGGCGTGGACCTGCGATACGCACCGGGCAGCCGAAGAGT
TGGGATTTGTAGCCTCCACCTCCCTGGAAGTGGGCTTGCGCGAGAGTCTGGCGTGGTATAAGGAGGCCGGATGGCTCGCG
TACTGA

Upstream 100 bases:

>100_bases
CGCGCGTGAAGGGGCTGCGCCTTCAGATCAATTCCCAACATACGGGCACGGTCGCCGAGAGCTATTTCGGGGAAGTTGCC
TTCCGTAGCATCCAGCAGTA

Downstream 100 bases:

>100_bases
AAGCCGATACTCGCTTCTGGGCCGTGGATAAGATCATCCTTGCCTACTTCGCCTTCGCGGTGGTGGTCCTGATATTTTGG
TTTAGCCGGATCGAGGACGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: UDP-GlcNAc 4-epimerase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MILVTGGTGFIGTHLLERLVATNAPVRALVRPTKAPRTLPIGVETVYGDLATGVGITAALEGVETVIHLAGITKALHTDD
YYSGNVRATEKLAHAMAGRGMRMVHVSSLAAIGPASDGLPVKEDAEPHPLTHYGKSKLDAEGVVRDLAPDAVIVRPPVVY
GPRDTDVFQLLKSISKGLVLEIAGGERWFSAIFVKDLVEGLMMAVRTPGAAGRSYFLAHAKPVSWTQLGSAAAAIMARTP
RVVTVPFAVANAVGACGEAWSRITGKPGILSREKVREARCMAWTCDTHRAAEELGFVASTSLEVGLRESLAWYKEAGWLA
Y

Sequences:

>Translated_321_residues
MILVTGGTGFIGTHLLERLVATNAPVRALVRPTKAPRTLPIGVETVYGDLATGVGITAALEGVETVIHLAGITKALHTDD
YYSGNVRATEKLAHAMAGRGMRMVHVSSLAAIGPASDGLPVKEDAEPHPLTHYGKSKLDAEGVVRDLAPDAVIVRPPVVY
GPRDTDVFQLLKSISKGLVLEIAGGERWFSAIFVKDLVEGLMMAVRTPGAAGRSYFLAHAKPVSWTQLGSAAAAIMARTP
RVVTVPFAVANAVGACGEAWSRITGKPGILSREKVREARCMAWTCDTHRAAEELGFVASTSLEVGLRESLAWYKEAGWLA
Y
>Mature_321_residues
MILVTGGTGFIGTHLLERLVATNAPVRALVRPTKAPRTLPIGVETVYGDLATGVGITAALEGVETVIHLAGITKALHTDD
YYSGNVRATEKLAHAMAGRGMRMVHVSSLAAIGPASDGLPVKEDAEPHPLTHYGKSKLDAEGVVRDLAPDAVIVRPPVVY
GPRDTDVFQLLKSISKGLVLEIAGGERWFSAIFVKDLVEGLMMAVRTPGAAGRSYFLAHAKPVSWTQLGSAAAAIMARTP
RVVTVPFAVANAVGACGEAWSRITGKPGILSREKVREARCMAWTCDTHRAAEELGFVASTSLEVGLRESLAWYKEAGWLA
Y

Specific function: Converts UDP-GlcNAc to UDP-GalNAc. Essential for O antigen synthesis [H]

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=258, Percent_Identity=23.6434108527132, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI8393516, Length=258, Percent_Identity=23.6434108527132, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI87081792, Length=334, Percent_Identity=25.748502994012, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786974, Length=343, Percent_Identity=26.2390670553936, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.7 [H]

Molecular weight: Translated: 34088; Mature: 34088

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGTGFIGTHLLERLVATNAPVRALVRPTKAPRTLPIGVETVYGDLATGVGITAAL
CEEEECCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
EGVETVIHLAGITKALHTDDYYSGNVRATEKLAHAMAGRGMRMVHVSSLAAIGPASDGLP
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCC
VKEDAEPHPLTHYGKSKLDAEGVVRDLAPDAVIVRPPVVYGPRDTDVFQLLKSISKGLVL
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHCCCEEE
EIAGGERWFSAIFVKDLVEGLMMAVRTPGAAGRSYFLAHAKPVSWTQLGSAAAAIMARTP
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
RVVTVPFAVANAVGACGEAWSRITGKPGILSREKVREARCMAWTCDTHRAAEELGFVAST
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEECCHHHHHHHHCCHHCC
SLEVGLRESLAWYKEAGWLAY
CHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MILVTGGTGFIGTHLLERLVATNAPVRALVRPTKAPRTLPIGVETVYGDLATGVGITAAL
CEEEECCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
EGVETVIHLAGITKALHTDDYYSGNVRATEKLAHAMAGRGMRMVHVSSLAAIGPASDGLP
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCC
VKEDAEPHPLTHYGKSKLDAEGVVRDLAPDAVIVRPPVVYGPRDTDVFQLLKSISKGLVL
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHCCCEEE
EIAGGERWFSAIFVKDLVEGLMMAVRTPGAAGRSYFLAHAKPVSWTQLGSAAAAIMARTP
EECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
RVVTVPFAVANAVGACGEAWSRITGKPGILSREKVREARCMAWTCDTHRAAEELGFVAST
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEECCHHHHHHHHCCHHCC
SLEVGLRESLAWYKEAGWLAY
CHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11976290; 11206551; 11258796 [H]